Pla2r1 (NM_001100837) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Mrc2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217632 representing NM_001100837 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACAATGGTTAGCGATGCTCCAGCTGCTCTGGCTCCAGCAGCTGCTGCTGGGGGTTCACCAGGGCA GCGCCCAAGATTTGACCCACACCCAGGAACTGGCCCTCGAGTGGCGTGATAAAGGAATTTTCATTATCCA GAGCGAGAGCCTAAAGACTTGCATTCAGGCAGGTAAATCCGTACTGACCCTGGAGAACTGCACACAGCCA AACGAGTACATGCTGTGGAAATGGGTTTCCGACAACCACCTCTTTAATGTGGGAGCCAGCGGCTGCCTAG GCCTGAACATATCTGATCTGAAACGACCATTAAGTCTCTATGAATGTGATTCCACTCTCATTTCCTTGAG ATGGCTCTGTGACAGGAAGACGATCGTGGGCCCACTCCAGTACAAGGTCCAGGTGAACCACAGCACGGTG GTGGCAAAGAAAACACCCCATAAGTGGAGCGCCTACATGCCCGGTGGTGGGGACATTTGTGAGCATCTTC ACAGAGATATATATACCTTAAAAGGGAATGCCCATGGGATGCCTTGTGTGTTTCCTTTCCAGTTCAAAGG TCAGTGGCACCACGAGTGCATCCGAGAAGGGCATAAAGAGCACCTGCTGTGGTGTGCCACCACGAGTCGC TACGAAGAGGATGAGAAATGGGGATTCTGCCCAGATCCTACCTCTACAGAGATATTCTGTGATGCTATGT GGCAAAGGAACGACAATTCACACATTTGCTACCAATTCAACCTGCTCTCATCTCTCTCTTGGAATGAGGC ACATTCCTCATGCCAGATGCAAGGAGGTGCTTTGTTAAGTATTACGGATGAAAATGAAGAAGCTTTCATA AGGAAGGAGTTGAGCCGGGTAGCTAAAGAAGTGTGGACTGGTTTGAATCAGCTGGATGAGATGGCTGGCT GGCAGTGGTCTGATGGGACACCACTCAGCTACCTGAACTGGAGCCAAGAGGTCACTGCTGGGCCATTTGT GGAGTATCATTGTGGAACCCTGAAAGTTGCCTCAGCTACCTGGAGAAGCATGGACTGCGAGTCTACCTTG CCTTATATATGCAAACGGCATCTAAACCACACTGCCGAGGACATCCTGCAAAAGGACTCCTGGAAATACC ATACCACTCACTGTGACCCTGGCTGGACTCCCTTCAATCGTAAATGCTACAAACTTAAGAAAGAGAAAAG GACTTGGCAGGAGGCTCTGCACTCTTGCCAGTCTGATGACAGCATGTTGATGGATGTCACTTCCCTAGCT GAGGTGGAGTTGCTTGTAAACCTCCTTGGGGATGAAAATGCATCGGAAACATGGATTGGTTTGAGTAGCA ATAAAATTCCAGTTTCCTTCGCATGGTCCAGTGGCTCCTCTGTCATCTTCACTAACTGGTACCTTCTCGA GCCTCGAATTCTTCCAAACAGAAGACAACTGTGTGTCTCAGCAGATCAATCTGACGGACGCTGGAAAGTG AAAGGTTGTAAAGAAACACTCCCTTACATTTGTAAGAAAGCAGGACAAGTCCTTGAGGATGAGGACTCCG GATGTCAAGCGGGCTGGGAAAGACACGGCAAGTATTGTTACAAAATCGACACAGTCCTGAGAAGCTTTGA GCAGGCTTCCGGTGGTTATTACTGTCCTCCCGCCCTCCTAACCATCACCAGCCGATTTGAACAAGCTTTT ATTACCAGTTTGATCAGTAGTGTGGCAAGAAAGGACAGTTATTTTTGGATTGCTCTTCAAGACCAAAACA ATACAGGAGAATACACTTGGAAGACCATGGGACAGAGGGAGCCGGTGCAGTACACACACTGGAACGCACA TCAACCCAGCTACCATGGTGGCTGTGTTGTCCTGCGAGGAGGGAGTTCATTTGGTCGCTGGGAAGTGAAG AACTGTAGTGACTTTAAGGCAATGTCTCTTTGCAAGACACCCATCAAAGTCTGGGAGAAAACAGAGTTTG AAGGGAGGTGGCCCTTTCACCCCTGCTATATGGACTGGGAATCAGAGACTGGTCTAGCCAGTTGCTTCAA GGTCTTTCATAGCGAAAAGGTCCTGATGAAAAGATCATGGAGGGAAGCTGAAGCCTTCTGTGAGGAGTTT GGAGCTCACCTCGCAAGTTTTGCCCACATAGAGGAAGAAAACTTCGTGAATAAGCTTCTACACTCAAAAT TTAATTGGACACAAGAAAGGCAGTTCTGGATCGGATTTAATAAAAGGAACCCACTGAATGCCGGTTCTTG GGAGTGGTCTGACGGGTCTCCTGTTGTCTCTTCATTTTTAGACAATGCTTATTTTGAAGAAGATGCAAAA AACTGTGCTGTGTATAAGGCAAACAACACACTGTTGCCCTCGAACTGTGCTTCCAAACACGAGTGGATAT GCAAAATTCCAAGAGATGTGAAACCCAATTTTCCAGTCTGGTACCAGTACGATGCACCCTGGCTCTTCTA TCAGAATGCCGAGTACCTCTTCCACACCCATCCTGCCGAGTGGGCCACCTTTGAGTTTGTCTGCGGCTGG CTGCGAAGTGATTTCCTCACGATTTATTCTGCTCAGGAGCAAGAATTCATCCACAGCAAAATCAGAGCGC TATCAAAGTACGGGGCGAATTGGTGGATCGGACTTGAAGATAGAAGAGCTGGAGATCAAATTCACTGGAG TAATGGGTCACCCGTGATATTTCAGAACTGGGACAAAGGAAGGAAAGGAAGAATGGATAGCCAGAGAAAG CGATGCGTCTTCATGTCTGCTATAACAGGGCTCTGGGGCACTGAGAATTGCTCTGTTCCTATGCCCAGCA TCTGTAAGCGAGTGAAAATATGGGTCATCGAGAAGGAGAAGCCGCCAACACAGCATGGCACGTGCCCCAA AGGGTGGCTTTATTTTAACTATAAGTGCTTCCTGGTGACAATCCCCAAGGACCCACGGGAGCTGAAGACC TGGACAGGTGCTCAGGAATTCTGTGCTGCCAAGGGTGGGACGCTGGTCTCCATCGAAAGTGAACTGGAAC AAGCTTTCATTACTGTGAATCTTTTCGGCCAGACCACGAATGTATGGATAGGGTTACAAAGTGACAATCA TGAGAATTGGGTAAATGGGAAGCCTGTGGTGTATTCTAACTGGTCTCCATCTGATGTAATAAATATCCCC AATTATAACACCACTGAAGTTCAAAAGCGTGCTCCACTCTGTGCCCTGATGTCAAGTAATCCTAATTTCC ATTTTACTGGAAAATGGTATTTCGATGACTGTGGGAAAAAAGGCTATGGATTTGTGTGTGAAAAAATGCA AGATACCCTTGAAAACCATGTAAACGTGTCTGATACCTATGCAGTCCCCAGCACATTAGAATACGGAAAC AGGACTTATAAAATAATCCGTGCTAATATGACTTGGTACGCAGCAGGAAAGTCCTGCCGGATGCAGGGAG CAGAACTGGTCAGCATTCCAGACGTATTTCACCAGTCCTTCCTCACAGTTCTCCTCAGCAGGCTGGGGCA CGCCCACTGGATTGGGCTATCCACACCAGATAATGGTCTGAATTTTGACTGGTCAGACGGCACCAAATCC CCTTTCACCTATTGGAAAGATGAGGAGTCAGCCTCTCTTGGCGACTGCGTTTTTGCCGACACCGATGGTC ACTGGCATGGCACAGCCTGTGAGTCACATCTGCAAGGCGCCGTTTGTCGTGTACTCACTGAAACAAAGTC GTTTGAGCACCCAGTGCTGTGCTCAGAAACGTCAGTTCCCTGGATAAGATTTAAAGGTCATTGCTACAGT TTTTCTACAGTCCTGGACAGCAGAAGTTTTGAGGATGCTCGAGAATTCTGCAAAAGAGAAGGATCCAATC TTTTGGCAATCAAGGATGAGGCTGAAAATTTATTTCTCCTGGAAGAGCTTCTTGCCCTTGGTTCTTCTGT CCAGATGGTTTGGTTGAATGCTCAACTTGACAATGACAACAAAACCTTAAGGTGGTTTGACGGAGCAGCC ACAGAGCAGTCAAACTGGGGCGTACGGAAGCCAGACATGGACCACCTCAAGCCCCATCCGTGTGTTGTCC TGAGGATCCCTGAAGGAGTATGGCAGTCTACCCCATGCGAAGACAAAACGGGATTCATTTGTAAAGTGGA AGCAGGCATCCCAGCTGTAACAGCACATCCAGAGAAAGGACTAAGCCACAGCATCATGCCTGTTGCAGCA GCACTGGCGTTGACAATAGTCCTGGGCATTCTCGTCCTTTGCCTCTGGATTTACAAGCAGAATGGCGGTG CCATCTTGCCGAGACTCACGGGGTCGAGGGGCCCTTACTACCCTATGTCCAGCTTCAGCATGGCACACTT AGAAGAGAACATCCTCATTTCTGATCTTGAGAAGAATAACCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217632 representing NM_001100837 Red=Cloning site Green=Tags(s) MAQWLAMLQLLWLQQLLLGVHQGSAQDLTHTQELALEWRDKGIFIIQSESLKTCIQAGKSVLTLENCTQP NEYMLWKWVSDNHLFNVGASGCLGLNISDLKRPLSLYECDSTLISLRWLCDRKTIVGPLQYKVQVNHSTV VAKKTPHKWSAYMPGGGDICEHLHRDIYTLKGNAHGMPCVFPFQFKGQWHHECIREGHKEHLLWCATTSR YEEDEKWGFCPDPTSTEIFCDAMWQRNDNSHICYQFNLLSSLSWNEAHSSCQMQGGALLSITDENEEAFI RKELSRVAKEVWTGLNQLDEMAGWQWSDGTPLSYLNWSQEVTAGPFVEYHCGTLKVASATWRSMDCESTL PYICKRHLNHTAEDILQKDSWKYHTTHCDPGWTPFNRKCYKLKKEKRTWQEALHSCQSDDSMLMDVTSLA EVELLVNLLGDENASETWIGLSSNKIPVSFAWSSGSSVIFTNWYLLEPRILPNRRQLCVSADQSDGRWKV KGCKETLPYICKKAGQVLEDEDSGCQAGWERHGKYCYKIDTVLRSFEQASGGYYCPPALLTITSRFEQAF ITSLISSVARKDSYFWIALQDQNNTGEYTWKTMGQREPVQYTHWNAHQPSYHGGCVVLRGGSSFGRWEVK NCSDFKAMSLCKTPIKVWEKTEFEGRWPFHPCYMDWESETGLASCFKVFHSEKVLMKRSWREAEAFCEEF GAHLASFAHIEEENFVNKLLHSKFNWTQERQFWIGFNKRNPLNAGSWEWSDGSPVVSSFLDNAYFEEDAK NCAVYKANNTLLPSNCASKHEWICKIPRDVKPNFPVWYQYDAPWLFYQNAEYLFHTHPAEWATFEFVCGW LRSDFLTIYSAQEQEFIHSKIRALSKYGANWWIGLEDRRAGDQIHWSNGSPVIFQNWDKGRKGRMDSQRK RCVFMSAITGLWGTENCSVPMPSICKRVKIWVIEKEKPPTQHGTCPKGWLYFNYKCFLVTIPKDPRELKT WTGAQEFCAAKGGTLVSIESELEQAFITVNLFGQTTNVWIGLQSDNHENWVNGKPVVYSNWSPSDVINIP NYNTTEVQKRAPLCALMSSNPNFHFTGKWYFDDCGKKGYGFVCEKMQDTLENHVNVSDTYAVPSTLEYGN RTYKIIRANMTWYAAGKSCRMQGAELVSIPDVFHQSFLTVLLSRLGHAHWIGLSTPDNGLNFDWSDGTKS PFTYWKDEESASLGDCVFADTDGHWHGTACESHLQGAVCRVLTETKSFEHPVLCSETSVPWIRFKGHCYS FSTVLDSRSFEDAREFCKREGSNLLAIKDEAENLFLLEELLALGSSVQMVWLNAQLDNDNKTLRWFDGAA TEQSNWGVRKPDMDHLKPHPCVVLRIPEGVWQSTPCEDKTGFICKVEAGIPAVTAHPEKGLSHSIMPVAA ALALTIVLGILVLCLWIYKQNGGAILPRLTGSRGPYYPMSSFSMAHLEENILISDLEKNNQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001100837
ORF Size	4383 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100837.1, NP_001094307.1
RefSeq Size	5599 bp
RefSeq ORF	4386 bp
Locus ID	295631
MW	167 kDa

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