Abca8a (NM_001281824) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217668 representing NM_001281824 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAAGAGAGAGATAAACGTGTGGCAACAAACATGGGCCTTATTGTGCAAGAATCTTCTCAGAAAAA AGAGACTGAAAAGAGACACTCTTTGGGAACTCCTGTACACAGCATTTATACTACTTTGTTTATCTTTATT TTTTGAATTGCATGACGTTTATGAGTTTTCGTCACTGTCTGATGTGGACTTGGGACGCGTTGATTCATTT AATGACTCCACCTTTGCGATTGCATACACACCTGTCACCCCAACAACTCAGGAGATAATGAACCGTGTCT CTTTGGTCTCTTACATGACAGGAAGAGAAATATTGGCTTTTCCAAACAAAGAAAATATGACAGAGTTCAT TTCAAAACATTATGATGATGCGGTGGGGATCATATTCTCCACCGCTTACTCCTACCATCTGAAGTTTATG AAAGGAACCAGGATCCCAATCATAAAGGAGCATCAGGACCACACAGCTCATTGTTTTCGATATGAACCTG CCATTTCTTGTGACCTGTCAGCCTTCTGGAGACATGGGTTTGTGGCTCTTCAAGCTGCCATTAACTCCGC CATCATAGAAGTCACAACAAATCACTCAGTGATGGAAGAGATGATGTCACTTACCGGAAAATACATCAAG ATAGACTCTTTCATTGCGCAAGAAGGAACGACGACCGACTACTTCCTTTTTTTATGTATTATTGCTTTCT CCCCACTTACCTACTATATCTCAGCTGGTGTTACAAGAGAAAGGAAGAAGATGAAGGCCTTTATGACAGT GATGGGCCTTCGGGACTCAGCATTCTGGCTGTCCTGGGGTTTGCTCTATGGCGTCATTGTCTTCATCGTG ACCCTTTTCCTGACATTCGTTGTATATTCCGTCCAATTTGTCTTTATGACTGGATTTATGATGATCTTCA GTCTCTTTTTCTTCTATGGCTTATCTCTGATCTCTTTGTCTTTCTTAATGAGTGTCTTACTAAAGAAGTC TTTCCTCACCGACTTGGTGGTGTTCTTCCTCACTGTTTCCTGCGGAAGTCTTGGATTCGCAGCATTATAC AGATACCTTCCTGTGTCCTTGGAGTGGCTTCTAAGTTTGCTAAGCCCTTTCGCTTTTATGCTTGGAATGA CCCAGCTCTTGCGCTTGGACTATGATCTGGAGTCAAGCGGGCACCCCACAGGCAACCCCAATGTAGTCAT TGCCACCGTTTTCTTGTTGTTCTTTGATGGTGTCTTCTACCTGATGTTGACATTCTACTTTGAGAAGGTT TTACCGGGTGAATATGGACGTCGACACCCACCTTTGTTTTTCTTGAAGTCCTCATTTTGGACAGGACAAA ATCCAGCAGATCGGACTGTCCTTGACAATGAAAGGGACTATGAGTTTCCTGATGACTCTTTTGAACCTGT GTCTACAGACTTCCATGGGAAGGAAGTCATCAGAATCAGAAATCTTACAAAAGAATACATACAGAGGTCT AAGCGCACAGAAGCTTTGAAAGATTTGACATTGGATGTATATAAAGGCCAGATTACTGCAATCCTTGGTC ACAGTGGAGCTGGGAAATCAACGCTATTAAATGTCCTTAGTGGACTGTGTGTTCCCACCAAAGGTTGGGT CACTATCCATGATAACAAACTCTCAGAAATGACTGACTTGGAAAGCATCAGCAAGCTCACTGGAGTTTGT CCGCAGTGCAATGTACAATTTGACTTCCTCACTGTAAGAGAAAATCTTAGGCTTTTTGCTAAAATAAAAG GGATTCAAGAAGTGGATAATGAGGTACAAAGGGTTTTGCTGGCACTGGAGATGAAAAATATTCAGAATAC TCTTGTTCAAAACCTAAGTGGTGGACAGAAGAGAAAGCTGACATTTGGAATCGCCATTTTAGGAGACCCT CAGATTTTCCTCCTGGATGAGCCCACTGCTGGGCTGGATCCCTTTTCAAGGCACCGGGTGTGGAATCTCT TGAAGGAGCGCAAAGCAGACCGCGTGGTCCTCTTCAGCACACAGTTCATGGATGAAGCTGACATCCTGGC TGATAGGAAAGTGTTCATCTCCCAGGGGAAGCTGAGGTGTGCAGGATCTTCCTTGTTTCTGAAGAAAAAA TGGGGGATTGGTTATCACTTAAGCTTGCAGCTGAGTGAAACGTGTGTGCACGAAAGAATAACATCGCTCG TCAAACAGCACATCCCCGATTCCAAGTTGTCGGCAGAAAGCGAAGGGAAACTGAATTACATATTACCCCT GGAAAGGACAAACAAATTTCCAGATCTTTATAGGGATCTCGAGAGATGCCCCGACCTGGGAATTGAGAAC TATGGCGTTTCTGTAACAACTCTGACTGAGGTCTTTCTGAAACTGGAAGGAAAATCAGCAATTGATTCAG ATATTGGTGAGACAGAAGATGTACAAGCTGGAGGGACAAGAGGCCCAGGGAGACTTGATGGGGAGCAATT TGTCTCTTTACTTAATGAGATGAGTAAGACAAAAGGGGGCATGGCTCTCTGGTGGCAGCAACTCTGTGCA GTGACCAGGCTTCGCTTCTTGAAGTTAAAGTATAGAAGGAAATCCATCGTTATTCTGATTCTGATTTTGG GGATTGGACTTCTGCATATTCTGTCAGCAAATGTTCACAGGTTAGTCAATCAGAGTGATTACTGTTGGGA GCTCTCTCCTCATACGTACTTCCTCACACCTGGCCAACGACCACAGATGCCCCTCACAAACTTACTGATC ATCAACAAAACAGGAGAAAAGATCGATGACTTCCTACATTCGCTGGAGCAACAGAACATAGCTTTGGAAG TTGATGCCTTTGGGACTAGAAACGGCACAGAGGATAGCCAGTATAATGGCGCTATCATCGTAGCGGGCGG TGAGAAGAATTACAACTTTACATTGGCATGCAATACCAAGAGGCTGAATTGTTTTCCCGTTCTGGTGGAC ATAGTGAGTAATGCGCTACTCGGACTGTTCACACCATCAGTGCACATTCAAACTGATCGCAGCTCATTTC CTGAGGATACAGGCTACACACAGTTTTACCACTTGCTTTACATGTTCTCATGGATCCTTTTGATGGCCTG CGTCCCTCCTTACATTTCCATGACCAGCATTGACGACTATAAGAACAGAGCTCAGTTCCAGCTGTGGATT TCAGGGCTCTCCCCTTCTGCATACTGGTTTGGTCATGCTCTTTTTGAGATCCCAGTCTACTCTGCACTCA TTTTGTCTGTGTACATTGCTTTCTATTTCTCGGCCCCTCCAGAGAGTATGATCACAACTAGAGATCTGTT TATTCAGATCCTCTATGCTGGTGGCTATGCTATGTCAGTTATTTTCATGATGTATGTGATTTCATTCATT TGTCGCAAGGGGAGAAAAAATAGTGGCATTTGGTCACTTTGCTTCTATATTGTCTCTTTCATCTCTCTAT GTCTTATGTTATTTAAATTCTTTGAAGATACCTTTTTGTTTTCTTCTATTGCCCTGATTCCAGTTGTTAC ACTGGTAGGGTGTACATTATTACATTTTCAGAATCATGAGTTTCCTGAAATAAGTTTGTACCCAGAAAGG TACTATACCTATCTGTTTTTCCTAGCTCCTTTCTTTCATTTTCTCATCTTCCTATTTATTCTTCGATGTC TGGAAAGGAAGTTTGGAATGAAAACAATGAGAATGGATCCTGTCTTTAGAATTTCTTCAAGAAGTGATCG TGTATTTAACAATCCAGAGGAACCAGAAGGGGAAGACGAAGATGTGAGTCTAGAAAGAGTGAGGACGGCA AACGCTTTGACGTCAGCGGACTTCCAGGAGAAGCCAGTCATCATTGCCAGCTGCCTTCGTAAAGAGTACA AGGGAAAGAAGAAATGCTTCGTTCTTAAGGGCAAGAAGAAGATAGCCACAAGGAACATCTCTCTTTGCGT TCGCAAAGGCGAGGTGGTCGGATTACTGGGACACAATGGAGCTGGGAAAAGTACATCCATTAAGATGATA ACTGGGGAAATCAAGCCAAGTGCTGGACAGGTGCAGCTGAAAGGGAACAGCACAGGGGACACCCCGGAAT TCCTAGGGTACTGTCCTCAGGAAAATGCTCTGTGGCAACACATGACTGTGAGGGAACACCTGGAGATATT TGCCGCCATAAAGGGGATGAGCAAAAGCGATGCTGCCGTGGCCATTGCAAGGTTGGCAGATGCACTCAAG CTGCAGGACCAGCTGAAGTCTCCGGTGAAGACGCTGTCAGAGGGAGTGAAGAGAAAGCTGTGCTTTGTGC TGAGCATCCTGGGAAACCCTTCTGTGGTGCTTCTGGATGAGCCATCCACCGGGATGGACCCCGAGGGGCA GCAGCAAATGTGGCAGGCGATCCAGGCCACCTTCGCAAACACAGAGAGGGGCGCCCTCCTGACCACCCAT TACATGGCAGAGGCTGAGGCTGTGTGTGACCGCGTGGCCATCATGGTGTCTGGGAAGCTGAGATGTATTG GATCCATCCAACATCTAAAGAGCAAATTTGGCAAAGAATATCTCCTGGAGATGAAGGTGAAGACCCCCAC ACAGGTGGAGCCCCTCAACATGGAGATCATGAGGCTCTTCCCCCAGGCTGCTCGGCAGGAAAGGTACTCT TCTCTGATGGTCTATAAACTGCCCAGGGAAGATGTGCAACCTTTATCCCAGGCCTTTTTCAAATTAGAGA CAGTTAAACAGAGCTTTGACCTGGAGGAGTACAGCCTCTCACAGTCCACTCTGGAGCAGGTCTTCCTGGA ACTCTCCAAGGAACAGGAGCTGGATGATTTTGAAGAGGAACTCGATCCCTCAGTGAAGTGGAAGCTCCTC CCACAGGAAGAGGCT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217668 representing NM_001281824 Red=Cloning site Green=Tags(s) MVKREINVWQQTWALLCKNLLRKKRLKRDTLWELLYTAFILLCLSLFFELHDVYEFSSLSDVDLGRVDSF NDSTFAIAYTPVTPTTQEIMNRVSLVSYMTGREILAFPNKENMTEFISKHYDDAVGIIFSTAYSYHLKFM KGTRIPIIKEHQDHTAHCFRYEPAISCDLSAFWRHGFVALQAAINSAIIEVTTNHSVMEEMMSLTGKYIK IDSFIAQEGTTTDYFLFLCIIAFSPLTYYISAGVTRERKKMKAFMTVMGLRDSAFWLSWGLLYGVIVFIV TLFLTFVVYSVQFVFMTGFMMIFSLFFFYGLSLISLSFLMSVLLKKSFLTDLVVFFLTVSCGSLGFAALY RYLPVSLEWLLSLLSPFAFMLGMTQLLRLDYDLESSGHPTGNPNVVIATVFLLFFDGVFYLMLTFYFEKV LPGEYGRRHPPLFFLKSSFWTGQNPADRTVLDNERDYEFPDDSFEPVSTDFHGKEVIRIRNLTKEYIQRS KRTEALKDLTLDVYKGQITAILGHSGAGKSTLLNVLSGLCVPTKGWVTIHDNKLSEMTDLESISKLTGVC PQCNVQFDFLTVRENLRLFAKIKGIQEVDNEVQRVLLALEMKNIQNTLVQNLSGGQKRKLTFGIAILGDP QIFLLDEPTAGLDPFSRHRVWNLLKERKADRVVLFSTQFMDEADILADRKVFISQGKLRCAGSSLFLKKK WGIGYHLSLQLSETCVHERITSLVKQHIPDSKLSAESEGKLNYILPLERTNKFPDLYRDLERCPDLGIEN YGVSVTTLTEVFLKLEGKSAIDSDIGETEDVQAGGTRGPGRLDGEQFVSLLNEMSKTKGGMALWWQQLCA VTRLRFLKLKYRRKSIVILILILGIGLLHILSANVHRLVNQSDYCWELSPHTYFLTPGQRPQMPLTNLLI INKTGEKIDDFLHSLEQQNIALEVDAFGTRNGTEDSQYNGAIIVAGGEKNYNFTLACNTKRLNCFPVLVD IVSNALLGLFTPSVHIQTDRSSFPEDTGYTQFYHLLYMFSWILLMACVPPYISMTSIDDYKNRAQFQLWI SGLSPSAYWFGHALFEIPVYSALILSVYIAFYFSAPPESMITTRDLFIQILYAGGYAMSVIFMMYVISFI CRKGRKNSGIWSLCFYIVSFISLCLMLFKFFEDTFLFSSIALIPVVTLVGCTLLHFQNHEFPEISLYPER YYTYLFFLAPFFHFLIFLFILRCLERKFGMKTMRMDPVFRISSRSDRVFNNPEEPEGEDEDVSLERVRTA NALTSADFQEKPVIIASCLRKEYKGKKKCFVLKGKKKIATRNISLCVRKGEVVGLLGHNGAGKSTSIKMI TGEIKPSAGQVQLKGNSTGDTPEFLGYCPQENALWQHMTVREHLEIFAAIKGMSKSDAAVAIARLADALK LQDQLKSPVKTLSEGVKRKLCFVLSILGNPSVVLLDEPSTGMDPEGQQQMWQAIQATFANTERGALLTTH YMAEAEAVCDRVAIMVSGKLRCIGSIQHLKSKFGKEYLLEMKVKTPTQVEPLNMEIMRLFPQAARQERYS SLMVYKLPREDVQPLSQAFFKLETVKQSFDLEEYSLSQSTLEQVFLELSKEQELDDFEEELDPSVKWKLL PQEEA SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001281824
ORF Size	4845 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001281824.1, NP_001268753.1
RefSeq Size	5647 bp
RefSeq ORF	4848 bp
Locus ID	303638
MW	184 kDa

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