FCHO2 (NM_001146032) Human Untagged Clone
CAT＃: SC327213
FCHO2 (untagged)-Human FCH domain only 2 (FCHO2) transcript variant 2
CNY 15010.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001146032, the custom clone sequence may differ by one or more nucleotides
ATGGTCATGGCGTATTTCGTCGAGAATTTTTGGGGGGAAAAAAATAGTGGCTTTGATGTC CTCTACCATAATATGAAACATGGACAGATATCAACAAAAGAACTAGCAGATTTTGTAAGG GAACGAGCTACCATAGAGGAGGCATACTCCAGGTCAATGACAAAACTAGCAAAATCTGCA AGCAATTATTCACAACTTGGAACATTTGCACCAGTATGGGATGTATTCAAAACATCTACA GAGAAATTAGCAAATTGTCACTTGGATCTTGTTAGAAAATTACAAGAATTAATAAAGGAA GTTCAGAAGTATGGAGAAGAACAAGTAAAGTCTCATAAAAAGACTAAAGAAGAAGTTGCA GGAACTCTGGAAGCTGTCCAAACCATTCAGAGCATAACTCAGGCCCTCCAGAAATCCAAG GAAAATTACAATGCCAAGTGTGTAGAACAGGAGCGTTTGAAAAAGGAAGGAGCTACACAA AGAGAAATAGAAAAGGCAGCTGTTAAATCTAAGAAAGCTACAGATACCTATAAACTCTAT GTGGAAAAATATGCATTAGCAAAAGCTGATTTCGAACAGAAAATGACAGAAACAGCTCAG GTCCATGAAGAATTTATAAATAACATGGCTAATACTACAGTTGAAAGTTTGATACAAAAA TTTGCTGAGTCAAAAGGCACTGGGAAGGAAAGACCTGGCCTCATTGAATTTGAAGAATGT GACACTGCTAGTGCAGTTGAAGGTATAAAACCAAGGAAAAGAAAGACCTTTGCTTTGCCA GGAATCATTAAAAAGGAAAAAGATGCAGAATCTGTGGAATGTCCTGATGCAGATTCATTG AACATTCCTGATGTAGATGAAGAAGGCTACAGTATTAAACCAGAAACAAATCAGAATGAT ACCAAAGAGAACCATTTCTACTCATCTAGTGATTCTGACTCCGAAGATGAAGAACCAAAG AAGTATCGGATAGAAATTAAGCCTATGCATCCAAATAACTCACATCACACAATGGCTTCT TTGGATGAATTAAAAGTATCTATAGGGAATATAACACTCTCCCCAGCAATATCTAGACAC AGTCCAGTACAGATGAATCGGAATTTGTCTAATGAGGAGTTAACAAAATCAAAGCCATCT GCTCCACCCAATGAAAAAGGAACCAGTGATTTACTTGCTTGGGACCCCCTATTTGGACCA TCTCTTGATTCATCTTCTTCATCTTCACTAACTTCATCATCATCAGCCAGGCCCACAACT CCTCTTTCTGTAGGCACCATTGTCCCACCTCCGAGGCCTGCTTCCAGACCAAAGCTTACT TCAGGCAAACTCAGTGGGATTAATGAAATACCCAGGCCATTCAGCCCACCTGTAACTTCC AACACCAGCCCACCTCCTGCTGCACCATTAGCCCGGGCAGAAAGTTCTTCTTCTATCTCA TCATCTGCTTCATTGAGTGCTGCCAATACTCCAACAGTAGGTGTGTCACGGGGTCCCAGC CCTGTCAGCCTTGGAAATCAGGATACCTTACCTGTGGCAGTTGCCCTTACAGAATCTGTT AATGCCTACTTTAAAGGAGCAGATCCCACCAAGTGTATTGTGAAGATCACTGGTGATATG ACAATGTCATTTCCAAGTGGAATTATTAAAGTCTTCACCAGCAATCCAACTCCAGCTGTG TTGTGCTTCAGGGTGAAAAATATCAGCAGACTAGAGCAGATTCTTCCAAATGCACAGCTT GTGTTCAGTGATCCATCACAATGTGATTCCAACACAAAAGATTTTTGGATGAACATGCAA GCTGTTACAGTCTACCTCAAGAAGCTGTCAGAGCAAAATCCAGCTGCTTCTTATTATAAT GTAGATGTATTAAAGTATCAGGTATCATCAAATGGTATTCAGTCCACTCCTCTGAATCTT GCAACATACTGGAAATGTAGTGCTAGCACCACAGATCTTAGAGTGGATTATAAATACAAT CCAGAAGCTATGGTGGCACCTAGTGTGCTTTCCAACATACAGGTGGTGGTACCAGTGGAT GGAGGAGTAACGAACATGCAGTCCCTTCCCCCTGCAATATGGAATGCAGAACAAATGAAA GCCTTTTGGAAATTGTCTAGTATTTCAGAAAAATCAGAAAATGGAGGTTCTGGGTCCCTC CGAGCAAAATTTGATCTTTCAGAAGGACCTAGTAAACCCACGACACTTGCAGTACAATTC CTCAGCGAGGGAAGTACCCTTTCAGGAGTAGATTTCGAACTTGTGGGCACTGGCTATAGG CTTTCCTTAATAAAGAAGCGGTTTGCTACTGGACGATACCTGGCGGATTGT |
| Restriction Sites | Please inquire |
| ACCN | NM_001146032 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001146032.1, NP_001139504.1 |
| RefSeq Size | 4895 bp |
| RefSeq ORF | 2334 bp |
| Locus ID | 115548 |
| UniProt ID | Q0JRZ9 |
| Gene Summary | Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. Has a lipid-binding activity with a preference for membranes enriched in phosphatidylserine and phosphoinositides (Pi(4,5) biphosphate) like the plasma membrane. Its membrane-bending activity might be important for the subsequent action of clathrin and adaptors in the formation of clathrin-coated vesicles. Involved in adaptor protein complex AP-2-dependent endocytosis of the transferrin receptor, it also functions in the AP-2-independent endocytosis of the LDL receptor.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon compared to variant 1. The encoded isoform (b) is shorter compared to isoform a. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228578 | FCHO2 (Myc-DDK-tagged)-Human FCH domain only 2 (FCHO2), transcript variant 2 |
CNY 5800.00 |
|
| RC228578L3 | Lenti-ORF clone of FCHO2 (Myc-DDK-tagged)-Human FCH domain only 2 (FCHO2), transcript variant 2 |
CNY 7700.00 |
|
| RC228578L4 | Lenti-ORF clone of FCHO2 (mGFP-tagged)-Human FCH domain only 2 (FCHO2), transcript variant 2 |
CNY 7700.00 |
|
| RG228578 | FCHO2 (tGFP-tagged) - Human FCH domain only 2 (FCHO2), transcript variant 2 |
CNY 6370.00 |
