COL24A1 (NM_152890) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC306518 representing NM_152890. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCATTTAAGAGCCCACAGAACAAGGCGTGGAAAAGTCTCCCCCACGGCAAAAACGAAATCACTTCTT CATTTTATTGTACTATGTGTGGCTGGGGTGGTTGTTCATGCACAAGAACAAGGCATAGATATTCTTCAT CAACTAGGCCTTGGAGGCAAAGACGTAAGACACTCATCACCAGCGACTGCTGTACCATCAGCATCTACA CCGTTACCTCAGGGGGTCCATTTAACAGAATCAGGAGTCATTTTTAAAAATGATGCTTATATCGAGACA CCTTTCGTGAAAATTTTACCAGTCAACTTGGGGCAGCCGTTTACAATATTAACTGGGTTACAGTCACAT CGGGTGAACAATGCATTTCTCTTCAGCATTAGAAATAAAAATAGACTGCAATTAGGAGTACAATTACTA CCTAAAAAATTAGTAGTACACATTAGAGGAAAGCAGCCTGCAGTTTTCAACTACAGTGTTCATGATGAG CAATGGCACTCATTTGCCATTACTATTAGAAACCAAAGTGTCTCAATGTTTGTTGAGTGTGGAAAGAAA TATTTTAGCACAGAGACTATTCCAGAAGTTCAGACCTTTGATTCTAATAGTGTGTTTACTTTAGGAAGT ATGAATAATAATTCTATCCATTTTGAAGGAATAGTATGTCAGTTAGATATTATTCCTTCTGCAGAAGCA TCTGCAGACTACTGCAGATATGTGAAACAGCAGTGTCGCCAAGCAGACAAATACCAACCTGAAACAAGC ATTCCTTGTACAACTCTCATACCAACAAAGATACCGGAACACTCTCCCCCGCCCAAACTATTTGCTGAA AAAGTACTGTCAGAGGATACATTTACTGAAGGCAAAAGCATTCCAAATATCATAAAAAATGATTCTGAA ACCGTGTATAAAAGACAAGAACACCAGATATCAAGATCTCAGTTATCTTCTCTTCAGTCAGGAAATGTC TCTGCTGTGGATCTCACAAACCATGGGATTCAGGCCAAAGAAATGATCACTGAGGAAGATACTCAGACA AATTTCAGCCTGTCAGTGACCACTCATCGCATCAGTGAGGCAAAAATGAATACCAAAGAGAAATTTAGC TCTCTCCTAAACATGTCTGACAATATCACACAACATGATGATAGAGTAACTGGTCTGTCACTGTTTAAG AAGATGCCATCTATTCTTCCACAAATTAAACAAGATACAATTACTAATCTCAAGAAGGCTATCACAGCA AATCTACACACTAACGAACTCATGGAAATGCAACCAATTTTAAACACAAGCTTGCATAGAGTGACAAAT GAGCCATCTGTGGATAATCACCTTGATCTAAGGAAAGAAGGTGAATTTTATCCTGATGCTACTTATCCC ATCGAAAATAGCTATGAAACTGAGCTTTATGATTATTATTATTATGAGGATCTAAATACAATGCTTGAA ATGGAGTATCTGAGAGGGCCAAAAGGAGACACTGGACCTCCCGGTCCACCTGGTCCAGCAGGTATCCCA GGTCCGTCAGGGAAGAGAGGTCCACGGGGCATACCAGGGCCACATGGAAATCCTGGTTTACCTGGATTA CCTGGTCCAAAGGGCCCCAAAGGAGATCCAGGATTTTCCCCAGGTCAACCTGTTCCTGGAGAAAAGGGT GATCAAGGCCTTTCTGGATTAATGGGCCCCCCTGGTATGCAAGGTGATAAGGGTCTCAAAGGACATCCT GGACTCCCAGGACTTCCAGGTGAACAAGGAATTCCAGGATTTGCTGGTAATATTGGTTCACCCGGTTAC CCTGGCAGGCAGGGTTTAGCTGGACCAGAAGGTAATCCAGGTCCTAAAGGTGCACAAGGTTTTATTGGC TCTCCTGGAGAAGCGGGACAACTGGGACCTGAAGGAGAACGGGGCATTCCTGGGATCCGTGGAAAGAAG GGTTTTAAGGGGAGACAGGGTTTTCCAGGTGACTTTGGAGACAGAGGCCCTGCTGGTCTTGATGGCAGT CCTGGACTTGTAGGTGGCACTGGTCCTCCGGGGTTTCCTGGGCTTAGAGGCAGTGTTGGCCCTGTGGGA CCAATTGGACCTGCTGGAATTCCTGGCCCAATGGGCCTTTCAGGGAATAAAGGACTACCTGGAATCAAA GGTGATAAGGGTGAACAAGGCACAGCAGGAGAGCTAGGAGAACCCGGGTATCCTGGAGACAAGGGTGCT GTTGGTTTACCAGGACCACCAGGGATGAGAGGAAAGTCAGGGCCTTCAGGCCAAACAGGTGACCCAGGA CTCCAAGGACCATCTGGCCCTCCAGGACCAGAGGGTTTTCCAGGAGATATTGGGATTCCTGGACAAAAC GGCCCTGAAGGACCAAAGGGACTCCTTGGAAATAGAGGACCTCCTGGACCTCCTGGTCTCAAAGGAACT CAGGGAGAAGAAGGACCAATTGGAGCCTTTGGGGAACTGGGGCCAAGAGGAAAACCAGGTCAAAAGGGG TATGCAGGTGAACCAGGACCAGAAGGCTTAAAGGGAGAAGTAGGAGATCAAGGAAATATTGGAAAAATT GGTGAAACAGGACCTGTTGGCTTACCTGGAGAAGTTGGGATGACTGGAAGCATTGGCGAAAAGGGAGAA CGTGGAAGTCCAGGCCCACTAGGTCCGCAGGGAGAAAAAGGTGTTATGGGATATCCAGGTCCTCCTGGG GTTCCAGGACCTATCGGTCCATTGGGATTACCTGGTCATGTGGGGGCAAGAGGACCACCTGGGAGTCAA GGTCCTAAAGGACAAAGAGGATCAAGAGGACCAGATGGTCTCTTAGGGGAACAAGGTATACAAGGTGCC AAGGGTGAAAAAGGAGATCAAGGAAAAAGAGGGCCTCATGGTCTTATTGGTAAGACTGGAAACCCTGGA GAAAGAGGATTTCAAGGGAAACCAGGTTTACAGGGATTGCCTGGAAGTACAGGTGACAGAGGACTTCCA GGAGAGCCAGGACTGCGAGGACTGCAAGGTGATGTTGGACCTCCTGGAGAAATGGGCATGGAGGGACCT CCAGGCACTGAGGGAGAGTCTGGTCTGCAAGGTGAACCAGGTGCAAAGGGAGATGTTGGAACTGCTGGC AGTGTTGGAGGAACTGGGGAACCAGGTTTACGGGGTGAACCAGGAGCTCCTGGAGAAGAAGGTCTCCAG GGAAAAGATGGGTTAAAGGGAGTACCAGGAGGAAGGGGACTTCCTGGAGAGGATGGAGAAAAAGGAGAG ATGGGCTTACCAGGAATTATAGGACCCTTGGGTAGATCAGGCCAAACAGGCCTTCCGGGACCTGAAGGA ATTGTGGGAATTCCAGGGCAAAGAGGTCGTCCAGGAAAAAAGGGTGATAAAGGACAAATAGGACCCACA GGAGAAGTTGGAAGCAGAGGTCCTCCTGGAAAAATTGGGAAAAGTGGTCCTAAGGGTGCCAGAGGAACT AGAGGTGCTGTGGGACATTTAGGATTGATGGGACCTGATGGAGAACCAGGAATTCCAGGGTACAGGGGC CATCAGGGCCAACCAGGACCCTCTGGATTGCCAGGACCTAAAGGAGAAAAGGGCTACCCAGGAGAAGAT AGCACAGTCCTAGGACCACCTGGGCCTCGAGGTGAACCAGGTCCAGTGGGGGACCAAGGAGAGAGAGGA GAGCCTGGAGCAGAGGGATATAAGGGCCATGTGGGTGTACCAGGACTAAGAGGTGCCACTGGACAACAA GGACCCCCAGGCGAACCAGGTGACCAGGGTGAACAAGGACTAAAAGGAGAGAGAGGATCTGAAGGTAAT AAGGGGAAAAAAGGAGCTCCTGGTCCTTCTGGGAAACCTGGGATTCCTGGACTTCAAGGCCTACTTGGG CCAAAAGGCATACAAGGATACCATGGAGCAGATGGCATTTCAGGAAACCCTGGAAAAATTGGGCCACCA GGAAAACAGGGACTTCCTGGCATCAGAGGCGGCCCAGGAAGAACAGGTCTTGCTGGGGCTCCAGGTCCT CCAGGAGTAAAGGGTTCATCAGGCTTGCCAGGAAGCCCAGGAATTCAAGGCCCAAAGGGTGAACAAGGG CTACCTGGTCAACCTGGAATTCAAGGTAAAAGAGGTCACCGAGGAGCACAAGGTGATCAAGGACCATGT GGAGACCCTGGCCTGAAAGGGCAGCCTGGAGAATATGGTGTTCAAGGTTTGACAGGTTTCCAAGGATTC CCAGGCCCTAAAGGTCCTGAAGGGGATGCTGGCATTGTTGGGATATCAGGTCCTAAAGGTCCTATTGGA CACAGAGGAAACACTGGTCCCCTTGGCAGAGAAGGTATAATAGGCCCAACAGGTAGAACTGGACCCAGA GGTGAAAAGGGCTTTAGAGGTGAAACTGGTCCTCAAGGACCAAGAGGTCAACCAGGGCCTCCAGGTCCA CCTGGAGCACCAGGCCCAAGAAAGCAAATGGATATCAATGCTGCTATTCAAGCCTTGATTGAATCAAAT ACTGCCCTACAGATGGAGAGCTACCAGAATACTGAAGTGACTTTAATTGACCACAGTGAAGAGATATTC AAAACCCTGAACTACCTTAGCAATTTATTGCACAGCATCAAGAATCCTCTTGGCACACGAGATAACCCA GCACGAATCTGCAAAGATTTACTTAACTGTGAACAAAAAGTATCAGATGGAAAATACTGGATTGACCCA AATCTTGGCTGTCCTTCAGATGCCATTGAGGTTTTCTGCAATTTCAGTGCTGGTGGCCAGACATGCTTA CCTCCTGTTTCTGTAACAAAGTTGGAGTTTGGAGTTGGGAAAGTCCAGATGAACTTCCTTCATTTACTG AGTTCGGAAGCCACCCATATCATCACCATTCACTGTCTAAACACCCCAAGGTGGACAAGCACACAAACA AGTGGCCCAGGATTGCCTATTGGTTTCAAGGGATGGAATGGCCAGATTTTTAAAGTAAACACTCTACTT GAACCTAAAGTGCTTTCAGATGACTGCAAGATTCAAGATGGCAGCTGGCATAAGGCAACATTTCTTTTT CACACCCAGGAACCTAATCAACTTCCAGTGATTGAAGTACAAAAACTTCCTCATCTCAAAACTGAACGA AAGTATTACATTGACAGCAGTTCTGTATGCTTTCTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_152890
Insert Size	5145 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_152890.5
RefSeq Size	6500 bp
RefSeq ORF	5145 bp
Locus ID	255631
UniProt ID	Q17RW2
Protein Families	Transmembrane
MW	175.5 kDa
Gene Summary	This gene is a member of the collagen gene family and is thought to regulate type I collagen fibrillogenesis during fetal development. [provided by RefSeq, Mar 2017] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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