Rbp3 (NM_001191832) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Irbp
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217556 representing NM_001191832 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAGAGAATGGGTCCTGGTTTTGTCCACACTGCTATGTGTTCTGGCTGGTCCCACGCACCTCTTCC AGCCAAGCCTGGTACTGGATATGGCCAAGGTCCTCCTGGATAACTACTGCTTCCCCGAGAACCTGATGGG AATGCAAGCGGCGATTGAGCAGGCTATGAAGAGTCATGAGATTCTGGGCATCTCGGACCCTCAGACGCTG GCCCATGTGCTGACAGTTGGAGTCCAGAGCTCTTTGAATGACCCACGTCTCTTCATCTCCTATGAGCCCA GTACCCTCGAGGCCCCCCAGCAAGCACCAGCACTCACCGACCTCACCCGAGAAGAGCTGCTGGCCCAGAT ACAGAGGAACATCCGCCATGAGGTTCTTGAGGATAACGTGGGCTACCTACGAGTGGATGATCTCCCTGGA CAGGAGGTACTGAGTGAGCTGGGGGAGTTCCTAGTGAGCCATGTGTGGAAGCAGCTCACGGGCACCTCCT CCTTGGTGCTAGATCTCCGTCACTGTGCTGGTGGTCATGTCTCTGGGATCCCTTATGTCATCTCCTACTT GCACCCTGGGAACACAGTCTTGCACGTGGATACCATCTACGATCGGCCCTCCAACACCACCACAGAGATC TGGACCTTGCCTAAGGTCCTGGGGGAGAGATACAGTGCAGACAAGGATGTGGTGGTCCTCACCAGTGGAC ACACTGGGGGAGTAGCCGAGGACATCGCTTACATCCTCAAGCAGATGCGCAGGGCCATCGTGGTGGGTGA GCGGACGGAAGGTGGCGCCCTGGACCTCCAGAAGCTGAGAATAGGTCAGTCTGACTTCTTCCTTACAGTG CCTGTGTCCAGGTCCCTGGGGCCCCTAGGTGGAGGCGGCCAAACATGGGAAGGCAGCGGGGTGCTGCCCT GTGTGGGGATACCTGCAGAGCAAGCCCTAGAAAAGGCCCTGGCCATCCTCACCCTGCGACGTGCCCTGCC AGGCGTTGTCCTCCGACTCCAGGAAGCCCTACAGGACTATTACACATTAGTGGACCGAGTGCCGGGCCTG CTGCACCACCTGGCCAGCATGGACTACTCGGCCGTGGTCTCCGAAGAGGACCTGGTGACAAAGCTCAATG CTGGTTTGCAGGCTGTGTCGGAGGATCCCAGGCTCCTGGTGAGAGCCACCGGACCCAGAGAAACCCCCTC AAGACCTGAGACTGGCCCTAACGAGCCACCAGCAGTCACCCCTGAGGTGCCTAAGGAAGAAGATGCCCGG AGGGCCCTAGTGGATTCCGTGTTTCAGGTGTCCGTGCTGCCGGGCAATGTGGGCTACCTGCGCTTTGACA GATTTGCAGATGCCTCTGTGCTGAAGACACTGGGCCCTTATGTGCTGCGGCAGGTGTGGGAGCCCCTGCA GGACACAGAGCACCTCATCATGGACCTGCGTCACAACCCTGGGGGTCCGTCCTCTGCCGTGCCTCTGGTT CTGTCCTACTTTCAGGGTCCGGAGGCTGGGCCCGTACGGCTCTTCACCACCTATGACCGTCGCACCAACA TCACCCAGGAGCATTTCAGCCACAAGGAGCTGCTAGGCCAACGATACGGCAACCGGCGTGGGGTGTACCT GCTTACTAGCCACAGGACTGCCACGGCTGCGGAAGAGTTTGCCTTCCTCATGCAGTCGCTGAGCTGGGCC ACGCTGGTAGGGGAAATCACCGCGGGCAGCCTGCTCCACACCTGCACGGTGCCACTGCTGGACTCGCCGG AGGGTGGCCTGACGCTCACGGTACCCGTGCTAACTTTTATCGACAACCACGGTGAGGCCTGGCTAGGGGG TGGGGTGGTGCCTGACGCCATCGTGCTGGCTGAGGAAGCCTTAGAGAGAGCTCAGGAGGTGCTGGATTTC CATCGCAGTCTGGGTGCCTTGGTAGAGGGCACCGGTCGCCTGCTGGAGGCTCACTATGCCCGGCCGGAGA TCGCAGAGCAGGCCAGAGATCTCCTGCAGTCTAAGCTGGCCCAAGGAGCCTACCGCACGGCTGTGGACTT GGAGTCGCTGGCCTCACAGCTCACTGCTGACCTTCAGGAGGTGTCTGAGGACCACCGTCTGTTGGTGTTC CACAGCCCCGGTGAGCTGGTGGCGGAGGAGGTCCCCCTGCCGCCCCCTGCCATCCCGTCCCCAGAGGAAC TCTCCTATCTCATAGAGGCCCTGTTCAAAACAGACGTGCTGCCAGGCCATCTGGGCTACTTGCGTTTTGA CGCCATGGCTGAGCTGGAGACAGTAAAGGCCATTGGGCCTCAGCTAGTGCGGCTGGTGTGGCAGAGGCTG GTGGACACGGCTGCACTGGTGGTTGACCTGCGCTACAATCCCGGCAGCTACTCCTCTGCCGTTCCGCTGC TCTGCTCCTACTTTTTTGAGGAAGAGCCCCGCCAGCACCTCTACTCCGTTTTTGACAGGGCGACATCGAG GGTCACAGAGATATGGACCCTGCCGCTGGTTGCCGGTCAACGCTACGGCTCCCATAAGGATCTCTACATC CTCATGAGCCATACCAGCGGCTCTGCAGCTGAGGCCTTTGCTCACACTATGCAGGATCTACAGCGGGCCA CCGTCATAGGAGAGCCCACAGCTGGAGGGGCACTCTCCGTGGGCATCTACCAGGTGGACAATAGCCCCTT ATATGCCTCCATGCCCACTCAGATGGCCCTGAGTGCCACCACTGGTGAGGCCTGGGACTTGGCTGGTGTG GAACCCGACATCACTGTGCCCATGAGCGAGGCCCTTTCCACAGCCCAGGACATAGTGGCCCTGCGTGCCA AGGTGCCCACAGTCCTGCAGACAGCCGGGAAACTAGTGGCCGATAACTATGCGTCCCCTGAACTGGGAGC CAAGATGGCTGCCAAGCTCAGCGGTCTGCAGAGCCGATATGCAAGGGTGACTTCAGAAGGGGCCTTGGCA GAGATGCTGGGGGCGGACCTGCAAATGCTCTCTGGGGACCCACACCTGAAGACGGCACACATCCCAGAAG ATGCCAAAGACAGAATTCCTGGAATTGTGCCCATGCAGATTCCCTCCCCAGAAGTCTTTGAGGACCTGAT CAAATTTTCCTTCCACACAAATGTATTTGAGGACAACATTGGCTACCTGAGGTTCGACATGTTTGGGGAT TGTGAGCTACTTATCCAGGTCTCAGAGCTGCTGGTAGAACACATCTGGAAGAAGATCGTGCACACTGATG CCCTCATCATCGACATGAGATTTAACATCGGCGGCCCCACCTCCTCCATCTCAGCCCTGTGCTCCTACTT CTTTGACGAGGCACCTCCCGTTCTGCTGGACAAGATCTACAACCGGCCCAACGACTCGATCAGCGAACTT TGGACACACTCGCAGCTCACAGGTGAACGATATGGCTCCAAGAAGAGTGTAGCCATCCTGACCAGCGGTG TGACAGCCGGCGCCGCCGAGGAATTTACTTACATCATGAAGAGGCTGGGCCGGGCCCTGGTCGTTGGTGA AGTGACAAGTGGAGGCTGCCAGCCACCACAGACCTACCACGTGGACGACACGCATCTCTATATCACCATC CCCACAGCTCGCTCTGTGGGCGCCACGGACGGCAGTTCCTGGGAAGGGGTGGGTGTGACACCTAATGTAG TTGTCTCTTCAGAATTGGCTCTTACCAGAGCCAAGGAGATACTCCAGCGGGCCCTGAGGGCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217556 representing NM_001191832 Red=Cloning site Green=Tags(s) MMREWVLVLSTLLCVLAGPTHLFQPSLVLDMAKVLLDNYCFPENLMGMQAAIEQAMKSHEILGISDPQTL AHVLTVGVQSSLNDPRLFISYEPSTLEAPQQAPALTDLTREELLAQIQRNIRHEVLEDNVGYLRVDDLPG QEVLSELGEFLVSHVWKQLTGTSSLVLDLRHCAGGHVSGIPYVISYLHPGNTVLHVDTIYDRPSNTTTEI WTLPKVLGERYSADKDVVVLTSGHTGGVAEDIAYILKQMRRAIVVGERTEGGALDLQKLRIGQSDFFLTV PVSRSLGPLGGGGQTWEGSGVLPCVGIPAEQALEKALAILTLRRALPGVVLRLQEALQDYYTLVDRVPGL LHHLASMDYSAVVSEEDLVTKLNAGLQAVSEDPRLLVRATGPRETPSRPETGPNEPPAVTPEVPKEEDAR RALVDSVFQVSVLPGNVGYLRFDRFADASVLKTLGPYVLRQVWEPLQDTEHLIMDLRHNPGGPSSAVPLV LSYFQGPEAGPVRLFTTYDRRTNITQEHFSHKELLGQRYGNRRGVYLLTSHRTATAAEEFAFLMQSLSWA TLVGEITAGSLLHTCTVPLLDSPEGGLTLTVPVLTFIDNHGEAWLGGGVVPDAIVLAEEALERAQEVLDF HRSLGALVEGTGRLLEAHYARPEIAEQARDLLQSKLAQGAYRTAVDLESLASQLTADLQEVSEDHRLLVF HSPGELVAEEVPLPPPAIPSPEELSYLIEALFKTDVLPGHLGYLRFDAMAELETVKAIGPQLVRLVWQRL VDTAALVVDLRYNPGSYSSAVPLLCSYFFEEEPRQHLYSVFDRATSRVTEIWTLPLVAGQRYGSHKDLYI LMSHTSGSAAEAFAHTMQDLQRATVIGEPTAGGALSVGIYQVDNSPLYASMPTQMALSATTGEAWDLAGV EPDITVPMSEALSTAQDIVALRAKVPTVLQTAGKLVADNYASPELGAKMAAKLSGLQSRYARVTSEGALA EMLGADLQMLSGDPHLKTAHIPEDAKDRIPGIVPMQIPSPEVFEDLIKFSFHTNVFEDNIGYLRFDMFGD CELLIQVSELLVEHIWKKIVHTDALIIDMRFNIGGPTSSISALCSYFFDEAPPVLLDKIYNRPNDSISEL WTHSQLTGERYGSKKSVAILTSGVTAGAAEEFTYIMKRLGRALVVGEVTSGGCQPPQTYHVDDTHLYITI PTARSVGATDGSSWEGVGVTPNVVVSSELALTRAKEILQRALRA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191832
ORF Size	3702 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191832.1, NP_001178761.1
RefSeq Size	3777 bp
RefSeq ORF	3705 bp
Locus ID	24711
MW	134.7 kDa
Gene Summary	may play a role in vision [RGD, Feb 2006]

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