Aff3 (NM_001191887) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Laf4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217552 representing NM_001191887 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACTCTAGGCCGTGTCTATGAACCAGATAGGAATGCGTTACGGAGGAAAGAACGGGAAAGGAGAA GTCAGGAAACGCAGCAGGACAGCGGCGGCTTTAACTCCGGTTACTCACTCTTCAGTGAACCCTACAAGAC TAACAAGGGGGATGAACTCTCCAACCGGATCCAGAATACATTAGGAAATTATGACGAGATGAAAGACTTT TTAACTGACCGATCCAACCAGAGTCATCTTGTTGGCGTCCCCAAGCCTGGTGTTCCCCAGACTCCTGTGA ACAAGATCGATGACCATTTTGTGGCAGAGTCAAGAGCCCAGCCCCAGCCCTCATCTGTCTGCAGCACTGC ATCTTCCACACCTGCAGCTGTCCCAGTGCAGCAGAGCAAGAGAGGCACCATGGGCTGGCAGAAGGCTGGG CACCCACCGTCTGACGGCCAACAGAGAGCAGCGCAACAGGGCTCTCTCAGGACCTTGCTTGGAGATGGTG TTGGCAGACAGCAGACAAGGGCCAAGCAAGTGTGCAATGTGGAGACGGGGCTTCAGACACAGGAGAGGCC GCCTGCAATGGCAGCCAAGCACAGTGGCGGTGGACACTGCGTTCAGAACTTTCCCCCATCCCTGGCTTCA AAACCCAGCCTGGGGCAGCAGAAACCAACCGCCTATGTGCGGCCAATGGATGGCCAGGACCAGGCTCCCG ACGAATCTCCTAAGCTTAAGTCATCCACGGAAACTGCTGTGCACTGCACGGCCTACAGAGGGGTCCCGGC CGGCAAACCAGAGTCAGCCAGAGCCAAGGGCAAGCTCGCTAAGTTCAGCATCCCCAAGCAAGGCGAGGAG AATAGATCTGGAGAAACCAACAGCTGTGTGGAAGAAATAATTCGGGAGATGACCTGGCTTCCTCCACTTT CCGCCATTCAAGCTCCTGCCAAGGTGGAACCAAGCAAGTTCCCATTCCCAAATAAGGACTCACAACTTGT ATCCTCTGGACACAGTAATCCAAAGAAAGCTGATGCGGAGCCAGAGAGTCCAGACAATGGCACGTCGAAC ACTTCAACTCTGGAAGACGACCTTAAGCTAAGCAGTGATGACGAAGAGGGTGAGCAGGCAGCCCAGAGAA CGGTTCTCCGCGCTCTAGCTGACAGCTCCGTGGTCCAGCAGACCAACTGTAGAGGTTCCGCGCCATCCAG CAAGGGCAGCAGCAGCAGCAGCGGCGGCAGCAGCTCCTCCAGCGACTCAGAAAGCACTTCCGGCTCCGAC TCCGAGACGGAGAGCAGCTCCAGCGAGAGCGAGAGTGAGGGCAGCAAGCCTCCGCACTGCTCCAGCCCCG AGGCCGAACCTGCGTCCTCGAACAAGTGGCAGTTGGATAAATGGCTGAACAAAGTTAATCCTCACAAGCC GCCAATTCTGATCCAAAATGAAAGCCATGGCCCGGAAAGGAATCAATACTACACGCCTGTGAAAGATGAA GGGCAAGACTGCGGGAAACTTCCTGAGATCTGCCAAGCCAGCCTGAGGGACAAGGAGCTCAAGACTGCCT GCAAGGAGGAGCAGCGGCCGAGGACCGCAAACAAGGTCCCTGGCAGCAAAAGTGTCAAGCAAAAGTCCCC GCCCGCTGCGGTGGCTGTGACCGCAGCTGCCCCGCCGCCCGCGGTGCCCAGCGCACCCACGGAGAGTGCA CCCGCGCCTACCCGCAGGTCAGCAGGCAAGAAGCCCACCAGGCGCACCGAGAGGACCTCAGCCGGGGACA GTGCCAACTGCCACCGACCAGAGGAGCCAGTGGCCCCAGACGCGCTGGGGGCGAGCGTAGTGGGACCCCT AGAGCCCCCCAAAACCAGGCCCTGTGGTAACAGCAGGACAGGCCACCGCAAGGAGCTGCGCTCCTCAGTG ACCTGTGAGAAGCGTCGCACGCGGGGGCTGAGCAGAATTGTCCCTAAATCCAAGGAGTTTATTGAAACAG AATCTTCGTCTTCCTCTTCCTCCTCGGACTCAGACCTGGAGTCAGAGCAAGAGGAGTACATCCTGTCCAA GGCTCCAAGCACCACCGGGAGTGAACAGAGGCTAAAGGAGGCCACCAGCAACAACAGCAGCAGCAGCAAC AGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCACCTCCCGAGCCTCTGTGGGCTCCA TCAACGCCAGGACCACCAGTGACATTGCCAAGGAGCTAGAGGAACAGTTCTACACGCTGGTCCCCTTTGG CAGGAATGAACTGCTTTCTCCACTAAAGGACAGTGATGAGGTCAGGTCTCTCTGGGTCAAAATTGACCTG ACACTCCTTTCCAGGATCCCAGAACACCTGTCCCAGGAGCCTGGGGTCTTGAGCGCTCCTGCAGCCAAGG ACACTGACAGTACCCCAGCCAGCCATGCATTGGACACACCTGCTGAAAAGACTTTGCCAAAATCCAAGAG GAAACGCAAGTGTGACAATGAAGACGACTACAGGGAGATAAAGAAGGCCCAGGGAAAGAAGGAGGGTGCC TCACGCCTGGCTGCCTCCACTAACAACACCTTCTCCGGCAGCCACTGCAACGTGAGCATCAACAGCTTGG CAATACCAATAAACAAAAATGAAAAGATGCTCCGGTCACCCAGCTCGCCACTCTCTGATGCATGTAAACA CAAATACTCCAGCGAGGACGTGACTTCTTCCAGCCGACCTCACGGCAACGGGTTGTTGACTTCTGTGTCT TCCAACAAAGAGCCTAAGGCCGAGAGCCAGCTGCAGTCGCATGCTGGGGACCTCACGAAGATCTCTCACA ACAGTTCCGAAAATGGCATCCTCCACAGCAAATCACGGCCCCAGACTGAGCCATGGTCTCCAGGTTCCAA CGGCCACAGGGACTGCAAGAGACAGAAACTGATCTTCGATGACATGCCCCGAAGTGCGGATTATTTCATG CGAGAAGCTAAGCGGATGAAACACAAAGCAGACGCCATGGTGGAGAAGTTTGGGAAGGCCCTCAACTATG CCGAAGCAGCCCTGTCCTTCATCGAGTGTGGAAACGCAATGGAACAAGGGCCGATGGAGTCCAAGTCTCC GTACACCATGTACTCGGAGACAGTGGAGCTCATCAGGTATGCTATGAGACTAAAAACCCACTCAGGCCCC AATGCCACACCGGAGGACAAACAGTTAGCTGCGCTTTGTTACCGATGCCTGGCTCTCCTATACTGGCGGA TGTTTCGACTCAAAAGGGACCATGCTGTCAAGTATTCAAAAGCACTCATCGACTATTTCAAGAATTCATC GAAAGCTGCCCAAGCCCCATCCCCATGGGGGTCTAGCGGCAAGAGCACTGGAAGCCCATCCCCCATGTCT CCCAACCCTTCTCCCGCCAGCTCCGTGGGGTCTCAGGGCAGCCTTTCCAGTTCCAGCGGCCTGTCCCCAT CGACCATTGTGAGCATCCCCCAGCGAATTCACCAGATGGCGGCCAATCACGTCAGCATCACCAACAGCAT CCTCCACAGCTACGACTACTGGGAAATGGCTGATAACCTGGCCAAGGAAAACAGAGAGTTCTTCAATGAC CTGGATTTGCTCATGGGACCTGTCACCCTGCACAGCAGCATGGAGCACCTGGTCCAGTACTCCCAGCAAG GCCTGCACTGGTTACGGAGCAGCACACACCTGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217552 representing NM_001191887 Red=Cloning site Green=Tags(s) MSTLGRVYEPDRNALRRKERERRSQETQQDSGGFNSGYSLFSEPYKTNKGDELSNRIQNTLGNYDEMKDF LTDRSNQSHLVGVPKPGVPQTPVNKIDDHFVAESRAQPQPSSVCSTASSTPAAVPVQQSKRGTMGWQKAG HPPSDGQQRAAQQGSLRTLLGDGVGRQQTRAKQVCNVETGLQTQERPPAMAAKHSGGGHCVQNFPPSLAS KPSLGQQKPTAYVRPMDGQDQAPDESPKLKSSTETAVHCTAYRGVPAGKPESARAKGKLAKFSIPKQGEE NRSGETNSCVEEIIREMTWLPPLSAIQAPAKVEPSKFPFPNKDSQLVSSGHSNPKKADAEPESPDNGTSN TSTLEDDLKLSSDDEEGEQAAQRTVLRALADSSVVQQTNCRGSAPSSKGSSSSSGGSSSSSDSESTSGSD SETESSSSESESEGSKPPHCSSPEAEPASSNKWQLDKWLNKVNPHKPPILIQNESHGPERNQYYTPVKDE GQDCGKLPEICQASLRDKELKTACKEEQRPRTANKVPGSKSVKQKSPPAAVAVTAAAPPPAVPSAPTESA PAPTRRSAGKKPTRRTERTSAGDSANCHRPEEPVAPDALGASVVGPLEPPKTRPCGNSRTGHRKELRSSV TCEKRRTRGLSRIVPKSKEFIETESSSSSSSSDSDLESEQEEYILSKAPSTTGSEQRLKEATSNNSSSSN SSSSSSSSSSSSSSSTSRASVGSINARTTSDIAKELEEQFYTLVPFGRNELLSPLKDSDEVRSLWVKIDL TLLSRIPEHLSQEPGVLSAPAAKDTDSTPASHALDTPAEKTLPKSKRKRKCDNEDDYREIKKAQGKKEGA SRLAASTNNTFSGSHCNVSINSLAIPINKNEKMLRSPSSPLSDACKHKYSSEDVTSSSRPHGNGLLTSVS SNKEPKAESQLQSHAGDLTKISHNSSENGILHSKSRPQTEPWSPGSNGHRDCKRQKLIFDDMPRSADYFM REAKRMKHKADAMVEKFGKALNYAEAALSFIECGNAMEQGPMESKSPYTMYSETVELIRYAMRLKTHSGP NATPEDKQLAALCYRCLALLYWRMFRLKRDHAVKYSKALIDYFKNSSKAAQAPSPWGSSGKSTGSPSPMS PNPSPASSVGSQGSLSSSSGLSPSTIVSIPQRIHQMAANHVSITNSILHSYDYWEMADNLAKENREFFND LDLLMGPVTLHSSMEHLVQYSQQGLHWLRSSTHLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191887
ORF Size	3672 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191887.2
RefSeq Size	3803 bp
RefSeq ORF	3675 bp
Locus ID	363220
MW	132.5 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.