NEDD4 2 (NEDD4L) (NM_015277) Human Untagged Clone
CAT＃: SC102592
NEDD4L (untagged)-Human neural precursor cell expressed, developmentally down-regulated 4-like (NEDD4L), transcript variant d
CNY 7000.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hNEDD4-2; NEDD4-2; NEDD4.2; PVNH7; RSP5 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC102592 sequence for NM_015277 edited (data generated by NextGen Sequencing)
ATGGCGACCGGGCTCGGGGAGCCGGTCTATGGACTTTCCGAAGACGAGGGAGAGTCCCGT ATTCTCAGAGTAAAAGTTGTTTCTGGAATTGATCTCGCCAAAAAGGACATCTTTGGAGCC AGTGATCCGTATGTGAAACTTTCATTGTACGTAGCGGATGAGAATAGAGAACTTGCTTTG GTCCAGACAAAAACAATTAAAAAGACACTGAACCCAAAATGGAATGAAGAATTTTATTTC AGGGTAAACCCATCTAATCACAGACTCCTATTTGAAGTATTTGACGAAAATAGACTGACA CGAGACGACTTCCTGGGCCAGGTGGACGTGCCCCTTAGTCACCTTCCGACAGAAGATCCA ACCATGGAGCGACCCTATACATTTAAGGACTTTCTCCTCAGACCAAGAAGTCATAAGTCT CGAGTTAAGGGATTTTTGCGATTGAAAATGGCCTATATGCCAAAAAATGGAGGTCAAGAT GAAGAAAACAGTGACCAGAGGGATGACATGGAGCATGGATGGGAAGTTGTTGACTCAAAT GACTCGGCTTCTCAGCACCAAGAGGAACTTCCTCCTCCTCCTCTGCCTCCCGGGTGGGAA GAAAAAGTGGACAATTTAGGCCGAACTTACTATGTCAACCACAACAACCGGACCACTCAG TGGCACAGACCAAGCCTGATGGACGTGTCCTCGGAGTCGGACAATAACATCAGACAGATC AACCAGGAGGCAGCACACCGGCGCTTCCGCTCCCGCAGGCACATCAGCGAAGACTTGGAG CCCGAGCCCTCGGAGGGCGGGGATGTCCCCGAGCCTTGGGAGACCATTTCAGAGGAAGTG AATATCGCTGGAGACTCTCTCGGTCTGGCTCTGCCCCCACCACCGGCCTCCCCAGGATCT CGGACCAGCCCTCAGGAGCTGTCAGAGGAACTAAGCAGAAGGCTTCAGATCACTCCAGAC TCCAATGGGGAACAGTTCAGCTCTTTGATTCAAAGAGAACCCTCCTCAAGGTTGAGGTCA TGCAGTGTCACCGACGCAGTTGCAGAACAGGGCCATCTACCACCGCCATCAGTGGCCTAT GTACATACCACGCCGGGTCTGCCTTCAGGCTGGGAAGAAAGAAAAGATGCTAAGGGGCGC ACATACTATGTCAATCATAACAATCGAACCACAACTTGGACTCGACCTATCATGCAGCTT GCAGAAGATGGTGCGTCCGGATCAGCCACAAACAGTAACAACCATCTAATCGAGCCTCAG ATCCGCCGGCCTCGTAGCCTCAGCTCGCCAACAGTAACTTTATCTGCCCCGCTGGAGGGT GCCAAGGACTCACCCGTACGTCGGGCTGTGAAAGACACCCTTTCCAACCCACAGTCCCCA CAGCCATCACCTTACAACTCCCCCAAACCACAACACAAAGTCACACAGAGCTTCTTGCCA CCCGGCTGGGAAATGAGGATAGCGCCAAACGGCCGGCCCTTCTTCATTGATCATAACACA AAGACTACAACCTGGGAAGATCCACGTTTGAAATTTCCAGTACATATGCGGTCAAAGACA TCTTTAAACCCCAATGACCTTGGCCCCCTTCCTCCTGGCTGGGAAGAAAGAATTCACTTG GATGGCCGAACGTTTTATATTGATCATAATAGCAAAATTACTCAGTGGGAAGACCCAAGA CTGCAGAACCCAGCTATTACTGGTCCGGCTGTCCCTTACTCCAGAGAATTTAAGCAGAAA TATGACTACTTCAGGAAGAAATTAAAGAAACCTGCTGATATCCCCAATAGGTTTGAAATG AAACTTCACAGAAATAACATATTTGAAGAGTCCTATCGGAGAATTATGTCCGTGAAAAGA CCAGATGTCCTAAAAGCTAGACTGTGGATTGAGTTTGAATCAGAGAAAGGTCTTGACTAT GGGGGTGTGGCCAGAGAATGGTTCTTCTTACTGTCCAAAGAGATGTTCAACCCCTACTAC GGCCTCTTTGAGTACTCTGCCACGGACAACTACACCCTTCAGATCAACCCTAATTCAGGC CTCTGTAATGAGGATCATTTGTCCTACTTCACTTTTATTGGAAGAGTTGCTGGTCTGGCC GTATTTCATGGGAAGCTCTTAGATGGTTTCTTCATTAGACCATTTTACAAGATGATGTTG GGAAAGCAGATAACCCTGAATGACATGGAATCTGTGGATAGTGAATATTACAACTCTTTG AAATGGATCCTGGAGAATGACCCTACTGAGCTGGACCTCATGTTCTGCATAGACGAAGAA AACTTTGGACAGACATATCAAGTGGATTTGAAGCCCAATGGGTCAGAAATAATGGTCACA AATGAAAACAAAAGGGAATATATCGACTTAGTCATCCAGTGGAGATTTGTGAACAGGGTC CAGAAGCAGATGAACGCCTTCTTGGAGGGATTCACAGAACTACTTCCTATTGATTTGATT AAAATTTTTGATGAAAATGAGCTGGAGTTGCTCATGTGCGGCCTCGGTGATGTGGATGTG AATGACTGGAGACAGCATTCTATTTACAAGAACGGCTACTGCCCAAACCACCCCGTCATT CAGTGGTTCTGGAAGGCTGTGCTACTCATGGACGCCGAAAAGCGTATCCGGTTACTGCAG TTTGTCACAGGGACATCGCGAGTACCTATGAATGGATTTGCCGAACTTTATGGTTCCAAT GGTCCTCAGCTGTTTACAATAGAGCAATGGGGCAGTCCTGAGAAACTGCCCAGAGCTCAC ACATGCTTTAATCGCCTTGACTTACCTCCATATGAAACCTTTGAAGATTTACGAGAGAAA CTTCTCATGGCCGTGGAAAATGCTCAAGGATTTGAAGGGGTGGATTAA Clone variation with respect to NM_015277.5 >OriGene 5' read for NM_015277 unedited
TTCGGCACCAGGGAGAACCGGCCGTCCGCGCCGCAGCACAGCCGCTGGGAGCGCCTCAGA CCCCGCGCGGGGCGCCGGCTCCATGGCGACCGGGCTCGGGGAGCCGGTCTATGGACTTTC CGAAGACGAGGGAGAGTCCCGTATTCTCAGAGTAAAAGTTGTTTCTGGAATTGATCTCGC CAAAAAGGACATCTTTGGAGCCAGTGATCCGTATGTGAAACTTTCATTGTACGTAGCGGA TGAGAATAGAGAACTTGCTTTGGTCCAGACAAAAACAATTAAAAAGACACTGAACCCAAA ATGGAATGAAGAATTTTATTTCAGGGTAAACCCATCTAATCACAGACTCCTATTTGAAGT ATTTGACGAAAATAGACTGACACGAGACGACTTCCTGGGCCAGGTGGACGTGCCCCTTAG TCACCTTCCGACAGAAGATCCAACCATGGAGCGACCCTATACATTTAAGGACTTTCTCCT CAGACCAAGAAGTCATAAGTCTCGAGTTAAGGGATTTTTGCGATTGAAAATGGCCTATAT GCCAAAAAATGGAGGTCAAGATGAAGAAAACAGTGACCAGAGGGATGACATGGAGCATGG ATGGGAAGTTGTTGACTCAAATGACTCGGCTTCTCAGCACCAAGAGGAACTTCCTCCTCC TTCTCTGCCTCCCGGGTGGGAAGAAAAAGTGGACAATNTANGCCGAACTTACTATGTCAA CCACAACAACCGGACCACTCAGTGGCACAGACCAAGCCTGATGGACGTGT >OriGene 3' read for NM_015277 unedited
NNTTTTACTCTGAACCGCGGCCGCATCTAAGATCGGTTTTTTTTTTTTTTTTTTTAGTTA CATAGCATCTAAGCTTTTCTGATCACTACCAGGTAATTTTCAACCAATAAGAAAAAGGAA CCAACACTCAGCTCTGTAGAAATCTACCTTCTTTTAGAAACCTCAGGCCTCTGCACCCCT TTAGACAACTCATTTCCAACCACAACCTTCCATGGCTTCTCACATGCCAGCCAGGGCAGG GTAAAAACGGCAATTCCTTACACCGAAAGGTCCTTTTTATGGAACAAACAGACCTTCCCA AACCACAACTTTTTCGGCCTATTGATGACTCGCATGAGATCAAATCTCTAGAGAACCCTG CACCGCCCCCACAGGGCCGTGGGATGTCTCACGATCACAGAAGCGTCCCTGGCCCCCCCT TCCTTCCACTCCGACTTTACGTTTCTCCCCCTAATCCCCCGCCCGCGCCCCCCCCGGCCC CCCGCCCCCACATACCCCCTCTTCCCCCCGCCCCCCCCCCTCCCCCCTCCCGCCCCCCCC CCCCCCCCCCTCTCCCGCCCCTCCCTCCCCCCCCTTGCCCCTCCCCCTCACACCGCCCCC CCACGTTCCCTCCCGCCCCCACCGTCCTCTCCCCACCCCCCCCACCGCTCACCCGCATCT ACCCTCCTCTCACCTCTATCCCCGTTCCTCCTAGCCCCCCCGACAGCTCTCCTATGCTCT TTTTTGCCCCCCTCGCGCCCACGCCGCTCCACCACCAACCCGCACCCCACTTGCCGACTG CCACCAACATCTCCCCCACCTGACGCCACCCTTCAAAGCCCCGCCCCTCCCCCCTCCGAC CCACGCCGCCTCCGCGCCTCCCTCTCCCNCCCCTCCACACCTCAACCCCCCCCCCACCCC CCTACCCCGCCCCCACCGTCCCTCACGCCCGCCACCCCACTCCCTCGCTCTCTTCTCACC CGCTCTCCCCACCCCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_015277 |
| Insert Size | 5000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015277.2, NP_056092.2 |
| RefSeq Size | 4871 bp |
| RefSeq ORF | 2868 bp |
| Locus ID | 23327 |
| UniProt ID | Q96PU5 |
| Domains | C2, HECT, WW |
| Protein Families | Druggable Genome |
| Protein Pathways | Endocytosis, Ubiquitin mediated proteolysis |
| Gene Summary | This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (d) lacks an in-frame exon, compared to variant j, resulting in a shorter protein (isoform 3), compared to isoform 1. Variant d is also known as NEDD4-2C6 and NEDL3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217897 | NEDD4L (Myc-DDK-tagged)-Human neural precursor cell expressed, developmentally down-regulated 4-like (NEDD4L), transcript variant d |
CNY 9790.00 |
|
| RC217897L3 | Lenti-ORF clone of NEDD4L (Myc-DDK-tagged)-Human neural precursor cell expressed, developmentally down-regulated 4-like (NEDD4L), transcript variant d |
CNY 11690.00 |
|
| RC217897L4 | Lenti-ORF clone of NEDD4L (mGFP-tagged)-Human neural precursor cell expressed, developmentally down-regulated 4-like (NEDD4L), transcript variant d |
CNY 11690.00 |
|
| RG217897 | NEDD4L (tGFP-tagged) - Human neural precursor cell expressed, developmentally down-regulated 4-like (NEDD4L), transcript variant d |
CNY 10740.00 |
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