Myo9b (NM_001271067) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217723 representing NM_001271067 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGCTCACGAGGCTGGCAGCTCAGGCCGAAGGCGGCCGGCCACCTTCCACCTGCACATCTACCCTC AGCTGCCCAGTGCTGGGAGCCAGACCTCATGCCGTGTAACCGCCACCAAGGACAGCACAACAAGCGATGT CATCCGAGACGTGGTGGCCAGTCTACACCTGGACGGCTCGAAGCACTATGTGCTGGTGGAGGTGAAGGAG TCGGGTGGGGAGGAGTGGGTGCTGGATGCCAGCGACTCGCCTGTGCACCGAGTGCTGCTGTGGCCTCGGC GAGCGCAGAAGGAGCACCCTCGGGAGGACGGCTACTACTTCCTGCTGCAGGAGCGCAACGCTGACGGCAG CATTCAGTACCTGCACGTGCAGCTGCTGGCTCAGCCCACAGCCGCATGTCGCCTGGTGGAGCGAGGGCTG CTGCCAAGGCCTCAAGCGGACTTCGACGACCTGTGCAACCTGCCGGAGCTGAACGAGGCCAACCTGCTGC AGAGCCTGAAGCTGCGCTTCGTGCAGCAGAAGATCTACACGTACGCGGGCAGCATCCTGGTGGCCATCAA CCCCTTCAAGTTCCTGCCCATTTACAACCCCAAGTATGTGAAGATGTACGAGAACCAGCAGCTGGGAAAG CTGGAGCCGCACGTGTTCGCTCTGGCCGATGTAGCCTACTACACCATGCTGCGCAAGCACGTGAACCAGT GCATCGTCATCTCTGGTGAGAGCGGGTCCGGCAAGACGCAAAGCACCAACTTCCTCATCCACTGCCTCAC AGCGCTCAGCCAGAAGGGCTACGCCAGCGGCGTCGAGAGGACCATCCTGGGTGCAGGGCCTGTGCTGGAG GCTTTTGGGAACGCCAAGACAGCCCACAACAACAACTCCAGCCGCTTCGGGAAGTTCATCCAAGTCAACT ACCTGGAGAGTGGCATCGTGAGGGGAGCTGTTGTGGAAAAATACCTTCTTGAAAAGTCTCGCCTGGTTTC CCAGGAGAAGGATGAGCGGAACTACCATGTGTTTTATTATCTGCTGCTGGGCGTCAGTGAGGAAGAGCGT CAGGAATTTCAGCTGAAGCAGCCTCAAGACTATTTCTACCTCAACCAGCATAACTTGAATATTGAAGATG GAGAAGACCTCAAACATGACTTTGAAAGGCTTCAGCAGGCCATGGAGATGGTGGGCTTCCTACCTGCCAC GAAGAAGCAGATCTTCTCTGTCCTCTCAGCCATCCTGTATCTTGGCAATGTCACCTATAAGAAGAGAGCC ACAGGCCGGGATGAAGGCCTGGAGGTCGGGCCCCCGGAGGTATTGGACACACTATCCCAGCTCCTGAAGG TAAAGCGGGAGACCCTGGTGGAGGTCCTAACCAAGAGAAAAACAATCACGGTCAATGACAAACTCATCCT GCCTTACAGCCTCAGTGAGGCTATCACTGCACGAGACTCCATGGCTAAGTCTCTGTACAGCGCCCTGTTT GACTGGATTGTGCTGAGGATCAACCACGCCCTCCTCAACAAGAAGGACATGGAAGAGGCTGTTTCTTGCT TGTCCATTGGCGTCCTGGACATATTCGGATTTGAGGACTTTGAACGGAACAGCTTCGAGCAGTTTTGCAT CAACTACGCCAACGAGCAGTTGCAGTACTACTTCACCCAGCACATCTTCAAGCTGGAGCAGGAGGAGTAC CAGGGTGAGGGTATCTCGTGGCACAACATTGACTACACCGACAACGTGGGCTGTATCCACCTCATCAGCA AGAAGCCCACTGGCCTCTTCTACCTGCTGGACGAGGAAAGCAACTTTCCCCATGCCACAAGCCACACTCT GCTGGCCAAATTCAAGCAGCAGCATGAGGACAACAAGTACTTCCTGGGCACACCAGTCCTGGAGCCCGCC TTCATCATCCAGCACTTCGCAGGCAGAGTAAAGTACCAGATCAAGGACTTCCGGGAGAAGAACATGGACT ACATGCGGCCTGACATCGTGGCACTGCTAAGGGGCAGTGACAGCTCCTATGTGCGCCAACTCATCGGCAT GGACCCGGTAGCTGTGTTCCGCTGGGCTGTATTACGGGCAGCCATCAGGGCCATGGCTGTGCTGCGGGAG GCTGGGCGCCTGCGTGCAGAGAGAGCAGAGAAAGCAGAAGCAGGTGTAAGTAGTCCTGTCACTCGAAGTC ACGTGGAAGAGCTGCCAAGAGGAGCCAACACCCCTTCAGAGAAACTGTACCGCTGCGCAGGGCTAGACTT CTCCTTTGAGCGCTCTGAGGAGCTGGATGTTAACGCTTTTGAGGACATCATGGCTTTCTATGAGAGCAGG AACGATTTGCATAACCAAATCATCAAGAGCCTCAAAGGACTGCCATGGCAGGGCGAGGACCCGCGGAGGC TTCTCCAGTCCCTCAGTCGGCTCCAGAAGCCCCGCACCTTCTTCCTGAAGAGTAAAGGTATCAAACAAAA GCAGATCATTCCCAAGAACCTGCTGGACTCTAAGTCCCTGAGGCTCATCATCAGCATGACGCTGCATGAC CGAACTACCAAGTCACTGCTGCACCTGCACAAGAAGAAGAAGCCGCCCAGCATCAGTGCACAGTTCCAGA CATCTCTTAACAAGCTGTTGGAGGCGCTGGGCAAGGCTGAGCCCTTCTTCATCCGCTGCATTCGCTCCAA TGCCGAGAAGAAGGAGCTCTGCTTTGATGATGAGCTGGTGCTTCAGCAACTGCGCTACACAGGCATGCTG GAGACCGTGCGCATCCGGCGCTCTGGCTACAGCGCCAAGTACACCTTCCAGGACTTCACGGAGCAGTTCC AGGTGCTGCTGCCCAAGGATGTCCAGCCCTGTAGGGAGGCCATTGCTGCCCTGTTGGAGAAGCTGCAGGT GGACAGGCAGAACTACCAGATCGGGAAGACGAAGGTCTTCCTGAAGGAGACAGAGCGGCAGGCCCTGCAG GAGAGGCTGCATGGTGAGGTCTTACGCAGGATCCTGCTGCTGCAGAGTTGGTTCCGGATGGTGCTGGAAC GCAGGCACTTTGTGCAGATGAAGCATGCTGCCCTGACCATCCAGGCCTGCTGGCGGTCTTATCGTGTGCG CCGTACACTGGAGAGGACGCGGGCAGCTGTGTATCTTCAGGCTGCCTGGAGAGGCTACCTGCAGAGACAG GCCTACCACCACCAGAGGCATAGCATCATCCGCCTGCAGAGCCTCTGCCGTGGCCACCTACAGCGCAGGA GCTTCAGCCAGATGATGTTAGAGAAGCAGAAGGCAGAGCAAGCCAGGGAGACTGCAGGAGCAGAGATGTC AGAGGGAGAGCCCAGCCCTGTGGCCGCTGGGGAGCAGCCGTCTGAGCACCCTGTGGAGGACCCTGAGAGC CTGGGTGTGGAGACTGAGACCTGGATGAACAGCAAGTCCCCAAATGGCTTGTCACCTAAGAAGGAGATCC CCAGCCCGGAGATGGAGACCCCAGCCCAAAAAACAGTGCCAGCTGAAAGTCATGAGAAAGTCCCCAGTAG CCGGGAGAAGCGAGAGTCACGGCGGCAGCGAGGGTTGGAGCATGTTGAACGACAGAACAAACACATCCAA TCCTGCAGGGAGGAGAATAGCACCCTCCGAGAACCTTCCAGAAAGGCAAGCCTGGAAACAGGGGAGAGCT TCCCTGAGGACACAAAGGAGCCCAGAGAAGATGGACTTGAGACATGGACTGAGACCGCAGCCCCCTCTTG TCCAAAGCAGGTGCCGATTGTGGGAGATCCGCCTAGGAGTCCCAGTCCCTTGCAGAGGCCCGCCAGCCTG GACCTAGACAGCAGGGTTAGCCCAGTGCTCCCCAGCAGCTCCCTGGAATCCCCCCAGGATGAGGACAAGG GTGAGAACAGCACCAAGGTTCAGGACAAGCCCGAGAGTCCCAGTGGCTCCACCCAGATCCAACGGTACCA ACACCCGGACACAGAGCGGCTAGCCACTGCTGTGGAGATATGGCGAGGCAAGAAGCTCGCCAGTGCCATG CTGAGCCAATCCCTGGACCTGAGTGAGAAGCCCCGGACTGCAGGGGCAGCCCTGACTCCCACAGAGGAGA GGCGCATCTCCTTCTCCACCAGTGATGTCTCCAAGCTGTCCCCGGTCAAGACTTCAACTGAAGTCGATGG GGATCTGAGCGCCAAGAAGCCAGCCGGCCATAAGAAGAAGTCAGAAGACCCATCTGCTGGTCCCGATGCA GGCCTGCCCACAGGCTCCCAGGGTGACTCTAAATCTGCCTTTAAGCGACTCTTCCTGCACAAAGCCAAGG ATAAGAAGCCCAGCCTGGAGGGTGTGGAGGAGACAGAGGGCAGTGGAGGGCAGGCTGCACAGGAGGCCCC GGCCAGGAAGACTCTAGATGTACCTTCTAGCCAGCAGCACCGCCACACCACAGGCGAGAAGCCTCTAAAA GGGAAGAAGAACCGAAATCGTAAGGTCGGACAGATCACAGTGTCCGAGAAGTGGCGAGAGTCGGTGTTCC GTAAGATCACTAATGCCAACGAGCTCAAGTTTCTGGATGAGTTCCTGCTCAACAAGGTGAATGACCTTCG CTCACAGAAGACACCCATCGAGAGCTTGTTCATTGAGGCCACTGAGCGTTTCAGGAGCAATATCAAGACC ATGTATTCTGTGCCTAACGGGAAGATCCATGTAGGCTACAAGGACTTGATGGAGAACTACCAGATCGTTG TCAGTAACCTGGCTGCCGAGCGTGGGGAGAAGGACACCAACCTGGTCCTCAATGTCTTCCAGTCACTGCT GGATGAATTCACTCGCAGCTACAACAAGACTGACTTTGAGCCGGTCAAGGGCAAAGCCCAGAAGAAGAAG AGGAAGCAGGAGCGTGCTGTCCAGGAACACAATGGACATGTGTTTGCCAGCTACCAAGTGAACATCCCGC AGTCGTGTGAGCAGTGTCTGTCCTACATCTGGCTCATGGACAAGGCCCTGCTATGCAGTGTGTGCAAGAT GACCTGCCACAAGAAATGTGTGCACAAGATTCAGAGCTATTGCTCCTACACTGGACGGAGGAAGAGTGAG CTGGGTGCCGAACCAGGCCACTTCGGCGTGTGTGTAGACAGCCTGACCAGTGACAAGGCCTCCGTGCCCA TTGTGCTGGAGAAGCTTCTGGAACATGTAGAGATGCATGGCCTGTATACCGAGGGTCTTTACCGCAAGTC AGGCGCTGCCAACCGGACACGGGAATTACGCCAGGCTCTGCAGACAGACCCTGCTACAGTTAAGCTGGAG GACTTCCCCATCCACGCCATCACCGGGGTCCTGAAGCAATGGCTGCGGGAGCTGCCTGAGCCACTCATGA CTTTTGCCCAGTATGGAGACTTCCTCAGGGCTGTTGAGCTTCCAGAGAAGCAGGAGCAGCTGGCTGCCAT CTATGCAGTCCTGGACCACCTGCCTGAAGCCAACCACACCTCCCTGGAGAGGCTCATCTTCCACCTTGTC AAAGTGGCCCTGCTTGAAGATGTGAACCGCATGTCTCCGGGAGCTCTAGCTATCATCTTCGCGCCCTGCC TGCTTCGCTGCCCTGACAACTCCGACCCCCTGACCAGCATGAAGGACGTACTAAAGATTACCACGTGTGT GGAGATGCTCATCAAGGAACAGATGAGGAAATACAAGGTGAAGATGGAGGAGATCAACCACCTGGAGGCA GCTGAGAGCATTGCGTTCCGCAGGCTCTCCCTGCTGAGGCAGAATGCCCCGTGGCCTCTCAAACTGGGGT TTTCGTCTCCCTATGAGGGGGTCCGGACCAAAAGCCCCAGGACCCCAGTGGTCCAAGACTTGGAGGAGCT GGGGGCTCTCCCCGAGGAGGCGGCAGGTGGTGACGAGGACCGGGAAAAGGAGATTCTCATGGAGAGGATT CAGTCCATCAAAGAAGAGAAGGAGGACATCACATATCGACTGCCGGAGCTGGACCCACGGGGTTCTGACG AGGAGAACCTTGACTCAGAGACATCAGCCAGCACTGAGAGCCTGCTGGAGGAGAGGGCCGTGCGGGGGGC CGCAGAAGGTCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217723 representing NM_001271067 Red=Cloning site Green=Tags(s) MSAHEAGSSGRRRPATFHLHIYPQLPSAGSQTSCRVTATKDSTTSDVIRDVVASLHLDGSKHYVLVEVKE SGGEEWVLDASDSPVHRVLLWPRRAQKEHPREDGYYFLLQERNADGSIQYLHVQLLAQPTAACRLVERGL LPRPQADFDDLCNLPELNEANLLQSLKLRFVQQKIYTYAGSILVAINPFKFLPIYNPKYVKMYENQQLGK LEPHVFALADVAYYTMLRKHVNQCIVISGESGSGKTQSTNFLIHCLTALSQKGYASGVERTILGAGPVLE AFGNAKTAHNNNSSRFGKFIQVNYLESGIVRGAVVEKYLLEKSRLVSQEKDERNYHVFYYLLLGVSEEER QEFQLKQPQDYFYLNQHNLNIEDGEDLKHDFERLQQAMEMVGFLPATKKQIFSVLSAILYLGNVTYKKRA TGRDEGLEVGPPEVLDTLSQLLKVKRETLVEVLTKRKTITVNDKLILPYSLSEAITARDSMAKSLYSALF DWIVLRINHALLNKKDMEEAVSCLSIGVLDIFGFEDFERNSFEQFCINYANEQLQYYFTQHIFKLEQEEY QGEGISWHNIDYTDNVGCIHLISKKPTGLFYLLDEESNFPHATSHTLLAKFKQQHEDNKYFLGTPVLEPA FIIQHFAGRVKYQIKDFREKNMDYMRPDIVALLRGSDSSYVRQLIGMDPVAVFRWAVLRAAIRAMAVLRE AGRLRAERAEKAEAGVSSPVTRSHVEELPRGANTPSEKLYRCAGLDFSFERSEELDVNAFEDIMAFYESR NDLHNQIIKSLKGLPWQGEDPRRLLQSLSRLQKPRTFFLKSKGIKQKQIIPKNLLDSKSLRLIISMTLHD RTTKSLLHLHKKKKPPSISAQFQTSLNKLLEALGKAEPFFIRCIRSNAEKKELCFDDELVLQQLRYTGML ETVRIRRSGYSAKYTFQDFTEQFQVLLPKDVQPCREAIAALLEKLQVDRQNYQIGKTKVFLKETERQALQ ERLHGEVLRRILLLQSWFRMVLERRHFVQMKHAALTIQACWRSYRVRRTLERTRAAVYLQAAWRGYLQRQ AYHHQRHSIIRLQSLCRGHLQRRSFSQMMLEKQKAEQARETAGAEMSEGEPSPVAAGEQPSEHPVEDPES LGVETETWMNSKSPNGLSPKKEIPSPEMETPAQKTVPAESHEKVPSSREKRESRRQRGLEHVERQNKHIQ SCREENSTLREPSRKASLETGESFPEDTKEPREDGLETWTETAAPSCPKQVPIVGDPPRSPSPLQRPASL DLDSRVSPVLPSSSLESPQDEDKGENSTKVQDKPESPSGSTQIQRYQHPDTERLATAVEIWRGKKLASAM LSQSLDLSEKPRTAGAALTPTEERRISFSTSDVSKLSPVKTSTEVDGDLSAKKPAGHKKKSEDPSAGPDA GLPTGSQGDSKSAFKRLFLHKAKDKKPSLEGVEETEGSGGQAAQEAPARKTLDVPSSQQHRHTTGEKPLK GKKNRNRKVGQITVSEKWRESVFRKITNANELKFLDEFLLNKVNDLRSQKTPIESLFIEATERFRSNIKT MYSVPNGKIHVGYKDLMENYQIVVSNLAAERGEKDTNLVLNVFQSLLDEFTRSYNKTDFEPVKGKAQKKK RKQERAVQEHNGHVFASYQVNIPQSCEQCLSYIWLMDKALLCSVCKMTCHKKCVHKIQSYCSYTGRRKSE LGAEPGHFGVCVDSLTSDKASVPIVLEKLLEHVEMHGLYTEGLYRKSGAANRTRELRQALQTDPATVKLE DFPIHAITGVLKQWLRELPEPLMTFAQYGDFLRAVELPEKQEQLAAIYAVLDHLPEANHTSLERLIFHLV KVALLEDVNRMSPGALAIIFAPCLLRCPDNSDPLTSMKDVLKITTCVEMLIKEQMRKYKVKMEEINHLEA AESIAFRRLSLLRQNAPWPLKLGFSSPYEGVRTKSPRTPVVQDLEELGALPEEAAGGDEDREKEILMERI QSIKEEKEDITYRLPELDPRGSDEENLDSETSASTESLLEERAVRGAAEGH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271067
ORF Size	6033 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271067.1, NP_001257996.1
RefSeq Size	7077 bp
RefSeq ORF	6036 bp
Locus ID	25486
MW	228.6 kDa
Gene Summary	displays ATP-dependent binding to actin: binds members of the rho family of GTPases; may play a role in formation of the actin cytoskeleton [RGD, Feb 2006]

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