IFI27 (BC015492) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	2310061N23Rik; FAM14D; interferon, alpha-inducible protein 27; ISG12; ISG12A; P27
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC015492 edited GGCAGGAGCTACCCTTCCCGTGGCCCCGGACCTTGGGTGGGCTGTGGGCTCAGGGAGCGG AGGGGAGGCCTTAAGCATCCACTCTCTGCCCGGTGTTTTTGTTCTCATCAGGGAGCCTCA GATGGGAAGGGACTCGAGCCCCACCTGTCCCTGGACTCTGGAATGCCACGGAATTAACCC GAGCAGGCATGGAGGCCTCTGCTCTCACCTCATCAGCAGTGACCAGTGTGGCCAAAGTGG TCAGGGTGGCCTCTGGCTCTGCCGTAGTTTTGCCCCTGGCCAGGATTGCTACAGTTGTGA TTGGAGGAGTTGTGGCTGTGCCCATGGTGCTCAGTGCCATGGGCTTCACTGCGGCGGGAA TCGCCTCGTCCTCCATAGCAGCCAAGATGATGTCCGCGGCGGCCATTGCCAATGGGGGTG GAGTTGCCTCGGGCAGCCTTGTGGCTACTCTGCAGTCACTGGGAGCAACTGGACTCTCCG GATTGACCAAGTTCATCCTGGGCTCCATTGGGTCTGCCATTGCGGCTGTCATTGCGAGGT TCTACTAGCTCCCTGCCCCTCGCCCTGCAGAGAAGAGAACCATGCCAGGGGAGAAGGCAC CCAGCCATCCTGACCCAGCGAGGAGCCAACTATCCCAAATATACCTGGGGTGAAATATAC CAAATTCTGCATCTCCAGAGGAAAATAAGAAATAAAGATGAATTGTTGCAACTCAAAAAA AAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC015492 unedited NNGCGTCGGGATTTGTATACGACTTCATATAGGCGGCCGCGCATTCCCGGGGGATCGTCG ACCTTTCGTCCGGGCAGGAGCTACCCTTCCCGTGGCCCCGGACCTTGGGTGGGCTGTGGG CTCATGGAGCGGAGGGGAGGCCTTAAGCATCCACTCTCTGCCCGGTGTTTTTGTTCTCAT CAGGGAGCCTCAGATGGGAAGGGACTCGAGCCCCACCTGTCCCTGGACTCTGGAATGCCA CGGAATTAACCCGAGCAGGCATGGAGGCCTCTGCTCTCACCTCATCAGCAGTGACCAGTG TGGCCAAAGTGGTCAGGGTGGCCTCTGGCTCTGCCGTAGTTTTGCCCCTGGCCAGGATTG CTACAGTTGTGATTGGAGGAGTTGTGGCTGTGCCCATGGTGCTCAGTGCCATGGGCTTCA CTGCGGCGGGAATCGCCTCGTCCTCCATAGCAGCCAAGATGATGTCCGCGGCGGCCATTG CCAATGGGGGTGGAGTTGCCTCGGGCAGCCTTGTGGCTACTCTGCAGTCACTGGGAGCAA CTGGACTCTCCGGATTGACCAAGTTCATCCTGGGCTCCATTGGGTCTGCCATTGCGGCTG TCATTGCGAGGTTCTACTAGCTCCCTGCCCCTCGCCCTGCAGAGAATAGAACCATGCCAG GGGAGAAGGCACCCAGCCATCCTGACCCAGCGAGGAGCCAACTATCCCAAATATACCTGG GGTGAAATATACCAAATTCTGCATCTCCAGAGGAAAATAAGANATAAAGATGAATTGTTG CAACTCAAAAAAAAAAAAAAGGGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGG GTGGCATCCCTGTGACCCCTCCCCATGCCTCTCCTGGCCCTGGAAGTGCCACTTCATG
Restriction Sites	Please inquire
ACCN	BC015492
Insert Size	729 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC015492.1, AAH15492.1
RefSeq Size	729 bp
RefSeq ORF	357 bp
Locus ID	3429
Protein Families	Transmembrane
Gene Summary	Probable adapter protein involved in different biological processes (PubMed:22427340, PubMed:27194766). Part of the signaling pathways that lead to apoptosis (PubMed:18330707, PubMed:27673746, PubMed:24970806). Involved in type-I interferon-induced apoptosis characterized by a rapid and robust release of cytochrome C from the mitochondria and activation of BAX and caspases 2, 3, 6, 8 and 9 (PubMed:18330707, PubMed:27673746). Also functions in TNFSF10-induced apoptosis (PubMed:24970806). May also have a function in the nucleus, where it may be involved in the interferon-induced negative regulation of the transcriptional activity of NR4A1, NR4A2 and NR4A3 through the enhancement of XPO1-mediated nuclear export of these nuclear receptors (PubMed:22427340). May thereby play a role in the vascular response to injury (By similarity). In the innate immune response, has an antiviral activity towards hepatitis C virus/HCV (PubMed:27194766, PubMed:27777077). May prevent the replication of the virus by recruiting both the hepatitis C virus non-structural protein 5A/NS5A and the ubiquitination machinery via SKP2, promoting the ubiquitin-mediated proteasomal degradation of NS5A (PubMed:27194766, PubMed:27777077).[UniProtKB/Swiss-Prot Function]

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