Ube4b (NM_001271198) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ufd2a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217526 representing NM_001271198 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGCTGAGCGCTGACGAGATTCGACGGAGGCGCCTGGCACGACTTGCTGGTGGACAGACCTCCC AGCCGACCACCCCGCTTACATCTCCCCAGAGGGAGAACCCTCCAGGACCTCCAATAGCTGCATCAGTCCC AGGCCCCTCCCAGAGCCTTGGTCTCAATGTCCACAACACGACCCCAGCTACCTCCCCCATAGGTGCAGCA GGAGTTGCCCATCGGAGCCAGAGCAGTGAAGGCGTCAGTTCTCTTAGCAGCTCACCTTCCAACAGCCTTG AGACGCAATCTCAGTCCCTCTCACGTTCCCAGAGCATGGATATCGATGGTGTCTCCTGTGAGAAGAGCAT GTCCCAGGTGGATGTGGATTCTGGAATCGAGAATATGGAGGTGGACGAGAACGACCGAAGAGAAAAACGG AGCCTGAGTGACAAGGAGCCTTCTTCAGGTCCTGAAGTGTCTGAAGAGCAGGCCTTACAGCTGGTCTGTA AGATCTTCCGTGTCTCCTGGAAGGACCGCGACAGAGATGTCATCTTCCTTTCTTCTCTCTCTGCGCAGTT TAAACAGAACCCAAAAGAAGTGTTCTCTGATTTTAAGGATCTGATTGGCCAGATTTTAATGGAAGTGCTG ATGATGTCCACTCAGACACGAGATGAAAATCCGTTTGCCAGTCTGACGGCCACATCCCAGCCCATCGCCA CGGCAGCTCGGTCACCAGACAGAAACCTCATGCTGAACACAGGCTCCAGTTCGGGGACAAGTCCCATGTT CTGCAGCTTGGGTTCCTTCAGCGCCAGCTCACTCTCCAGTTTGGGAGCCTCTGGTGGAGCAAGTACCTGG GATTCTTACAGTGACCATTTCACCATTGAAACCTGCAAAGAGACGGACATGCTGAACTACCTCATCGAGT GCTTTGACCGAGTTGGAATAGAGGAAAAAAAAGCACCAAAGATGTGCAGCCAGCCAGCAGTCAGCCAGCT TCTGAGCAACATCCGCTCGCAGTGCATCTCCCACACCGCGCTAGTGCTACAAGGGTCCCTCACACAGCCC AGGTCCTTGCAACAGCCATCCTTCCTCGTGCCGTACATGCTCTGCAGGAATCTCCCGTACGGCTTCATTC AAGAGCTGGTGAGGACCACTCACCAGGATGAAGAGGTGTTCAAGCAGATCTTTATCCCCATTTTACAAGG CCTGGCGCGTGCTGCCAAAGAGTGCTCCTTGGATAGCGACTACTTTAAGTACCCCCTCATGGCACTGGGT GAACTCTGTGAAACCAAGTTCGGGAAGACACACCCTATGTGCAATTTGGTCGCCTCCTTACCCCTGTGGT TGCCGAAGTCCTTAAGCCCTGGTTCTGGGCGGGAGCTGCAGAGACTCTCCTACTTAGGGGCCTTCTTTAG CTTCTCTGTCTTCGCAGAGGACGATGCAAAGGTGGTTGAGAAATACTTCTCAGGGCCTGCCATTACCCTG GAGAACACCCGTGTGGTCAGCCAGTCACTACAGCATTACCTGGAGCTGGGACGGCAAGAGCTTTTCAAGA TTCTGCATAGTATTTTGTTAAACGGCGAAACTCGTGAAGCAGCACTCAGTTACATGGCAGCCATTGTCAA TGCCAATATGAAGAAAGCGCAGATGCAGGCAGATGATCGGTTGGTGTCTACAGACGGGTTCATGCTGAAT CTCCTTTGGGTCCTGCAGCAGCTAAGTACAAAAATCAAGTTAGAAACGGTCGACCCCACTTACATATTCC ACCCGAGATGTCGGATCACTCTGCCAAACGATGAGACACGGATAAATGCAACGATGGAGGATGTGAACGA GTGGCTGACAGAGCTCTATGGAGATCAGCCTCCATTTTCTGAGCCAAAATTCCCTACAGAATGCTTCTTT CTCACCCTGCACGCTCACCATCTCTCTATCCTGCCTAGTTGTCGTCGCTACATTCGCCGGCTCCGAGCCA TCCGTGAGCTCAATAGAACCGTGGAAGATTTGAAAAATAATGAAAGCCAGTGGAAAGATTCCCCACTGGC CACCAGACACCGCGAAATGCTGAAGCGCTGTAAAACTCAGCTTAAGAAACTGGTACGGTGCAAGGCCTGT GCTGACGCTGGCTTACTTGACGAGAGCTTCCTAAGAAGATGTCTGAATTTTTATGGCCTTCTCATTCAGC TGATGCTGCGCGTCCTGGACCCTGCATATCCTGACATAACACTGCCTTTAAATTCAGAAGTCCCCAAGGT ATTTGCAGCATTGCCTGAGTTTTATGTAGAAGATGTTGCTGAATTTTTATTTTTTATTGTACAATACTCT CCTCAGGTGCTTTATGAGCCCTGCACTCAGGACATTGTGATGTTCCTCGTTGTGATGCTGTGCAACCAGA ACTACATCCGAAACCCTTACCTAGTGGCCAAGCTCGTGGAGGTCATGTTTATGACCAACCCCTCTGTTCA GCCGAGAACTCAGAAGTTTTTTGAGATGATTGAGAACCATCCTCTCTCTACCAAATTGCTGGTACCGTCC CTCATGAAGTTCTATACAGACGTCGAGCATACTGGAGCCACCAGCGAGTTCTATGACAAGTTCACAATTC GCTATCACATCAGCACTATTTTTAAAAGCCTTTGGCAAAACATAGCTCACCATGGCACCTTCATGGAGGA GTTCAATTCTGGAAAGCAGTTCGTTCGGTATATCAACATGCTGATAAATGACACGACCTTTTTACTGGAC GAAAGTCTGGAGTCTCTGAAGCGGATCCATGAAGTGCAAGAGGAGATGAAGAACCAAGAGCAGTGGGACC AGCTGCCCCGGGATCAGCAGCAGGCCCGGCAGTCTCAGCTTGCTCAGGACGAGCGTGTTTCCCGTTCCTA TCTCGCCCTGGCGACTGAAACCGTGGACATGTTCCATCTCCTCACCAAGCAAGTCCAGAAGCCGTTCCTG AGGCCAGAACTTGGTCCCCGGTTAGCAGCCATGCTGAACTTTAACCTGCAGCAGCTCTGCGGGCCCAAGT GCCGGGACCTCAAAGTGGAAAACCCAGAGAAGTACGGTTTTGAGCCAAAGAAGCTGTTGGACCAACTGAC GGATATCTACTTACAGCTGGACTGTGCTCGCTTTGCTAAAGCCATCGCTGATGACCAGCGATCCTACAGC AAGGAACTGTTCGAAGAAGTCATTTCAAAGATGCGGAAGGCAGGAATCAAATCCACCATCGCAATAGAAA AGTTTAAGCTTCTTGCAGAGAAAGTGGAGGAAATCGTGGCAAAGAATGCTCGGGCGGAAATAGACTACAG TGACGCCCCGGACGAGTTCAGAGACCCTCTGATGGACACCCTGATGACTGATCCCGTGAGACTGCCCTCT GGGACCATCATGGACCGTTCTATCATCCTGCGGCATCTGCTCAACTCCCCCACCGACCCCTTCAACCGCC AGATGCTGACTGAGAGCATGCTGGAGCCAGTGCCAGAGCTAAAGGAGCAGATTCAGGCCTGGATGAGAGA GAAGCAGAGCAGTGACCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217526 representing NM_001271198 Red=Cloning site Green=Tags(s) MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASVPGPSQSLGLNVHNTTPATSPIGAA GVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKR SLSDKEPSSGPEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVL MMSTQTRDENPFASLTATSQPIATAARSPDRNLMLNTGSSSGTSPMFCSLGSFSASSLSSLGASGGASTW DSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQP RSLQQPSFLVPYMLCRNLPYGFIQELVRTTHQDEEVFKQIFIPILQGLARAAKECSLDSDYFKYPLMALG ELCETKFGKTHPMCNLVASLPLWLPKSLSPGSGRELQRLSYLGAFFSFSVFAEDDAKVVEKYFSGPAITL ENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALSYMAAIVNANMKKAQMQADDRLVSTDGFMLN LLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRINATMEDVNEWLTELYGDQPPFSEPKFPTECFF LTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKAC ADAGLLDESFLRRCLNFYGLLIQLMLRVLDPAYPDITLPLNSEVPKVFAALPEFYVEDVAEFLFFIVQYS PQVLYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPSVQPRTQKFFEMIENHPLSTKLLVPS LMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLD ESLESLKRIHEVQEEMKNQEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHLLTKQVQKPFL RPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYS KELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPS GTIMDRSIILRHLLNSPTDPFNRQMLTESMLEPVPELKEQIQAWMREKQSSDH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271198
ORF Size	3519 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271198.1, NP_001258127.1
RefSeq Size	5627 bp
RefSeq ORF	3522 bp
Locus ID	298652
MW	133.3 kDa

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