C20orf7 (NDUFAF5) (AK091060) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA526K24.2; C20orf7; dJ842G6.1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF sequence for AK091060 edited ATGCTGCGGCCGGCAGGGCTCTGGCGCTTATGTCGGCGACCTTGGGCGGCGAGGGTCCCA GCGGAGAATCTTGGCCGTAGGGAAGTCACCTCTGGTGTCTCTCCCCGCGGTAGCACCTCG CCCAGAACCCTGAATATTTTCGACCGGGATTTGAAAAGGAAACAGAAGAACTGGGCAGCC CGGCAGCCCGAGCCGACCAAATTTGACTACCTGAAGGAGGAGGTTGGAAGTCGGATCGCA GACCGTGTATATGACATACCCAGAAATTTCCCCCTTGCTTTGGATCTTGGTTGTGGAAGA GGTTACATTGCACAATATTTGAATAAGGAAACTATTGGAAAGTTTTTCCAAGCTGACATT GCAGAAAATGCTTTGAAAAATTCCTCAGAAACAGAAATACCTACTGTCAGCGTTTTAGCT GATGAAGAATTCCTTCCCTTCAAAGAAAATACATTTGACCTGGTGGTTAGCAGTTTAAGT TTGCATTGGGTGAATGACCTTCCTAGAGCACTTGAGCAGATTCATTATATTTTAAAACCA GATGGAGTGTTTATCGGTGCAATGTTTGGAGGCGACACACTCTATGAACTTCGGTGTTCC TTACAGTTAGCGGAAACGGAAAGGGAAGGAGGATTTTCTCCACACATTTCTCCTTTCACT GCTGTCAATGACCTGGGACATCTGCTTGGGAGAGCTGGCTTTAATACTCTGACTGTGGAC ACTGATGAAATTCAAGTTAACTATCCTGGAATGTTTGAATTGATGGAAGATTTACAAGGT ATGGGTGAGAGTAACTGTGCTTGGAATAGAAAAGCCCTGCTGCATCGAGACACAATGCTG GCAGCTGCGGCAGTGTACAGAGAAATGTACAGAAATGAAGATGGTTCAGTACCTGCTACA TACCAGATCTATTACATGATAGGATGGAAATATCATGAGTCACAGGCAAGACCAGCTGAA AGAGGTTCCGCAACTGTGTCATTTGGAGAGCTAGGAAAAATAAACAACCTTATGCCACCG GGGAAAAAATCACAATAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK091060 unedited TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGTCGCAGCTGGAGAT GCTGCGGCCGGCAGGGCTCTGGCGCTTATGTCGGCGACCTTGGGCGGCGAGGGTCCCAGC GGAGAATCTTGGCCGTAGGGAAGTCACCTCTGGTGTCTCTCCCCGCGGTAGCACCTCGCC CAGAACCCTGAATATTTTCGACCGGGATTTGAAAAGGAAACAGAAGAACTGGGCAGCCCG GCAGCCCGAGCCGACCAAATTTGACTACCTGAAGGAGGAGGTTGGAAGTCGGATCGCAGA CCGTGTATATGACATACCCAGAAATTTCCCCCTTGCTTTGGATCTTGGTTGTGGAAGAGG TTACATTGCACAATATTTGAATAAGGAAACTATTGGAAAGTTTTTCCAAGCTGACATTGC AGAAAATGCTTTGAAAAATTCCTCAGAAACAGAAATACCTACTGTCAGCGTTTTAGCTGA TGAAGAATTCCTTCCCTTCAAAGAAAATACATTTGACCTGGTGGTTAGCAGTTTAAGTTT GCATTGGGTGAATGACCTTCCTAGAGCACTTGAGCAGATTCATTATATTTTAAAACCAGA TGGAGTGTTTATCGGTGCAATGTTTGGAGGCGACACACTCTATGAACTTCGGTGTTCCTT ACAGTTAGCGGAAACGGATAGGGAAGGAGGATTTTCTCCACACATTTCTCCTTTCACTGC TGTCAATGACCTGAGACATCTGCTTGGGAGAGCTGGCCTTATACTCTGACTGTGGACACT GATGAAATTCAAGTTACTATCCTGGAATGTTTGAATTGATGGAAGATTTACCAGTATGGG TGAGAGTTACTGTGCTGGNAATAGAAAAGCCCTGCTGCATCGAAACACATGCTNGCAGCT GCGGCAGTGTACANAGTAATGTACAAAATGAAGATTCCTCAGCCN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK091060 unedited GTACCGCGGCCGCTTTTAGNATCGAGTTTTTTTTTTTTTTTTTTAACACTGGAAAAATAT TTATTGTGATTTTTTCCCCGGTGGCATAAGGTTGTTTATTTTTCCTAGCTCTCCAAATGA CACAGTTGCGGAACCTCTTTCAGCTGGTCTTGCCTGTGACTCATGATATTTCCATCCTAT CATGTAATAGATCTGGTATGTAGCAGGTACTGAACCATCTTCATTTCTGTACATTTCTCT GTACACTGCCGCAGCTGCCAGCATTGTGTCTCGATGCAGCAGGGCTTTTCTATTCCAAGC ACAGTTACTCTCACCCATACCTTGTAAATCTTCCATCAATTCAAACATTCCAGGATAGTT AACTTGAATTTCATCAGTGTCCACAGTCAGAGTATTAAAGCCAGCTCTCCCAAGCAGATG TCCCAGGTCATTGACAGCAGTGAAAGGAGAAATGTGTGGAGAAAATCCTCCTTCCCTTTC CGTTTCCGCTAACTGTAAGGAACACCGAAGTTCATAGAGTGTGTCGCCTCCAAACATTGC ACCGATAAACACTCCATCTGGTTTTAAAATATAATGAATCTGCTCAAGTGCTCTAGGAAG GTCATTCACCCAATGCAAACTTAAACTGCTAACCACCAGGTCAAATGTATTTTCTTTGAA GGGAAGGAATTCTTCATCAGCTAAAACGCTGACAGTAGGTATTTCTGTTTCTGAGGAATT TTTCAAAGCATTNTCTGCAATGTCAGCTTGGAAAAACTTTCCAATAGTTTCCTTATTCAA ATATTGTGCAATGTAACCTCTTTCCACACCAAGATCCAAAGCAAGGGGGAAATTNCTGGG TATGTCATATACACGGTCTGCGATCCGACTTCCACCTCCTCCTCCAGTAGTCAATTTTAG TCGCTCGGCTGCAGGCTGCCCAGTTCTCA
Restriction Sites	NotI-NotI
ACCN	AK091060
Insert Size	1020 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK091060.1, BAG52273.1
RefSeq Size	2341 bp
RefSeq ORF	804 bp
Locus ID	79133
Protein Families	Druggable Genome
Gene Summary	The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes a mitochondrial protein that is associated with the matrix face of the mitochondrial inner membrane and is required for complex I assembly. A mutation in this gene results in mitochondrial complex I deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.