RPUSD4 (NM_032795) Human Untagged Clone
CAT＃: SC103625
RPUSD4 (untagged)-Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1
CNY 3656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_032795, the custom clone sequence may differ by one or more nucleotides
ATGGCGGCGCCCAGGTGGAGCGCGTCGGGCCCCTGGATCCGGGGAAACGGCCAAGGTTGCGGGAGTCTCT TCACTCTCGTCTCAAAGCCATTTTGTGCCGCTGCCGCTGCCTCTACGGCCATAAATGCCCAGAGATTAGC GGAGAAGCTCCGAGCCCAGAAACGGGAACAAGACACAAAGAAGGAGCCGGTGTCCACAAACGCTGTTCAG CGGAGAGTGCAAGAAATAGTGCGGTTCACACGGCAGCTGCAGCGAGTCCACCCCAACGTGCTTGCTAAGG CACTGACCCGAGGAATTCTCCACCAGGACAAGAACCTTGTGGTCATCAATAAGCCCTACGGTCTCCCTGT GCATGGTGGCCCTGGGGTCCAGCTCTGCATCACTGATGTACTACCTATCCTGGCAAAGATGCTTCATGGC CACAAGGCAGAGCCCTTGCATCTGTGCCACCGGCTGGACAAGGAAACCACAGGTGTAATGGTGTTGGCTT GGGACAAGGACATGGCACATCAAGTCCAAGAGTTGTTTAGAACCCGTCAGGTGGTGAAGAAGTACTGGGC CATCACTGTGCATGTCCCCATGCCCTCAGCAGGAGTCGTGGACATCCCCATTGTGGAGAAGGAGGCGCAA GGCCAGCAGCAACACCACAAGATGACATTGTCCCCGAGCTACCGCATGGACGATGGGAAAATGGTGAAAG TGCGGCGCAGCCGGAATGCGCAAGTTGCTGTAACTCAGTACCAGGTGCTCAGCAGCACTCTCTCCTCCGC CCTCGTGGAGCTCCAGCCCATCACTGGAATAAAACATCAGCTTCGAGTTCACTTGTCTTTTGGATTGGAT TGTCCAATCCTTGGTGATCACAAGTACTCAGACTGGAATAGGTTGGCCCCCCAGAAGCTGTCTGTGGGCA CCCTGAAGAAGCTGGGGCTAGAACAGTCGAAGGCCCGCTACATCCCCCTTCACCTGCACGCCCGGCAGCT GATCCTGCCTGCCCTGGGGTCCGGGAAGGAGGAACTCAACTTGGTCTGCAAACTTCCTCGCTTCTTTGTG CATTCCCTGCACCGCCTGCGTTTAGAGATGCCAAATGAGGATCAAAATGAGAACAATGAAGCCAAGTGTC TGGGAGCACAGTGA >OriGene 5' read for NM_032795 unedited
AGTATTTTGTAATACGACTCACTATTAGGGCGGCCGCGATTCGGCACGAGGCCCGCCCTT CCTTGTAAGATGGCGGCGCCCAGGTGGAGCGCGTCGGGCCCCTGGATCCGGGGAAACGGC CAAGGTTGCGGGAGTCTCTTCACTCTCGTCTCAAAGCCATTTTGTGCCGCTGCCGCTGCC TCTACGGCCATAAATGCCCGGAGATTAGCGGAGAAGCTCCGAGCCCAGAAACGGGAACAA GACACAAAGAAGGAGCCGGTGTCCACAAACGCTGTTCAGCGGAGAGTGCAAGAAATAGTG CGGTTCACACGGCAGCTGCAGCGAGTCCACCCCAACGTGCTTGCTAAGGCACTGACCCGA GGAATTCTCCACCAGGACAAGAACCTTGTGGTCATCAATAAGCCCTACGGTCTCCCTGTG CATGGTGGCCCTGGGGTCCAGCTCTGCATCACTGATGTACTACCTATCCTGGCAAAGATG CTTCATGGCCACAAGGCAGAGCCCTTGCATCTGTGCCACCGGCTGGACAAGGAAACCACA GGTGTAATGGTGTTGGCTTGGGACAAGGACATGGCACATCAAGTCCAAGAGTTGTTTAGA ACCCGTCAGGTGGTGAAGAAGTACTGGGCCATCACTGTGCATGTCCCCATGCCCTCAGCA GGAGTCGTGGACATCCCCATTGTGGAGAAGGAGGCGCAAGGCCAGCAGCAACACCACAAG ATGACATTGTCCCCGAGCTACCGCATGGACGATGGGAAAATGGTGAAAGTGCGGCGCAGC CCGAATGCGCAAGNTGCTGTAACTCAGTACCAGGTGCTCAGCAGCACTCTCTCCTNCGNC CTCGTGGAGCTCCAGCCCATCACTGGNNATANACATCAGCTTCGAGTCACTTGTCCTTTG NATGGNATNGNNCATNNCTNGTGATCACAGTACTCAGACTGGAATAGGTGGNCC >OriGene 3' read for NM_032795 unedited
TTTGGACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTGCTTTCAAGTT AAATTTATTATGCACAGTAATGGGTACCATGGAGACAGGGAAAGGGCTAGAAAGTGGCAT CATCTATACAGAGGGAACATATAAAGACTGTCCCGGGGTGTACCCCCCTAACTGTGCTGT CACTCCAACTCTGTCAGAAAGAAAAAGTAGTGTGAGGTGTATTTGTTTGCAAGAAACAAG GTGGAAACTAGATGGACCAGCTGAGCAGGGAGGCCAAGGTCATTTCCATCTATCCCTAAT GGTGTTCAGTATAGCCCTCTCCTTCCTGAGACCTAAGAACAGAAGATACGCTTCCACAAC AGAGATTTTTGATTTTTTAATGGCAGCACTCTCATAACTACATTCACGGACCAACCAAAG CCATCTCCAAGCCTAGAAAGGATACATACTACACGCAGCGAAAATGCCAACCACTGATCC AGAATTTTCAAGATGGCACCTTTCCCCAAACTTCTATACCATTCATTAAACATAGACTGC AAACTGCTAGCCTGTAGACCAAATTCAACCTCTAAGTATTATTTGCTTAGGTGCCTAAGC ATCTTTAAAAATTTGATTTAGTTGGCAATACACTGGGAGGTTTCACATAAATATCTAGGT TTCCAGCTTCTCTGAAGAAATGAGAAAATCCATTTCCACATGGGCACAACCAGCTGGAGC TAACAGCTGTTAGACAGCATGCTGCTCCCCGCTACTGGCTTACACCCAGCCAGCTTCATG TGTGCATGTTATCTGCCTGCCATCACATCGGTCTCCCTGCTCAGCAATGTCCCACCTCAC CTCACACTCATACCCTTATACAGTTCCCTAATCAGATTCAGTAGGCCACCCCACTATAGC TNGGCATCCCTGGAGCTGGAAATTTGTAACATNTGNTCTTTCACCACGGACCTNCTAGAG TCAGACTCCTGTGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_032795 |
| Insert Size | 2640 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032795.1, NP_116184.1 |
| RefSeq Size | 2146 bp |
| RefSeq ORF | 1134 bp |
| Locus ID | 84881 |
| UniProt ID | Q96CM3 |
| Domains | PseudoU_synth_2 |
| Gene Summary | Catalyzes uridine to pseudouridine isomerization (pseudouridylation) of different mitochondrial RNA substrates. Acts on position 1397 in 16S mitochondrial ribosomal RNA (16S mt-rRNA). This modification is required for the assembly of 16S mt-rRNA into a functional mitochondrial ribosome (PubMed:27974379). Acts on position 39 in mitochondrial tRNA(Phe) (PubMed:28082677). As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mt-rRNA, controls 16S mt-rRNA abundance and is required for intra-mitochondrial translation (PubMed:27667664).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204352 | RPUSD4 (Myc-DDK-tagged)-Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1 |
CNY 3656.00 |
|
| RC204352L3 | Lenti ORF clone of Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC204352L4 | Lenti ORF clone of Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG204352 | RPUSD4 (tGFP-tagged) - Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1 |
CNY 5256.00 |
