RBM16 (SCAF8) (NM_014892) Human Untagged Clone
CAT＃: SC114740
SCAF8 (untagged)-Human SR-related CTD-associated factor 8 (SCAF8)
CNY 8856.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RBM16 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114740 sequence for NM_014892 edited (data generated by NextGen Sequencing)
ATGGAGGCCGTGAAGACCTTCAATAGCGAGTTGTATTCCCTGAATGACTATAAACCACCC ATTTCGAAAGCGAAAATGACCCAAATTACTAAGGCAGCCATCAAAGCTATTAAGTTCTAT AAACATGTGGTACAGAGTGTTGAGAAGTTTATTCAGAAATGTAAACCAGAATACAAAGTA CCTGGACTTTATGTTATTGACTCCATTGTGCGACAATCCCGACATCAGTTTGGTCAAGAA AAGGATGTGTTTGCACCCAGATTTAGTAATAACATCATTAGCACTTTCCAGAATTTATAT CGTTGCCCTGGGGATGACAAGAGTAAAATAGTGAGAGTACTAAACTTATGGCAGAAGAAT AATGTATTTAAGAGTGAGATTATTCAGCCCCTTTTGGATATGGCAGCCGGGATTCCGCCT CCAGTTGTCACACCTGTTTTGGCCAGCACTACCACTGCTATGAGCAATACTCCAGGAACT CCTGTGACACCTGTTACTCCGGCCAATGTGGTCCAAGGCTTACCTGATCCGTGGGTATCT CAGATAACAAATACAGATACACTTGCGGCTGTAGCTCAGATCTTGCAAAGTCCTCAAGGC CAGCAGCTTCAACAATTAATACAAACCTTACAGATACAACAACAGAAGCCCCAGCCTTCC ATTCTGCAGGCCCTAGATGCTGGTCTTGTTGTTCAGTTGCAAGCTCTTACGGCACAACTT ACAGCTGCAGCTGCAGCTGCCAACACTCTTACTCCCTTAGAACAGGGAGTCTCCTTTAAC AAGAAGTTGATGGATAGGTTTGATTTTGGGGAAGACTCTGAGCATAGTGAAGAACCCAAA AAGGAAATTCCAGCTTCACAACTTTCTCACGTTTCAGAATCTGTGAACAATTCCATTTTT CATCAGATAGCAGAACAACTACAACAGCAAAACCTAGAACATCTCAGACAGCAGCTCTTG GAGCAGCAACAGCCTCAAAAGGCCACTCCTCAGGATAGTCAGGAAGGAACCTTTGGGTCA GAGCATTCAGCGTCACCATCACAAGGGAGTAGTCAGCAGCATTTTCTTGAACCTGAAGTC AATTTGGATGATTCCATAGATATTCAGCAACAGGATATGGATATAGATGAAGGGCAAGAT GGAGTGGAAGAGGAGGTCTTTGAACAAGAAGCTAAGAAAGTGGCGGTTCGCTCAAGATCA AGAACACATTCACGATCTCGTTCAAGATCACCAAGAAAACGAAGGTCTAGGTCACGGTCT GGCTCTAGAAAGCGTAAACACAGAAAGCGATCACGCTCCCGCTCAAGAGAAAGAAAGAGG AAATCATCACGGTCGTATTCAAGTGAAAGGAGAGCCAGAGAAAGGGAGAAAGAACGACAG AAAAAGGGATTACCTCCAATTAGATCTAAAACACTAAGTGTATGTAGTACTACTCTCTGG GTTGGGCAAGTGGACAAGAAGGCAACACAGCAAGACTTAACCAACCTGTTTGAAGAGTTT GGACAGATTGAATCCATTAATATGATTCCTCCCCGGGGCTGTGCTTATGTCTGCATGGTT CATCGACAAGATGCATTTCGAGCTCTTCAGAAACTCAGTTCTGGATCATATAAAATTGGG TCCAAGGTCATTAAGATCGCTTGGGCTTTAAACAAAGGTGTAAAAACAGAATACAAACAA TTCTGGGATGTGGATCTTGGAGTTACATATATACCATGGGAAAAAGTTAAAGTGGATGAC TTGGAAGGTTTTGCAGAAGGAGGCATGATTGATCAGGAGACTGTAAATACTGAGTGGGAA ACTGTGAAAAGCTCAGAACCTGTTAAAGAGACGGTCCAGACAACTCAGAGCCCAACTCCA GTTGAAAAGGAGACAGTGGTCACAACCCAGGCAGAGGTTTTCCCTCCTCCTGTTGCTATG TTGCAGATTCCAGTGGCGCCAGCCGTGCCTACAGTTAGTTTAGTCCCACCAGCATTTCCT GTGTCGATGCCGGTTCCTCCTCCTGGATTCAGTCCAATCCCTCCACCTCCTTTTTTAAGA GCAAGTTTTAACCCTTCACAACCACCACCTGGTTTCATGCCGCCTCCAGTTCCCCCACCT GTTGTGCCACCCCCTACGATTCCACCAGTAGTACCAACATCTTTAGTGCAGCCGTCATTA TCCATGACACCGGAAACTGTGAAAGATGTTGGATTTGGTAGCCTTGTTATACCAGGCGGT TCTGTTGCCAGCAATCTTGCTACTTCCGCTCTGCCAGCTGGAAATGTTTTTAATGCTCCA ACTAAACAGGCAGAGCCTGAAGAAAAAGTACCTCATCTTATAGACCACCAGATTTCTTCT GGTGAAAACACCAGATCAGTGATTCCAAATGATATTTCAAGTAATGCTGCAATTTTAGGA GGACAGCCGCCAAATGTGACAAGCAATTCTGGAATTCTGGGAGTCCAAAGACCAAATGTA TCAAGTAATTCTGAAATTCTTGGGGTCCGGCCATCTAATGTTTCCAGTAGTTCTGGGATT ATTGCAGCCCAACCACCAAATATTCTAAATAACTCTGGAATATTGGGAATACAGCCACCC AGTGTGTCAAATAGTTCTGGACTTTTGGGAGTGCTACCCCCAAATATACCTAACAATTCT GGACTTGTAGGAGTACAGCCACCAAATGTTCCAAATACTCCTGGACTTCTGGGAACACAG CCACCAGCTGGACCTCAAAACTTACCCCCTTTAAGTATCCCTAATCAAAGGATGCCCACA ATGCCAATGTTAGACATTCGTCCGGGACTAATACCACAGGCACCTGGGCCAAGATTCCCT TTAATACAGCCTGGAATTCCACCCCAACGGGGAATCCCACCCCCATCGGTACTTGATTCA GCTCTTCATCCACCACCCCGTGGACCTTTTCCTCCAGGAGATATTTTTAGTCAACCAGAA AGACCTTTTTTAGCTCCTGGAAGACAAAGCGTAGACAATGTTACTAACCCAGAAAAAAGG ATACCACTTGGGAATGATAACATTCAACAGGAAGGAGATAGAGATTACCGGTTTCCTCCT ATAGAAACCAGGGAAAGCATTAGTAGACCTCCCCCTGTGGATGTTAGAGATGTGGTTGGG CGGCCTATAGATCCAAGAGAAGGTCCTGGACGGCCTCCACTAGATGGTAGGGATCATTTT GGAAGACCTCCTGTAGATATAAGAGAGAATCTTGTGAGGCCAGGTATAGATCATCTTGGT CGAAGAGACCACTTTGGCTTTAATCCAGAGAAGCCCTGGGGGCATAGAGGAGATTTTGAT GAGAGAGAGCATCGGGTTCTACCGGTCTATGGTGGTCCAAAAGGCTTACATGAAGAAAGA GGTAGATTTCGGTCTGGAAACTATCGATTTGATCCTAGAAGTGGTCCTTGGAACCGAGGA TTTGGACAAGAAGTTCACAGAGATTTTGATGACCGCAGAAGACCCTGGGAGAGGCAAAGG GATAGGGATGACAGAGATTTTGATTTCTGCAGAGAAATGAATGGAAATCGTCTTGGACGA GACAGAATTCAAAACACTTGGGTTCCCCCTCCTCATGCTCGGGTTTTTGATTATTTTGAA GGGGCCACTTCTCAACGAAAAGGTGATAATGTGCCTCAGGTTAATGGTGAAAATACAGAG AGACATGCTCAGCCACCACCTATACCAGTACAGAATGATCCTGAACTTTATGAAAAACTG ACATCTTCAAATGAAATAAACAAGGAGAAGAGTGACACAGTTGCTGATATAGAAAGTGAA CCAGTGGTAGAAAGCACAGAAACTGAGGGGACATAA Clone variation with respect to NM_014892.3 >OriGene 5' read for NM_014892 unedited
GGGGTGCAACATATGTATACGACTCACTATAGGCGGCCGCGAATCGGCACGAGGCCCGCC CCGGCAGCGCCTCTGTTCCCTAGAACGGCGCTCCCCCCGCCCTAGCGGCCATGCCGGTGC CGCTCTGCCGCTGAGGGAGCCCTTCCCCGCCAGCGCGTGCCCTTCCACTCCGCCCCGAGG TCGCAGCGGCCCGCTCTCCCGCCAGCGCCCCCTCCTCGCGGCCACGCAGCAGCCCGCGTC TCGCTCTCCCCACCCAGTGCAGTGGCCGCCGCCTCTTCCGCCGCCGGGCTCGGGGCCTCC GCAGCGACAACATGGAGGCCGTGAAGACCTTCAATAGCGAGTTGTATTCCCTGAATGACT ATAAACCACCCATTTCGAAAGCGAAAATGACCCAAATTACTAAGGCAGCCATCAAAGCTA TTAAGTTCTATAAACATGTGGTACAGAGTGTTGAGAAGTTTATTCAGAAATGTAAACCAG AATACAAAGTACCTGGACTTTATGTTATTGACTCCATTGTGCGACAATCCCGACATCAGT TTGGTCAAGAAAAGGATGTGTTTGCACCCAGATTTAGTAATAACATCATTAGCACTTTCC AGAATTTATATCGTTGCCCTGGGGATGACAAGAGTAAAATAGTGAGAGTACTAAACTTAT GGCAGAAGAATAATGTATTTAAGAGTGAGATTATTCAGCCCCTTTTTGATATGGCAGCCG GGATTCCGCCTCCAGTTGTCACACCTGTTTTGGCCAGCACTACCACTGCTATGAGCAAAT ACTCCAGAACTCCTGTGACACCCTGTACTCCGGCCATGTGGTCCAAGCCTACCTGATCCG TGGGATCTCANATACAATACAGAACACCTTGCGTTGTACTCAAACTTGCAAGATCTCAGG ACACAGCTCAACATTATA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014892 |
| Insert Size | 7000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014892.3, NP_055707.3 |
| RefSeq Size | 5018 bp |
| RefSeq ORF | 3816 bp |
| Locus ID | 22828 |
| UniProt ID | Q9UPN6 |
| Domains | RRM, RPR |
| Gene Summary | Anti-terminator protein required to prevent early mRNA termination during transcription (PubMed:31104839). Together with SCAF4, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins (PubMed:31104839). Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation (PubMed:31104839). Independently of SCAF4, also acts as a positive regulator of transcript elongation (PubMed:31104839).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) contains an alternate 5' exon and it thus differs in its 5' UTR and initiates translation at an downstream in-frame start codon, compared to variant 1. The encoded isoform (d) is shorter at the N-terminus, compared to isoform a. Both variants 4 and 5 encode isoform d. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209812 | SCAF8 (Myc-DDK-tagged)-Human SR-related CTD-associated factor 8 (SCAF8) |
CNY 8848.00 |
|
| RC209812L3 | Lenti-ORF clone of SCAF8 (Myc-DDK-tagged)-Human SR-related CTD-associated factor 8 (SCAF8) |
CNY 14540.00 |
|
| RC209812L4 | Lenti-ORF clone of SCAF8 (mGFP-tagged)-Human SR-related CTD-associated factor 8 (SCAF8) |
CNY 14540.00 |
|
| RG209812 | SCAF8 (tGFP-tagged) - Human SR-related CTD-associated factor 8 (SCAF8) |
CNY 13870.00 |
