NBPF15 (NM_001170755) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AB14; AG3; NBPF16
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001170755, the custom clone sequence may differ by one or more nucleotides ATGGTGGTATCAGCCGGCCCTTTGTCCAGCGAGAAGGCAGAGATGAACATTCTAGAAATC AATGAGAAATTGCGCCCCCAGTTGGCAGAGAAGAAACAGCAGTTCAGAAACCTCAAAGAG AAATGTTTTCTAACTCAACTGGCCGGCTTCCTGGCCAACCGACAGAAGAAATACAAATAT GAAGAGTGCAAAGATCTCATAAAATTTATGCTGAGGAATGAGCGACAGTTCAAGGAGGAG AAGCTTGCAGAGCAGCTCAAGCAAGCTGAGGAGCTCAGGCAATATAAAGTCCTGGTTCAC GCTCAGGAACGAGAGCTGACCCAGTTAAGGGAGAAGTTACGGGAAGGGAGAGATGCCTCC CGCTCATTGAATGAGCATCTCCAGGCCCTCCTCACTCCGGATGAGCCGGACAAGTCCCAG GGGCAGGACCTCCAAGAACAGCTGGCTGAGGGGTGTAGACTGACACAGCACCTTGTCCAA AAGCTCAGCCCAGAAAATGACAACGATGACGATGAAGATGTTCAAGTTGAGGTGGCTGAG AAAGTGCAGAAATCGTCTGCCCCCAGGGAGATGCAGAAGGCTGAAGAAAAGGAAGTCCCT GAGGACTCACTGGAGGAATGTGCCATCACTTGTTCAAATAGCCATGGCCCTTATGACTCC AACCAGCCACATAAGAAAACCAAAATCACATTTGAGGAAGACAAAGTCGACTCAACTCTC ATTGGCTCATCCTCTCATGTTGAATGGGAGGATGCTGTACACATTATTCCAGAAAATGAA AGTGATGATGAGGAAGAGGAAGAAAAAGGGCCAGTGTCTCCCAGGAATCTGCAGGAGTCT GAAGAGGAGGAAGTCCCCCAGGAGTCCTGGGATGAAGGTTATTCGACTCTCTCAATTCCT CCTGAAATGTTGGCCTCGTACCAGTCTTACAGCAGCACATTTCACTCATTAGAGGAACAG CAAGTCTGCATGGCTGTTGACATAGGCAGACATCGGTGGGATCAAGTGAAAAAGGAGGAC CAAGAGGCAACAGGTCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTC TTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGAC TCATGCCAGCCCTACAGAAGTGCCTTTTACGTATTGGAGCAACAGCGTGTTGGCTTGGCT ATTGACATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGC CCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTG GATAGATGTTATTCAACTCCTTCAGATTATCTTGAACTGCCTGACTTAGGCCAGCCCTAC AGCAGTGCTGTTTACTCATTGGAGGAACAGTACCTTGGCTTGGCTCTTGACGTGGACAGA ATTAAAAAGGACCAAGAAGAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGG GAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCA ACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT GCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGG AAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGAAAAGAAGAAGGGGAAGAAAAGAA GGGGAAGATGACAACCCACCATGCCCCAGGCTCTACGGCGTGCTGATGGAAGTGGAAGAG CCTGAAGTCTTACAGGACTCACTGGATAGATGTTATTCGACTCCGTCAATGTACTTTGAA CAACCTGACTCATTCCAGCACTACAGAAGTGTGTTTTACTCATTTGAGGAAGAGCATATC AGCTTCGCCCTTTACGTGGACAATAGGTTTTTTACTTTGACGGTGACAAGTCTCCACCTG GTGTTCCAGATGGGAGTCATATTCCCACAATAA
Restriction Sites	Please inquire
ACCN	NM_001170755
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001170755.1, NP_001164226.1
RefSeq Size	4802 bp
RefSeq ORF	2013 bp
Locus ID	284565
UniProt ID	Q8N660
Gene Summary	This gene is a member of the neuroblastoma breakpoint family (NBPF) which consists of dozens of recently duplicated genes primarily located in segmental duplications on human chromosome 1. This gene family has experienced its greatest expansion within the human lineage and has expanded, to a lesser extent, among primates in general. Members of this gene family are characterized by tandemly repeated copies of DUF1220 protein domains. Gene copy number variations in the human chromosomal region 1q21.1, where most DUF1220 domains are located, have been implicated in a number of developmental and neurogenetic diseases such as microcephaly, macrocephaly, autism, schizophrenia, cognitive disability, congenital heart disease, neuroblastoma, and congenital kidney and urinary tract anomalies. Altered expression of some gene family members is associated with several types of cancer. This gene family contains numerous pseudogenes. [provided by RefSeq, May 2013] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. This variant may include additional exon structure in the 5' UTR, but alternative splicing choices are present further upstream, and there are no 5' complete transcripts representing this variant.

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