ENC1 (NM_001256576) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CCL28; ENC-1; KLHL35; KLHL37; NRPB; PIG10; TP53I10
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330459 representing NM_001256576. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTTCAGTGGTGGCCTGAAAGAGAGCCAGGACAGTGAGGTCAACTTTGACAATTCCATCCACCCAGAA GTCTTGGAGCTGCTGCTTGACTATGCGTACTCCTCCCGGGTCATCATCAATGAAGAAAATGCAGAATCG CTCCTGGAAGCTGGTGACATGCTGGAGTTTCAAGACATCCGGGATGCATGTGCAGAGTTCCTGGAAAAG AACCTGCATCCCACCAACTGCCTGGGCATGCTGCTGCTGTCTGATGCACACCAGTGCACCAAGCTGTAC GAACTATCTTGGAGAATGTGTCTCAGCAACTTCCAAACCATCAGGAAGAATGAAGATTTCCTCCAGCTG CCCCAGGACATGGTAGTGCAACTCTTGTCCAGTGAAGAGCTGGAGACAGAGGATGAAAGGCTTGTGTAC GAGTCTGCAATTAACTGGATCAGCTATGACCTGAAGAAGCGCTATTGCTACCTCCCAGAACTGTTGCAG ACAGTAAGGCTGGCACTTCTGCCAGCCATCTATCTCATGGAGAATGTGGCCATGGAGGAACTCATCACC AAGCAGAGAAAGAGTAAGGAAATTGTGGAAGAGGCCATCAGGTGCAAACTGAAAATCCTGCAGAATGAC GGTGTGGTAACCAGCCTCTGTGCCCGACCTCGGAAAACTGGCCATGCCCTCTTCCTTCTGGGAGGACAG ACTTTCATGTGTGACAAGTTGTATCTGGTAGACCAGAAGGCCAAAGAAATCATTCCCAAGGCTGACATT CCCAGCCCAAGAAAAGAGTTTAGTGCATGTGCGATTGGCTGCAAAGTGTACATTACTGGGGGGCGGGGG TCTGAAAATGGGGTCTCAAAAGATGTCTGGGTTTATGATACCCTGCACGAGGAGTGGTCCAAGGCTGCC CCCATGCTGGTGGCCAGGTTTGGCCATGGCTCTGCTGAACTGAAGCACTGCCTGTATGTGGTTGGGGGG CACACGGCCGCAACTGGCTGCCTCCCGGCCTCCCCCTCAGTCTCTCTAAAGCAGGTAGAACATTATGAC CCCACAATCAACAAATGGACCATGGTGGCCCCACTCCGAGAAGGCGTTAGCAACGCCGCAGTAGTGAGT GCCAAACTTAAGTTATTTGCTTTCGGAGGTACCAGTGTCAGTCATGACAAGCTCCCCAAAGTTCAGTGT TACGATCAGTGTGAAAACAGGTGGACTGTACCGGCCACCTGTCCCCAGCCCTGGCGTTACACAGCAGCA GCTGTGCTGGGGAACCAGATTTTTATTATGGGGGGTGATACAGAATTCTCTGCCTGCTCTGCTTATAAA TTCAACAGTGAGACTTACCAGTGGACCAAGGTGGGAGATGTGACAGCAAAGCGCATGAGCTGCCATGCT GTGGCCTCTGGAAACAAACTCTACGTGGTTGGAGGATACTTTGGCATTCAGCGATGCAAGACTTTGGAC TGCTACGATCCAACATTAGACGTGTGGAACAGCATCACCACTGTCCCGTACTCGCTGATTCCTACTGCA TTTGTCAGCACCTGGAAACATCTGCCTTCTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256576
Insert Size	1551 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256576.1
RefSeq Size	5384 bp
RefSeq ORF	1551 bp
Locus ID	8507
UniProt ID	O14682
Protein Families	Druggable Genome
MW	57.9 kDa
Gene Summary	This gene encodes a member of the kelch-related family of actin-binding proteins. The encoded protein plays a role in the oxidative stress response as a regulator of the transcription factor Nrf2, and expression of this gene may play a role in malignant transformation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012] Transcript Variant: This variant (4) uses an alternate splice site, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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