Mucolipin 3 (MCOLN3) (NM_001253693) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	TRP-ML3; TRPML3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330301 representing NM_001253693. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCAGATCCTGAGGTAGTTGTGAGTAGCTGCAGCTCTCATGAAGAGGAAAATCGCTGCAATTTTAAC CAGCAAACATCTCCATCTGAGGAGCTTCTATTAGAAGACCAGATGAGGCGAAAACTCAAATTTTTTTTC ATGAATCCCTGTGAGAAGTTCTGGGCTCGAGGTAGAAAACCATGGAAACTTGCCATACAAATTCTAAAA ATTGCAATGGTGACTATCCAGTACTTGCAGCTATACAATGTCTCCGTTGGGAATCATGCTTATGAGAAC AAAGGTACCAAGCAATCTGCTATGGCAATCTGTCAGCACTTCTACAAGCGAGGAAACATCTACCCTGGA AATGATACCTTTGACATCGATCCAGAAATTGAAACTGAGTGTTTCTTTGTGGAGCCAGATGAACCTTTT CACATTGGGACACCAGCAGAAAATAAACTGAACTTAACACTGGACTTCCACAGACTCCTAACAGTGGAG CTTCAGTTTAAACTGAAAGCCATTAATCTGCAGACAGTTCGTCATCAAGAACTCCCTGACTGTTATGAC TTTACTCTGACTATAACATTTGACAACAAGGCCCATAGTGGAAGAATTAAAATAAGTTTAGATAATGAC ATTTCCATCAGAGAATGTAAAGACTGGCATGTATCTGGATCAATTCAGAAGAACACTCATTACATGATG ATCTTTGATGCCTTTGTCATTCTGACTTGCTTGGTTTCATTAATCCTCTGCATTAGATCTGTGATTAGA GGACTTCAGCTTCAGCAGGAGTTTGTCAATTTTTTCCTCCTCCATTATAAGAAGGAAGTTTCTGTTTCT GATCAAATGGAATTTGTCAATGGATGGTACATTATGATTATTATTAGTGACATATTGACAATCATTGGA TCAATTCTAAAAATGGAAATCCAAGCTAAGAGTCTAACTAGTTATGATGTCTGTAGCATACTTCTTGGG ACTTCTACCATGCTCGTGTGGCTTGGAGTCATCCGATACCTCGGTTTCTTTGCAAAGTACAACCTCCTC ATTTTGACCCTTCAGGCAGCGCTGCCCAATGTCATCAGGTTCTGCTGCTGTGCAGCTATGATTTACTTA GGTTACTGCTTCTGTGGATGGATCGTGCTGGGGCCTTACCATGACAAGTTTCGTTCTCTGAACATGGTC TCTGAGTGCCTTTTCTCTCTGATAAATGGAGATGATATGTTTGCCACGTTTGCAAAAATGCAGCAAAAA AGTTACTTAGTCTGGCTGTTTAGTAGAATTTACCTCTACTCATTCATCAGCCTCTTTATATATATGATT TTAAGTCTTTTCATTGCACTGATCACTGATACATACGAAACAATTAAGCAATACCAACAAGATGGCTTC CCAGAGACTGAACTTCGTACATTTATATCAGAATGCAAAGATCTACCCAACTCTGGAAAATACAGATTA GAAGATGACCCTCCAGTATCTTTATTCTGCTGTTGTAAAAAGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001253693
Insert Size	1494 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001253693.1
RefSeq Size	2699 bp
RefSeq ORF	1494 bp
Locus ID	55283
UniProt ID	Q8TDD5
Protein Families	Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
MW	57.7 kDa
Gene Summary	This gene encodes one of members of the mucolipin cation channel proteins. Mutation studies of the highly similar protein in mice have shown that the protein is found in cochlea hair cells, and mutant mice show early-onset hearing loss and balance problems. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1.

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