GPBP1 (NM_001127236) Human Untagged Clone
CAT＃: SC323094
GPBP1 (untagged)-Human GC-rich promoter binding protein 1 (GPBP1), transcript variant 2
CNY 7700.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GPBP; SSH6; VASCULIN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC323094 representing NM_001127236.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGCAGCATGACTTTGCTCCAGCCTGGCTTAATTTCCCTACTCCACCATCATCAACAAAGGTCCTG ACCAGCAGTTTGAAATCGTCATTGAATTTTGAGAAGCATTCTGAAAACTTTGCATGGACAGAGAATCGT TATGATGTGAACCGTCGACGACACAACTCTTCAGATGGCTTTGATTCTGCTATTGGGCGTCCTAATGGA GGTAACTTTGGAAGGAAAGAAAAAAATGGATGGCGTACACATGGAAGAAATGGTACAGAAAACATAAAT CATCGAGGTGGATACCATGGTGGAAGTTCCCGTTCTCGTAGCAGTATTTTCCATGCAGGAAAAAGCCAA GGACTACATGAAAACAACATACCTGACAATGAAACCGGGAGGAAAGAAGACAAGAGAGAACGCAAACAG TTTGAAGCTGAGGATTTTCCGTCTTTAAATCCTGAGTATGAGAGAGAACCAAATCACAATAAGTCTTTA GCTGCAGGTGTGTGGGAATATCCTCCGAATCCTAAATCTAGAGCTCCAAGGATGCTGGTCATTAAGAAA GGTAATACAAAAGACTTACAGCTATCTGGATTCCCAGTAGTAGGAAATCTTCCGTCACAGCCAGTTAAG AATGGAACTGGTCCAAGTGTTTATAAAGGTTTAGTCCCTAAACCTGCTGCTCCACCTACAAAACCTACA CAATGGAAAAGCCAAACAAAAGAAAATAAAGTTGGAACTTCTTTCCCTCATGAGTCCACATTTGGCGTT GGCAACTTTAATGCTTTTAAATCAACTGCCAAGAACTTTAGTCCATCTACAAATTCAGTGAAAGAGTGT AATCGCTCAAATTCCTCTTCTCCTGTTGACAAACTTAATCAGCAGCCTCGTCTAACCAAACTGACACGA ATGCGCACTGATAAGAAGAGTGAATTTTTGAAAGCATTGAAAAGAGACAGAGTAGAAGAGGAACATGAA GATGAAAGCCGTGCTGGCTCAGAGAAGGATGACGACTCATTTAATTTACATAACAGCAATAGTACTCAC CAAGAAAGGGATATAAACCGAAACTTCGATGAAAATGAAATTCCTCAAGAGAATGGCAATGCCTCAGTG ATTTCCCAGCAGATCATTCGGTCTTCAACCTTCCCACAAACTGATGTTCTTTCAAGTTCACTTGAGGCA GAACACAGATTGTTAAAGGAAATGGGCTGGCAGGAAGACAGTGAAAATGATGAAACATGTGCTCCCTTA ACTGAGGATGAAATGAGAGAATTCCAAGTTATTAGTGAACAGTTACAGAAGAATGGTCTGAGAAAAAAT GGTATTTTGAAAAATGGCTTGATCTGTGACTTCAAGTTTGGACCGTGGAAGAACAGCACTTTCAAACCC ACAACTGAGAATGATGACACAGAGACAAGTAGCAGTGATACATCAGATGACGACGATGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001127236 |
| Insert Size | 1443 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001127236.2 |
| RefSeq Size | 3437 bp |
| RefSeq ORF | 1443 bp |
| Locus ID | 65056 |
| UniProt ID | Q86WP2 |
| MW | 54.1 kDa |
| Gene Summary | This gene was originally isolated by subtractive hybridization of cDNAs expressed in atherosclerotic plaques with a thrombus, and was found to be expressed only in vascular smooth muscle cells. However, a shorter splice variant was found to be more ubiquitously expressed. This protein is suggested to play a role in the development of atherosclerosis. Studies in mice suggest that it may also function as a GC-rich promoter-specific trans-activating transcription factor. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011] Transcript Variant: This variant (2) contains an additional in-frame coding exon compared to variant 1, resulting in a longer isoform (2) with a 7 aa peptide fragment not found in isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225814 | GPBP1 (Myc-DDK-tagged)-Human GC-rich promoter binding protein 1 (GPBP1), transcript variant 2 |
CNY 3656.00 |
|
| RC225814L3 | Lenti-ORF clone of GPBP1 (Myc-DDK-tagged)-Human GC-rich promoter binding protein 1 (GPBP1), transcript variant 2 |
CNY 5890.00 |
|
| RC225814L4 | Lenti-ORF clone of GPBP1 (mGFP-tagged)-Human GC-rich promoter binding protein 1 (GPBP1), transcript variant 2 |
CNY 5890.00 |
|
| RG225814 | GPBP1 (tGFP-tagged) - Human GC-rich promoter binding protein 1 (GPBP1), transcript variant 2 |
CNY 4370.00 |
