IFT122 (NM_018262) Human Untagged Clone
CAT＃: SC110525
IFT122 (untagged)-Human intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 3
CNY 8240.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CED; CED1; FAP80; SPG; WDR10; WDR10p; WDR140 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_018262, the custom clone sequence may differ by one or more nucleotides
ATGAGGGCCGTGTTGACGTGGAGAGATAAAGCCGAGCACTGTATAAATGACATCGCATTTAAGCCTGATG GAACTCAACTGATTTTGGCTGCCGGAAGCAGATTACTGGTTTATGACACCTCTGATGGCACCTTACTTCA GCCCCTCAAGGGACACAAAGACACTGTGTACTGTGTGGCATATGCGAAGGATGGCAAGCGCTTTGCTTCT GGATCAGCTGACAAAAGCGTTATTATCTGGACATCAAAACTGGAAGGCATTCTGAAGTACACGCACAATG ATGCTATACAATGTGTCTCCTACAATCCTATTACTCATCAACTGGCATCTTGTTCCTCCAGTGACTTTGG GTTGTGGTCTCCTGAACAGAAGTCTGTCTCCAAACACAAATCAAGCAGCAAGATCATCTGCTGCAGCTGG ACAAATGATGGTCAGTACCTGGCGCTGGGGATGTTCAATGGGATCATCAGCATACGGAACAAAAATGGCG AGGAGAAAGTAAAGATCGAGCGGCCGGGGGGCTCCCTCTCGCCAATATGGTCCATCTGCTGGAACCCTTC AAGAGAGGAACGTAATGACATCCTGGCTGTGGCTGACTGGGGACAGAAAGTTTCCTTCTACCAGCTGAGT GGAAAACAGATTGGAAAGGATCGGGCACTGAACTTTGACCCCTGCTGCATCAGCTACTTTACTAAAGGCG AGTACATTTTGCTGGGGGGTTCAGACAAGCAAGTATCTCTTTTCACCAAGGATGGAGTGCGGCTTGGGAC TGTTGGGGAGCAGAACTCCTGGGTGTGGACGTGTCAAGCGAAACCGGATTCCAACTATGTGGTGGTCGGC TGCCAGGACGGCACCATTTCCTTCTACCAGCTTATTTTCAGCACAGTCCATGGGCTTTACAAGGACCGCT ATGCCTACAGGGATAGCATGACTGACGTCATTGTGCAGCACCTGATCACTGAGCAGAAAGTTCGGATTAA ATGCAAAGAGCTTGTCAAGAAGATTGCCATCTACAGAAATCGATTGGCTATCCAACTGCCAGAGAAAATC CTCATCTATGAGTTGTATTCAGAGGACTTATCAGACATGCATTACCGGGTAAAGGAGAAGATTATCAAGA AGTTTGAGTGCAACCTCCTGGTGGTGTGTGCCAATCACATCATCCTGTGCCAGGAGAAACGGCTGCAGTG CCTGTCCTTCAGCGGAGTGAAGGAGCGGGAGTGGCAGATGGAGTCTCTCATTCGTTACATCAAGGTGATC GGTGGCCCTCCTGGAAGAGAAGGCCTCTTAGTGGGGCTGAAGAATGGACAGATCCTGAAGATCTTCGTGG ACAATCTCTTTGCTATCGTCCTGCTGAAGCAGGCCACAGCTGTGCGCTGCTTGGACATGAGTGCCTCCCG TAAGAAGCTGGCCGTGGTAGATGAAAATGACACTTGCCTGGTGTATGACATCGACACCAAGGAGCTGCTT TTTCAGGAACCAAACGCCAACAGTGTAGCTTGGAACACCCAGTGTGAGGACATGCTCTGCTTCTCGGGAG GAGGCTACCTCAACATCAAAGCCAGCACCTTCCCTGTGCACCGGCAGAAGCTGCAGGGCTTTGTGGTCGG CTACAATGGCTCCAAGATCTTCTGCCTCCATGTCTTCTCCATTTCTGCCGTGGAGGTGCCGCAGTCCGCT CCCATGTACCAGTACCTGGATAGGAAACTGTTCAAGGAAGCCTACCAGATTGCTTGCTTGGGTGTCACAG ACACTGATTGGCGTGAACTGGCCATGGAAGCGCTAGAAGGTTTAGATTTTGAAACAGCAAAGAAGGCCTT CATCAGAGTACAAGACCTCCGATATTTAGAGCTCATCAGCAGCATTGAGGAGAGGAAGAAGCGGGGAGAG ACCAACAATGACCTGTTTCTGGCAGATGTGTTTTCCTACCAGGGGAAGTTCCATGAGGCCGCCAAACTGT ACAAGAGGAGTGGGCACGAGAACCTCGCGCTTGAAATGTACACCGACCTCTGCATGTTTGAGTATGCCAA GGATTTCCTTGGATCTGGAGACCCCAAAGAAACAAAGATGCTAATCACCAAACAGGCTGACTGGGCCAGA AATATCAAGGAGCCCAAAGCCGCCGTGGAGATGTACATCTCAGCAGGAGAGCACGTCAAGGCCATCGAGA TCTGTGGTGACCATGGCTGGGTTGACATGTTGATCGACATCGCCCGCAAACTGGACAAGGCTGAGCGCGA GCCCCTGCTGCTGTGCGCTACCTACCTCAAGAAGCTGGACAGCCCTGGCTATGCTGCTGAGACCTACCTG AAGATGGGTGACCTCAAGTCCCTGGTGCAGCTGCACGTGGAGACCCAGCGCTGGGATGAGGCCTTTGCTT TGGGTGAGAAGCATCCTGAGTTTAAGGATGACATCTACATGCCGTATGCTCAGTGGCTAGCAGAGAACGA TCGCTTTGAGGAAGCCCAGAAAGCGTTCCACAAGGCTGGGCGACAGAGAGAAGCGGTCCAGGTGCTGGAG CAGCTCACAAACAATGCCGTGGCGGAGAGCAGGTTTAATGATGCTGCCTATTATTACTGGATGCTGTCCA TGCAGTGCCTCGATATAGCTCAAGATCCTGCCCAGAAGGACACAATGCTTGGCAAGTTCTACCACTTCCA GCGTTTGGCAGAGCTGTACCATGGTTACCATGCCATCCATCGCCACACGGAAGATCCGTTCAGTGTCCAT CGTCCTGAAACTCTTTTCAACATCTCCAGGTTCCTGCTGCACAGCCTGCCCAAGGACACCCCCTCGGGCA TCTCTAAAGTGAAAATACTCTTCACCTTGGCCAAGCAGAGCAAGGCCCTCGGTGCCTACAGGCTGGCCCG GCACGCCTATGACAAGCTGCGTGGCCTGTACATCCCTGCCAGATTCCAAAAGTCCATTGAGCTGGGTACC CTGACCATCCGCGCCAAGCCCTTCCACGACAGTGAGGAGTTGGTGCCCTTGTGCTACCGCTGCTCCACCA ACAACCCGCTGCTCAACAACCTGGGCAACGTCTGCATCAACTGCCGCCAGCCCTTCATCTTCTCCGCCTC TTCCTACGACGTGCTACACCTGGTTGAGTTCTACCTGGAGGAAGGGATCACTGATGAAGAAGCCATCTCC CTCATCGACCTGGAGGTGCTGAGACCCAAGCGGGATGACAGACAGCTAGAGATTGCAAACAACAGCTCCC AGATTCTGCGGCTAGTGGAGACCAAGGACTCCATCGGAGATGAGGACCCGTTCACAGCTAAGCTGAGCTT TGAGCAAGGTGGCTCAGAGTTCGTGCCAGTGGTGGTGAGCCGGCTGGTGCTGCGCTCCATGAGCCGCCGG GATGTCCTCATCAAGCGATGGCCCCCACCCCTGAGGTGGCAATACTTCCGCTCACTGCTGCCTGACGCCT CCATTACCATGTGCCCCTCCTGCTTCCAGATGTTCCATTCTGAGGACTATGAGTTGCTGGTGCTTCAGCA TGGCTGCTGCCCCTACTGCCGCAGGTGCAAGGATGACCCTGGCCCATGA >OriGene 5' read for NM_018262 unedited
GGTCAGCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGAGCCGTA AGGGAAGCCGTGATGAGGGCCGTGTTGACGTGGAGAGATAAAGCCGAGCACTGTATAAAT GACATCGCATTTAAGCCTGATGGAACTCAACTGATTTTGGCTGCCGGAAGCAGATTACTG GTTTATGACACCTCTGATGGCACCTTACTTCAGCCCCTCAAGGGACACAAAGACACTGTG TACTGTGTGGCATATGCGAAGGATGGCAAGCGCTTTGCTTCTGGATCAGCTGACAAAAGC GTTATTATCTGGACATCAAAACTGGAAGGCATTCTGAAGTACACGCACAATGATGCTATA CAATGTGTCTCCTACAATCCTATTACTCATCAACTGGCATCTTGTTCCTCCAGTGACTTT GGGTTGTGGTCTCCTGAACAGAAGTCTGTCTCCAAACACAAATCAAGCAGCAAGATCATC TGCTGCAGCTGGACAAATGATGGTCAGTACCTGGCGCTGGGGATGTTCAATGGGATCATC AGCATACGGAACAAAAATGGCGAGGAGAAAGTAAAGATCGAGCGGCCGGGGGGCTCCCTC TCGCCAATATGGTCCATCTGCTGGAACCCTTCAAGAGAGGAACGTAATGACATCCTGGCT GTGGCTGACTGGGGACAGAAAGTTTCCTTCTACCAGCTGAGTGGAAAACAGATTGGAAAG GGATCGGCACTGAACTTTGACCCCTGCTGCATCAGCTACTTTACTAAAGGCGAGTACATT TTGCTGGGGGGTTCAGACCAGCAAGTATCTCTTTTCACCAAGNATGNAGTGCGGCTTGGG ACTGTTTGGGAGCAGAACTCCTGGTGGGGACGTGTCAAGCGAAACCGGATTCCACTATGT GGGTGGTCGGCT >OriGene 3' read for NM_018262 unedited
GCTAAAGCCTTTAGTCGCCTTACAGCCCAGCACACCCCAGGCGGGCAGAGGGTGCATGTC CGTCCCCAGGATGCTGGTCATGGGCCAGGGTCATCCTTGCACCTGCGGCAGTAGGGGCAG CAGCCATGCTGAAGCACCAGCAACTCATAGTCCTCAGAATGGAACATCTGGAAGCAGGAG GGGCACATGGTAATGGAGGCGTCAGGCATTTTTTTATCGGAAGTATTGCCACCTCAGGGG TGGGGGCCATCGCTTGATGAGGACATCCCGGCGGCTCATGGAGCGCAGTACCAGCCGGCT CACCACCACTGGCACCAACCTCTGACCCCCTTGTTAAAGCTAACTTACTGGAAACGGCCT TATCCCCAGGGAGTCCTGGGTTCCACTACCCAAAAATTGGAACCTGTGGCGGCAATCCCA AACGTTGGCCTCCCCTTGGGCCCCACCCCCCCCGCCCAAAAGGAAAAGGCTTTTTCACAT TGCACCCTTTCTTCCGGAAAAACCCCCCAAGCGTCCCCCCCCCCTGCAAAAGGGGGCAAA ACATACGGTGTTCGCCACTTATCCCACACCTTTCCCCCCTTTCATCCCCTGTTTTCTGGG TTTTACTTGCCCCCAGGGCCCCCCCCCCCCCTTTCTTTAAAGGTCCTCCCCCCCTTTTTT TCCTCCCCCCTCTCCCTTTCTTTTCCCCCATTCCGCCCTTTCCTCCCCCCCTCTCTTTTC CCCCACTCGCGCCCCTCCCCCCCCCCCCTTTCCCTCCACCATGTTTTTTTCCTTTCCCTC CCCCCCCCTTTGTCATCACTCCCCTCCTTCTCCTTTTTCGCCCCGCCCCCTCCTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_018262 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018262.2, NP_060732.2 |
| RefSeq Size | 3837 bp |
| RefSeq ORF | 3549 bp |
| Locus ID | 55764 |
| UniProt ID | Q9HBG6 |
| Domains | WD40 |
| Gene Summary | This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This cytoplasmic protein contains seven WD repeats and an AF-2 domain which function by recruiting coregulatory molecules and in transcriptional activation. Mutations in this gene cause cranioectodermal dysplasia-1. A related pseudogene is located on chromosome 3. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 5' coding region, compared to variant 1. It encodes isoform 3, which lacks two internal segments and is shorter than isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221778 | IFT122 (Myc-DDK-tagged)-Human intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 3 |
CNY 8232.00 |
|
| RC221778L3 | Lenti ORF clone of Human intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 3, Myc-DDK-tagged |
CNY 13680.00 |
|
| RC221778L4 | Lenti ORF clone of Human intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 3, mGFP tagged |
CNY 13680.00 |
|
| RG221778 | IFT122 (tGFP-tagged) - Human intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 3 |
CNY 9832.00 |
