Espl1 (NM_001170602) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217737 representing NM_001170602 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAACTTCAAAGGAGTCAAGTTCGCCACTCTACTTTGCTGCAAGAGAGAGACCCAGGAGTTGCTGC CTGACTTGAAGGAGTTCCTGTCTAGATCTCGAACTGATTCAGTCAGCAGCAGAACCGATGCTGAGAGAAG ACAGGTCTGTGACACCATCCTGAGGGCTTGCACCCAGCAGCTGACTGCCAAGCTTGACGGCCCTGGTCAC CTGAGAAGTATCCTGGACCTGGCAGAGCTGGCCTGTGACGGCTACTTGTTGTCTACCCCACAGCGCCCTC CCCTCTATCTGGAACGCATTCTTTTTATCTTACTTCGGAATGGTTTGACTCAGGGAAACCCAGACGCTGT GCTCCGCCTTGCCCAGCCTCTCCATGCCTGCTTGGTACAGAACTCTCGAGAGGCTGCCCCTCAGGACTAT GAGGCTGTGACCCGGGGTACTTTTTCTCTGCTTTGGAAGGGAGCAGAAGCCCTGTCAGAGCGGCGAGCTG CATTCTCAGCTAGGCTGAAGGCCTTGAGTTTCTTAGTGCTTTTGGAGGATGGCAGCCTCCCTTGTGAGGT CCCTCACTTTGCTTCTCCAACAGCCTGTCGGGTAGTTGCCGCCTATCAGCTGTTTGATGCTACTGGACAA GGTCTAGATGCAGCTGACGCTGACTTCGTCTATGACTTGCTTTCTAGGCATGTGATCAGAGTCTTAGTAG GCGAGGGTGGTAGGGCTTCTGGTCCGCTCTCTCCTCAGAGGGCCCTCTGCCTCTTGGAACTCACCTTGGA ACATTGTCGTCGACTCTGCTGGAACCAGCACCACCACAAGGCTACCATAGCAGTGGAGAAGACTCGAGAT CACCTAGGAAATAGCAATGTAGCTCCCAGCCTCCAACTGTGCCAGATGGGGGTTGAGCTGCTGGAAGCTG TGGAAGAAAGACCAGGGGCAGTGGCCCAGCTCCTGATGAAGGCAGCTGCTATTCTGAACAGCAGTATGGA TGGGCCATCACCCCCGCTCCGGGCATTGTATGACAGCTGCCAGTTCTTCCTTTCAGGCCTGGAGAGAACC ATCAAGAGGCACTATGGAGTTGATGCCATATTGAATCTCTTTGCTTTTCTTGGGGGATACCACTCCCTTG TCCGGCACCTGCGAGAAGCCTCCGAAGCGTCCTCTAAGCAGCAGCAGAGCCTGCTCCAGATGCACTTCCA TACCCTCCACCTCTTCACTGGGATTGTGTATGACTTTGCCCAAGGCTGTCAGGTAGCTGAGCTTGCTCAG CTAGTGGACAGTTGCAGATCTGCTGTTGTCTGGATGTTGGAGGCCTTAGAGGGCCTGTCTGGTGGAGAGC TGGCTGACTACCTGGGCGTGACTGCCTCTTACACCAGCAACTTGGCCTACAGCTTCTTTAGTCAGAAGCT CTATGAGGAGGCTTGTGCCATCTCAGAGCCAGTCTGTCAGCACTTGGGCTTGGCTAAGTCAGGCGCTTGT CCCGAAGTGCCTCCTGAGAAGCTACATAGGTGCTTCCGGCTGCATGTGGAGAGTTTGAAGAAATGGGGTA AACAGGCCCAGGGCTGCAAGATGGTGACCTTATGGCTGGCAGCTCTGAAACCCTACGGTCTCCAACACAT GGTTGAGCCAGTCACTTTCTGGGTTCGGGTCAAAATGGATGCAGCCAAGGCAGGAGACAAGGAGCTACAA CTGCAGACTTTGCGAGACAGCCTAAGTTGCTGGGACCCAGAGATGCAGTCTCTGCTACTGAGGGAGGAGC TGCAGGCCTATAAGGCAGTACGGGCTGACACTGGGCAGGAACGCTTCAACGTCATCTGCGATCTGCTTGA GCTCAGCCCTGAGGACACAGCAGCTGGAGCCTGGGCACGAGCTACCCACCTGGTGGAATTGGCCCAAGTG CTGTGCTACCATAGCTTTGCCCAGCAGACAAACTGCTCTGCCTTGGATGCTATCCAGGAGGCCCTGCAGC TTCTGGAGTCCGTGAGTCCCGAGGCACAGGAGCAGGACCGCCTTCTAGATGACAAAGCACAGGCCCTGCT ATGGCTCTACATCTGCACCTTGGAGGCCAAAATGCAGGAAGGTATCGAGCGGGATCGGAGGGCCCAGGCC CCTAGTAACTTGGAGGAATTTGAAGTCAATGACCTGAACTATGAAGATAAACTGCAGGAAGATCGCTTTC TATACAGTAACATTGCCTTCAATCTGGCTGCTGATGCAGCGCAGTCCAGATGCCTGGACCAGGCCCTGAC TCTGTGGAAGGAAGTGCTCACCAAGGAGCCAGCCCCAGCTGTGCGGTGTCTCCAGCAGACGGCAGCCTCC CTGCAAATCCTGGTGGCCATCTATCAGTTGGTGGCAAAGCCCTTGCAGGCTCTGGAGACCCTCCTGCTCC TTCAGATTGTATCTAAGAGACTACAGGATCATGCAAAGGCAGTCAGCTCTTCCTGCCAGCTTACCCAGCT ACTCTTGAACCTTGGCTGTCCCAGCTATGCCCAGTTGTATCTGGAAGAGGCAGAGTCAAGCCTGAAGAAT CTGGACCAAGCGACTGACACGTGCCAGCTGCTTTCCCTGACCTGTGCTCTGCTTCGAAGCCAGCTCTGCT GGGCTTGCCAGAAGGTGACTGAGGGCGTTTCTCTGTTGCTTTCTGTCCTTCGAGATCCTGCCCTCCAGAA GTCATCCAAGGCTTGGTACTTGCTACGTGTCCAGGCCCTGCAAATTCTGGCTTTCTACCTCAGCCTCTCG TCCAACCTCCTCTCAAGTGCCCTACGAGAGGAGCTCTGGGATCAAGGCTGGCAGACCCCAGAGACAGCAC TGATAGATGCCCACAAGCTTCTGAGAAGCATCATCATCTTGCTGATGGGTGGCGATGTGCTCTCCGTTCA GAAAGCAGCTGTGGAGTCGCCCTTTCTGGATTACGGTGAGAATCTGGTGCAGAAATGGCAAGTTCTCACA GAGGTGCTGACCTGCTCGGAGAGGCTGGTCAGCCGCCTGGGCCGCCTGGGGAACGTGAGTGAAGCCAAGG CCTTTTGCTTGGAGGCCCTAAAACTTACCACCAAGCTGCAGATACCTCGCCAGTGTGCCCTGTTCCTTGT ACTGAAGGGTGAGCTGGAGCTGGCCCGGGGAGACACTGACCTCTGTCAGTCAGACTTGCAGCAGGTTCTG TTCTTGCTCGAGTCTTCCACAGAGTTTGGTGTGGTGACACAGCACCCAGACTCAGTGAAGAGGGTGCACA CGCAGAAAGGGACGCATAAGGCTCAAGTCCCCTGTTCCCCACCTCTACCAGAAGAGGAACCCTTCCTGAG AGGCCCTGCCCTTGAGCTGGTGGCCACTGTGCTCAAGGAGCCCGGTCCCATCCAACCCTCTGGAAACTCC TCCCCGGTCCTCAAGACCAAGGCATCACCCAAGCCTGGCTTCCTGTCTCACTTGCCCAGCTGTGATTGCT TACTGTGTGCCAGTCCTGCCCTCTCCACCGTCTGTCTGCGCTGGGTGTTGGTCACTGCAGGAGTGAGGCT CACCACAGGTCATAAAGCTCAGGGTCTGGATCTGCTGCAAGCTGTGCTGAAGGGTTGCCCTGCAGCCACC AAGCGCTTCACACAGAATCTTCAAACTTCTCTGAATCACAAAGCACCCCCCTCTTGTGTTCCAAGCCTCT TTGATGAGATCATGGCTCAAGTGTACACACACTTGGCATTAGAGTGCCTGAATCAGACATCTGAAAAGAG CCTGGGAAAGGTCCTGGCGTCAGGACTGAAGTTTGTGGCAACACGGATACAATACTTAGAGTTCTGGCAA GCCAGCCTGCTCTTGGTTCAGGCCCTTGCAAAGCTGGCCCGCTTCAGCTGCTGTACTAGCGAACTCTTTG CAAGCGCCTGGGGCTGGCAGCCACCATTAGTAAAAATCCCGCCAGTCCTTGAGCCTTCTAAGATTCGGAG ACAAAAATGCTCTGGACAGGGGCGCCAGCGGGTAGCCTCTGCCCCCTCACCCCTCCATAGCAGCTCTCAG GAAGGTCTGGAAGAAGGGCCACCTTGTACACCTAAGCCCCCAGGCCGTGCTAGGCAAGCCGGCCCTCGTG TCCCTTTCACCATATTTGAAGACATCCACCCCACAAAGAGCAAGCTTAAGGTTCCCTTGGCTCCCAGGGT CCACAGGCGGGCCCAGACTCGACTCAAGGTGAACTTCAGCGATGACAGCGACCTGGAAGACCTTGTCTCA GCTGAAACACAACTTGTGGAGGAACCTAAGAGGCGAGGGACTGCTTCCCGCACCCGGGGTCAGAGTAGGA AGGGCCCTAGCTTAAAGACAGATGCAGCGGTTGCCGTAGGTAGTACCCCTGGACATCCCAGTCTCAGTGG GAGGACTCGGAGGGCCAGGAAGGTGGCTTCAAGACATTGTGAAGGTGTCGGGGCCAGCCTGGGACCCGAA ATCATGAGAAGCATCCCTGAAGAGGAGCTGATGGACAGCCAGCTAGAAAAGAGCTTTGAGATTCTCAGGG CTTCTGATGGGGAAGACTCAGCCTCAGGCGAGAAGGCAGCAGCTGCAGAGCCTGGTCTACCCATAGGAGA GCGTGAGGTGTTGAGACGGGACTCCAGCAAGGCAGAGCGACCTGTCTTGTACCCTGATACAGAGGCCGAT AGTGACCTTAGTCCTTGGCTCCCACCCTTCTCAATTCCTGCACCCATTGATCTTTCTACCCTGGATTCCA TCGCTGACTCATTGAGCATTGCTTTTCGTGGTATTAGTCACTGCCCTCCAAGTGGGCTCTACGCTCACCT CTGCCGCTTTTTGGCCTTGTGTCTGGGCCACCGGGATCCCTATGCCACTGCGTTCCTTGTAGCAGAGTCT GTCTCCATCACCTGCCGTCACCAGCTACTCACCCATTTGCACCGACAGCTCAGCAAGGCCCAGAAGCAGC AAGGCTCACCTGAACTAGCAGAGCATCTACAGAGGCTGGACTTGAAGGAGAGGCCCGGAGGTGTCCCACT GGCCCGCGTGCAGCACCTCTTTTCCTTCAGGGCTTTGGGATCTGGCTGCTTCCCCCAGGCAGAGAAGGAC AGTTTCCAGAAGCGCCTGGCTCTCATCCCCAGTGGGGTGACTGTCTGCGTGTTGGCCCTGGCCACCCTCC AGCCTGGAACCTTGAGCAACACTCTCTTACTGACCCGCCTGGAAAAGGACAACCCCCCAATTACTGTGAA GATCCCCACTGCCCAAAGTAAGTTCCCACTAAGTGCGGTGCTGAAAGAGTTCGATGCCATCCAGAAGGAC CAGAAAGAGAACAGCGGCTGTACTGAGAAACGAGCGTGGTGGACGGGCCGGCTAGCACTAGACCAAAGGA TGGAGGAACTCATTACCTCCCTAGAGGAGCATGTGCTGGGCTGCTGGAGGGGGCTGCTGCTGCCGTGTAG CACCGACCCTGGCCTTTCCCAGGAGGCCTCCCAACTACAAGAGTTGCTGCACGAATGTGGCTGGGAATAT CCTGATTACACTCTCCTAAAAGTCATTCTTAGTGGGGCCAGAATCCTCTCCAGCCAGGATGTTCAGGCCT TGGCATGTGGGCTGTGCCCAGCCCAACCTGAGCGAGCCCAAGTGCTCCTGAGCGAGGCAGTAGGACGGGT ACAGAGCCAGGCAGCCCCTCCGAGTCAACATCTTGTACTGGTGTTAGACAAGGACCTGCAGAAGCTGCCG TGGGAAAGCATGCCATGCCTCCGAGCACTGCCGGTCACCCGGCTGCCCTCCCTCCGCTTCCTGCTCAGCT ACTCAATCGCTAAGGAGGCTGGGGCCTCATCAGTGCTGAGCCAAGGTGTTGATCCACGCAATACCTTCTA TGTGCTAAACCCTCACAGTAACCTGTCGAGCACGGAGGAGAGATTTCGAGCCAGTTTTAGCAGTGAAGCC GGATGGAAAGGCGTGATTGGGGAAGTGCCCAGCCTTGAGCAGGTGCAGGCTGCCCTGACAGAGCATGATT TATATATCTATGCAGGACATGGAGCTGGTGCCCGCTTCCTTGATGGGCAGGCTGTCCTGCGGCTGAACTG CCGGGCAGTGGCCCTACTGTTCGGCTGCAGCAGTGCAGCCCTCGCAGTGCATGGAAACCTGGAGGGAGCT GGTATTGTGCTCAAGTACATCATGGCTGGCTGCCCCTTGTTCCTAGGTAATCTCTGGGATGTGACTGACC GTGATATTGACCGTTACACAGAGGCTTTGCTGCAGGGCTGGCTGGGAGCAGGCCCAGGGGCTCCCCTTCT CCACTATGTCAGCCAAGCCCGCCAGGCCCCACGACTCAAGTATCTTATTGGTGCTGCACCTGTAGCCTAC GGCCTACCTGTCTCTCTACAAACATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217737 representing NM_001170602 Red=Cloning site Green=Tags(s) MRNFKGVKFATLLCCKRETQELLPDLKEFLSRSRTDSVSSRTDAERRQVCDTILRACTQQLTAKLDGPGH LRSILDLAELACDGYLLSTPQRPPLYLERILFILLRNGLTQGNPDAVLRLAQPLHACLVQNSREAAPQDY EAVTRGTFSLLWKGAEALSERRAAFSARLKALSFLVLLEDGSLPCEVPHFASPTACRVVAAYQLFDATGQ GLDAADADFVYDLLSRHVIRVLVGEGGRASGPLSPQRALCLLELTLEHCRRLCWNQHHHKATIAVEKTRD HLGNSNVAPSLQLCQMGVELLEAVEERPGAVAQLLMKAAAILNSSMDGPSPPLRALYDSCQFFLSGLERT IKRHYGVDAILNLFAFLGGYHSLVRHLREASEASSKQQQSLLQMHFHTLHLFTGIVYDFAQGCQVAELAQ LVDSCRSAVVWMLEALEGLSGGELADYLGVTASYTSNLAYSFFSQKLYEEACAISEPVCQHLGLAKSGAC PEVPPEKLHRCFRLHVESLKKWGKQAQGCKMVTLWLAALKPYGLQHMVEPVTFWVRVKMDAAKAGDKELQ LQTLRDSLSCWDPEMQSLLLREELQAYKAVRADTGQERFNVICDLLELSPEDTAAGAWARATHLVELAQV LCYHSFAQQTNCSALDAIQEALQLLESVSPEAQEQDRLLDDKAQALLWLYICTLEAKMQEGIERDRRAQA PSNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSRCLDQALTLWKEVLTKEPAPAVRCLQQTAAS LQILVAIYQLVAKPLQALETLLLLQIVSKRLQDHAKAVSSSCQLTQLLLNLGCPSYAQLYLEEAESSLKN LDQATDTCQLLSLTCALLRSQLCWACQKVTEGVSLLLSVLRDPALQKSSKAWYLLRVQALQILAFYLSLS SNLLSSALREELWDQGWQTPETALIDAHKLLRSIIILLMGGDVLSVQKAAVESPFLDYGENLVQKWQVLT EVLTCSERLVSRLGRLGNVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARGDTDLCQSDLQQVL FLLESSTEFGVVTQHPDSVKRVHTQKGTHKAQVPCSPPLPEEEPFLRGPALELVATVLKEPGPIQPSGNS SPVLKTKASPKPGFLSHLPSCDCLLCASPALSTVCLRWVLVTAGVRLTTGHKAQGLDLLQAVLKGCPAAT KRFTQNLQTSLNHKAPPSCVPSLFDEIMAQVYTHLALECLNQTSEKSLGKVLASGLKFVATRIQYLEFWQ ASLLLVQALAKLARFSCCTSELFASAWGWQPPLVKIPPVLEPSKIRRQKCSGQGRQRVASAPSPLHSSSQ EGLEEGPPCTPKPPGRARQAGPRVPFTIFEDIHPTKSKLKVPLAPRVHRRAQTRLKVNFSDDSDLEDLVS AETQLVEEPKRRGTASRTRGQSRKGPSLKTDAAVAVGSTPGHPSLSGRTRRARKVASRHCEGVGASLGPE IMRSIPEEELMDSQLEKSFEILRASDGEDSASGEKAAAAEPGLPIGEREVLRRDSSKAERPVLYPDTEAD SDLSPWLPPFSIPAPIDLSTLDSIADSLSIAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVAES VSITCRHQLLTHLHRQLSKAQKQQGSPELAEHLQRLDLKERPGGVPLARVQHLFSFRALGSGCFPQAEKD SFQKRLALIPSGVTVCVLALATLQPGTLSNTLLLTRLEKDNPPITVKIPTAQSKFPLSAVLKEFDAIQKD QKENSGCTEKRAWWTGRLALDQRMEELITSLEEHVLGCWRGLLLPCSTDPGLSQEASQLQELLHECGWEY PDYTLLKVILSGARILSSQDVQALACGLCPAQPERAQVLLSEAVGRVQSQAAPPSQHLVLVLDKDLQKLP WESMPCLRALPVTRLPSLRFLLSYSIAKEAGASSVLSQGVDPRNTFYVLNPHSNLSSTEERFRASFSSEA GWKGVIGEVPSLEQVQAALTEHDLYIYAGHGAGARFLDGQAVLRLNCRAVALLFGCSSAALAVHGNLEGA GIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLHYVSQARQAPRLKYLIGAAPVAY GLPVSLQTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001170602
ORF Size	6327 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001170602.1, NP_001164073.1
RefSeq Size	6835 bp
RefSeq ORF	6330 bp
Locus ID	315330
MW	231.6 kDa

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