DDHD1 (NM_001160147) Human Untagged Clone
CAT＃: SC327240
DDHD1 (untagged)-Human DDHD domain containing 1 (DDHD1) transcript variant 2
CNY 16910.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | iPLA1alpha; PA-PLA1; PAPLA1; SPG28 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001160147, the custom clone sequence may differ by one or more nucleotides
ATGAATTACCCGGGCCGCGGGTCCCCACGGAGCCCCGAGCATAACGGCCGAGGCGGCGGC GGCGGCGCCTGGGAGCTGGGCTCAGACGCGAGGCCAGCGTTCGGCGGCGGCGTCTGCTGC TTCGAGCACCTGCCCGGCGGGGACCCGGACGACGGCGACGTGCCCCTGGCCCTGCTGCGC GGGGAACCCGGGCTGCATTTGGCGCCGGGCACCGACGACCACAACCACCACCTCGCGCTG GACCCCTGCCTCAGTGACGAGAACTATGACTTCAGCTCCGCCGAGTCGGGCTCCTCGCTG CGCTACTACAGCGAGGGTGAGAGCGGCGGCGGCGGCAGCTCCTTGTCGCTGCACCCGCCG CAGCAGCCTCCGCTGGTCCCGACGAACTCGGGGGGCGGCGGCGCGACAGGAGGGTCCCCC GGGGAAAGGAAACGTACCCGGCTTGGCGGCCCGGCGGCCCGGCACCGCTATGAGGTAGTG ACGGAGCTGGGCCCGGAGGAGGTACGCTGGTTCTACAAGGAGGACAAGAAGACCTGGAAG CCCTTCATCGGCTACGACTCGCTCCGCATCGAGCTCGCCTTCCGGACCCTGCTGCAGACC ACGGGTGCCCGGCCCCAGGGCGGGGACCGGGACGGCGACCATGTGTGCTCCCCCACGGGC CCAGCCTCCAGTTCCGGAGAAGATGACGATGAGGACCGCGCCTGCGGCTTCTGCCAGAGT ACGACGGGGCACGAGCCGGAGATGGTGGAGCTTGTGAACATCGAGCCTGTGTGCGTGCGG GGCGGCCTCTACGAGGTGGATGTGACCCAAGGAGAGTGCTACCCGGTGTACTGGAACCAG GCTGATAAAATACCAGTAATGCGTGGACAGTGGTTTATTGACGGCACTTGGCAGCCTCTA GAAGAGGAAGAAAGTAATTTAATTGAGCAAGAACATCTCAATTGTTTTAGGGGCCAGCAG ATGCAGGAAAATTTCGATATTGAAGTGTCAAAATCCATAGATGGAAAAGATGGCAGTGGG ATCAACTATTCTGCTGTTCATAGTTTCAAGTTGAGTCGAAACCATGTGGACTGGCACAGT GTGGATGAAGTATATCTTTATAGTGATGCAACAACATCTAAAATTGCAAGAACAGTTACC CAAAAACTGGGATTTTCTAAAGCATCAAGTAGTGGTACCAGACTTCATAGAGGTTATGTA GAAGAAGCCACATTAGAAGACAAGCCATCACAGACTACCCATATTGTATTTGTTGTGCAT GGCATTGGGCAGAAAATGGACCAAGGAAGAATTATCAAAAATACAGCTATGATGAGAGAA GCTGCAAGAAAAATAGAAGAAAGGCATTTTTCCAACCATGCAACACATGTTGAATTTCTG CCTGTTGAGTGGCGGTCAAAACTTACTCTTGATGGAGACACTGTTGATTCCATTACTCCT GACAAAGTACGAGGTTTAAGGGATATGCTGAACAGCAGTGCAATGGACATAATGTATTAT ACTAGTCCACTTTATAGAGATGAACTAGTTAAAGGCCTTCAGCAAGAGCTGAATCGATTG TATTCCCTTTTCTGTTCTCGGAATCCAGACTTTGAAGAAAAAGGGGGTAAAGTCTCAATA GTATCACATTCCTTGGGATGTGTAATTACTTATGACATAATGACTGGCTGGAATCCAGTT CGGCTGTATGAACAGTTGCTGCAAAAGGAAGAAGAGTTGCCTGATGAACGATGGATGAGC TATGAAGAACGACATCTTCTTGATGAACTCTATATAACTAAACGACGGCTGAAGGAAATA GAAGAACGGCTTCACGGATTGAAAGCATCATCTATGACACAAACACCTGCCTTAAAATTT AAGGTTGAGAATTTCTTCTGTATGGGATCCCCATTAGCAGTTTTCTTGGCGTTGCGTGGC ATCCGCCCAGGAAATACTGGAAGTCAAGACCATATTTTGCCTAGAGAGATTTGTAACCGG TTACTAAATATTTTTCATCCTACAGATCCAGTGGCTTATAGATTAGAACCATTAATACTG AAACACTACAGCAACATTTCACCTGTCCAGATCCACTGGTACAATACTTCAAATCCTTTA CCTTATGAACATATGAAGCCAAGCTTTCTCAACCCAGCTAAAGAACCTACCTCAGTTTCA GAGAATGAAGGCATTTCAACCATACCAAGCCCTGTGACCTCACCAGTTTTGTCCCGCCGA CACTATGGAGAATCTATAACAAATATAGGCAAAGCAAGCATATTAGGGGCTGCTAGCATT GGAAAGGGACTTGGAGGAATGTTGTTCTCAAGATTTGGACGTTCATCTACAACACAGTCA TCTGAAACATCAAAAGACTCAATGGAAGATGAGAAGAAGCCAGTTGCCTCACCTTCTGCT ACCACCGTAGGGACACAGACCCTTCCACATAGCAGTTCTGGCTTCCTCGATTCTGCATTG GAGTTGGATCACAGGATTGATTTTGAACTCAGAGAAGGCCTTGTGGAGAGCCGCTATTGG TCAGCTGTCACGTCGCATACTGCCTATTGGTCATCCTTGGATGTTGCCCTTTTTCTTTTA ACCTTCATGTATAAACATGAGCACGATGATGATGCAAAACCCAATTTAGATCCAATC |
| Restriction Sites | Please inquire |
| ACCN | NM_001160147 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001160147.1, NP_001153619.1 |
| RefSeq Size | 12906 bp |
| RefSeq ORF | 2640 bp |
| Locus ID | 80821 |
| UniProt ID | Q8NEL9 |
| Gene Summary | This gene is a member of the intracellular phospholipase A1 gene family. The protein encoded by this gene preferentially hydrolyzes phosphatidic acid. It is a cytosolic protein with some mitochondrial localization, and is thought to be involved in the regulation of mitochondrial dynamics. Overexpression of this gene causes fragmentation of the tubular structures in mitochondria, while depletion of the gene results in mitochondrial tubule elongation. Deletion of this gene in male mice caused fertility defects, resulting from disruption in the organization of the mitochondria during spermiogenesis. In humans, mutations in this gene have been associated with hereditary spastic paraplegia (HSP), also known as Strumpell-Lorrain disease, or, familial spastic paraparesis (FSP). This inherited disorder is characterized by progressive weakness and spasticity of the legs. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015] Transcript Variant: This variant (2) includes an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (b) is longer than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228605 | DDHD1 (Myc-DDK-tagged)-Human DDHD domain containing 1 (DDHD1), transcript variant 2 |
CNY 9220.00 |
|
| RC228605L3 | Lenti-ORF clone of DDHD1 (Myc-DDK-tagged)-Human DDHD domain containing 1 (DDHD1), transcript variant 2 |
CNY 11120.00 |
|
| RC228605L4 | Lenti-ORF clone of DDHD1 (mGFP-tagged)-Human DDHD domain containing 1 (DDHD1), transcript variant 2 |
CNY 11120.00 |
|
| RG228605 | DDHD1 (tGFP-tagged) - Human DDHD domain containing 1 (DDHD1), transcript variant 2 |
CNY 10170.00 |
