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ASAH1 (NM_004315) Human Untagged Clone
CAT＃: SC108952
ASAH1 (untagged)-Human N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 2
CNY 3,656.00
CNY 6,650.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AC; ACDase; ASAH; PHP; PHP32; SMAPME |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_004315, the custom clone sequence may differ by one or more nucleotides
ATGAACTGCTGCATCGGGCTGGGAGAGAAAGCTCGCGGGTCCCACCGGGCCTCCTACCCAAGTCTCAGCG CGCTTTTCACCGAGGCCTCAATTCTGGGATTTGGCAGCTTTGCTGTGAAAGCCCAATGGACAGAGGACTG CAGAAAATCAACCTATCCTCCTTCAGGACCAACGTACAGAGGTGCAGTTCCATGGTACACCATAAATCTT GACTTACCACCCTACAAAAGATGGCATGAATTGATGCTTGACAAGGCACCAGTGCTAAAGGTTATAGTGA ATTCTCTGAAGAATATGATAAATACATTCGTGCCAAGTGGAAAAATTATGCAGGTGGTGGATGAAAAATT GCCTGGCCTACTTGGCAACTTTCCTGGCCCTTTTGAAGAGGAAATGAAGGGTATTGCCGCTGTTACTGAT ATACCTTTAGGAGAGATTATTTCATTCAATATTTTTTATGAATTATTTACCATTTGTACTTCAATAGTAG CAGAAGACAAAAAAGGTCATCTAATACATGGGAGAAACATGGATTTTGGAGTATTTCTTGGGTGGAACAT AAATAATGATACCTGGGTCATAACTGAGCAACTAAAACCTTTAACAGTGAATTTGGATTTCCAAAGAAAC AACAAAACTGTCTTCAAGGCTTCAAGCTTTGCTGGCTATGTGGGCATGTTAACAGGATTCAAACCAGGAC TGTTCAGTCTTACACTGAATGAACGTTTCAGTATAAATGGTGGTTATCTGGGTATTCTAGAATGGATTCT GGGAAAGAAAGATGTCATGTGGATAGGGTTCCTCACTAGAACAGTTCTGGAAAATAGCACAAGTTATGAA GAAGCCAAGAATTTATTGACCAAGACCAAGATATTGGCCCCAGCCTACTTTATCCTGGGAGGCAACCAGT CTGGGGAAGGTTGTGTGATTACACGAGACAGAAAGGAATCATTGGATGTATATGAACTCGATGCTAAGCA GGGTAGATGGTATGTGGTACAAACAAATTATGACCGTTGGAAACATCCCTTCTTCCTTGATGATCGCAGA ACGCCTGCAAAGATGTGTCTGAACCGCACCAGCCAAGAGAATATCTCATTTGAAACCATGTATGATGTCC TGTCAACAAAACCTGTCCTCAACAAGCTGACCGTATACACAACCTTGATAGATGTTACCAAAGGTCAATT CGAAACTTACCTGCGGGACTGCCCTGACCCTTGTATAGGTTGGTGA >OriGene 5' read for NM_004315 unedited
ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCACGGATGGCAC GAAAGCCAAGCGAGTCCCCCTGCCGAGCTACTCGCGTCCGCCTCCTCCCAAGCTGAGCTC TGCTCCGCCCACCTGAGTCCTTCGCCAGTTAGGAGGAAACACAGCCGCTTAATGAACTGC TGCATCGGGCTGGGAGAGAAAGCTCGCGGGTCCCACCGGGCCTCCTACCCAAGTCTCAGC GCGCTTTTCACCGAGGCCTCAATTCTGGGATTTGGCAGCTTTGCTGTGAAAGCCCAATGG ACAGAGGACTGCAGAAAATCAACCTATCCTCCTTCAGGACCAACGTACAGAGGTGCAGTT CCATGGTACACCATAAATCTTGACTTACCACCCTACAAAAGATGGCATGAATTGATGCTT GACAAGGCACCAATGCTAAAGGTTATAGTGAATTCTCTGAAGAATATGATAAATACATTC GTGCCAAGTGGAAAAGTTATGCAGGTGGTGGATGAAAAATTGCCTGGCCTACTTGGCAAC TTTCCTGGCCCTTTTGAAGAGGAAATGAAGGGTATTGCCGCTGTTACTGATATACCTTTA GGAGAGATTATTTCATTCAATATTTTTTATGAATTATTTACCATTTTGTACTTCAATAGT AGCAGAAGAAAAAAAGGTCATCTAATACATGGGAGAAACATGGNATTTGGAGTATTTCTT GGGTGGAACATANATAATGATACCCTGNGTCATAACTGAGCAAACTAAACCTTTACAGTG AATNTGGATTNCAAAAGAAACACANNACTGTCTCAGGCTTCAAGCTTTGCTGCTATGNGG GCATGTTACAGGATTTCAACAGACTGTTCAGTCTAACTGAATGAAA >OriGene 3' read for NM_004315 unedited
GCTCTTGCTGTCTGTTTCCTGAGTTGTCAGGGAACACAACTGTGCATTATACAAAATGAA GTGGACAAACGGTCTTGCACAAATGACGTACATTTCACAGTTAATCGTGAAGGTTAGAAA GGTTAACAAGTTCTATTAGTACTTTTCATAAGCAGTTGAGATTTCTAGAAAAATACAGTA ACATAATTTTAGTAACTACTAAAATTTCTTTAAAACTCCCTATCAATCTGAAGAACAAAC TAAAAACCTGTTTATTACATGAATGCTACTTATGAGAATTTAAAATATGGGTTCACTTCC TATTTTCCACAAGTCTTTGACTTGTTTATTTACTAAATTAACAGAACGTGGGATGCAGTT TTTTAAGTTAGCAACTTCCTTAGGGTTACTGGGAAGTAGAGTAATTATGAAAAAGGGAAC ACTTCAATTATTGTATAAAAGATTGCTTTGCATACTGATTACTTCTTGAACCCCAAAAAG TGCTATTTAGAGGCAAGGGCCCATTTTGTGCCCATTAAAAGAAATATACAGGTGAATGTC TGTCTCATCCCTCTAATCATATGTAATAAAATTATATCGCTGTCAATAACAGCAAAGATA ATGATATATTAATTTTAACAGCAGTTAGAACCAGAAGGAAAAGGCTGTTATACAGAAAAA CATACAAATATGTAAGTAGAAGTGAAAAACTGAAGAATGTGGAGTGTTTACTGTCCCGTT ACTCACACAGACGTGCTGGATTCAACACCCACGCTGAAATCTCATTTATTCCACGGGAGT GGAGTTCACCATGGTTCGACTGCCCACCANATTCCCCAATGTCAAAGTGAAACAAAACTC AGTGAATTCTAAATGACTGTTTTACTTCCCCTAAAGAAGTTATCTGGTAATTAGAAAAAA TCACTGATAGGGGGGAAAAAAAAGTA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004315 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004315.2, NP_004306.2 |
| RefSeq Size | 2503 bp |
| RefSeq ORF | 1236 bp |
| Locus ID | 427 |
| UniProt ID | Q13510 |
| Domains | CBAH |
| Protein Families | Druggable Genome |
| Protein Pathways | Lysosome, Metabolic pathways, Sphingolipid metabolism |
| Gene Summary | This gene encodes a member of the acid ceramidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. Processing of this preproprotein generates alpha and beta subunits that heterodimerize to form the mature lysosomal enzyme, which catalyzes the degradation of ceramide into sphingosine and free fatty acid. This enzyme is overexpressed in multiple human cancers and may play a role in cancer progression. Mutations in this gene are associated with the lysosomal storage disorder, Farber lipogranulomatosis, and a neuromuscular disorder, spinal muscular atrophy with progressive myoclonic epilepsy. [provided by RefSeq, Oct 2015] Transcript Variant: This variant (2) encodes the longest isoform (b). |
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212434 | ASAH1 (Myc-DDK-tagged)-Human N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 2 |
CNY 3,656.00 |
|
| RC212434L1 | Lenti ORF clone of Human N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212434L2 | Lenti ORF clone of Human N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC212434L3 | Lenti ORF clone of Human N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212434L4 | Lenti ORF clone of Human N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG212434 | ASAH1 (tGFP-tagged) - Human N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 2 |
CNY 4,370.00 |
