NBPF6 (NM_001143987) Human Untagged Clone

CAT#: SC326158

NBPF6 (untagged)-Human neuroblastoma breakpoint family, member 6 (NBPF6), transcript variant 1



  "NM_001143987" in other vectors (4)

CNY 12920.00


货期*
4周

规格
    • 10 ug

Product images

推荐一起购买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

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Rabbit Polyclonal Anti-NBPF6 Antibody
    • 100 ul

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC326158 representing NM_001143987.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGTGGTATCTGCCGACCCTTTGTCCAGCGAGAGGGCAGAGATGAACATCCTAGAAATCAACCAGGAA
TTGCGCTCGCAGCTGGCAGAGAGCAATCAGCAGTTCCGAGACCTCAAAGAGAAATTCCTTATAACTCAA
GCTACTGCCTACTCCCTGGCCAACCAGCTGAAGAAATACAAGTGTGAAGAGTACAAAGACATCATAGAC
TCTGTGCTGAGGGATGAACTGCAGTCCATGGAGAAGCTGGCAGAGAAGCTCAGGCAAGCTGAGGAGCTC
AGGCAGTATAAAGCCCTGGTTCACTCTCAGGCAAAAGAGCTGACCCAGTTACGGGAGAAGTTACGGGAA
GGGAGAGATGCCTCCCGCTGGCTGAACAAGCATCTGAAAACCCTCCTCACTCCTGATGACCCTGACAAG
TCCCAGGGTCAGGACCTCCGAGAGCAGCTGGCTGAGGGGCACAGGCTGGCAGAGCACCTTGTTCACAAG
CTGAGCCCAGAAAATGATGAAGATGAAGATGAGGATGAAGACGACAAAGACGAGGAGGTTGAGAAAGTA
CAGGAATCACCTGCCCCCAGAGAGGTGCAGAAGACTGAAGAAAAGGAAGTCCCTCAGGACTCACTGGAG
GAATGTGCTGTCACTTGTTCAAATAGTCACAACCCTTCTAACTCCAACCAGCCTCACAGGAGCACCAAA
ATCACATTTAAGGAACACGAAGTCGACTCTGCTCTGGTTGTAGAGAGTGAACACCCTCATGATGAAGAG
GAGGAAGCTCTAAACATTCCCCCAGAAAATCAAAATGACCATGAGGAGGAGGAGGGGAAAGCGCCAGTG
CCCCCCAGGAACCGACAATCACTTGAACCAGGTGAACTGACCAACCTCAGGGATTTCCTGATCTCACCT
GTGGTCTCCCATGTGGCTAATCCAGGACACCATGACAAGTCCAACTCTTACCGGCATCGTGAAGTCTCT
TTCTTGGCATTGGATGAACAGAAAGTTTGCTCCGCTCAGGATGTTGCCAGGGATTACTCCAATCCCAAA
TGGGATGAAACCTCACTTGGCTTCCTCGAAAAGCAAAGTGATCTTGAAGAGGTGAAAGGACAAGAAACA
GTTGCTCCCAGGCTCAGCAGGGGACCGCTGAGAGTGGACAAGCATGAAATCCCCCAGGAGTCACTGGAT
GGATGTTGCTTGACTCCTTCCATCCTTCCTGACCTGACTCCCTCCTACCACCCTTATTGGAGCACTTTG
TACTCTTTTGAAGACAAGCAAGTCAGCTTGGCTCTTGTAGACAAAATTAAAAAGGATCAAGAGGAGATA
GAAGACCAAAGCCCACCATGCCCCAGGCTCAGCCAGGAGCTGCCAGAGGTGAAGGAGCAGGAAGTCCCA
GAGGACTCTGTGAATGAAGTTTACTTGACTCCCTCAGTTCACCATGACGTGTCTGACTGCCACCAGCCT
TATAGCAGCACCTTGTCCTCATTGGAGGATCAGCTTGCCTGCTCTGCTCTGGATGTAGCCTCCCCCACC
GAGGCGGCCTGTCCCCAAGGGACTTGGAGTGGAGACTTGAGCCACCACCGGTCAGAGGTTCAAATTTCA
CAGGCACAGCTGGAACCAAGCACCCTGGTGCCCAGTTGTCTGCGACTACAGCTGGATCAAGGGTTCCAC
TGTGGGAACGGCTTGGCCCAGCGGGGCCTTTCCTCCACCACCTGCAGCTTCTCAGCCAATGCTGATTCT
GGGAACCAATGGCCCTTCCAAGAGCTGGTTTTAGAGCCCTCTCTGGGGATGAAGAACCCTCCCCAGCTG
GAAGATGATGCACTTGAAGGCTCAGCAAGCAACACACAAGGGCGTCAAGTCACTGGCCGGATTCGTGCC
TCCCTTGTCCTGATACTGAAGACCATCAGAAGAAGACTCCCGTTCAGCAAGTGGAGACTGGCATTCAGA
TTCGCTGGCCCGCATGCTGAGAGTGCAGAGATACCAAATACTGCTGAAAGGATGCAAAGGATGATAGGA
TGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001143987
Insert Size 2004 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001143987.1
RefSeq Size 2222 bp
RefSeq ORF 2004 bp
Locus ID 653149
UniProt ID Q5VWK0
MW 75.4 kDa
Gene Summary This gene is a member of the neuroblastoma breakpoint family (NBPF) which consists of dozens of recently duplicated genes primarily located in segmental duplications on human chromosome 1. This gene family has experienced its greatest expansion within the human lineage and has expanded, to a lesser extent, among primates in general. Members of this gene family are characterized by tandemly repeated copies of DUF1220 protein domains. Gene copy number variations in the human chromosomal region 1q21.1, where most DUF1220 domains are located, have been implicated in a number of developmental and neurogenetic diseases such as microcephaly, macrocephaly, autism, schizophrenia, cognitive disability, congenital heart disease, neuroblastoma, and congenital kidney and urinary tract anomalies. Altered expression of some gene family members is associated with several types of cancer. This gene family contains numerous pseudogenes. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2013]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). There are no full-length transcripts representing this variant that are specifically derived from this NBPF6 gene; it is supported by partial transcripts specifically derived from this location, by transcript alignments from the closely related NBPF4 family member (GeneID:148545), and by exon structure described in PMID:16079250. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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