MRPL20 (NM_017971) Human Untagged Clone
CAT＃: SC126679
MRPL20 (untagged)-Human mitochondrial ribosomal protein L20 (MRPL20), nuclear gene encoding mitochondrial protein
CNY 1200.00
CNY 2950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | L20mt; MRP-L20 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC126679 sequence for NM_017971 edited (data generated by NextGen Sequencing)
ATGGTCTTCCTCACCGCGCAGCTCTGGCTGCGGAATCGCGTCACCGACCGCTACTTTCGG ATCCAGGAGGTGCTGAAGCACGCCAGGCACTTCCGGGGAAGGAAAAATCGCTGCTACAGG TTGGCGGTCAGAACCGTGATTCGAGCCTTTGTGAAATGCACCAAAGCCCGATACCTGAAG AAAAAGAACATGAGGACCCTCTGGATTAATCGAATTACAGCTGCTAGCCAGGAACATGGA CTGAAGTATCCAGCGCTCATTGGGAATTTAGTTAAGTGCCAGGTGGAGCTCAACAGGAAA GTCCTAGCGGATCTGGCCATCTACGAGCCAAAGACTTTCAAATCTTTGGCTGCCTTGGCC AGTAGGAGGCGACACGAAGGATTTGCTGCTGCCTTGGGGGATGGGAAGGAACCTGAAGGC ATTTTTTCCAGAGTGGTGCAGTACCACTGA Clone variation with respect to NM_017971.3 >OriGene 5' read for NM_017971 unedited
GACCCGAATTCGGCACGAGAGCGGCGCTGCGCGTGCTTTGTTGAGTGTTCGGGACGCGGG CCTGCAGGCGCCATGGTCTTCCTCACCGCGCAGCTCTGGCTGCGGAATCGCGTCACCGAC CGCTACTTTCGGATCCAGGAGGTGCTGAAGCACGCCAGGCACTTCCGGGGAAGGAAAAAT CGCTGCTACAGGTTGGCGGTCAGAACCGTGATTCGAGCCTTTGTGAAATGCACCAAAGCC CGATACCTGAAGAAAAAGAACATGAGGACCCTCTGGATTAATCGAATTACAGCTGCTAGC CAGGAACATGGACTGAAGTATCCAGCGCTCATTGGGAATTTAGTTAAGTGCCAGGTGGAG CTCAACAGGAAAGTCCTAGCGGATCTGGCCATCTACGAGCCAAAGACTTTCAAATCTTTG GCTGCCTTGGCCAGTAGGAGGCGACACGAAGGATTTGCTGCTGCCTTGGGGGATGGGAAG GAACCTGAAGGCATTTTTTCCAGAGTGGTGCAGTACCACTGAGGACTGTTGCTGTATTGA TTAGGAAAAGAGACAGAGTAATTTGCAGTTTGTTTGATTTATACTTTTGTTTATCTACAA CCCAATAACAGACATGAGGGATGGCCCTGTCTCTCTGGGACAGAGCCTCAAAGATGATGT CCATGTTTTTGTGTGAATGAAACTCAAACACTCTTCAAAAAAAAAAA >OriGene 3' read for NM_017971 unedited
GCTATGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTGAAAAGGGTTTGAGTTTCATTCACACAAAACATGGACATCATCTTTGAGGC TCTGTCCCAAAAAGACAGGGCCATCCCTCATGTCTGTTATTGGGTTGTAGATAAACAAAA GTATAAATCAAACAAACTGCAAATTACTCTGTCTCTTTTCCTAATCAATACAGCAACAGT CCTCAGTGGTACTGCACCACTCTGGAAAAAATGCCTTCAGGTTCCTTCCCATCCCCCAAG GCAGCAGCAAATCCTTCGTGTCGCCTCCTACTGGCCAAGGCAGCCAAAGATTTGAAAGTC TTTGGCTCGTAAATGGCCAAATCCGCTAGGACTTTCCTGTTGAGCTCCACCTGGCACTTA ACTAAATTCCCAATGAGCGCTGGATACTTCAGTCCATGTTCCTGGCTAGCAGCTGTAATT CGATTAATCCAGAGGGTCCTCATGTTCTTTTTCTTCAGGTATCGGGCTTTGGTGCATTTC ACAAAGGCTCGAATCACGGTTCTGACCGCCAACCTGTAGCAGCGATTTTTCCTTCCCCGG AAGTGCCTGGCGTGCTTCAGCACCTCCTGGATCCGAAAGTAGCGGTCGGTGACGCGATTC CGCAGCCAGAGCTGCGCGGTGAGGAAGACCATGGCGCCTGCAGGCCCCGCGTCCGACACT TAACAACGCACGCGCAGCGCCGTTCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCG TATTACAAATTCTGACGGTTCACTAAACGAGCTCTGCTATATAGACCTNCCACCGTAACG CCTACCGGCCATTTGCGTCACGGGGCCGGNGGTATTACGACATTNTTGGAAGTCCCNCGT GATTTTGGTGCCAAANACACTTCCCATGACGTCATGGGGTGGAGACTGGGAATCCCCGGA GTCAACGNTTCCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_017971 |
| Insert Size | 800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017971.2, NP_060441.2 |
| RefSeq Size | 705 bp |
| RefSeq ORF | 450 bp |
| Locus ID | 55052 |
| UniProt ID | Q9BYC9 |
| Domains | Ribosomal_L20 |
| Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 21q. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016] Transcript Variant: This variant (1) encodes the longer isoform (1). |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204772 | MRPL20 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L20 (MRPL20), nuclear gene encoding mitochondrial protein |
CNY 1200.00 |
|
| RC204772L3 | Lenti ORF clone of Human mitochondrial ribosomal protein L20 (MRPL20), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC204772L4 | Lenti ORF clone of Human mitochondrial ribosomal protein L20 (MRPL20), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5890.00 |
|
| RG204772 | MRPL20 (tGFP-tagged) - Human mitochondrial ribosomal protein L20 (MRPL20), nuclear gene encoding mitochondrial protein |
CNY 2800.00 |
