Nebulette (NEBL) (NM_006393) Human Untagged Clone
CAT＃: SC124017
NEBL (untagged)-Human nebulette (NEBL), transcript variant 1
CNY 7072.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bA165O3.1; C10orf113; LASP2; LNEBL |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC124017 sequence for NM_006393 edited (data generated by NextGen Sequencing)
ATGAGGGTCCCTGTATTTGAGGATATAAAAGATGAAACTGAAGAAGAAAAGATAGGGGAA GAAGAAAATGAAGAAGACCAGGTCTTCTATAAGCCTGTTATTGAAGACTTAAGCATGGAA TTGGCCAGAAAATGCACGGAACTCATTAGCGATATCCGTTATAAAGAAGAGTTTAAAAAG TCCAAGGATAAGTGTACATTTGTGACTGACAGTCCTATGCTAAACCATGTAAAAAATATC GGTGCTTTTATTTCTGAGGCAAAATACAAAGGCACCATTAAAGCTGACCTTTCTAATTCT CTTTATAAGCGGATGCCAGCCACAATTGACAGTGTTTTTGCAGGAGAAGTTACACAGCTC CAGAGTGAGGTGGCCTACAAGCAGAAACATGATGCTGCCAAAGGATTCTCAGATTATGCC CACATGAAGGAGCCCCCTGAGGTTAAACATGCCATGGAGGTCAATAAACACCAGAGTAAT ATTTCTTATAGGAAAGACGTGCAGGACACCCACACGTACAGTGCAGAACTTGACCGACCA GACATCAAGATGGCAACCCAGATCTCTAAGATCATAAGCAATGCAGAATACAAGAAAGGA CAAGGAATAATGAATAAAGAGCCCGCTGTAATTGGAAGACCAGATTTTGAACATGCCGTG GAAGCTTCTAAACTTTCTAGTCAAATTAAATACAAAGAAAAATTTGATAATGAAATGAAG GATAAGAAACATCATTACAATCCTCTTGAAAGTGCTTCTTTTAGGCAGAATCAGCTTGCT GCTACACTGGCGAGCAATGTGAAGTACAAGAAAGACATTCAAAATATGCATGATCCAGTT TCAGATCTCCCAAATTTGTTGTTTTTAGACCATGTTTTGAAAGCCAGCAAAATGCTCAGC GGCCGAGAATATAAAAAGCTCTTTGAGGAAAACAAAGGAATGTATCATTTTGATGCAGAT GCTGTGGAACATCTGCACCATAAAGGCAATGCCGTCCTCCAAAGTCAGGTGAAATATAAA GAAGAATATGAGAAAAATAAGGGAAAGCCAATGCTTGAATTTGTTGAGACACCATCATAT CAAGCTTCAAAGGAGGCTCAAAAGATGCAAAGTGAAAAAGTTTACAAAGAGGATTTTGAG AAGGAGATTAAAGGAAGGTCATCACTGGATTTAGACAAGACTCCAGAATTTTTACATGTA AAGTACATCACCAACCTTCTGAGGGAGAAAGAATATAAAAAAGATTTGGAAAATGAGATA AAAGGGAAAGGAATGGAACTTAATTCAGAAGTTCTTGATATCCAAAGAGCAAAGCGAGCC TCTGAAATGGCAAGTGAGAAAGAATACAAGAAAGACCTGGAGTCAATAATTAAAGGGAAA GGAATGCAAGCTGGCACTGACACCCTTGAAATGCAGCATGCCAAGAAGGCTGCAGAGATA GCGAGTGAGAAAGACTATAAAAGAGATCTGGAGACTGAAATTAAAGGGAAAGGGATGCAG GTGAGCACAGACACTCTTGATGTCCAGAGAGCTAAGAAAGCATCCGAGATGGCCAGCCAG AAACAATACAAGAAGGACTTAGAAAATGAAATTAAAGGGAAAGGAATGCAAGTGAGCATG GATATCCCAGATATCCTTCGAGCCAAGAGGACATCTGAAATCTATAGCCAGAGAAAGTAT AAAGATGAAGCAGAGAAGATGCTTTCTAACTATTCTACCATAGCAGATACTCCTGAAATT CAGAGAATTAAGACAACTCAACAAAACATTAGTGCGGTATTTTATAAGAAAGAAGTGGGA GCTGGCACTGCAGTGAAAGATAGCCCAGAGATCGAACGAGTGAAGAAAAATCAGCAGAAT ATTAGTTCAGTGAAATACAAAGAAGAGATTAAACATGCAACAGCCATTTCTGATCCTCCA GAACTAAAGAGAGTTAAAGAAAACCAGAAGAACATCAGCAATCTCCAGTATAAAGAGCAA AACTACAAGGCCACTCCGGTAAGCATGACCCCGGAGATAGAGAGAGTGAGGCGAAACCAG GAGCAGCTGAGTGCGGTAAAATATAAGGGAGAACTTCAACGGGGAACTGCAATTTCTGAT CCACCAGAGCTGAAGAGGGCAAAAGAAAACCAGAAAAACATCAGCAATGTTTATTACAGA GGTCAGCTGGGAAGAGCTACCACTTTAAGTGTAACTCCTGAAATGGAAAGAGTGAAGAAG AATCAAGAAAATATTAGCTCGGTAAAATATACCCAGGACCATAAACAGATGAAAGGTAGA CCAAGTCTGATTTTAGATACACCTGCTATGAGACATGTTAAAGAAGCACAAAATCATATT TCAATGGTAAAATACCATGAAGATTTTGAAAAAACAAAGGGGAGAGGCTTTACTCCCGTC GTGGACGATCCTGTGACAGAGAGAGTGAGGAAGAACACCCAGGTGGTCAGCGATGCTGCC TATAAAGGGGTCCACCCTCACATCGTGGAGATGGACAGGAGACCTGGAATCATTGTTGAC CTCAAAGTTTGGCGCACAGATCCTGGCTCCATCTTCGACCTTGATCCCCTGGAAGACAAT ATTCAGTCTAGAAGTCTCCATATGCTCTCTGAAAAGGCGAGTCACTATAGGCGACACTGG TCTCGATCCCATTCCAGCAGTACTTTCGGTACAGGTCTCGGAGACGACAGGTCAGAAATC TCCGAGATTTACCCTAGCTTTTCATGCTGCAGTGAGGTAACAAGACCGTCTGATGAAGGA GCACCTGTTCTTCCCGGAGCCTATCAGCAAAGCCATTCCCAAGGCTATGGCTACATGCAC CAGACCAGTGTGTCATCCATGAGATCAATGCAGCATTCACCAAATCTAAGGACCTACCGA GCCATGTACGATTACAGTGCCCAGGATGAAGACGAGGTCTCCTTTAGAGACGGCGACTAC ATCGTCAACGTGCAGCCTATTGACGATGGCTGGATGTACGGCACAGTGCAGAGAACAGGG AGAACAGGAATGCTCCCAGCGAATTACATTGAGTTTGTTAATTAA Clone variation with respect to NM_006393.2 >OriGene 5' read for NM_006393 unedited
CAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGACGTCAG CATCTCAGGGCACTGTGGTATGGAAAAGGACGCCACATGAGTAAATTTTAAAAATATAAA TATTTTAAAGGGTAAAAATGAGGGTCCCTGTATTTGAGGATATAAAAGATGAAACTGAAG AAGAAAAGATAGGGGAAGAAGAAAATGAAGAAGACCAGGTCTTCTATAAGCCTGTTATTG AAGACTTAAGCATGGAATTGGCCAGAAAATGCACGGAACTCATTAGCGATATCCGTTATA AAGAAGAGTTTAAAAAGTCCAAGGATAAGTGTACATTTGTGACTGACAGTCCTATGCTAA ACCATGTAAAAAATATCGGTGCTTTTATTTCTGAGGCAAAATACAAAGGCACCATTAAAG CTGACCTTTCTAATTCTCTTTATAAGCGGATGCCAGCCACAATTGACAGTGTTTTTGCAG GAGAAGTTACACAGCTCCAGAGTGAGGTGGCCTACAAGCAGAAACATGATGCTGCCAAAG GATTCTCAGATTATGCCCACATGAAGGAGCCCCCTGAGGTTAAACATGCCATGGAGGTCA ATAAACACCAGAGTAATATTTCTTATAGGAAAGACGTGCAGGACACCCACACGTACAGTG CAGAACTTGACCGACCAGACATCAAGATGGGCACCCAGATCTCNTAGATCATAAGCCATG CGAGATACAAGAAAAGACCAGGAATAATGGAATAAGAGCCCCGCTGTAATTGGGAAGACC AGATTTTTGACATGCCGTGGAAGCTTCTAAACTTTCTAGTCAAAANAAATACCAAGAAAA ATTTGATAATGGAATGGAAGGTAAGAAACATCCTTTCCATCCCTCTGGAAGTGCCTCTTT TAAGCAGGATCAGCTTGCTGCTCCCCTGGGCAGACATGGGAAAGTTCAAGAAAGGACCTT CAA >OriGene 3' read for NM_006393 unedited
ACTTCCAGGCCAGGAGAGGCACTGGGGAGNGGTCACAGGGATGCCACCCGGGATCTGTTC AGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTGGTTTTTTTTTT ACGTCAGAGACTTTTATTTTAGCATCCTTGTCAGTTTACATAGCTTCTTACTTAACTAAA AGCTCTCCCACCTCCATATCACCTTAACCAAAACAACCACAATCTAGCCACTTTTAGATT ATCAGAAGCAAATCGCTAAGGTATAATTAATTTATGAAGTGAAGTTCATCTTGTGTAGTG AATCCAGCCTCACAATTATTCTATCTTACAATAGGCATTTTAAATCCCAAATCTAAAGTT AATGTTTATATAGCTCAACTCTATATAAATCCACAAAGAACCTTTCAGAAATAGATGGAA GAAGTCGGCATTTTCTAAAGTTCTAAATCTTTAGAGATAAAATACAATCAGCTAACACCT TTAATATCTGAGGAGCATCCCACTTCCCTACCACAAAAATATCAATCATGTGTAATAAAA TATTACTATAGAATAGGCAGCAACTTGCTTGCTTGGTTTTCTTTCCAAGAGCATACTAGA CAAACAAGAGTAATCAAAAAGGCACCTATATTTGTATATATCTGTATTACAGTTTTGGTA TATCTTTTATCCACTTTCTATAGGGATGTGTTATTAAAGATCACACACATTTTCTTCATC TTTTATTGAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_006393 |
| Insert Size | 7500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006393.1, NP_006384.1 |
| RefSeq Size | 8034 bp |
| RefSeq ORF | 3045 bp |
| Locus ID | 10529 |
| UniProt ID | O76041 |
| Gene Summary | This gene encodes a nebulin like protein that is abundantly expressed in cardiac muscle. The encoded protein binds actin and interacts with thin filaments and Z-line associated proteins in striated muscle. This protein may be involved in cardiac myofibril assembly. A shorter isoform of this protein termed LIM nebulette is expressed in non-muscle cells and may function as a component of focal adhesion complexes. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). This isoform (1) is also known as the sarcomeric isoform. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222431 | NEBL (Myc-DDK-tagged)-Human nebulette (NEBL), transcript variant 1 |
CNY 10070.00 |
|
| RC222431L3 | Lenti-ORF clone of NEBL (Myc-DDK-tagged)-Human nebulette (NEBL), transcript variant 1 |
CNY 11970.00 |
|
| RC222431L4 | Lenti-ORF clone of NEBL (mGFP-tagged)-Human nebulette (NEBL), transcript variant 1 |
CNY 11970.00 |
|
| RG222431 | NEBL (tGFP-tagged) - Human nebulette (NEBL), transcript variant 1 |
CNY 9368.00 |
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