Sik3 (NM_001271216) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217601 representing NM_001271216 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCGGCGGCGAGCGGAGCTGGCGGGGTTGCCGTGGCCGGAGCAGGGGGAGCCGGGCCGGCCG GTCGCCTGCTGCCTCCGCCCGCCGCGGGGCCCCCGGCAGCCCCCGCCGCAGTGCCCCCGGCCGCCTGCCC GCCGCGTCCCACAGCCCCGGCCTCCCGCGGGTCGATGGCCGCTCGCATCGGCTACTACGAGATCGACCGC ACCATCGGCAAGGGCAACTTCGCTGTGGTCAAGCGGGCCACGCACCTCGTCACCAAGGCCAAGGTTGCTA TTAAAATCATAGATAAGACCCAGTTGGATGAAGAGAACCTGAAGAAGATTTTCCGGGAGGTTCAGATAAT GAAGATGCTTTGCCACCCACATATCATCAGGCTCTACCAGGTCATGGAGACAGAGCGCATGATTTACCTG GTGACAGAATACGCTAGCGGAGGGGAGATATTTGACCACTTGGTGGCCCATGGAAGAATGGCGGAGAAGG AAGCTCGGCGGAAGTTCAAACAGATTGTCACAGCTGTATATTTTTGTCACTGTCGGAATATCGTTCATCG TGATTTAAAAGCTGAAAATTTGCTTCTGGACGCCAATCTGAATATCAAAATAGCAGACTTTGGCTTCAGC AACCTCTTCACTCCGGGTCAGCTGCTAAAGACGTGGTGTGGCAGTCCTCCCTATGCCGCACCAGAGCTCT TCGAAGGCAAGGAATATGATGGGCCCAAAGTGGACATATGGAGTCTTGGAGTTGTCCTCTATGTGCTTGT GTGTGGCGCCCTGCCGTTTGATGGGAGCACACTGCAGAATCTGCGGGCCCGTGTGCTGAGCGGCAAGTTC CGCATCCCGTTCTTCATGTCCACAGAGTGTGAGCACTTGATCCGCCACATGCTGGTGTTAGATCCAAATA AGCGTCTCTCAATGGAGCAGATCTGCAGGCACAGGTGGATGAAGCTCGGAGATGCAGACCCCAACTTTGA GAGGTTAATAGCAGAATGCCAGCAGCTGAAGGAGGAAAGGCAGGCAGAGCCCCTCAATGATGAGGTTCTC TTGGCCATGGAAGACATGGGACTAGACAAGGAACGCACACTTCAGTCATTAAGATCGGATGCCTATGACC ACTATAGTGCGATCTACAGCCTGCTGTGCGATCGACACAAGAAAAACAAAACTCTGCGTCCTGGAGCACT TCCCAGCACGCCCCAAGCCATGACTTTTCAGGCACCAGTCAATATCCAGGCGGAGCAGACAGGCACTGCT ATGAACATTAGCGTCCCCCAAGTTCAGCTGATCAACCCAGAGAACCAAATTATAGAGCCTGACGGGGCCG TGAACTTGGACAGCGACGAGGGTGAAGAGCCTTCTCCAGAAGCCTTGGTTCGCTATTTGTCAATGAGGAG GCACACGGTGGGAGTGGCTGACCCACGCACGGAAGTTATGGACGAGATGCAGAAGCTCCTGCCCGGCTTC CCTGGAGCGAACCCACAGACTCCATTCCTGCAGGTGGCCCCTAACATGAACTTCATGCACAACCTGCTGC CCATGCAGAGTCTGCAGCCAACTGGGCAGCTTGAGTACAAGGAGCAGTCTCTATTACAGCCGCCTACACT ACAGCTACTGAATGGAATGGGGCCCCTTGGCCGAAGAGCCTCAGACGGAGGCGCCAACATCCAACTACAT GCCCAGCAGCTGCTCAAGCGCCCACGGGGACCATCCCCACTTGTCACCATGACACCAGCAGTGCCAGCAG TTACCCCTGTGGATGAGGAGAGCTCGGATGGGGAGCCAGATCAGGAAGCTGTGCAGAGGTACTTGGCAAA TAGGTCCAAAAGGCACACACTGGCCATGACCAGCCCGACAGCTGAGATCCCACCGGACCTGCAACGGCAG CTAGGACAACAGTCTTTCCGTTCCCGGGTCTGGCCTCCTCACCTGGTACCTGACCAGCATCGCTCCACCT ACAAGGACTCCAACACCCTGCATCTCCCTACGGAGCGTTTCTCCCCCGTGCGCCGGTTCTCAGACGGGGC GGCAAGCATCCAGGCCTTCAAAGCTCACCTGGAAAGAATGGGCAACAGCAGCAGCATCAAACAGTTACAA CAGGAGTGTGAGCAGCTACAGAAGATGTACGGCGGGCAGGTTGACGAGAGAACCCTGGAGAAGACCCAGC AGCAGCATATGCTGTACCAGCAGGAGCAGCACCATCAGATCCTCCAGCAGCAAATTCAAGATTCTATTTG TCCTCCTCAGCCCTCCCCACCTCTTCAGGCTGCATGTGAAAATCAGCCAACCCTCCTCACCCACCAGCTG CAGAGGTTAAGGATTCAACCTTCAAGCCCACCCCCCAACCACCCCAGCAACCATCTCTTCAGGCAGCCAA GTAACAGTCCTCCCCCAGTCAGCAGTGCCATGATCTCTTCTCACGGTGCTACGTCTCCCTCCCAGTTTCA AGGCTTACCCTCCCATGGTGCAATCTTCCAGCAGCAACCTGAGAACTGTTCCCCACCTCCCAGTGTAGCA CTAACCTGCTTGGGCCTACAGCAGGCTAGCCAGTCACAGCCAGTGACTATCCAGCTACAGGAGCCAGTTG ACATGCTCAGCAACATGGCTGGGACAACTGCAGGTTCTGCAGGGCGGGGTATCCCCATCAGCCCCAGTGC CAGTCAGATTCAGATGCAGCACCGTACCAGCCTAATGGCTCCCTTCGGCTATGGGCACCGGCCCTTGTCC AAGCAGCTGAGTGCTGACAGCGCAGAGGCTCACAGCTTGAGCATGAATCGATTCTCCCCTGCCAACTACG ACCAGGCGCATTTACACCCCCATCTGTTCTCGGAGCAGTCCCGAGGGCCCCCCAGCAGCTACAGCCCTTC AACAGGAGTGGGGTTTCCTCCGGCTCAAGCCCTGAGAGTTCCTCCGCTTGACCAGTTCCCCACTTTCCCT CCCAGCGCCCATCAGCAGCCACACTATACCACATCAGCACTACAGCAGGCCCTGCTGTCCCCTACACCAC CAGACTATACCAGACACCAGCAGGTTCCCCACATCCTTCAAGGACTGCTCTCTCCCCGGCATTCACTCAC CGGCCACTCGGACATTCGGCTGCCTCCAGCAGAGTTTGCCCAGCTCATCAAAAGACAGCAGCAGCATCGA CAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAAGAATACCATGAGCTGTTCAGGCACATGAACCAAG GGGATGCTGTTAGCCTAGCTCCCGGCCTCGGGGGACAGAATATGACAGAGCACCAGGCTTTATCTTATCA AAATGCTGACTCATACCACCGCCACCACACCAGCCCCCAGCATCTCCTACAGATCAGAGCGCAAGATTGT ATCTCACAGGGTCCCTTGCCCACCCCCACCCGTGGGTATGCCCGTCAGTCACCACTAATGCATTCGGAGA GTATGGAAGAAGACTGCTTGTGTGAAGGGGTCAAGGAGGGCTTCGCGGACAAAAGCTCAAGCACACTGAC CAAAGGTTGCCATGACAGCCCTCTGCTCTTGTGTACCAGTGGGCCTGGGGACCCCGAGCCTTTGCTGGGA ACTGTGAGTCAGGCCCGGGAACTGGGAATCCATCCCTACGGACACCAGCCAACGGCCACCACATTCAGTA GAAATAAGGTGCCCAGCCGAGAGTCTGTCATAGGGAACTGCATGGAGAGAAGTTCTCCTGGACAAGCAAT GGAGCTGCCGGACCACAACGGCCTTGGGTATCCTGCACGGCCCTTAGTCAGTGAGCACCTCAGGTCCCGG ACCCTCCAGAGACACCACACCATCCAGAACAGCGACGATGCTTATGTACAGCTGGATACCTTGCCAGGAA TGAGCCTGGTGGCAGGAAAGGCACTGAGCTCTGCCCGGATGTCAGATGCAGTTCTTAGTCAGTCTTCACT CATGGGCAGCCAGCAGTTTCAGGATGAGGAAGATGAAGAATGTGGGGTGAGCCTGGGCCATGAGCACCCA GCCCTGGGTGACGGTAGCCAGCATTTAAACTCCCGCTATCCAGCTACGTGTGTTACAGACATCATGCTCA ACCACAAGCATCCAGAGGTCTCCTTCAGCATGGAGCAGGCTGGCGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217601 representing NM_001271216 Red=Cloning site Green=Tags(s) MAAAAASGAGGVAVAGAGGAGPAGRLLPPPAAGPPAAPAAVPPAACPPRPTAPASRGSMAARIGYYEIDR TIGKGNFAVVKRATHLVTKAKVAIKIIDKTQLDEENLKKIFREVQIMKMLCHPHIIRLYQVMETERMIYL VTEYASGGEIFDHLVAHGRMAEKEARRKFKQIVTAVYFCHCRNIVHRDLKAENLLLDANLNIKIADFGFS NLFTPGQLLKTWCGSPPYAAPELFEGKEYDGPKVDIWSLGVVLYVLVCGALPFDGSTLQNLRARVLSGKF RIPFFMSTECEHLIRHMLVLDPNKRLSMEQICRHRWMKLGDADPNFERLIAECQQLKEERQAEPLNDEVL LAMEDMGLDKERTLQSLRSDAYDHYSAIYSLLCDRHKKNKTLRPGALPSTPQAMTFQAPVNIQAEQTGTA MNISVPQVQLINPENQIIEPDGAVNLDSDEGEEPSPEALVRYLSMRRHTVGVADPRTEVMDEMQKLLPGF PGANPQTPFLQVAPNMNFMHNLLPMQSLQPTGQLEYKEQSLLQPPTLQLLNGMGPLGRRASDGGANIQLH AQQLLKRPRGPSPLVTMTPAVPAVTPVDEESSDGEPDQEAVQRYLANRSKRHTLAMTSPTAEIPPDLQRQ LGQQSFRSRVWPPHLVPDQHRSTYKDSNTLHLPTERFSPVRRFSDGAASIQAFKAHLERMGNSSSIKQLQ QECEQLQKMYGGQVDERTLEKTQQQHMLYQQEQHHQILQQQIQDSICPPQPSPPLQAACENQPTLLTHQL QRLRIQPSSPPPNHPSNHLFRQPSNSPPPVSSAMISSHGATSPSQFQGLPSHGAIFQQQPENCSPPPSVA LTCLGLQQASQSQPVTIQLQEPVDMLSNMAGTTAGSAGRGIPISPSASQIQMQHRTSLMAPFGYGHRPLS KQLSADSAEAHSLSMNRFSPANYDQAHLHPHLFSEQSRGPPSSYSPSTGVGFPPAQALRVPPLDQFPTFP PSAHQQPHYTTSALQQALLSPTPPDYTRHQQVPHILQGLLSPRHSLTGHSDIRLPPAEFAQLIKRQQQHR QQQQQQQQQQQQEYHELFRHMNQGDAVSLAPGLGGQNMTEHQALSYQNADSYHRHHTSPQHLLQIRAQDC ISQGPLPTPTRGYARQSPLMHSESMEEDCLCEGVKEGFADKSSSTLTKGCHDSPLLLCTSGPGDPEPLLG TVSQARELGIHPYGHQPTATTFSRNKVPSRESVIGNCMERSSPGQAMELPDHNGLGYPARPLVSEHLRSR TLQRHHTIQNSDDAYVQLDTLPGMSLVAGKALSSARMSDAVLSQSSLMGSQQFQDEEDEECGVSLGHEHP ALGDGSQHLNSRYPATCVTDIMLNHKHPEVSFSMEQAGV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271216
ORF Size	4107 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271216.2, NP_001258145.2
RefSeq Size	6248 bp
RefSeq ORF	4110 bp
Locus ID	684112
MW	150.9 kDa

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