VPS8 (NM_001009921) Human Untagged Clone
CAT＃: SC301440
VPS8 (untagged)-Human vacuolar protein sorting 8 homolog (S. cerevisiae) (VPS8), transcript variant 1
CNY 9776.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | KIAA0804 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_001009921 edited
GCCGGCGGTCCACGGCCGGAATGGCAGCAAGCTCAGAGATAATCATTCAGGTCTTCGTGA GCTAAGGCCAAACAAACAAAAAACACTTGCTTTGATGGACTGAATACTGTTATTAGAAGT AAATATGGAAAATGAACCAGACCATGAAAATGTGGAACAGAGCCTCTGTGCCAAGACGAG CGAAGAAGAGCTGAATAAGTCTTTCAATCTAGAAGCTTCACTTTCAAAATTCTCTTACAT AGATATGGACAAGGAACTGGAGTTCAAAAATGATCTGATTGATGACAAGGAGTTTGATAT TCCTCAAGTTGATACTCCTCCAACACTGGAAAGCATACTAAATGAGACTGATGATGAAGA TGAGTCTTTTATTCTTGAGGATCCTACATTGTTAAACATTGATACTATTGATTCTCACTC CTATGATACTTCATCTGTGGCAAGCTCAGATAGTGGTGACAGGACCAACTTAAAAAGGAA GAAGAAATTACCTGATTCTTTTTCACTTCATGGATCAGTTATGCGCCATTCACTTTTGAA GGGAAGTTCTGCCCAGATAGTGTCTGCAGCTGACAAAGTAGATGCTGGCTTGCCTACAGC AATTGCAGTATCCAGTCTGATAGCAGTGGGTACATCTCATGGATTGGCTTTAATATTTGA TCAGAATCAAGCTTTGCGACTCTGTCTGGGTAGCACTAGTGTTGGAGGTCAGTATGGCGC TATCTCTGCCCTCAGTATCAACAATGATTGCTCAAGACTTCTTTGTGGCTTTGCTAAAGG ACAGATCACCATGTGGGATTTGGCCAGTGGAAAACTTCTAAGATCAATAACAGATGCTCA TCCTCCAGGAACAGCAATATTGCATATCAAGTTTACAGATGATCCAACTCTTGCAATTTG CAACGACAGCGGAGGCTCTGTTTTTGAATTGACATTTAAGAGAGTGATGGGAGTGAGAAC CTGTGAATCTAGATGTCTGTTCAGTGGTTCCAAGGGTGAAGTCTGCTGTATTGAGCCTCT GCATTCTAAGCCTGAGTTGAAAGATCATCCCATCACACAGTTTTCATTATTGGCCATGGC ATCCTTGACAAAAATACTGGTCATTGGATTGAAACCATCCTTGAAAGTATGGATGACTTT TCCCTATGGCCGGATGGATCCTTCCAGTGTGCCACTGCTGGCCTGGCACTTTGTAGCAGT ACAAAATTACGTGAATCCCATGCTTGCCTTCTGCAGAGGAGATGTTGTTCATTTTCTATT GGTAAAGAGAGATGAATCTGGAGCAATACATGTTACTAAGCAAAAGCATCTTCACCTATA CTATGACCTCATCAACTTTACCTGGATAAATTCACGCACAGTTGTGCTCTTAGACAGCGT AGAGAAGTTGCATGTGATTGATCGGCAAACACAAGAGGAATTGGAGACAGTGGAGATCTC AGAAGTTCAGCTGGTCTACAATAGCAGCCATTTCAAATCACTAGCCACTGGAGGAAATGT TAGCCAGGCACTGGCTTTGGTTGGAGAGAAGGCTTGTTATCAATCCATCAGTAGCTATGG TGGTCAGATCTTTTATTTGGGGACAAAATCTGTTTATGTGATGATGCTGAGGAGCTGGAG AGAGAGAGTGGATCATCTCCTGAAACAAGATTGTCTTACAGAAGCGTTGGCTCTTGCGTG GTCTTTCCATGAAGGAAAAGCAAAAGCAGTAGTGGGATTATCAGGGGATGCCAGTAAGCG AAAGGCTATTGTTGCAGACCGGATGGTAGAAATCCTATTCCATTATGCAGATCGAGCTCT GAAAAAGTGCCCAGACCAAGGAAAAATCCAAGTGATGGAGCAGCATTTTCAGGATATGGT GCCTGTCATAGTTGATTACTGCCTTCTGCTGCAGCGAAAGGATCTTTTATTTAGTCAGAT GTATGATAAATTAAGTGAGAATTCAGTGGCCAAAGGAGTATTTTTGGAGTGCCTTGAGCC ATATATTTTAAGTGATAAATTGGTGGGAATCACACCCCAAGTAATGAAAGACTTGATTGT TCATTTCCAAGATAAAAAATTAATGGAAAATGTGGAAGCGCTCATTGTACATATGGATAT CACCAGCCTAGATATTCAGCAGGTAGTTCTCATGTGTTGGGAAAATCGTTTATATGATGC TATGATCTATGTCTACAACAGAGGCATGAATGAATTTATTAGTCCAATGGAGAAACTTTT CAGAGTCATTGCTCCTCCTCTGAATGCAGGAAAAACACTAACAGATGAACAAGTTGTTAT GGGCAATAAGCTCCTTGTATATATTAGCTGTTGTCTAGCAGGTCGTGCCTATCCCCTTGG TGACATCCCTGAAGATCTGGTTCCCTTGGTTAAAAACCAGGTTTTTGAATTTCTAATTCG CCTGCATTCAGCAGAGGCTTCTCCTGAGGAAGAAATCTATCCTTACATTCGGACTTTGCT ACATTTTGACACAAGAGAATTTCTCAATGTATTGGCACTGACTTTTGAAGATTTTAAAAA TGACAAGCAAGCTGTGGAATATCAACAGCGAATTGTGGATATTTTGTTGAAAGTTATGGT GGAGAATTCAGACTTTACCCCCTCACAAGTAGGATGTCTCTTTACCTTCCTTGCTCGGCA GCTTGCAAAGCCTGACAACACCTTGTTTGTAAACAGAACACTTTTTGATCAGGTCCTTGA ATTCCTTTGTAGTCCTGACGATGACTCCCGACACTCTGAAAGACAGCAGGTCCTTTTAGA ATTGCTGCAGGCTGGAGGCATAGTTCAATTTGAAGAGAGTCGACTCATCCGGATGGCAGA AAAAGCTGAGTTCTATCAAATTTGTGAATTTATGTATGAAAGAGAACACCAATATGACAA AATTATTGATTGCTACTTACGTGACCCTCTGCGAGAGGAAGAAGTCTTTAATTACATTCA CAATATCTTATCCATTCCCGGACACAGTGCAGAGGAGAAGCAGTCTGTATGGCAGAAAGC AATGGATCATATTGAGGAACTCGTGTCCCTGAAGCCTTGTAAAGCTGCGGAGCTGGTTGC CACCCACTTTTCTGGACATATTGAAACGGTCATTAAAAAACTTCAGAACCAGGTTTTGCT TTTCAAATTTTTGAGGAGTCTTCTTGACCCAAGGGAAGGTATTCATGTAAATCAAGAATT ACTGCAAATATCTCCTTGTATCACAGAGCAGTTCATTGAGCTGTTGTGTCAGTTCAACCC AACCCAAGTTATAGAGACTCTGCAAGTCCTTGAGTGCTACCGTCTGGAAGAAACTATTCA GATTACTCAGAAGTATCAACTTCATGAAGTCACCGCTTATCTATTGGAAAAGAAAGGAGA TATTCATGGTGCCTTCCTAATAATGTTAGAGAGACTACAAAGCAAACTTCAAGAGGTAAC ACATCAAGGTGAAAATACCAAAGAGGATCCCTCATTGAAGGATGTTGAAGATACTATGGT GGAGACCATTGCTCTTTGCCAGAGAAATTCACATAATTTGAACCAGCAGCAACGTGAGGC CCTTTGGTTTCCGTTATTGGAGGCAATGATGGCCCCTCAGAAGCTGTCCAGTTCAGCCAT TCCTCATCTACACTCTGAAGCTCTGAAGTCTTTGACCATGCAAGTTTTAAATAGCATGGC AGCATTTATTGCCCTTCCATCAATCTTGCAAAGAATCTTACAGGATCCAGTTTATGGAAA AGGAAAACTTGGAGAAATCCAGGGACTTATCTTGGGAATGTTAGATACCTTTAACTATGA ACAAACCCTGCTGGAAACAACAACCAGCCTTCTAAACCAAGATCTCCATTGGTCATTGTG TAACCTGAGAGCTTCGGTCACCAGAGGACTGAATCCCAAACAAGATTACTGCTCTATATG TTTGCAGCAGTACAAGAGACGCCAAGAAATGGCTGATGAAATAATTGTCTTTAGCTGTGG CCATTTGTATCACTCATTCTGCCTACAAAACAAAGAATGCACTGTGGAATTTGAGGGCCA AACAAGATGGACATGCTACAAATGCAGTTCAAGTAACAAAGTAGGAAAACTCAGTGAAAA TTCATCTGAAATTAAAAAGGGAAGGATAACCCCATCACAGGTAAAAATGTCTCCATCGTA TCATCAGTCCAAAGGGGATCCCACTGCTAAAAAGGGAACCTCAGAACCTGTTCTGGATCC ACAGCAAATCCAAGCATTTGATCAGCTTTGCCGTCTCTACCGAGGAAGCTCCAGGCTGGC TCTCCTCACGGAACTCTCCCAGAATCGCAGCAGCGAGAGCTATAGGCCATTCAGTGGCTC GCAGAGTGCTCCTGCTTTCAACAGCATCTTCCAGAATGAGAACTTCCAGCTGCAGCTCAT TCCTCCACCTGTGACTGAGGATTGATGACTCCATGGAGCCTGGCCCAGGAGAACCAGAGA TGATCCCGAGGCAGCTGGGGAGAGGCCCCGCCTCTGGTGGGCTTGGCCTCCACCACCTCC CACGCTTCTGAGAAGAGGTTCCAAATTGGGCTTCTGTGCCCAGAGCGTCCACAGCACCAT TCCCAGTGTAGACTCCCAGTCTTCTCCACATTGCTGTCATGGCGTCAGTTCACCAGACTC ATTGATTTTGTTTTGCTTGTTAAGCAAAGGAATGTCACATACCTCTGTCCAGCTTTTTAG GAAATACATTTCGCCTATTGCGACTTTTTCCATTTACCCTGAAGCCTAGAAAGTAGGTGG AACTCACACAAATGGCATTCCAGAGTCTGCCATACTCCGTCTCCTCCAGCTGCTGGATAA TACAGAGGAACTTCAACTTCTACAGGGAACAGTGGTTGGCCAGGCTGCAGTATAACTGAA GCATGCCTTGGAGAGAGCAGACACTGTGGGGGCCAGGGCCATCTCCCTTTAATGTGTTCA TGTTAAAACCTATTTGAGTGTAAGACTTGCCCTTTCTAACAATAAATGCTCCGTGTTTAA AAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001009921 |
| Insert Size | 5000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to contain 6bp deletion compared with NM_001009921.1. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001009921.1, NP_001009921.1 |
| RefSeq Size | 5009 bp |
| RefSeq ORF | 4287 bp |
| Locus ID | 23355 |
| UniProt ID | Q8N3P4 |
| Gene Summary | Plays a role in vesicle-mediated protein trafficking of the endocytic membrane transport pathway. Believed to act as a component of the putative CORVET endosomal tethering complexes which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed:25266290). Functions predominantly in APPL1-containing endosomes (PubMed:25266290).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (a). Variants 1, 3, 4, 5, and 6 all encode the same isoform (a). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224126 | VPS8 (Myc-DDK-tagged)-Human vacuolar protein sorting 8 homolog (S. cerevisiae) (VPS8), transcript variant 1 |
CNY 9768.00 |
|
| RC224126L3 | Lenti-ORF clone of VPS8 (Myc-DDK-tagged)-Human vacuolar protein sorting 8 homolog (S. cerevisiae) (VPS8), transcript variant 1 |
CNY 16150.00 |
|
| RC224126L4 | Lenti-ORF clone of VPS8 (mGFP-tagged)-Human vacuolar protein sorting 8 homolog (S. cerevisiae) (VPS8), transcript variant 1 |
CNY 16150.00 |
|
| RG224126 | VPS8 (tGFP-tagged) - Human vacuolar protein sorting 8 homolog (S. cerevisiae) (VPS8), transcript variant 1 |
CNY 15680.00 |
