Pola1 (NM_053479) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217636 representing NM_053479 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCTGTTCACGGAGACGACTGCAAACTGGAAACAAGCGCTGTGTCAGATTCAGGGAGTTTTGTAG CTTCTCGAGCACGTCGAGAAAAAAAATCAAAGAAAGGACGCCAAGAAGCTTTAGAGCGACTGAAAAAGGC TAAAGCTGGTGAGAAGTACAAGTATGAAGTTGAGGACCTCACAAGTGTTTATGAAGAAGTTGATGAAGAG CAGTATTCAAAACTGGTTCAGGCACGTCAGGATGATGACTGGATCGTGGATGACGACGGTATAGGCTATG TGGAAGACGGTAGAGAGATTTTTGATGACGACCTTGAAGATGATGCACTTGACACCTGTGGGGAAGGAAG TGATGGCAAAGCACACAGAAAGGACAGGAAGGATGTAAAGAAACCTTCAGTGACAAAACCAAACAATATC AAGGCAATGTTCATTGCTAGTGCTGGGAAGAAAACCACAGATAAAACAGTTGACTTGTCCAAGGATGATC TGCTAGGTGACATTCTACAGGATCTGAACACTGAGACACCTCAAATAGCTCCACCGCCAGTATTGATACC AAAGAAAAAAAGATCTACTGGAGCTTCACCGAATCCTTTCTCTGTGCACACCGCCACGGCAGTTCCTTCA GGAAAAATTGCTTCCCCTGTCTCCAGAAAGGAGCCTCCATTAACTCCTGTTCCTCTTAAACGTGCTGAAT TTGCTGGCGATCTGGCTCAGCCAGAGTGTCCAGAAGATGAGCAGGAGTCAGGGGTTATAGAGTTTGAAGA TGGCGACTTTGATGAGCCCATGGACACAGAGGAAGTAGATGAGGAGGAGCCTGTGACTGCCAAGATTTGG GACCAAGAGAGTGAGCCAGTGGAAGGGGTGAAGCATGAGGCAGATCCTGAGACGGGAACCACATCTTTCT TAGACAGTTTTCTCCCAGATGTTTCTTGTTGGGACATTGATCAGAAGGACGAAAACAGTTTCTTACTTCA AGAAGTTCAAGTGGACTCCAATCATCTCCCGTTGGTAAAAGGGGCAGATGATGAACAAGTCTTTCAGTTT TATTGGCTGGATGCTTATGAAGATCCATACAACCAACCAGGTGTGGTTTTTCTGTTTGGCAAAGTATGGG TCGAATCAGCCAAAACCCATGTGAGCTGTTGTGTTATGGTGAAAAATATTGAGCGAACACTCTACTTCCT TCCCCGTGAAATGAAAATTGACCTAAACACAGGGAAAGAAACAGCAACTCCAATTACAATGAAGGATGTT TATGAAGAATTTGATTCAAAAATTTCAGCAAAATATAAAATAATGAAGTTCAAGTCCAAGATAGTGGAAA AGAACTATGCTTTTGAGATACCTGATGTTCCAGAAAAATCTGAGTACTTGGAAGTTAGATACTCGGCAGA AGTGCCACAGCTTCCACAGAATTTAAAAGGAGAAACATTTTCTCATGTATTTGGAACTAACACGTCTAGC TTGGAACTGTTCTTGATGAATAGAAAGATCAAAGGACCCTGTTGGCTTGAAGTAAAAAATCCACAACTCT TGAATCAACCAATCAGTTGGTGTAAATTTGAGGCAATGGCTTTGAAACCAGACTTGGTGAATGTAATTAA GGATGTCAGTCCTCCTCCACTTGTGGTGATGTCTTTCAGCATGAAGACAATGCAAAATGTACAGAACCAT CAACACGAGATCATTGCTATGGCAGCGCTGGTTCATCACAATTTCCCATTAGATAAAGCACCTCCAAAAC CTCCCTTTCAGACGCATTTCTGTGTTGTGTCTAAACCAAAGGACTGTATTTTCCCATGCGCTTTCAAAGA AGTCATTAAGAAAAAGAATATGGAGGTTGAGGTTGCTGCAACAGAAAGAACTTTGCTAGGATTTTTCCTT GCAAAAGTTCACAAACTTGACCCTGATATTCTCGTGGGTCATAATATTTGTGGTTTTGAACTAGAAGTGC TACTGCAAAGAATTAATGAGTGCAAGGTTCCTTTCTGGTCCAAGATAGGTCGGCTGAGGAGATCCAACAT GCCAAAACTTGGGAGCCGGAGTGGATTTGGTGAAAGAAATGCTACCTGTGGTCGAATGATATGTGATGTG GAAATTTCAGTAAAAGAATTGATTCATTGTAAAAGTTACCATTTGTCTGAACTTGTTCAGCAGATTCTGA AAACTGAGAGGATTGTAATTCCAACAGAAAATATACGAAACATGTATAGTGAACCTTCTCATCTGTTGTA CCTGTTGGAACACATCTGGAAAGATGCCAGATTCATTTTGCAGATCATGTGTGAGCTAAATGTTCTTCCA TTAGCATTGCAGATCACTAACATCGCTGGGAACATTATGTCTAGGACACTGATGGGTGGACGATCTGAGC GAAACGAGTTTTTGTTGCTTCATGCATTTTATGAAAACAACTATATTGTGCCTGACAAGCAGATTTTCAG AAAACCTCAGCAAAAACCGGGAGATGAAGATGAAGAAATTGATGGGGATACCAATAAATATAAGAAAGGA CGCAAGAAAGCAGCTTATGCTGGAGGCTTGGTTTTGGACCCCAAAGTTGGTTTTTATGACAAGTTCATTT TGCTCCTGGATTTCAACAGTTTATATCCTTCCATCATTCAGGAATTTAACATTTGTTTTACAACAGTGCA AAGAGTTGCTTCAGAGACACTGAAAGCTACAGAGGATGAGGAACAAGAACAGATTCCAGAGTTGCCAGAT CCAAACTTAGATATGGGCATTTTGCCCAGAGAGATCCGGAAACTGGTAGAGCGGAGAAAACAAGTGAAAC AGCTAATGAAACAGCAAGATTTAAATCCAGACCTTGTTCTTCAGTATGATATCCGACAGAAGGCTTTGAA GCTCACAGCGAACAGTATGTATGGTTGCCTGGGATTTTCGTACAGCAGATTTTATGCTAAACCGCTGGCT GCCTTGGTGACATACAAAGGAAGGGAGATTTTGATGCACACGAAAGAGATGGTACAGAAGATGAATCTTG AAGTTATTTATGGAGACACAGATTCAATTATGATAAATACCAATAGCACCAACCTGGAAGAAGTCTTTAA GTTGGGAAACAAGGTAAAAAATGAAGTGAATAAATTGTACAAACTGCTTGAAATAGACATTGATGGTGTT TTCAAGTCTCTGCTATTGCTGAAGAAAAAGAAATATGCTGCTCTAGTTGTCGAACCAACATCCGATGGGA ATTACATCACCAAGCAGGAACTGAAAGGATTGGATATAGTTAGAAGGGACTGGTGTGATCTTGCCAAGGA CACCGGAAACTTTGTCATTGGCCAGATTCTTTCTGATCAAAGTCGGGACACCATTGTGGAAAACATTCAG AAGAGGTTAATAGAAATTGGAGAAAATGTGCTAAACGGCAGTGTCCCAGTGAGCCAGTTTGAAATTAACA AGGCATTGACAAAGGATCCCCAGGATTACCCTGATAAAAAAAGTCTACCTCATGTACATGTTGCACTGTG GATAAATTCTCAAGGAGGCAGGAAGGTGAAAGCTGGCGATACAGTGTCATATGTTATCTGTCAGGATGGG TCAAACCTCCCTGCAACTCAGAGGGCCTATGCACCCGAGCAATTGCAGAAACAGGACAATTTAGCCATCG ACACCCAGTACTACTTGGCCCAGCAGATCCACCCTGTTGTGGCTCGAATCTGTGAGCCAATAGATGGAAT TGATGCTGTACTCATTGCATTATGGCTAGGACTTGACTCCACTCAATTTAGAGTTCATCAGTATCACAAA GATGAAGAGAATGATGCTCTCCTTGGTGGTCCAGCACAGCTAACTGATGAAGAGAAATACAAGGATTGTG AAAAATTCAAATGCCTTTGTCCTTCATGTGGAACTGAAAATATTTATGATAATGTCTTTGAAGGCTCGGG AATGGATATGGAGCCCAGCTTGAATCGTTGCAGTAATATTGATTGTAAGGCTTCACCAGCGACCTTTATG GTACAGTTGAGCAACAAATTGATCATGGACATTAGACGTTGCATTAAAAAGTACTATGATGGCTGGTTGA TATGTGAGGAGCCAACTTGTCGCAATCGCATTCGTCGTCTTCCCCTTCATTTCTCCCGAAATGGGCCTCT GTGTCCAGCCTGCATGAAAGCTGTGCTCAGACCAGAGTATTCTGACAAGTCCCTGTACACCCAGCTGTGC TTTTACCGGTACATTTTTGATGCGGACTGTGCACTGGAAAAACTTCCTGAACACGAGAAAGATAAATTGA AGAAGCAGTTTTTCACTCCCAGAGTTCTTCAGGACTACAGAAAAGTCAAGAACATAGCAGAACACTTCTT GTCCTGGAGTGGCTACTCTGAAGTAAACCTCAGCAAGCTCTTCGCCAATTATGCTGGGAAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217636 representing NM_053479 Red=Cloning site Green=Tags(s) MAPVHGDDCKLETSAVSDSGSFVASRARREKKSKKGRQEALERLKKAKAGEKYKYEVEDLTSVYEEVDEE QYSKLVQARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDTCGEGSDGKAHRKDRKDVKKPSVTKPNNI KAMFIASAGKKTTDKTVDLSKDDLLGDILQDLNTETPQIAPPPVLIPKKKRSTGASPNPFSVHTATAVPS GKIASPVSRKEPPLTPVPLKRAEFAGDLAQPECPEDEQESGVIEFEDGDFDEPMDTEEVDEEEPVTAKIW DQESEPVEGVKHEADPETGTTSFLDSFLPDVSCWDIDQKDENSFLLQEVQVDSNHLPLVKGADDEQVFQF YWLDAYEDPYNQPGVVFLFGKVWVESAKTHVSCCVMVKNIERTLYFLPREMKIDLNTGKETATPITMKDV YEEFDSKISAKYKIMKFKSKIVEKNYAFEIPDVPEKSEYLEVRYSAEVPQLPQNLKGETFSHVFGTNTSS LELFLMNRKIKGPCWLEVKNPQLLNQPISWCKFEAMALKPDLVNVIKDVSPPPLVVMSFSMKTMQNVQNH QHEIIAMAALVHHNFPLDKAPPKPPFQTHFCVVSKPKDCIFPCAFKEVIKKKNMEVEVAATERTLLGFFL AKVHKLDPDILVGHNICGFELEVLLQRINECKVPFWSKIGRLRRSNMPKLGSRSGFGERNATCGRMICDV EISVKELIHCKSYHLSELVQQILKTERIVIPTENIRNMYSEPSHLLYLLEHIWKDARFILQIMCELNVLP LALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQIFRKPQQKPGDEDEEIDGDTNKYKKG RKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASETLKATEDEEQEQIPELPD PNLDMGILPREIRKLVERRKQVKQLMKQQDLNPDLVLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLA ALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKNEVNKLYKLLEIDIDGV FKSLLLLKKKKYAALVVEPTSDGNYITKQELKGLDIVRRDWCDLAKDTGNFVIGQILSDQSRDTIVENIQ KRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDG SNLPATQRAYAPEQLQKQDNLAIDTQYYLAQQIHPVVARICEPIDGIDAVLIALWLGLDSTQFRVHQYHK DEENDALLGGPAQLTDEEKYKDCEKFKCLCPSCGTENIYDNVFEGSGMDMEPSLNRCSNIDCKASPATFM VQLSNKLIMDIRRCIKKYYDGWLICEEPTCRNRIRRLPLHFSRNGPLCPACMKAVLRPEYSDKSLYTQLC FYRYIFDADCALEKLPEHEKDKLKKQFFTPRVLQDYRKVKNIAEHFLSWSGYSEVNLSKLFANYAGKN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053479
ORF Size	4404 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053479.1, NP_445931.1
RefSeq Size	5321 bp
RefSeq ORF	4407 bp
Locus ID	85241
UniProt ID	O89042
MW	167.2 kDa
Gene Summary	exhibits DNA polymerase alpha activity; may mediate DNA replication process [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.