RNF70 (PJA1) (NM_001032396) Human Untagged Clone
CAT＃: SC321968
PJA1 (untagged)-Human praja ring finger 1 (PJA1), transcript variant 2
CNY 4384.00
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PRAJA1; RNF70 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for SC321968
GGCGGTCGGAGATCCGAGAGCTCTGCTTCTCTGAAAAGCGTGTGGCTTTCTGGACTTGGT
CTACACCTGACAGTCACTGGAATGCTACTCCAGGCCTTAGAAGAAAGTGAAACTAATTTT AAAGGCCCCTTATTCTTTCTGTTACCTGCCTGCTGAAGTGAAAATAAATCTCCCTGCTCC TTTATTCTAAACTGATTTTACTTTCCCATCTCAAAAACGTGTGCAGGGAGCAGGAGCCCT CCTACCCGAGCCCCAGCTGTATTGGAAACCCTCCTAAGTCAGAGTGATGGGTCAGGAATC TAGCAAGCCTGTATGGCCCAATCCAACAGGAGGGTATCAGTCCAATACAGGTAGGAGGTA TGGAAGAAGGCATGCTTATGTCAGTTTCAGGCCACCCACGAGCCAGCGGGAAAGGATTGC CAGCCAGAGAAAGACGAACTCCGAAGTCCCAATGCACAGATCAGCCCCCAGTCAAACCAC CAAGAGGAGCCGATCGCCATTTTCCACTACTCGTCGTAGTTGGGACGACAGCGAGAGTTC GGGAACCAACCTGAATATTGATAATGAGGACTATTCCAGGTATCCGCCAAGAGAGTACAG AGCTTCGGGTAGCAGAAGAGGAATGGCCTATGGACATATTGACTCTTATGGGGCAGATGA TAGTGAGGAGGAGGGGGCTGGGCCTGTTGAGCGACCGCCAGTGAGAGGGAAAACTGGCAA GTTTAAAGATGATAAGCTGTATGACCCAGAGAAAGGGGCAAGGTCTTTGGCTGGGCCACC TCCACATTTCTCTAGTTTTAGCCGTGATGTGAGAGAGGAGCGAGACAAGTTAGACCCAGT CCCTGCAGCAAGATGCTCAGCTAGCAGAGCTGACTTCCTGCCACAAAGTAGTGTGGCCTC ACAGTCGTCTTCTGAAGGCAAGCTGGCTACAAAAGGTGACAGCTCGGAGAGGGAGAGAAG GGAGCAAAATTTACCTGCACGTCCCAGCAGGGCTCCTGTGAGTATTTGTGGTGGTGGGGA AAACACCTCAAAGAGTGCAGAGGAACCTGTGGTCAGGCCCAAAATCAGAAACCTGGCAAG TCCAAACTGCGTGAAACCAAAAATTTTTTTTGATACTGATGATGATGACGATATGCCACA CAGTACTTCCAGGTGGAGGGATACCGCCAATGACAATGAGGGCCACTCGGATGGCCTGGC AAGAAGAGGGAGAGGCGAGAGTTCAAGTGGCTATCCCGAGCCAAAGTACCCTGAAGACAA ACGGGAAGCGAGGAGTGACCAAGTGAAACCAGAAAAGGTGCCGAGACGACGACGCACCAT GGCCGACCCTGACTTCTGGACGCACAGTGATGATTACTACAAATACTGCGACGAAGACTC TGACAGTGACAAAGAGTGGATTGCTGCTCTGCGTCGGAAATATCGAAGCCGAGAGCAAAC CCTGTCCTCCAGTGGCGAAAGCTGGGAGACTCTGCCGGGGAAAGAAGAGCGGGAACCTCC ACAGGCTAAGGTGAGTGCCAGCACTGGCACCAGCCCTGGCCCCGGTGCTAGTGCCAGTGC CGGGGCTGGCGCCGGGGCCAGTGCTGGCAGCAATGGCAGCAATTACCTTGAAGAAGTTCG AGAACCATCTCTTCAGGAAGAGCAGGCATCCCTGGAAGAAGGAGAAATTCCTTGGCTCCA GTACCATGAGAATGACAGTAGCAGTGAGGGGGATAATGATTCTGGTCACGAGTTGATGCA ACCTGGGGTATTCATGCTGGATGGAAACAACAACCTTGAAGATGACTCCAGTGTGAGCGA AGACCTAGAAGTGGATTGGAGCCTCTTTGATGGATTTGCAGATGGGTTAGGAGTGGCTGA AGCCATTTCCTATGTGGACCCTCAGTTCCTCACCTACATGGCACTTGAAGAACGCCTGGC CCAGGCAATGGAAACTGCCCTTGCGCACTTGGAGTCTCTCGCAGTGGATGTAGAGGTGGC CAATCCACCAGCAAGCAAGGAGAGCATTGACGCTCTTCCCGAGATCCTGGTCACTGAAGA TCATGGCGCAGTTGGTCAGGAGATGTGCTGCCCCATCTGCTGTAGCGAATATGTGAAGGG GGAGGTGGCAACTGAGCTGCCGTGCCACCACTATTTCCACAAGCCGTGTGTGTCCATCTG GCTTCAGAAGTCAGGCACCTGCCCCGTGTGCCGCTGCATGTTCCCTCCCCCACTCTAAAG ACCAAGGCCGTTTACTCCTGGTCTGATTATTTTCCCCATCTGAAATCCACAATACTGCAG GAGCCCTCTTGAAATTAACAATGGAAATAAAACCAATCAGTCAGTTAGCCTAAACCTATT GATTCCTCGTGATTATTTCCAATGTGAAAACAGTTGTGTATGATTGCATTAAAAATCATA TCATCTTTTAGAGGTTAGAAAAGGGAAAACTAAACTTTCTAAATGCTACTTGAGATTGCA GTAAGAAGATACGTTTTCTAACCTGAAAGTTAAATCGCATTTGTTTTCTTCAGTAGAATG GGAATGTGTTGCTGTTACTTGTAATGTCAAGTTTATCTGTTAAATATGTCCAAAAGGCAA AATCATTTTTGTTGCATGTTATGGGTCGATGTTCCTGTAATTGCAGTGCCGTAAAAGCTT ATTAAAGTTGTTCTTTTGGTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_001032396 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001032396.1, NP_001027568.1 |
| RefSeq Size | 2402 bp |
| RefSeq ORF | 1767 bp |
| Locus ID | 64219 |
| UniProt ID | Q8NG27 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes an enzyme that has E2-dependent E3 ubiquitin-protein ligase activity. This enzyme belongs to a class of ubiquitin ligases that include a RING finger motif, and it can interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene is located in an area of chromosome X where several X-linked cognitive disability disorders have been associated, and it has also been found as part of a contiguous gene deletion associated with craniofrontonasal syndrome, though a direct link to any disorder has yet to be demonstrated. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010] Transcript Variant: This variant (2, also known as PJA1beta) lacks a portion of the 5' UTR and 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209912 | PJA1 (Myc-DDK-tagged)-Human praja ring finger 1 (PJA1), transcript variant 2 |
CNY 6460.00 |
|
| RC209912L3 | Lenti-ORF clone of PJA1 (Myc-DDK-tagged)-Human praja ring finger 1 (PJA1), transcript variant 2 |
CNY 8360.00 |
|
| RC209912L4 | Lenti-ORF clone of PJA1 (mGFP-tagged)-Human praja ring finger 1 (PJA1), transcript variant 2 |
CNY 8360.00 |
|
| RG209912 | PJA1 (tGFP-tagged) - Human praja ring finger 1 (PJA1), transcript variant 2 |
CNY 7130.00 |
