Robo2 (NM_032106) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217649 representing NM_032106 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTCCTCTGATGTTTACACTACTATTGCTCTTTGGATTTCTCCGCATTCGGACTGACGGATNTCGTC TTCGTCAAGAAGACTTTCCCCCCAAGATNGTAGAACAACCTTCTGAAGTCATCGTCTCCAAAGGAAAACC CAACACTCCGAACTGGAAGCAAAAGGGCCGGCCATTCCCAACAATTGGAAAGGTACAAAGGATGGTTAAA CCGGGTTGGGACAAGACCAAGGATGATTCCAAGGTCACACAAGGATGCCTTCTGCCCAGCGGATCCTTAT TCTTTTTGCGAATTGTTCACGGACGCAGAAGTAAACCAGATGAAGGAACTTACGTTTGTGTTGCAAGGAA CTATCTTGGTGAAGCAGTGAGTCGCAATGCATCTCTGGAAGTAGCATTATTGCGAGATGACTTCCGGCAA AACCCCACAGATGTGGTAGTCGCAGCAGGAGAGCCTGCAATCTTGGAGTGCCAGCCACCACGGGGACACC CAGAACCAACCATCTACTGGAAAAAGGACAAAGTCCGAATTGATGAGAAGGAAGAGAGAATAAGTATCCG TGGTGGGAAGCTGATGATCTCCAATACTAGGAAAAGCGATGCTGGCATGTATACCTGTGTGGGAACCAAC ATGGTGGGAGAAAGGGACAGCGACCCTGCAGAGCTCACTGTCTTTGAACGACCCACGTTTCTCAGGAGGC CAATCAACCAGGTGGTGCTGGAGGACGAGCCTGCAGAATTCCGCTGTCAGGTCCAAGGAGATCCCCAGCC AACGGTGAGGTGGAAAAAGGATGACGCAGACTTACCAAGAGGAAGGTATGATATCAAGGACGACTACACC CTGAGAATCAAAAAGGCCATAAGTGCAGATGAAGGCACCTATGTGTGCATTGCTGAGAACCGGGTGGGAA AAGTGGAAGCTTCTGCCACCCTCACTGTCCGAGCTCCTCCACAGTTTGTGGTTAGGCCAAGAGATCAGAT CGTTGCTCAGGGCCGGACAGTGACATTCCCCTGTGAAACTAAAGGAAACCCACAGCCAGCTGTCTTCTGG CAGAAAGAAGGCAGCCAGAACCTACTTTTCCCGAATCAACCTCAGCAGCCCAATAGCCGCTGTTCAGTGT CGCCCACGGGGGACCTCACCATCACCAACATTCAGCGTTCAGATGCGGGTTACTACATCTGCCAGGCCCT AACTGTGGCAGGAAGCATTTTAGCTAAAGCACAGCTGGAAGTTACTGACGTTTTGACAGATAGACCTCCA CCCATAATCTTGCAAGGACCAATAAACCAAACACTTGCGGTAGATGGCACAGCATTACTGAAATGTAAAG CCACTGGACCTCTTCCTGTAATTAGCTGGCTAAAGGAGGGCTTTACTTTTCTGGGTAGAGATCCAAGAGC TACGATCCAAGACCAAGGAACACTGCAGATTAAGAATTTACGGATTTCTGATACTGGCACTTATACATGT GTGGCTACAAGTTCGAGTGGGGAGACTTCCTGGAGTGCAGTGCTGGATGTGACAGAGTCTGGAGCAACAA TCAGTAAAAATTATGATACAAATGACCTGCCCGGGCCACCATCCAAACCTCAGGTCACTGATGTTACGAA GAACAGTGTCACCCTATCCTGGCAGACAGGTACCCCTGGCGTTCTTCCAGCAAGCGCATATATCATTGAG GCTTTCAGCCAATCGGTGAGCAACAGCTGGCAGACAGTGGCAAACCATGTTAAGACGACTCTCTATACAG TCAGAGGACTAAGGCCCAACACAATCTATCTGTTCATGGTCAGAGCGATCAACCCCCAAGGTCTCAGTGA TCCAAGCCCCATGTCGGATCCTGTACGCACACAAGATATCAGTCCCCCAGCACAAGGCGTGGACCACAGA CAAGTGCAGAAGGAGTTAGGAGATGTCACTGTTCGTCTCCACAATCCAGTTGTCCTGACACCTACAACTG TTCAAGTCACATGGACGGTGGACCGACAACCCCAGTTTATTCAGGGCTACAGAGTGATGTACCGACAGAC TTCTGGTCTACAAGCTTCAACTGTGTGGCAGAATCTAGATGCCAAAGTCCCGACTGAGAGGAGTGCTGTC CTTGTGAATCTGAAAAAGGGGGTGACTTATGAAATTAAAGTCAGGCCATATTTCAATGAGTTCCAAGGAA TGGACAGTGAATCAAAAACAATCCGTACTACTGAGGAAGCTCCAAGTGCGCCTCCCCAGTCTGTCACGGT GCTGACCGTTGGAAGCCATAACAGCACAAGCATTAGTGTTTCCTGGGATCCTCCACCAGCCGACCACCAG AATGGAATCATACAGGAATACAAGATCTGGTGTCTGGGAAATGAAACGCGATTCCATATCAATAAAACTG TGGATGCAACCATTCGCTCTGTAGTAATAGGTGGCTTGTTCCCTGGAATTCAGTACCGGGTGGAAGTGGC AGCTAGTACAAGTGCAGGGGTTGGAGTAAAAAGTGAACCACAGCCAATAATCATTGGGGGACGTAACGAA GTTGTCATTACTGAAAACAATAACAGCATAACTGAGCAAATCACTGATGTCGTGAAGCAACCAGCATTTA TAGCTGGCATCGGTGGTGCCTGCTGGGTAATTTTGATGGGTTTTAGCATCTGGTTATACTGGAGAAGAAA GAAGAGAAAGGGACTTAGCAATTATGCTGTAACATTTCAAAGAGGAGATGGAGGACTAATGAGCAATGGG AGCCGTCCAGGTCTTCTAAATACTGGCGACCCCAATTATCCATGGCTTGCTGACTCATGGCCAGCCACGA GTTTGCCAGTCAACAACAGCAATAGTGGCCCAAATGAAATCGGAAATTTTGGGCGTGGAGATGTGCTGCC TCCAGTCCCAGGCCAAGGGGATAAAACAGCAACAATGCTCTCAGATGGAGCTATTTATAGTAGCATTGAC TTCACTACCAAGACCACTTACAACAGTTCCAGCCAAATAACGCAGGCCACCCCATACGCCACTACCCAGA TCCTGCATTCCAACAGCATCCATGAACTGGCGGTTGATCTTCCTGATCCACAGTGGAAAAGCTCAGTTCA ACAGAAATCAGACCTCATGGGATTTGCTTATTCACTACCTGATCAGAACAAGGGGAACAATGCCTTACTT TACATCCCTGACTACCGATTGGCTGAGGGATTGTCTAATAGAATGCCACACAACCAGTCACAGGATTTCA GCACCACCAGCTCTCACAACAGCTCAGAAAGGAGTGGCAGTCTCTCAGGTGGGAAAGGTGGAAAAAAGAA GAAAACTAAAAATTCTTCAAAGGCGCAAAAAAACAACGGATCCACTTGGGCTAATGTCCCTCTACCTCCT CCTCCTGTCCAGCCCCTTCCGGGTACAGAGCTCGGACACTATCCTGCGGAACAGGAAAATGGCTATGACA GTGATAGCTGGTGCCCACCATTACCAGTGCAAACCTATTTGCATCAGGGAATGGAAGACGAGTTGGAAGA GGATGAAGATCGCGTCCCAACACCTCCTGTTCGCGGTGTGGCCTCTTCACCCGCTATCTCCTTTGGGCAG CAGTCCACTGCCACTCTTACACCATCCCCACGGGAGGAGATGCAACCCATGCTGCAAGCTCACTTGGATG AGTTAACCAGGGCCTATCAGTTTGATATAGCAAAACAAACATGGCACATTCAAAGCAATACTCCACCTCC ACAACCCCCAGTTCCACCATTAGGTTACGCGTCCGGAGCCTTGATTTCTGACTTGGAGACAGATGTTCCA GATGAGGATGCTGATGATGAAGAGGAACCATTAGAAATTCCCAGGCCCCTCAGAGCACTAGACCAGACTC CTGGTTCCAGTATGGACAATCTAGACAGCTCTGTAACAGGAAAAGCCTTTACTTCCTCTCAAAGGCAGCG GCCCACCAGCCCATTTTCTACAGACAGTAACACCAGTGCTGCCCAGAATCAAAGCCAGAGGCCTCGGCCC ACCAAAAAACACAAGGGAGGGCGGATGGACCCACAGCCAGTGTTACCTCATCGGAGGGAAGGGATGCCAG ATGATCTTCCCCCACCACCAGATCCCCCACCAGGTCAGGGTTTAAGGCAGCAGATAGGCCTGAGCCAACA CTCTGGTAACGTGGAAAGCTCAACAGAGAGGAAAGGAAGCTCTCTAGAGAGACAACAAGCAGCCAACTTA GAAGACACAAAGAGCTCATTGGATTGTCCGGCTAAAACTACTGTAGAGTGGCAGCATCAAACCCAGGACT GGATAAACTCTACAGAGCGCCAAGAAGAAACACGGAAAGCCCCACACAAACCAGGGGTTGGATCAGAGGA GTCCCTGGTGCCCTACAGCAAGCCCAGCTTCCCATCTCCAGGCGGGCACAGCTCATCCGGAACAGCCTCC TCTAAAGGGTCCACTGGTCCTCGGAAGGCTGAGATTCTGAGGGGCAGCCACCAGCGGAATGCCAATGACC TTCTCGACATTGGATATGTGGGCTCAAATAGTCAAGGGCAGTTTACAGGTGAATTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217649 representing NM_032106 Red=Cloning site Green=Tags(s) MTPLMFTLLLLFGFLRIRTDGXRLRQEDFPPKXVEQPSEVIVSKGKPNTPNWKQKGRPFPTIGKVQRMVK PGWDKTKDDSKVTQGCLLPSGSLFFLRIVHGRRSKPDEGTYVCVARNYLGEAVSRNASLEVALLRDDFRQ NPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDEKEERISIRGGKLMISNTRKSDAGMYTCVGTN MVGERDSDPAELTVFERPTFLRRPINQVVLEDEPAEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYT LRIKKAISADEGTYVCIAENRVGKVEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFW QKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPP PIILQGPINQTLAVDGTALLKCKATGPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTC VATSSSGETSWSAVLDVTESGATISKNYDTNDLPGPPSKPQVTDVTKNSVTLSWQTGTPGVLPASAYIIE AFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHR QVQKELGDVTVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAV LVNLKKGVTYEIKVRPYFNEFQGMDSESKTIRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQ NGIIQEYKIWCLGNETRFHINKTVDATIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNE VVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNG SRPGLLNTGDPNYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSID FTTKTTYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSVQQKSDLMGFAYSLPDQNKGNNALL YIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKTKNSSKAQKNNGSTWANVPLPP PPVQPLPGTELGHYPAEQENGYDSDSWCPPLPVQTYLHQGMEDELEEDEDRVPTPPVRGVASSPAISFGQ QSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNTPPPQPPVPPLGYASGALISDLETDVP DEDADDEEEPLEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRQRPTSPFSTDSNTSAAQNQSQRPRP TKKHKGGRMDPQPVLPHRREGMPDDLPPPPDPPPGQGLRQQIGLSQHSGNVESSTERKGSSLERQQAANL EDTKSSLDCPAKTTVEWQHQTQDWINSTERQEETRKAPHKPGVGSEESLVPYSKPSFPSPGGHSSSGTAS SKGSTGPRKAEILRGSHQRNANDLLDIGYVGSNSQGQFTGEL SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_032106
ORF Size	4536 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_032106.2, NP_115289.1
RefSeq Size	8977 bp
RefSeq ORF	4539 bp
Locus ID	84409
MW	165.7 kDa
Gene Summary	repulsive axon guidance receptor for the slit protein ligands; plays a role in axon guidance and repulsion [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.