Trpm7 (NM_053705) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Chak; LTrpC-7; Ltrpc7; Trp-plik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217703 representing NM_053705 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCAGAAATCCTGGATAGAGAGCACCTTGACCAAGAGGGAGTGTGTGTATATTATACCAAGCTCCA AAGACCCTCACAGATGTCTTCCAGGATGTCAGATTTGTCAGCAACTTGTCAGATGTTTCTGTGGTCGTTT GGTCAAGCAGCATGCATGCTTTACTGCAAGTCTTGCCACAAAATACTCAGATGTGAAATTGGGTGAACAC TTTAACCAGGCAATAGAAGAATGGTCTGTGGAAAAGCACACGGAGCAGAGCCCAACAGATGCTTATGGAG TCATCAATTTTCAAGGGGGTTCTCATTCCTACAGAGCTAAGTATGTGAGACTATCATATGATACCAAACC TGAAATCATTCTGCAACTTCTGCTTAAAGAATGGCAAATGGAGTTACCCAAACTTGTTATTTCTGTACAT GGAGGCATGCAGAAATTTGAACTTCATCCAAGAATCAAGCAGTTGCTTGGAAAGGGTCTTATTAAAGCTG CAGTTACAACCGGAGCTTGGATTTTAACTGGAGGAGTAAATACAGGTGTGGCAAAACATGTTGGTGATGC CCTCAAAGAACATGCTTCCAGATCATCTCGAAAAATTTGCACTATTGGAATAGCTCCATGGGGAGTGATA GAAAACAGGAATGATCTTGTTGGGAGAGATGTGGTTGCCCCCTATCAAACCCTATTAAATCCCTTGAGCA AATTGAATGTTCTGAATAATCTACACTCCCATTTCATCTTGGTGGATGACGGCACGGTTGGAAAGTATGG GGCAGAAGTCAGACTGAGAAGAGAACTTGAAAAAACCATTAATCAGCAAAGAATTCATGCTAGAATTGGG CAAGGTGTTCCTGTGGTGGCTTTGATATTTGAAGGCGGGCCAAATGTCATCCTTACAGTACTGGAGTACC TCCAGGAAAGCCCCCCCGTTCCAGTTGTTGTGTGTGAAGGGACAGGCAGAGCTGCAGATTTACTAGCATA TATCCATAAGCAGACAGAGGAAGGAGGGAATCTTCCTGATGCAGCAGAGCCTGATATTATATCAACTATC AAAAAAACATTTAACTTTGGCCAGAGTGAAGCAGTTCATTTATTTCAAACAATGATGGAGTGTATGAAAA AAAAAGAGCTTATCACTGTTTTCCATATTGGTTCAGAGGACCATCAAGATATAGATGTGGCCATACTTAC TGCACTGCTAAAAGGTACTAATGCATCAGCGTTTGACCAGCTTATCCTTACACTGGCATGGGACAGAGTT GATATTGCCAAAAATCATGTATTTGTTTATGGACAACAGTGGTTGGTTGGATCCTTGGAACAAGCTATGC TTGATGCTCTTGTAATGGATAGAGTTTCATTTGTAAAACTTCTTATTGAAAATGGAGTAAGCATGCATAA ATTCCTTACCATTCCTAGACTGGAAGAACTTTATAACACTAAACAGGGTCCGACTAATCCAATGTTGTTC CACCTCATTCGGGATGTCAAACAGGGTAATCTTCCTCCAGGGTACAAGATCACTCTAATTGATATAGGAC TTGTGATTGAATATCTCATGGGAGGAACCTACAGATGCACATACACACGAAAACGTTTTCGCTTAATATA TAATAGTCTTGGTGGAAATAACCGGAGGTCAGGTCGAAATGCTTCCAGCAGCACCCCTCAGTTGCGAAAG AGTCATGAAACTTTTGGCAATAGAGCTGATAAAAAGGAAAAAATGAGACACAATCATTTCATTAAAACAG CCCAACCCTACAGACCAAAGATGGATGCATCTATGGAAGAAGGGAAGAAGAAACGAACCAAAGATGAAAT TGTAGATATAGATGATCCAGAAACCAAGCGCTTTCCTTATCCTCTTAATGAATTATTGATCTGGGCTTGC CTTATGAAGAGGCAAGTCATGGCCCGCTTTTTATGGCAGCATGGTGAGGAATCAATGGCTAAAGCATTAG TTGCCTGTAAAATCTATCGTTCAATGGCTTATGAGGCAAAACAGAGTGACCTGGTAGATGATACTTCAGA GGAACTGAAACAGTATTCCAATGATTTTGGTCAACTCGCCGTTGAATTACTGGAACAGTCCTTCAGACAA GATGAAACAATGGCTATGAAATTACTCACTTATGAACTGAAAAACTGGAGTAATTCAACCTGCCTCAAGT TAGCAGTTTCTTCAAGGCTTAGGCCTTTTGTAGCTCACACTTGTACACAGATGCTGTTGTCTGATATGTG GATGGGACGGCTGAATATGAGAAAAAATTCCTGGTATAAGGTTATATTAAGCATTTTAGTTCCACCTGCC ATATTAATGCTAGAGTATAAAACCAAGGCTGAAATGTCCCATATCCCACAATCTCAAGATGCTCATCAAA TGACCATGGAGGATAGTGAAAACAATTTTCACAACATAACAGAAGAGATACCCATGGAAGTATTTAAAGA AGTAAAGATTTTGGACAGCAGTGAAGGAAAGAATGAAATGGAGATACATATTAAATCAAAAAAGCTTCCA ATCACACGAAAATTTTATGCCTTTTATCACGCGCCAATTGTAAAGTTCTGGTTTAACACATTGGCATACT TAGGATTTCTGATGCTTTATACATTTGTAGTTCTTGTACAAATGGAACAGTTACCTTCAGTTCAAGAGTG GATTGTTATCGCTTATATTTTTACCTATGCTATTGAAAAAATTCGTGAGGTCTTTATGTCTGAAGCTGGG AAAATCAGCCAGAAGATCAAAGTGTGGTTTAGTGACTACTTCAATGTCAGTGACACAATTGCCATAATTT CTTTCTTTGTTGGATTTGGACTAAGATTTGGAGCAAAATGGAACTATATTAATGCATATGATAATCATGT TTTTGTTGCTGGAAGATTAATTTACTGTCTTAATATAATATTTTGGTATGTGCGCTTGCTAGATTTTCTA GCTGTAAATCAACAGGCAGGACCTTATGTAATGATGATTGGAAAAATGGTGGCCAATATGTTCTACATTG TAGTGATAATGGCTCTTGTATTACTTAGTTTTGGTGTTCCCAGAAAAGCAATACTTTATCCACATGAAGA ACCGTCTTGGTCTCTTGCTAAAGATATAGTTTTTCATCCATACTGGATGATTTTTGGTGAAGTTTATGCG TATGAAATTGATGTGTGTGCAAATGATTCCGCTCTCCCGACAATCTGTGGTCCTGGAACTTGGTTGACTC CATTTCTTCAAGCAGTCTACCTCTTTGTACAGTATATCATTATGGTTAATCTTCTTATTGCATTTTTCAA TAATGTATATTTACAAGTGAAGGCAATTTCCAATATTGTATGGAAGTATCAGCGATATCATTTCATTATG GCTTATCATGAAAAACCAGTCCTGCCTCCTCCACTTATCATTCTCAGCCATATAGTTTCACTGTTTTGCT GTATCTGTAAAAGAAGAAAGAAAGATAAGACTTCTGATGGGCCAAAACTTTTCTTAACAGAAGAAGATCA AAAGAAACTTCATGACTTTGAAGAACAGTGTGTTGAGATGTATTTTGATGAGAAAGATGACAAATTCAAT TCTGGAAGTGAAGAGAGAATCCGTGTCACTTTTGAAAGAGTGGAGCAGATGAGCATTCAGATTAAAGAAG TTGGAGATCGTGTCAACTACATAAAAAGATCGTTACAGTCTTTAGATTCTCAAATTGGTCACCTGCAAGA TCTTTCAGCCCTAACAGTAGATACATTAAAAACACTTACAGCCCAGAAAGCTTCAGAAGCTAGCAAAGTG CACAATGAGATTACACGAGAATTGAGTATTTCCAAACACTTGGCTCAGAATCTTATTGATGATGTTCCTG TAAGGCCTATGTGGAAGAAGCCTAGTGTTGTAAATACACTGAGTTCCTCTCTTCCTCAAGGTGATCGGGA AAGTAATAATCCTTTTCTTTGTAATATTTTTATGAAAGATGAAAAAGACCCCCAGTATAACCTGTTTGGA CAGGATTTGCCTGTGATACCCCAGAGAAAAGAATTCAACATTCCAGAGGCTGGCTCCTCCTGTGGTGCCT TATTCCCAAGTGCTGTTTCTCCCCCAGAATTACGACAAAGACGACATGGGGTAGAAATGTTAAAAATATT TAATAAAAATCAAAAATTAGGCAGTTCACCTAATAGTTCACCCCATATGTCCTCCCCACCAACCAAATTT TCTGTGAGTACCCCATCCCAGCCAAGTTGCAAAAGCCACTTGGAATCCACAACCAAAGATCCAGAACCCA TTTTCTATAAAGCTGCAGAAGGGGATAACATAGAATTTGGAGCATTTGTGGGACACAGAGATAGTATGGA TTTACAGAGGTTTAAAGAAACATCAAACAAAATAAGAGAACTGTTATCTAATGATACTCCTGAAAACACT TTGAAACATGTGGGTGCTGCTGGATATAATGAATGTCATAAGACTCCTACTTCTCTTCATTCAGAACAAG AGAGCTGTAGTAGAAGAGCGTCGACAGAAGACTCTCCGGACGTAGATTCCAGAGCAGCTTTGTTGCCGGA TTGGTTACGAGATAGACCAACAAACAGAGAAATGCCATCTGAAGGAGGAACATTAAATGGTCTTGCTTCT CCATTTAAACCTGTTTTGGATACAAATTACTATTATTCAGCTGTGGAAAGAAATAACCTGATGAGGCTGT CGCAGAGTATTCCCTTCGTTCCTGTACCTCCACGAGGTGAGCCTGTCACAGTATATCGTCTGGAGGAGAG TTCTCCCAGTATACTGAATAACAGCATGTCTTCTTGGTCTCAGCTAGGCCTCTGTGCCAAAATTGAGTTT TTAAGTAAAGAGGAAATGGGAGGCGGTTTACGAAGAGCAGTCAAAGTGCTGTGTACCTGGTCAGAGCACG ATGTCCTGAGATCAGGGCACCTTTATATCATTAAGTCGTTTCTTCCAGAGGTGATAAACACATGGTCAAG CATTTATAAAGAAGACACAGTTCTGCATCTCTGTCTCAGAGAAATACAACAACAGAGAGCAGCACAGAAG CTCACATTTGCCTTTAATCAGATGAAACCCAAATCCATACCATATTCTCCAAGGTTCCTGGAAGTTTTCC TGCTATACTGCCATTCAGCAGGGCAGTGGTTTGCTGTAGAAGAATGCATGACTGGGGAATTTAGGAAATA CAACAATAATAATGGTGATGAAATCATTCCAACCAACACTCTGGAAGAGATCATGCTAGCCTTCAGCCAC TGGACCTATGAGTACACCAGAGGGGAGTTGCTGGTACTTGACTTACAAGGTGTGGGAGAAAACTTGACTG ACCCATCTGTGATAAAAGCTGAAGAGAAAAGATCCTGCGACATGGTTTTTGGCCCTGCCAATTTAGGAGA AGATGCAATAAAAAACTTCAGAGCCAAACATCACTGTAATTCTTGCTGTCGAAAGCTTAAACTTCCAGAT CTGAAGAGGAATGACTACACACCTGATAAAATTATATTTCCTCAGGATGAGTCATCAGATCTGAATCTTC AAGCTGGAAATTCTACCAAAGAATCAGAAGCAACAAATTCTGTTCGTCTGATGTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217703 representing NM_053705 Red=Cloning site Green=Tags(s) MSQKSWIESTLTKRECVYIIPSSKDPHRCLPGCQICQQLVRCFCGRLVKQHACFTASLATKYSDVKLGEH FNQAIEEWSVEKHTEQSPTDAYGVINFQGGSHSYRAKYVRLSYDTKPEIILQLLLKEWQMELPKLVISVH GGMQKFELHPRIKQLLGKGLIKAAVTTGAWILTGGVNTGVAKHVGDALKEHASRSSRKICTIGIAPWGVI ENRNDLVGRDVVAPYQTLLNPLSKLNVLNNLHSHFILVDDGTVGKYGAEVRLRRELEKTINQQRIHARIG QGVPVVALIFEGGPNVILTVLEYLQESPPVPVVVCEGTGRAADLLAYIHKQTEEGGNLPDAAEPDIISTI KKTFNFGQSEAVHLFQTMMECMKKKELITVFHIGSEDHQDIDVAILTALLKGTNASAFDQLILTLAWDRV DIAKNHVFVYGQQWLVGSLEQAMLDALVMDRVSFVKLLIENGVSMHKFLTIPRLEELYNTKQGPTNPMLF HLIRDVKQGNLPPGYKITLIDIGLVIEYLMGGTYRCTYTRKRFRLIYNSLGGNNRRSGRNASSSTPQLRK SHETFGNRADKKEKMRHNHFIKTAQPYRPKMDASMEEGKKKRTKDEIVDIDDPETKRFPYPLNELLIWAC LMKRQVMARFLWQHGEESMAKALVACKIYRSMAYEAKQSDLVDDTSEELKQYSNDFGQLAVELLEQSFRQ DETMAMKLLTYELKNWSNSTCLKLAVSSRLRPFVAHTCTQMLLSDMWMGRLNMRKNSWYKVILSILVPPA ILMLEYKTKAEMSHIPQSQDAHQMTMEDSENNFHNITEEIPMEVFKEVKILDSSEGKNEMEIHIKSKKLP ITRKFYAFYHAPIVKFWFNTLAYLGFLMLYTFVVLVQMEQLPSVQEWIVIAYIFTYAIEKIREVFMSEAG KISQKIKVWFSDYFNVSDTIAIISFFVGFGLRFGAKWNYINAYDNHVFVAGRLIYCLNIIFWYVRLLDFL AVNQQAGPYVMMIGKMVANMFYIVVIMALVLLSFGVPRKAILYPHEEPSWSLAKDIVFHPYWMIFGEVYA YEIDVCANDSALPTICGPGTWLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLQVKAISNIVWKYQRYHFIM AYHEKPVLPPPLIILSHIVSLFCCICKRRKKDKTSDGPKLFLTEEDQKKLHDFEEQCVEMYFDEKDDKFN SGSEERIRVTFERVEQMSIQIKEVGDRVNYIKRSLQSLDSQIGHLQDLSALTVDTLKTLTAQKASEASKV HNEITRELSISKHLAQNLIDDVPVRPMWKKPSVVNTLSSSLPQGDRESNNPFLCNIFMKDEKDPQYNLFG QDLPVIPQRKEFNIPEAGSSCGALFPSAVSPPELRQRRHGVEMLKIFNKNQKLGSSPNSSPHMSSPPTKF SVSTPSQPSCKSHLESTTKDPEPIFYKAAEGDNIEFGAFVGHRDSMDLQRFKETSNKIRELLSNDTPENT LKHVGAAGYNECHKTPTSLHSEQESCSRRASTEDSPDVDSRAALLPDWLRDRPTNREMPSEGGTLNGLAS PFKPVLDTNYYYSAVERNNLMRLSQSIPFVPVPPRGEPVTVYRLEESSPSILNNSMSSWSQLGLCAKIEF LSKEEMGGGLRRAVKVLCTWSEHDVLRSGHLYIIKSFLPEVINTWSSIYKEDTVLHLCLREIQQQRAAQK LTFAFNQMKPKSIPYSPRFLEVFLLYCHSAGQWFAVEECMTGEFRKYNNNNGDEIIPTNTLEEIMLAFSH WTYEYTRGELLVLDLQGVGENLTDPSVIKAEEKRSCDMVFGPANLGEDAIKNFRAKHHCNSCCRKLKLPD LKRNDYTPDKIIFPQDESSDLNLQAGNSTKESEATNSVRLML TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053705
ORF Size	5586 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053705.2, NP_446157.2
RefSeq Size	7118 bp
RefSeq ORF	5589 bp
Locus ID	679906
MW	212.4 kDa
Gene Summary	dual function transmembrane protein; functions as both a calcium-permeant ion channel and serine/threonine protein kinase; implicated in the control of cell division [RGD, Feb 2006]

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