FCHO1 (NM_001161359) Human Untagged Clone
CAT＃: SC327662
FCHO1 (untagged)-Human FCH domain only 1 (FCHO1) transcript variant 4
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001161359, the custom clone sequence may differ by one or more nucleotides
ATGGCGAAACTCTCCAAGCTGGCCAGCAACGGGACCCCCATGGGGACCTTCGCCCCGCTC TGGGAGGTCTTCCGCGTCTCCTCGGACAAGCTGGCGCTGTGCCACCTGGAACTGACACGG AAGTTACAGGATCTCATCAAGGACGTTCTCCGCTACGGCGAGGAACAGCTCAAGACCCAC AAGAAGTGCAAGGAGGAAGTGGTGAGCACCTTGGATGCTGTGCAGGTACTCTCGGGCGTC AGCCAGCTCCTGCCCAAGTCCCGCGAGAACTACCTGAACCGTTGCATGGACCAGGAGCGG CTGCGGAGGGAGAGTACCAGCCAGAAGGAGATGGACAAGGCGGAGACTAAAACCAAGAAG GCGGCAGAGAGCCTGCGGCGCTCAGTGGAAAAATACAACTCAGCCCGAGCTGACTTTGAG CAGAAGATGCTGGACTCAGCCCTGCGCTTCCAAGCCATGGAGGAGACACACCTGAGGCAC ATGAAGGCACTGCTGGGCTCATATGCTCACTCGGTGGAGGACACGCACGTGCAGATTGGG CAGGTGCATGAGGAATTTAAGCAGAACATCGAGAACGTCAGCGTGGAGATGCTACTCAGG AAGTTTGCAGAGAGTAAGGGCACAGGCCGGGAGAAGCCTGGACCTCTGGACTTCGAGGCA TACAGTGCGGCTGCCCTGCAGGAAGCGATGAAACGTTTGCGGGGAGCCAAGGCCTTTCGC CTTCCAGGACTAAGCCGGCGGGAGCGGGAGCCAGAGCCACCTGCAGCTGTAGATTTCCTG GAGCCCGATTCAGGGACATGTCCAGAGGTGGATGAAGAAGGTTTCACTGTCCGGCCTGAT GTGACCCAGAACAGCACGGCCGAGCCCTCCCGTTTCTCGTCCAGCGACTCCGACTTCGAC GATGAAGAGCCCCGCAAGTTCTATGTGCACATCAAGCCTGCCCCGGCCCGGGCTCCAGCC TGCAGCCCCGAGGCAGCAGCGGCACAGCTCAGGGCCACCGCGGGCAGCCTCATCCTTCCT CCTGGCCCAGGGGGCACCATGAAACGCCATTCTTCACGGGACGCTGCTGGGAAACCCCAG AGACCTCGGTCTGCCCCCAGAACCAGCAGCTGTGCAGAGAGATTGCAGTCAGAGGAGCAG GTGTCCAAGAACCTCTTTGGGCCGCCCCTGGAGTCAGCCTTTGACCACGAAGATTTTACA GGCTCTAGCAGCCTGGGCTTCACCTCCAGCCCCTCCCCTTTCTCCTCCTCGTCGCCCGAA AACGTGGAGGATTCCGGCCTGGACTCTCCGTCCCACGCGGCACCTGGCCCCTCCCCAGAT TCCTGGGTCCCCCGCCCAGGCACCCCGCAGAGCCCGCCCAGCTGTAGGGCGCCACCCCCA GAGGCCAGGGGTATCCGGGCACCGCCTCTGCCAGACTCGCCGCAGCCCCTCGCCTCGTCT CCAGGCCCCTGGGGGCTGGAGGCCTTGGCCGGAGGAGACCTGATGCCTGCACCTGCTGAC CCCACAGCCAGGGAGGGCCTGGCAGCCCCACCCAGGAGACTTCGCTCTAGGAAGGTGTCC TGCCCTCTCACACGTAGCAATGGGGACCTGTCTCGTTCCCTGAGCCCCTCCCCACTGGGC TCTTCAGCCGCCAGCACTGCCTTGGAACGGCCCAGCTTCTTATCCCAGACAGGACACGGA GTCTCCCGGGGTCCGAGCCCTGTGGTCCTGGGCTCCCAGGATGCCCTGCCCATAGCCACA GCCTTCACAGAGTATGTCCACGCCTACTTCCGTGGCCACAGCCCCAGCTGCCTGGCTCGA GTAACTGGGGAGCTGACCATGACCTTCCCTGCTGGCATCGTGCGTGTGTTCAGCGGGACC CCACCACCACCTGTCCTCAGCTTCCGGCTTGTACACACAACCGCTATTGAGCACTTCCAG CCCAACGCCGATCTGCTGTTCAGTGACCCCTCCCAGAGTGACCCTGAGACCAAAGACTTC TGGCTCAACATGGCAGCTCTGACCGAAGCCCTGCAGCGCCAGGCAGAGCAGAACCCCACT GCCTCCTACTACAACGTGGTGCTGCTGCGATACCAGTTCTCCCGCCCGGGTCCCCAGTCT GTGCCTCTGCAGCTCAGTGCCCACTGGCAGTGTGGAGCCACCCTCACCCAGGTCTCAGTG GAGTACGGCTACCGGCCCGGTGCCACGGCTGTGCCCACACCACTCACGAACGTCCAGATC CTGCTGCCTGTGGGGGAGCCTGTGACCAACGTCCGCTTGCAGCCGGCTGCCACCTGGAAC CTGGAGGAGAAGCGGCTCACTTGGAGGCTTCCAGATGTGTCCGAGGCAGGCGGTTCTGGC CGCCTCTCTGCCAGCTGGGAGCCGCTCTCAGGGCCCAGCACACCCAGCCCCGTGGCTGCA CAGTTCACCAGCGAGGGGACCACTCTGTCGGGCGTGGACTTGGAACTGGTGGGCAGCGGT TACCGCATGTCGCTGGTGAAGAGGAGGTTTGCCACAGGGATGTACCTGGTGAGCTGC |
| Restriction Sites | Please inquire |
| ACCN | NM_001161359 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001161359.1, NP_001154831.1 |
| RefSeq Size | 3061 bp |
| RefSeq ORF | 2520 bp |
| Locus ID | 23149 |
| UniProt ID | O14526 |
| Gene Summary | Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. May regulate Bmp signaling by regulating clathrin-mediated endocytosis of Bmp receptors.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. This variant (4) also uses an alternate splice site in the 3' coding region compared to variant 1, that results in a frameshift. The resulting isoform (c) is shorter and has a distinct C-terminus compared to isoform a. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229027 | FCHO1 (Myc-DDK-tagged)-Human FCH domain only 1 (FCHO1), transcript variant 4 |
CNY 7130.00 |
|
| RC229027L3 | Lenti-ORF clone of FCHO1 (Myc-DDK-tagged)-Human FCH domain only 1 (FCHO1), transcript variant 4 |
CNY 9030.00 |
|
| RC229027L4 | Lenti-ORF clone of FCHO1 (mGFP-tagged)-Human FCH domain only 1 (FCHO1), transcript variant 4 |
CNY 9030.00 |
|
| RG229027 | FCHO1 (tGFP-tagged) - Human FCH domain only 1 (FCHO1), transcript variant 4 |
CNY 7890.00 |
