CENPA (BC002703) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CENP-A; centromere protein A; centromere protein A, 17kDa; OTTHUMP00000122589
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC002703 edited GGCAGCCCGAGCAGGAGCCGTGGGACCGGGCGCCGGCACCCTCTGCGGCGTGTCATGGGC CCGCGCCGCCGGAGCCGAAAGCCCGAGGCCCCGAGGAGGCGCAGCCCGAGCCCGACCCCG ACCCCCGGCCCCTCCCGGCGGGGCCCCTCCTTAGGCGCTTCCTCCCATCAACACAGTCGG CGGAGACAAGGTTGGCTAAAGGAGATCCGAAAGCTTCAGAAGAGCACACACCTCTTGATA AGGAAGCTGCCCTTCAGCCGCCTGGCAAGAGAAATATGTGTTAAATTCACTCGTGGTGTG GACTTCAATTGGCAAGCCCAGGCCCTATTGGCCCTACAAGAGGCAGCAGAAGCATTTCTA GTTCATCTCTTTGAGGACGCCTATCTCCTCACCTTACATGCAGGCCGAGTTACTCTCTTC CCAAAGGATGTGCAACTGGCCCGGAGGATCCGGGGCCTTGAGGAGGGACTCGGCTGAGCT CCTGCACCCAGTGTTTCTGTCAGTCTTTCCTGCTCAGCCAGGGGGGATGATACCGGGGAC TCTCCAGAGCCATGACTAGATCCAATGGATTCTGCGATGCTGTCTGGACTTTGCTGTCTC TGAACAGCTGGAGTGCAGCAGCACGATCTCGGCTCACTGCAACCTCCAACTCCCAAGTTC AAGCGATTCTCCTGCCTCAGCCTCTCGGACCCTGTCTCTATTTTTTTTTAAATAAAGAAA TAAAAATTCTGTGTAACTTCATAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC002703 unedited CAAAATTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGCAGCCCGAGC AGGAGCCGTGGGACCGGGCGCCGGCACCCTCTGCGGCGTGTCATGGGCCCGCGCCGCCGG AGCCGAAAGCCCGAGGCCCCGAGGAGGCGCAGCCCGAGCCCGACCCCGACCCCCGGCCCC TCCCGGCGGGGCCCCTCCTTAGGCGCTTCCTCCCATCAACACAGTCGGCGGAGACAAGGT TGGCTAAAGGAGATCCGAAAGCTTCAGAAGAGCACACACCTCTTGATAAGGAAGCTGCCC TTCAGCCGCCTGGCAAGAGAAATATGTGTTAAATTCACTCGTGGTGTGGACTTCAATTGG CAAGCCCAGGCCCTATTGGCCCTACAAGAGGCAGCAGAAGCATTTCTAGTTCATCTCTTT GAGGACGCCTATCTCCTCACCTTACATGCAGGCCGAGTTACTCTCTTCCCAAAGGATGTG CAACTGGCCCGGAGGATCCGGGGCCTTGAGGAGGGACTCGGCTGAGCTCCTGCACCCAGT GTTTCTGTCAGTCTTTCCTGCTCAGCCAGGGGGGATGATACCGGGGACTCTCCAGAGCCA TGACTAGATCCAATGGATTCTGCGATGCTGTCTGGACTTTGCTGTCTCTGAACAGCTGGA GTGCAGCAGCACGATCTCGGCTCACTGCAACCTCCAACTCCCAAGTTCAAGCGATTCTCC TGCCTCAGCCTCTCGGACCCTGTCTCTATTTTTTTTTAAATAAAGAAATAAAAATTCTGT GTAACTTCATAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGNT TCCCTGACAGATCCCGGNTGGCATCCCTGTGACCCTNCCCAGTGCCTCTCCTGGCCTTGG NAAGTGCACTCAGTGCCCACCAGCCTGTCTATAAAAATAGTGCACATTTGCTGCTAGTGT CTCTTAATAGGAGGGGGG
Restriction Sites	Please inquire
ACCN	BC002703
Insert Size	768 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC002703.2, AAH02703.1
RefSeq Size	759 bp
Locus ID	1058
Gene Summary	Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015]

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