PKD1L2 (NM_052892) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PC1L2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC305688 representing NM_052892. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGTGCAGTGGGCTTGGTACTCCTGGTGCTTGCTCTGAGGCTTAGGGCCACCACTGTTAAGCCAGAG GAAGGCAGCTTTTGCTCTAACAGCCAGGTGGCCTTCAGAGATGCTTGCTATGAATTTGTGCCACTCGGA CGCACCTTCCGTGATGCCCAGAGCTGGTGCGAGGGGCAAGGAGGCCATTTGGTCTTCATTCAGGACGAA GGCACCCAGTGGTTTCTGCAGAAGCACATCTCCCAGGACAGGGAATGGTGGATTGGACTCACGTGGAAC TTGGCACGGAATGGAACCACGGAGGGGCCGGGGACTTGGTTGGACACCTCCAATGTGACCTACAGCAAC TGGCATGGAGGGCAGGCCGCCGCTGCCCCTGACACCTGCGGCCACATCGGGAGAGGCCCTTCCTCTGAG TGGGTGACCTCGGACTGCGCCCAGACCTTCGCCTTCATGTGCGAGTTCCGGGTTGGCCAGAGCCTGGCC TGCGAGGGACTCAATGCCACCGTGCACTGTGGCTTGGGGCAGGTCATCCAGGTCCAGGATGCCGTCTAC GGGCGCCAGAATCCCCATTTCTGCACCCAGGATGCCGGGCGTCCTTCAGATCTGGAGCAGGGATGCAGC TGGGCCAACGTCAAGGAGGAGGTGGCAGGCCAGTGCCAGGAGCTACAGTCATGCCAGGTGGCAGCAGAT GAGACCTACTTTGGAAACCTGTGTCCGACTCAGGGCAGTTACCTGTGGGTGCAGTACCAGTGCCGGGAA GCCCTGCAGCTGATGGTGTCCAGTGAGAGTTTCATCTTTGACAATGTCACCATCTCCCTGACGTGGCTC CTCTCACCCTACATAGGAAACCTGTCCTGTATAATTAGTACAGGAGACAGCCACACTTTTGATCCCTAC AACCCGCCGAGTGTGTCCAGCAATGTGACCCACCAGTTCACATCTCCTGGGGAATTCACCGTGTTTGCT GAATGCACAACCAGTGAGTGGCATGTGACAGCTCAGAGGCAGGTGACCGTGCGAGACAAGATGGAGACG CTCAGTGTGACTGCATGCTCCGGCCTGTCCCAGTCAGGAGCCGGCCCTCTCTGCCAGGCTGTCTTTGGG GATCCTCTGTGGATTCAGGTGGAGCTTGATGGAGGAACAGGAGTGACTTACACTGTGCTTTTGGGTGAC ATAACCCTGGCCGAGTCCACCACCCAAAAGGGCTCGCTACCGTACAATCTGATCCTGGACAGAGAAACC CAGAAACTGATGGGCCCTGGGAGGCACCGCCTGGAGATCCAAGCCACCGGCAACACCACCACCTCTACA ATCTCCAGAAACATTACAGTCCACTTGGTGGAGCTGCTGTCAGGGCTGCAGGCCTCCTGGGCTTCTGAC CATTTGGAGCTTGGACAGGACCTATTGATCACCATCTCATTGGCTCAGGGCACCCCGGAAGAGCTGACC TTTGAGGTGGCTGGACTCAATGCAACCTTCTCCCACGAGCAAGTGAGCTTTGGAGAGCCATTTGGGATT TGCCGCCTGGCTGTTCCGGTTGAAGGCACCTTTCTGGTCACCATGCTGGTGAGGAATGCCTTCTCCAAC CTGAGTTTGGAAATCGGGAACATCACTATCACAGCCCCTTCCGGTCTCCAAGAACCATCTGGGATGAAT GCTGAAGGAAAGAGTAAAGATAAAGGCGATATGGAGGTGTACATCCAACCTGGTCCATATGTGGATCCT TTCACGACAGTGACCCTGGGCTGGCCAGACAATGACAAGGAGTTACGCTTCCAATGGTCATGTGGGTCT TGCTGGGCTCTGTGGAGCAGCTGTGTTGAGAGGCAGCTGCTTCGCACAGACCAGAGGGAGCTGGTGGTT CCAGCATCCTGCCTGCCGCCGCCTGACTCTGCTGTCACCCTGCGCCTGGCTGTTCTGAGAGGCCAAGAG CTGGAGAACAGGGCAGAGCAGTGCCTCTACGTGTCTGCGCCCTGGGAACTCAGGCCTCGAGTCAGCTGT GAGAGGAACTGCAGGCCAGTTAATGCCAGCAAAGACATTCTGCTCAGGGTCACCATGGGGGAGGACTCT CCAGTGGCTATGTTCAGCTGGTATTTGGACAACACCCCAACAGAGCAGGCTGAGCCCCTCCTGGATGCC TGCAGACTCAGAGGATTTTGGCCAAGGTCCTTAACCCTCCTCCAGAGCAACACCTCCACGTTGCTGTTG AACAGCTCGTTTCTGCAGTCCCGGGGAGAGGTCATCCGAATCAGAGCCACAGCACTGACCAGGCATGCC TATGGGGAGGACACCTATGTGATCAGCACTGTGCCTCCCCGTGAGGTGCCTGCCTGCACTATTGCCCCA GAGGAGGGCACCGTTCTGACGAGCTTTGCCATCTTCTGCAACGCCTCCACAGCCCTGGGACCCCTGGAG TTCTGCTTCTGTCTGGAATCAGGTTCCTGCCTACACTGTGGCCCTGAACCTGCCCTCCCATCAGTGTAT CTGCCACTTGGAGAGGAGAACAATGACTTTGTGCTGACAGTAGTTATTTCTGCCACCAATCGTGCAGGG GACACGCAGCAGACCCAGGCCATGGCTAAGGTGGCACTCGGAGACACATGTGTTGAGGATGTAGCATTC CAGGCTGCCGTGTCAGAGAAAATCCCCACAGCTCTGCAAGGCGAGGGTGGCCCCGAGCAGCTCCTCCAG CTGGCCAAGGCTGTGTCCTCCATGCTGAACCAAGAGCATGAAAGCCAGGGCTCAGGACAGTCACTGAGC ATAGACGTCAGACAGAAGGTCAGAGAGCATGTGCTGGGATCACTGTCTGCAGTCACCACCGGCTTGGAG GACGTGCAGAGGGTGCAGGAGCTGGCCGAGGTGCTGAGAGAGGTGACCTGCCGGAGTAAGGAACTCACA CCCTCGGCCCAGTGGGAGGCCAGCTTGGCTCTACAGCATGCCAGTGAGGCCCTGTTGACAGTGAGTGCC AAGGCCCGCCCTGAGGACCAGAGGCGCCAGGCAGCCACCAGGGACCTGTTTCAGGCTGTGGGCAGTGTG CTGGAAGCTTCCCTGAGCAACAGACCAGAAGAGCCTGCGGAGGCCAGCAGCAGCCAGATTGCCACAGTG CTGCGGCTGCTTCGAGTCATGGAGCATGTGCAGACCACCCTCCTGCTGGGAAAACTGCCAGGGGGCCTT CCAGCCATGCTGGCCACCCCCTCCATCTCTGTGTACACAAACAGAATACAACCCTGGAGTTGGCAAGGC TCCTCCCTGCGCCCTGATGCCGCAGACTCTGCAACCTTCATGCTGCCCGCTGCCTCCTCCCTCAGCTCT CTGGAGGGCGGCCAGGAGCCCGTGGATATAAAGATCATGAGTTTCCCAAAGAGCCCCTTTCCAGCCCGA AGCCACTTTGATGTCAGCGGGACTGTCGGTGGCCTCCGTGTGACCAGCCCTAGTGGTCAACTCATACCT GTGAAGAATCTGTCGGAGAATATCGAGATCCTGCTGCCCCGGCATTCACAAAGACACAGCCAGCCGACC GTGTTGAACCTGACCAGTCCTGAAGCTTTGTGGGTGAACGTGACTTCAGGGGAGGCAACCTTGGGGATC CAGCTGCACTGGAGACCAGACATTGCACTCACGCTTAGCCTGGGCTATGGCTACCACCCCAACAAGAGC AGCTACGATGCCCAAACTCACCTCGTACCAATGGTGGCTCCAGATGAGCTGCCCACGTGGATCCTGAGC CCACAGGACCTGCGTTTTGGAGAAGGGGTCTACTATTTGACTGTGGTCCCTGAGTCTGACCTGGAGCCA GCCCCCGGCAGGGACCTCACGGTTGGCATCACCACCTTCCTGTCTCACTGTGTGTTCTGGGATGAGGTC CAGGAGACTTGGGACGACTCAGGATGCCAGGTGGGGCCTCGGACCAGCCCCTACCAGACACACTGCCTC TGCAACCACCTCACTTTCTTCGGAAGCACGTTCCTGGTGATGTCCAATGCCATCAACATCCACCAGACT GCTGAGCTCTTTGCCACCTTTGAGGACAACCCTGTGGTCGTGACCACCGTGGGCTGCCTGTGTGTGGTC TACGTGCTGGTGGTGATCTGGGCGAGGAGGAAGGACGCTCAGGATCAGGCCAAGGTGAAGGTCACAGTG CTGGAAGACAATGATCCCTTTGCTCAGTACCACTACCTGGTGACAGTCTACACAGGACACCGACGAGGG GCAGCCACGTCCTCAAAGGTGACTGTCACCCTGTATGGCCTGGATGGAGAGAGAGAGCCCCACCACCTG GCTGATCCCGACACTCCGGTTTTTGAGCGAGGAGCAGTGGATGCCTTCCTCCTCTCCACCCTGTTCCCC CTGGGAGAACTGCGGAGCCTCCGGCTGTGGCATGACAACTCAGGGGACCGGCCATCGTGGTATGTGAGC CGGGTGCTGGTCTATGACCTGGTGATGGACCGGAAGTGGTATTTCCTGTGCAACTCCTGGCTATCCATC AATGTTGGAGATTGCGTCCTCGATAAGGTGTTTCCTGTGGCCACGGAGCAGGACAGAAAACAATTCAGC CACCTGTTTTTCATGAAGACTTCCGCGGGCTTCCAGGATGGACACATCTGGTATTCGATCTTCAGCCGC TGCGCTCGCAGCAGCTTCACCCGCGTCCAGAGGGTGTCCTGCTGCTTCTCCCTGCTGCTGTGCACCATG CTGACCAGCATCATGTTCTGGGGGGTCCCCAAGGACCCAGCTGAGCAAAAGATGGACTTGGGTAAAATT GAATTCACCTGGCAGGAGGTGATGATTGGCCTGGAGAGCTCCATCCTCATGTTCCCCATCAACCTCCTG ATTGTTCAGATCTTTCAGAACACCCGTCCCCGGGTCGCGAAGGAGCAGAACACTGGAAAATGGGACCGG GGGTCCCCCAACCTGACTCCCTCCCCACAGCCCATGGAGGACGGCCTTCTGACACCTGAGGCAGTGACC AAGGATGTGTCAAGAATCGTCAGCTCCCTCTTCAAAGCTCTCAAGGTGCCATCCCCCGCCTTGGGCTGG GACTCAGTGAACTTGATGGACATCAACAGTCTCCTCGCCTTGGTGGAAGATGTCATTTATCCACAGAAC ACATCAGGGCAGGTGTTCTGGGAGGAAGCCAAAAAGAGAGAGGACCCTGTAACACTCACTTTGGGGTCA TCAGAAATGAAAGAGAAATCACAGTGTCCCAAGCCCAAGGCGGCACGGAGTGGCCCCTGGAAGGACAGC GCCTACAGGCAGTGTCTGTACCTTCAGCTGGAACACGTGGAGCAAGAGCTGCGGCTGGTGGGGCCCCGA GGCTTCTCCCAGCCCCACAGCCATGCCCAGGCCCTCAGGCAGCTGCAGACCCTGAAGGGCGGCCTGGGG GTACAGCCGGGCACCTGGGCCCCTGCACATGCCAGCGCTCTTCAGGTGAGCAAACCCCCTCAAGGCCTG CCCTGGTGGTGCATCCTGGTGGGCTGGCTCCTGGTAGCGGCCACCAGTGGCGTGGCGGCCTTCTTCACC ATGCTCTACGGCCTGCACTACGGGAGGGCCAGCTCCCTCAGGTGGCTCATCTCCATGGCTGTCTCCTTC GTGGAGAGCATGTTCGTCACCCAGCCCCTGAAGGTGCTGGGATTCGCTGCTTTCTTTGCACTGGTCTTG AAGAGAGTGGACGATGAGGAGGATACTGTGGCCCCGCTGCCAGGACATCTGTTGGGCCCAGACCCCTAT GCCTTGTTCCGAGCACGAAGAAACAGCAGCAGGGATGTCTACCAGCCACCTCTCACCGCTGCCATTGAG AAGATGAAAACCACCCACCTCAAGGAACAGAAAGCATTTGCCCTCATCAGAGAAATCCTGGCATACTTG GGCTTCCTGTGGATGCTACTGCTCGTGGCCTACGGGCAGAGGGACCCCAGCGCCTACCACCTCAACAGA CACCTCCAGCACAGCTTCACCAGGGGCTTTTCAGGTGTGCTCGGCTTCCGAGAGTTCTTCAAGTGGGCC AACACCACCCTCGTGAGTAACCTGTATGGTCACCCCCCAGGCTTTATCACTGATGGGAACTCCAAGCTG GTTGGCAGTGCCCAGATTCGTCAAGTGAGGGTCCAGGAAAGCTCTTGCCCTCTTGCCCAGCAGCCGCAG GCATATCTCAACGGATGCCGTGCACCATATTCCCTGGATGCTGAAGACATGGCAGACTATGGGGAAGGC TGGAATGCCACCACCCTCAGTGAATGGCAGTACCAGAGCCAGGACCAACGTCAAGGGTATCCCATCTGG GGCAAACTCACTGTGTACCGGGGAGGAGGCTACGTGGTCCCCTTGGGGACTGATCGCCAAAGCACGTCA AGAATTCTCCGCTATCTCTTTGACAACACCTGGCTGGACGCCCTGACCAGAGCTGTGTTTGTGGAGTCC ACTGTCTACAACGCCAACGTCAACCTGTTCTGCATTGTCACGCTGACGCTAGAGACCAGCGCTCTGGGC ACCTTTTTTACGCACGCGGCCCTGCAGAGCCTCCGCCTGTACCCCTTCACCGACGGCTGGCACCCCTTC GTGGTAGCGGCAGAGCTCATCTACTTCCTCTTCCTCCTCTACTACATGGTGGTGCAGGGCAAGCGCATG AGTAAAGAGACGTGGGGCTATTTCTGCAGCAAGTGGAACCTTCTGGAGCTGGCCATCATCCTGGCCAGC TGGAGCGCCCTGGCGGTGTTTGTGAAGAGGGCTGTCCTGGCCGAAAGGGACCTCCAGCGCTGCCGGAAC CACAGGGAGGAAGGCATCAGCTTCAGTGAGACAGCAGCAGCCGATGCCGCCCTTGGCTACATCATTGCC TTCCTGGTACTCCTGTCCACAGTGAAGCTTTGGCATCTGCTCAGGTTGAATCCCAAAATGAACATGATC ACGGCAGCCCTACGCCGTGCCTGGGGCGACATTTCAGGCTTTATGATTGTCATCCTTACCATGCTCCTG GCTTACTCCATCGCGTCAAACTTGATATTTGGTTGGAAACTCCGTTCCTACAAAACCCTCTTTGATGCG GCGGAGACGATGGTCAGCCTTCAGCTGGGAATCTTCAACTACGAGGAGGTCCTGGACTATAGCCCAGTG CTTGGCTCCTTCCTCATTGGATCCTGCATTGTTTTTATGACATTTGTGGTGCTGAACCTGTTTATCTCT GTCATCCTGGTGGCCTTCAGTGAGGAGCAAAAATACTATCAGCTGTCGGAGGAAGGGGAGATCGTAGAT TTGCTGCTGATGAAAATACTCAGTTTCCTGGGCATTAAGTCTAAGAGAGAGGAGCCTGGAAGCAGCAGG GAGCAGCCTGGGTCTCTGTCCCAGACTCGCCACTCTCGACCAGCACAAGCTTTGCCCAAGGACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_052892
Insert Size	7380 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_052892.3
RefSeq Size	7624 bp
RefSeq ORF	7380 bp
Locus ID	114780
UniProt ID	Q7Z442
Protein Families	Druggable Genome
MW	272.5 kDa
Gene Summary	This gene encodes a member of the polycystin protein family. The encoded protein contains 11 transmembrane domains, a latrophilin/CL-1-like GPCR proteolytic site (GPS) domain, and a polycystin-1, lipoxygenase, alpha-toxin (PLAT) domain. This protein may function as a component of cation channel pores. This gene appears to be a polymorphic pseudogene in humans, and the reference genome encodes a non-functional allele. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2019] Transcript Variant: This variant (1, also known as long form) represents the functional allele that is absent from the reference genome but present in some human individuals. This variant is the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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