BC011938 Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC011938, the custom clone sequence may differ by one or more nucleotides GTGATGAGGACCAGCAAGAATTTGCAACAGGAACGCAGCTTCCATAGCAAAGTCAAGGGGAGGGGAGCTG CCGCCCTGGGCTTGCCTGGCAGGAATTAGCTTATGTACCAAATTGTTTGTGACAGTGCTGAGCAGGAGAC GCTGGCTTGTGAGGAGGAAGGCTTTTTTAAACAATTTGGTTAAAATGTTCAAATTGCCAGCTCTGACTCT TGCCCTGGAGAGGAGGGCAGCGGCCTGCTGTTGACTCCCTGATGGCTGGAGCAGTGGAAGCCACTAAGAA TGGCTAAAGATCACCCAAGCTACGGGCAAGGGCAATCTCGTGGGTCCGCAGCCCAAGGCAGAGAGAGACA TGGAGTTTACCACCTCCCCGGCAGCTCCTGCCACTGCCCAGCGTCTTGATGAAACAGTATGGAAACACGG CTGTCATTTATCCAGGTGTCTGCCTAGCAGGTACAGGAATGTGGGCTTGGGGACTGGAGCCCCCACCTTA AAAAGAGGTGAGGCAATGGAAAGGACCAGAGGGGACCTGATTCAGCAATTTACAGTGCCTTGGAGCTCGC CAGCAGCACCTCATTTGCATCTGGATTCCAGCCCTGGCATCTGCCTCGCCCCGCTCTGCTCACAAAGTAA CCCCACTGTCTTTCCACAAAGCCAGGCACTCCTTAGCCTAACGGCAGATCCTAGCCCTGAGTGCCCAGAA ATTCTATGTAAAGAATGAGAACCAAACCAGGCTCCCACTAATTTAGAATTCAAACAACCCCAAAGCTAAA ATAACCCCAATTTTTTTCTATATTGCATAGTCATCAGTGAGCTTTATAATTTTGTCCTAGAAACCCCCCC AGAGTCCCTAAGTGCCTTTGGCCTATCAAAGTAAGACTCATTTATGTTCAGTCTAGTTTGTGTTAAATGT GTTTTGTGCTATCCTGATTCCTTTAAAAATGGTAATTCATTCATGAGAAATACGATAAAAAAAAAAAAAA AAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC011938 unedited GGTGTGCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCAATCT CGTGGGTCCGCAGCCCAAGGCAGAGAGAGACATGGAGTTTACCACCTCCCCGGCAGCTCC TGCCACTGCCCAGCGTCTTGATGAAACAGTATGGAAACACGGCTGTCATTTATCCAGGTG TCTGCCTAGCAGGTACAGGAATGTGGGCTTGGGGACTGGAGCCCCCACCTTAAAAAGAGG TGAGGCAATGGAAAGGACCAGAGGGGCCCTGATTCAGCAATTTACAGTGCCTTGGAGCTC GCCAGCAGCACCTCATTTGCATCTGGATTCCAGCCCTGGCATCTGCCTCGCCCCGCTCTG CTCACAAAGTAACCCCACTGTCTTTCCACAAAGCCAGGCACTCCTTAGCCTAACGGCAGA TCCTAGCCCTGAGTGCCCAGAAATTCTATGTAAAGAATGAGAACCAAACCAGGCTCCCAC TAATTTAGAATTCAAACAACCCCAAAGCTAAAATAACCCCAATTTTTTTCTATATTGCAT AGTCATCAGTGAGCTTTATAATTTTGTCCTAGAAACCCCCCCAGAGTCCCTAAGTGCCTT TGGCCTATCAAAGTAAGACTCATTTATGTTCAGTCTAGTTTGTGTTAAATGTGTTTTGTG CTATCCTGATTCCTTTAAAAATGGTAATTCATTCATGAGAAATACGATAACTATTAACCA GACTGCCCCATTCCTGAACCATTTTGCATAATTGAACATGTATTCTATCAGCCTCTTCCT TTCCCCCAGGGATAGAGACCGACCTTTCTTTACATAACCNCTGNGCACTTGCCCCAGCCA GCAGGAAATCACTTAACCNCAGATGTTGAGGTTGAGGTGGGCCAGNATAGCGTCCCNCAC TTCAGCTGGATGACAGATGAGACACGTCTCAAAAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC011938 unedited TTGGACCGCGGCCGCTATCTAGGATCGAGTTTTTTTTTTTTTTTTTTATTTATTTATTTT TTGAGACGGTGTCTCACTCTGTCATCCAAGCTGGAGTGTGGGGACGCTATCTTGGCCCAC CTCAACCTCCACATCCTGGGTTTAAGTGATTCTCCTGCTGGCTTGGGCAAGTGCCCAGGG GTTATGTAAAGAAAAGTCGGTCTCTATCCCTGGGGGGAAAGGAAGAGGCTGATAGAATAC ATGTTCAATTATGCAAAATGGTTCCAGGAATGGGGGCCAGTCTGGTTAATAATTTATCGT ATTTCTCATGAATGAATTCCCATTTTTAAAGGAATCAGGATAGCACAAAACACATTTAAC ACAAACTAGACTGAACATAAATGAGTCTTACTTTGATAGGCCAAAGGCACTTAGGGACTC TGGGGGGGTTTCTAGGACAAAATTATAAAGCTCACTGATGACTATGCAATATAGAAAAAA ATTGGGGTTATTTTAGCTTTGGGGTTGTTTGAATTCTAAATTAGTGGGAGCCTGGTTTGG TTCTCATTCTTTACATAGAATTTCTGGGCACTCAGGGCTAGGATCTGCCGTTAGGCTAAG GAGTGCCTGGCTTTGTGGAAAGACAGTGGGGTTACTTTGTGAGCAGAGCGGGGCGAGGCA NATGCCAGGGCTGGGATCCAGATGCAAATGAGGTGCTGCTGGCGAGCTCCAGGCACTGTA ATTGCTGAACAGGGCCCTCTGGTCTTTCCATGCTACCTCTTTTAAGTGGGGCTCATTCCC AAGCCACTTCTGTCCTGCTAGCAGAACCTGNATAATGAAGCCTGTTTCAACTGTTTCTCA AACGCTGGCAATGCCAGACTCCGGGAAGTGTAAACTCTGTCTTTTCTGCCTGGCTGCGAA CCCCAATGGCCTCGTGCCATTCGGCCGCCTTAT
Restriction Sites	NotI-NotI
ACCN	BC011938
Insert Size	1000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC011938.1
RefSeq Size	993 bp
RefSeq ORF	822 bp

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