ARHGAP32 (NM_014715) Human Untagged Clone
CAT＃: SC114870
ARHGAP32 (untagged)-Human Rho GTPase activating protein 32 (ARHGAP32), transcript variant 2
CNY 11688.00
货期*
现货
规格
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GC-GAP; GRIT; p200RhoGAP; p250GAP; PX-RICS; RICS |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_014715 edited
ATGAAGTCTCGTCCAACAAAACAGAAGCTGAAGCAGCGGGGAATCTTGAAAGAGAGGGTG TTTGGTTGTGACCTGGGGGAACACCTTCTAAATTCTGGTTTTGAAGTGCCGCAGGTTCTT CAAAGCTGCACAGCATTCATTGAGAGATATGGCATCGTGGATGGAATCTATCGCCTTTCT GGTGTTGCCTCCAATATCCAGAGACTACGCCATGAATTTGACTCTGAGCACGTCCCCGAC CTGACGAAAGAACCGTATGTTCAGGACATCCATTCTGTGGGTTCCCTATGTAAGCTGTAC TTCCGGGAACTCCCAAACCCTCTGCTTACCTACCAGCTGTATGAGAAATTTTCTGATGCA GTTTCAGCAGCAACAGATGAAGAAAGGCTGATAAAAATCCACGATGTCATCCAGCAGCTC CCCCCACCACACTACAGAACACTGGAGTTCCTGATGAGACACTTGTCTCTTCTAGCTGAC TATTGTTCCATCACAAATATGCATGCAAAAAATCTAGCAATTGTTTGGGCTCCAAACCTG TTAAGATCAAAACAGATAGAATCTGCCTGCTTCAGTGGAACAGCAGCTTTCATGGAAGTG AGGATTCAGTCTGTGGTTGTTGAGTTCATCCTGAATCACGTTGATGTGCTGTTCAGCGGC AGAATCAGCATGGCCATGCAAGAGGGGGCAGCTTCTCTATCAAGGCCCAAGTCCCTCCTG GTATCCTCTCCATCCACCAAACTGCTGACATTGGAAGAGGCCCAGGCACGAACACAAGCT CAGGTCAATTCTCCAATTGTGACGGAAAATAAATATATCGAAGTAGGAGAAGGACCTGCT GCACTTCAGGGGAAATTTCATACCATAATTGAGTTCCCACTTGAAAGAAAGAGGCCTCAA AATAAGATGAAAAAGTCTCCTGTGGGTAGCTGGCGTTCCTTTTTCAACTTGGGGAAATCA TCATCTGTTTCTAAACGAAAGCTGCAGCGGAATGAGAGTGAGCCTTCAGAGATGAAAGCC ATGGCTCTGAAAGGTGGCAGGGCAGAAGGAACCCTCCGTTCAGCTAAAAGTGAGGAGTCT CTTACATCTCTCCATGCAGTTGATGGTGATTCTAAGCTCTTCCGACCCAGAAGACCCAGA TCTTCCAGTGATGCACTGTCTGCCTCTTTTAATGGAGAAATGCTGGGGAACCGCTGTAAC TCCTATGATAATCTGCCTCATGACAATGAGAGTGAGGAGGAAGGAGGGCTGCTTCATATC CCAGCCCTTATGTCTCCTCATTCAGCTGAGGATGTTGACTTGAGCCCACCAGACATTGGA GTAGCCAGCCTGGATTTTGATCCAATGTCATTTCAATGTAGTCCTCCTAAGGCCGAATCA GAATGTCTGGAGAGTGGTGCTTCCTTTTTAGATTCACCAGGATACTCCAAGGATAAACCA AGTGCCAATAAAAAGGATGCAGAAACAGGTAGTAGCCAATGTCAGACTCCAGGAAGCACA GCAAGCTCTGAACCTGTCTCTCCTCTTCAGGAGAAACTGAGTCCATTCTTTACCCTGGAC TTGAGCCCAACTGAAGATAAATCATCTAAGCCATCCTCCTTTACTGAAAAGGTCGTCTAT GCTTTCTCTCCGAAGATAGGACGGAAATTAAGCAAATCACCTTCTATGAGCATATCTGAG CCAATTTCAGTGACCCTACCACCACGGGTGTCAGAAGTCATTGGTACAGTCTCAAATACC ACAGCTCAGAATGCATCATCTTCAACCTGGGACAAATGCGTTGAAGAAAGGGATGCCACA AATAGATCCCCCACCCAGATAGTAAAGATGAAAACAAATGAGACAGTTGCCCAAGAAGCA TATGAATCTGAAGTCCAGCCCCTGGACCAGGTGGCTGCTGAAGAAGTAGAATTGCCAGGG AAAGAGGATCAGTCTGTCTCAAGCAGTCAGAGTAAGGCTGTAGCTTCTGGACAGACTCAG ACAGGAGCAGTTACCCATGACCCCCCTCAGGATTCCGTTCCTGTCAGTTCAGTCTCTCTT ATCCCACCACCACCGCCTCCGAAAAATGTTGCCCGAATGTTGGCGCTAGCATTAGCTGAG TCCGCACAGCAAGCCTCAACTCAGTCATTGAAGAGACCAGGGACCTCTCAGGCTGGGTAT ACAAATTATGGAGACATAGCGGTGGCTACAACTGAAGATAATCTGTCCAGTTCTTACTCT GCAGTTGCTCTAGATAAGGCCTATTTCCAAACCGATCGACCAGCAGAGCAGTTCCACCTC CAGAATAATGCACCAGGAAACTGTGACCATCCTCTACCAGAGACAACAGCTACTGGGGAT CCTACCCATTCCAACACAACTGAATCTGGGGAGCAACATCACCAAGTAGACTTAACAGGG AATCAGCCACATCAAGCATATTTATCTGGGGACCCAGAGAAGGCCAGAATTACTTCAGTT CCCTTAGACTCAGAGAAGTCTGATGATCATGTAAGTTTCCCTGAAGACCAGTCTGGGAAG AACAGTATGCCAACTGTCTCCTTCTTGGATCAGGACCAGTCTCCACCCCGTTTCTACAGT GGAGATCAGCCTCCTTCTTATCTTGGTGCAAGTGTGGATAAACTCCATCACCCTTTAGAA TTTGCAGACAAATCTCCCACACCTCCTAATTTACCTAGCGATAAAATCTACCCTCCTTCT GGGTCCCCCGAAGAGAATACCAGCACAGCCACCATGACTTACATGACAACTACTCCAGCA ACAGCCCAAATGAGCACCAAGGAAGCCAGCTGGGATGTGGCTGAACAACCCACCACTGCT GATTTTGCTGCTGCCACACTTCAGCGCACGCACAGAACTAATCGTCCCCTTCCCCCTCCG CCTTCCCAGAGATCTGCAGAGCAGCCACCAGTTGTGGGGCAGGTACAAGCAGCAACCAAT ATAGGATTAAATAATTCCCACAAGGTTCAAGGAGTAGTTCCAGTTCCAGAGAGGCCACCT GAACCTCGAGCCATGGATGACCCTGCGTCTGCCTTCATCAGTGACAGTGGTGCTGCTGCT GCTCAGTGTCCCATGGCTACAGCTGTCCAGCCAGGCCTGCCTGAGAAAGTGCGGGACGGT GCCCGGGTCCCGCTGCTGCACCTGCGCGCCGAGTCTGTCCCTGCGCATCCCTGTGGCTTT CCTGCACCACTGCCCCCCACCAGGATGATGGAGAGTAAGATGATTGCTGCCATACACTCC AGCAGTGCAGATGCCACCAGCAGTTCAAATTATCATTCCTTTGTCACTGCTTCATCCACC TCTGTGGACGATGCATTGCCTTTACCACTTCCTGTCCCACAACCTAAGCATGCTTCTCAG AAAACAGTTTACTCCTCCTTTGCTAGGCCCGATGTCACCACTGAACCCTTTGGTCCAGAT AACTGTTTGCATTTCAATATGACTCCAAACTGCCAGTACCGTCCCCAGAGTGTACCTCCC CATCACAATAAATTGGAGCAGCACCAAGTGTATGGTGCCAGGTCAGAGCCACCAGCCTCC ATGGGTCTTCGTTATAACACATATGTGGCCCCAGGAAGAAACGCATCTGGACACCACTCC AAGCCATGCAGCCGGGTCGAGTATGTGTCTTCTTTGAGCTCCTCTGTCAGGAATACCTGT TACCCCGAAGACATTCCACCGTACCCTACCATCCGGAGAGTGCAGTCTCTCCATGCTCCG CCGTCTTCCATGATTCGCTCTGTTCCCATTTCACGGACAGAAGTTCCCCCAGATGATGAG CCAGCCTACTGCCCAAGACCTCTGTACCAATATAAGCCATATCAGTCCTCCCAGGCCCGC TCAGATTATCATGTCACTCAGCTTCAGCCTTACTTTGAGAATGGCCGGGTCCACTACAGG TATAGCCCATATTCCAGTTCTTCTAGTTCCTATTACAGTCCAGATGGGGCCCTGTGTGAT GTGGATGCCTATGGCACAGTCCAGTTGAGACCCCTTCACCGCCTTCCCAATCGAGACTTT GCTTTCTACAATCCTAGGCTGCAAGGAAAGAGCTTGTACAGTTATGCTGGTTTGGCTCCA CGTCCCCGGGCCAACGTGACTGGCTATTTCTCTCCCAACGACCATAATGTAGTCAGCATG CCTCCGGCTGCTGATGTGAAGCACACCTACACCTCATGGGATCTTGAGGACATGGAAAAA TACCGCATGCAGTCCATCCGGAGAGAGAGCCGTGCTCGGCAGAAGGTGAAAGGGCCTGTC ATGTCCCAATATGATAACATGACCCCGGCGGTGCAGGACGACTTGGGTGGGATCTATGTC ATCCATCTGCGTAGTAAATCAGATCCTGGGAAAACTGGACTTCTCTCAGTGGCAGAAGGA AAGGAGAGCCGCCATGCAGCCAAGGCCATCAGTCCCGAGGGAGAGGACCGCTTCTATAGG AGGCATCCCGAGGCAGAGATGGACAGAGCCCACCATCACGGAGGCCATGGTAGCACGCAG CCGGAGAAGCCATCCCTGCCTCAGAAGCAGAGCAGCCTGAGGAGCAGGAAGCTTCCTGAC ATGGGCTGCAGTCTTCCTGAGCACAGGGCACACCAAGAAGCAAGCCATAGGCAGTTCTGT GAGTCAAAGAATGGGCCCCCTTATCCCCAGGGAGCTGGCCAGTTAGATTATGGGTCCAAA GGGATTCCAGACACTTCTGAGCCAGTCAGCTACCACAACTCTGGAGTAAAATATGCTGCA TCCGGGCAAGAATCTTTAAGACTGAACCACAAAGAGGTAAGGCTCTCCAAAGAGATGGAG CGACCCTGGGTTAGGCAGCCTTCTGCCCCAGAGAAACACTCCAGAGACTGCTACAAGGAG GAAGAACACCTCACTCAGTCAATCGTCCCACCCCCTAAACCAGAGAGGAGTCATAGCCTC AAACTCCATCATACCCAGAACGTGGAGAGGGACCCCAGTGTGCTGTACCAGTACCAACCA CACGGCAAGCGCCAGAGCAGTGTGACTGTTGTGTCCCAGTATGATAACCTGGAAGATTAC CACTCCCTGCCTCAGCACCAGCGAGGAGTCTTTGGAGGGGGCGGCATGGGGACGTATGTG CCCCCTGGCTTTCCCCATCCACAGAGCAGGACCTATGCTACAGCGTTGGGTCAAGGGGCC TTCCTGCCCGCAGAGTTGTCCTTGCAGCATCCTGAAACACAGATCCATGCAGAATGA >OriGene 5' read for NM_014715 unedited
TCAAATTTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGGTTCTTAC GAACATTCATGAAGTCTCGTCCAACAAAACAGAAGCTGAAGCAGCGGGGAATCTTGAAAG AGAGGGTGTTTGGTTGTGACCTGGGGGAACACCTTCTAAATTCTGGTTTTGAAGTGCCGC AGGTTCTTCAAAGCTGCACAGCATTCATTGAGAGATATGGCATCGTGGATGGAATCTATC GCCTTTCTGGTGTTGCCTCCAATATCCAGAGACTACGCCATGAATTTGACTCTGAGCACG TCCCCGACCTGACGAAAGAACCGTATGTTCAGGACATCCATTCTGTGGGTTCCCTATGTA AGCTGTACTTCCGGGAACTCCCAAACCCTCTGCTTACCTACCAGCTGTATGAGAAATTTT CTGATGCAGTTTCAGCAGCAACAGATGAAGAAAGGCTGATAAAAATCCACGATGTCATCC AGCAGCTCCCCCCACCACACTACAGAACACTGGAGTTCCTGATGAGACACTTGTCTCTTC TAGCTGACTATTGTTCCATCACAAATATGCATGCAAAAAATCTAGNCATTGTTTGGGCTC CANACCTGTTAAGATCAAAACAGATAGAATCTGCCTGCTTCAGTGGAACAGCAGCTTTCA TGGAAGTGAGGATTCAGTCTGTGGTTGTTGAGTTCATCCTGAATCACGTTGATGTGCTNG TCAGCGGCAGAATCAGCATGGCCATGCCAGAGGNGGCAGCTTCTCTATCAGGNCCCAGTC CCTCCTGGTATCCTCTCCATCCACCAACTGCTGACATTGAAGAGGCCCAGCACGAACACA GCTCAGGTCATTCTTCCATTGTGACGGAAAATAATTATTCGAGTAAGAGAAGGACTGCTG CACTCAGGGGAAATTNCTACATNANTGAGNTCCCACTGNAGAAAGAGCCTCAAATA >OriGene 3' read for NM_014715 unedited
AGCTGTGGACCCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTAATGA AAGAAGTTGATAATTTAGGAAAACCAATGGTATGACATGTTTTACTAGAATTACAATTCA CCAAATCTTATTGAGGGGTGGGGTAAGAAGAAAACCTGAAGGCAGGCAATGCATTAAAAG CATCAATAGAGATTTCTGGTGCTAATAAAGTTCACTGACAATAAGAACTTTACTTTCTTC CACCTAAAGAAGTTTCCTTAAGTACTAACTTTTAAAAGTCCATTCTGTCATGATATGAGC CTGTTCACTGAACCGTGAGGAACAAGGATGAAAAATAAGAATAGAAAGAGTATGGTTCAG CCTGAGTCTAAGTGGTCTGGTGTTTTATGATGACTCTACCAAATGTTTAATTTAAAGTCT TAATTTCTTATTTTTAATTATAATGTTGCCAACTGTCTGACTGACCTTGAAGGATCAGGG ATTTTTCCACGACTCTAACTGAACACAAGATCCTTCTCAGACGGGGAGAATGAAGTGACA ACAGTGTGTCGATCTGCGCAAGTTGTGCAGCTACTGAAGGAGAAGCAGGAACACAACAGG GAATGCAGACGCCTACCAGGAAATCGATGTGAACTGCTCTCAATTATGCAGCAAATACTA AGAAGAAAGGACAATGCTGAATTCAAATTCAGTTAAAGGCAGTCCTCAGGCTCTTTCAAG CTAGCCCTTAGATGGCAGTGTGTCCTGAGACAGGTTTCTCTACTGGGTTTCAGCACGGGG CTTTACATCCTTCAGATCTTTTCTAAGACANAATGACAAGTCTATGATGGAANAGGGGTA TGAGCACGGAGGGGAAAAAGAGCTGAATTGTTACTTTATTACATTTTTAAAGTGGCAGGG TTTTATGNATTTTTTTTACCTTTTGGTATGAAAAAAAAACAGCCCTGCCACG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014715 |
| Insert Size | 7070 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014715.2, NP_055530.2 |
| RefSeq Size | 6480 bp |
| RefSeq ORF | 5217 bp |
| Locus ID | 9743 |
| UniProt ID | A7KAX9 |
| Protein Families | Druggable Genome |
| Gene Summary | RICS is a neuron-associated GTPase-activating protein that may regulate dendritic spine morphology and strength by modulating Rho GTPase (see RHOA; MIM 165390) activity (Okabe et al., 2003 [PubMed 12531901]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (2) lacks multiple exons in the 5' UTR and 5' coding region and has a unique exon for its 5' UTR, compared to variant 1. These differences cause translation initiation from a downstream in-frame start codon. The encoded protein (isoform 2) has a shorter N-terminus which lacks phox homology and src homology 3 domains, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The extent of this RefSeq transcript is supported by transcript alignments. |
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