QTRTD1 (QTRT2) (NM_001256836) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	QTRTD1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330530 representing NM_001256836. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTTTGTTGGCAGTCTGTGTCTGTGTGGAGTGTTGCAGGACGAGTGGAAATGACTGTTTCCAAGTTC ATGGCAATTCAGAAGGCCCTTCAGCCAGACTGGTTCCAGTGCCTCTCCGATGGAGAAGTATCTTGTAAG GAAGCAACTTCCATAAAAAGGGTCAGAAAGTCTGTTGACCGATCACTTCTTTTCTTGGATAACTGTCTG CGGCTGCAGGAAGAGTCAGAGGTTCTTCAGAAGAGTGTGATCATTGGAGTGATTGAAGGTGGAGATGTG ATGGAAGAGAGGCTGAGGTCAGCACGAGAGACAGCCAAGCGGCCTGTGGGTGGCTTCCTTCTGGATGGT TTTCAAGGAAATCCAACAACCCTGGAGGCTAGACTACGCTTGCTGTCATCAGTCACTGCAGAGCTGCCG GAGGACAAGCCAAGGCTCATATCTGGTGTTAGTCGGCCAGATGAGGTGCTCGAGTGTATTGAAAGAGGA GTGGACTTATTTGAGAGTTTTTTCCCTTATCAAGTAACAGAGCGGGGATGTGCCCTGACTTTCAGTTTT GATTACCAGCCGAATCCTGAAGAGACACTACTACAACAAAATGGAACACAAGAAGAAATAAAATGTATG GATCAAATAAAGAAAATTGAAACAACTGGTTGCAACCAAGAAATAACATCATTTGAAATTAATCTGAAG GAAAAAAAGTACCAGGAGGACTTTAACCCGCTGGTGAGAGGATGTTCCTGTTACTGCTGTAAGAATCAC ACTCGGGCATACATCCACCATCTGCTGGTGACCAATGAGCTGCTGGCCGGAGTCCTGCTTATGATGCAC AACTTTGAACACTACTTTGGGTTTTTCCATTACATCCGGGAAGCACTAAAAAGTGACAAACTGGCACAG TTGAAAGAGCTCATCCACAGGCAAGCATCTTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001256836
Insert Size	930 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256836.1
RefSeq Size	3563 bp
RefSeq ORF	930 bp
Locus ID	79691
UniProt ID	Q9H974
MW	35.4 kDa
Gene Summary	This gene encodes a subunit of tRNA-guanine transglycosylase. tRNA-guanine transglycosylase is a heterodimeric enzyme complex that plays a critical role in tRNA modification by synthesizing the 7-deazaguanosine queuosine, which is found in tRNAs that code for asparagine, aspartic acid, histidine, and tyrosine. The encoded protein may play a role in the queuosine 5'-monophosphate salvage pathway. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012] Transcript Variant: This variant (3) lacks four consecutive internal exons and initiates translation at an upstream start codon, compared to variant 1. The encoded isoform (3) is shorter and has a distinct N-terminus, compared to isoform 1.

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