IMPACT (NM_018439) Human Untagged Clone
CAT＃: SC113513
IMPACT (untagged)-Human Impact homolog (mouse) (IMPACT)
CNY 2400.00
CNY 2950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RWDD5 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113513 sequence for NM_018439 edited (data generated by NextGen Sequencing)
ATGGCTGAGGGGGACGCAGGGAGCGACCAGAGGCAGAATGAGGAAATTGAAGCAATGGCA GCCATTTATGGCGAGGAGTGGTGTGTCATTGATGACTGTGCCAAAATATTTTGTATTAGA ATTAGCGACGATATAGATGACCCCAAATGGACACTTTGCTTGCAGGTGATGCTGCCGAAT GAATACCCAGGTACAGCTCCACCTATCTACCAGTTGAATGCTCCTTGGCTTAAAGGGCAA GAACGTGCGGATTTATCAAATAGCCTTGAGGAAATATATATTCAGAATATCGGTGAAAGT ATTCTTTACCTGTGGGTGGAGAAAATAAGAGATGTTCTTATACAAAAATCTCAGATGACA GAACCAGGCCCAGATGTAAAGAAGAAAACTGAAGAGGAAGATGTTGAATGTGAAGATGAT CTCATTTTAGCATGTCAGCCGGAAAGTTCGGTTAAAGCATTGGATTTTGATATCAGTGAA ACTCGGACAGAAGTAGAAGTAGAAGAATTACCTCCGATTGATCATGGCATTCCTATTACA GACCGAAGAAGTACTTTTCAGGCACACTTGGCTCCAGTGGTTTGTCCCAAACAGGTGAAA ATGGTTCTTTCCAAATTGTATGAGAATAAGAAAATAGCTAGTGCCACCCACAACATCTAT GCCTACAGAATATATTGTGAGGATAAACAGACCTTCTTACAGGATTGTGAGGATGATGGG GAAACAGCAGCTGGTGGGCGTCTTCTTCATCTCATGGAGATTTTGAATGTGAAGAATGTC ATGGTGGTAGTATCACGCTGGTATGGAGGGATTCTGCTAGGACCAGATCGCTTTAAACAT ATCAACAACTGTGCCAGAAACATACTAGTGGAAAAGAACTACACAAATTCACCTGAGGAG TCATCTAAGGCTTTGGGAAAGAACAAAAAAGTAAGAAAAGACAAGAAGAGGAATGAACAT TAA Clone variation with respect to NM_018439.3 >OriGene 5' read for NM_018439 unedited
GGTTTACATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCCCCGCCCC CGCGCTCCGGACCTGGCAGGCGGCGGCTGCAGGGCAGGTCCAGGGGCCACATGGCTGAGG GGGACGCAGGGAGCGACCAGAGGCAGAATGAGGAAATTGAAGCAATGGCAGCCATTTATG GCGAGGAGTGGTGTGTCATTGATGACTGTGCCAAAATATTTTGTATTAGAATTAGCGACG ATATAGATGACCCCAAATGGACACTTTGCTTGCAGGTGATGCTGCCGAATGAATACCCAG GTACAGCTCCACCTATCTACCAGTTGAATGCTCCTTGGCTTAAAGGGCAAGAACGTGCGG ATTTATCAAATAGCCTTGAGGAAATATATATTCAGAATATCGGTGAAAGTATTCTTTACC TGTGGGTGGAGAAAATAAGAGATGTTCTTATACAAAAATCTCAGATGACAGAACCAGGCC CAGATGTAAAGAAGAAAACTGAAGAGGAAGATGTTGAATGTGAAGATGATCTCATTTTAG CATGTCAGCCGGAAAGTTCGGTTAAAGCATTGGATTTTGATATCAGTGAAACTCGGACAG AAGTAGAAGTAGAAGAATTACCTCCGATTGATCATGGCATTCCTATTACAGACCGAAGAA GTACTTTTCAGGCACACTTGGCTCCAGTGGTTTGTCCCAACAGGTGAAAATGGTTCTTTC CAATTGTATGAGATAAGAANATAGCTAGTGCCACCCCACACATCTATGCCTACAGAATAT ATTGTGAGGATAAACAGACCTTCTTACANGATTGTGAGGATGATGGGGAAACAGCAGCTG GTGGGCGTCCTCTTCATCTCATGGAGATTTTGATGTGAAAGATGTCATGTGGGGTGTATC ACCCTGTTATGAAC >OriGene 3' read for NM_018439 unedited
GCGGGCCGCAATTTAGNATCGAGTTTTTTTTTTTTTTTTTTAAGCCATAGAAGAATATTT ATTGACATGGAAAATGTTAACAATATACTTCTATATGAAATATGTAGGCTACAAAACAGT ATATACAGTTTAATACCATTTTTATGGAAAGAAAAATAACCATATATACAAAATCATGCA TAAGAAAAAAATAATATAAGGATGTACATACCAAATATTAATAATAATGGCTATCTCTGG ATAGTGGAATCAGAGGGATTATGTAATTTTCCTGATAAATTTTCCTGTCCTCCAAACAGC ATCCGCTTCATACTATTATTTCTTGGTTGTAATTAGTTTGATATAATTCTCTTCAGAAAG GCTCTGTTTCACTATATATACCTCAAAGCATACTTTTGATGCAGCTTCTGCAATTCCCAT CTAAAAAGTAGATAACACTTGCTCTTATATTCTGGCATATGAAGACTATTTGTAATTAAC ACACTATAAAATATGTCAAAGCAGGCCAGGCATGGTGGCTCACACCTGTAATTCCAAAAC CTTGGCAGGAAGATCGATTGAGGCCAGGAGCTCAAGACGAGCCTGNGCAACATAGAAAGA CCCTATCTTTACAAAAAAAACTTTAAAAATTAGCCCAGTGTAATAGCACATGCCTGTCTG NTATCCCAGCTACTTGGCAGGCTGGAAGGTCAAGGCTGCANTGAGCCATGATCATGCCAC TGCACTCCAACCTAGGTGACAGAGCAAGAACTCATCTCTAAAAAAAATTTTTAAATAAAG CAAATATGCCACAGCATAAATCTGAATGTAAAAATTATTATTTGGGAGACTGAAAAATCT CCTAATCAAGACAAAATTTAAATTGAGGAAAAAAATACTATCTTTATTAGTTCAGGTTCC ATAAAAAATAAATGTGAAAAACCTCCAATTTAAAACTGGAAATTTTCCTCCTTCCTTTTA CAAACAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_018439 |
| Insert Size | 3700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018439.1, NP_060909.1 |
| RefSeq Size | 3683 bp |
| RefSeq ORF | 963 bp |
| Locus ID | 55364 |
| UniProt ID | Q9P2X3 |
| Domains | UPF0029, RWD |
| Gene Summary | Translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment and glucose deprivation. Plays a role as a negative regulator of the EIF2AK4/GCN2 kinase activity; impairs GCN1-mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis. May be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis. Through its inhibitory action on EIF2AK4/GCN2, plays a role in differentiation of neuronal cells by stimulating neurite outgrowth.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204631 | IMPACT (Myc-DDK-tagged)-Human Impact homolog (mouse) (IMPACT) |
CNY 2400.00 |
|
| RC204631L3 | Lenti ORF clone of Human Impact homolog (mouse) (IMPACT), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC204631L4 | Lenti ORF clone of Human Impact homolog (mouse) (IMPACT), mGFP tagged |
CNY 5890.00 |
|
| RG204631 | IMPACT (tGFP-tagged) - Human Impact homolog (mouse) (IMPACT) |
CNY 4370.00 |
|
| SC324621 | IMPACT (untagged)-Human Impact homolog (mouse) (IMPACT) |
CNY 2400.00 |
