HERC3 (NM_001271602) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330944 representing NM_001271602. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGACTACTGAGGATTCTGTGGCAGTGCCCAGGTTAATACAAAAGCTGAACCAGCAAACAATATTACAA GTTTCCTGTGGCAACTGGCATTGCTTGGCTCTTGCGGCTGATGGCCAGTTCTTCACCTGGGGAAAGAAC AGCCATGGGCAGCTTGGCTTAGGGAAGGAGTTCCCCTCCCAAGCCAGCCCACAGAGGGTGAGGTCCCTG GAGGGGATCCCACTGGCTCAGGTGGCTGCCGGAGGGGCTCACAGCTTTGCCCTGTCTCTCTCAGGAGCT GTTTTTGGCTGGGGGATGAATAATGCCGGGCAGCTAGGGCTCAGTGATGAAAAAGATCGAGAATCTCCA TGCCATGTAAAACTCTTACGCACGCAAAAAGTTGTCTATATTAGTTGTGGAGAAGAACACACAGCAGTT CTCACAAAGAGTGGAGGTGTGTTTACCTTTGGCGCTGGTTCCTGTGGGCAACTTGGACACGACTCCATG AATGATGAGGTTAACCCTAGAAGAGTTCTAGAGCTGATGGGTAGTGAAGTAACTCAAATTGCTTGTGGC AGACAACATACCCTAGCCTTCGTGCCTTCTTCTGGACTCATCTATGCATTTGGTTGTGGAGCAAGAGGT CAATTAGGAACTGGGCACACTTGTAATGTTAAGTGCCCATCTCCTGTCAAGGGTTACTGGGCTGCCCAC AGTGGCCAGCTTTCAGCCCGAGCTGATCGCTTTAAATATCATATCGTTAAGCAGATCTTCTCTGGAGGA GACCAGACTTTTGTACTTTGCTCCAAATACGAGAATTATTCTCCTGCTGTTGACTTCAGGACTATGAAC CAAGCACATTATACCAGTTTAATAAATGATGAAACCATAGCAGTTTGGAGACAAAAACTCTCAGAACAC AACAATGCAAATACAATCAATGGTGTTGTTCAGATATTATCTTCTGCAGCCTGTTGGAATGGAAGTTTT CTTGAAAAAAAAATTGATGAACATTTTAAAACGAGTCCCAAAATCCCTGGGATTGACCTGAACTCAACT AGGGTGTTATTTGAGAAGTTAATGAACTCTCAGCACTCCATGATTCTAGAACAGATTTTGAACAGTTTT GAAAGTTGTCTGATTCCCCAGTTGTCAAGCTCACCACCAGATGTTGAAGCCATGAGAATCTATTTAATA CTACCTGAGTTTCCCCTACTCCAGGATTCCAAGTATTATATAACATTGACTATTCCCTTGGCTATGGCC ATTCTTCGGCTGGATACAAACCCCAGCAAAGTACTAGATAACTGGTGGTCTCAGGTATGCCCGAAATAT TTCATGAAGCTGGTAAACCTCTATAAAGGTGCAGTCCTTTATCTACTGAGGGGAAGAAAGACATTCTTA ATTCCCGTACTGTTTAACAATTATATCACAGCAGCTCTCAAACTCTTGGAGAAGTTATATAAGGTAAAT CTTAAAGTGAAGCATGTGGAATATGATACATTTTACATTCCTGAGATTTCCAATCTCGTGGACATTCAG GAAGACTACCTCATGTGGTTCTTGCATCAAGCAGGGATGAAGGCTAGACCATCAATAATACAGGATACT GTAACACTTTGTTCCTACCCTTTCATCTTTGATGCCCAAGCCAAGACCAAAATGTTACAGACAGATGCT GAACTACAGATGCAGGTGGCAGTCAATGGAGCCAACCTGCAGAATGTCTTCATGCTTCTCACCCTGGAG CCTCTGCTGGCCAGAAGCCCCTTCCTGGTCCTTCACGTTCGCAGGAACAACCTTGTTGGAGATGCCCTA AGAGAGCTGAGCATTCATTCTGATATTGATTTGAAAAAGCCTCTCAAAGTAATCTTTGATGGTGAAGAA GCAGTGGATGCCGGTGGTGTTACAAAGGAATTTTTTCTTTTGCTGTTAAAAGAACTTTTGAATCCCATC TATGGAATGTTTACCTACTATCAAGATTCAAATCTCTTGTGGTTTTCAGACACGTGTTTTGTAGAGCAC AACTGGTTTCACTTGATTGGTATAACCTGTGGACTAGCTATCTACAACTCCACTGTGGTCGATCTCCAC TTCCCATTGGCTCTCTACAAGAAGTTACTCAATGTAAAGCCTGGCTTGGAAGACTTAAAGGAGTTGTCA CCCACTGAAGGAAGGAGTCTCCAAGAGCTTTTAGATTACCCCGGGGAGGATGTGGAGGAGACTTTCTGC CTCAACTTCACGATCTGCCGAGAAAGCTATGGAGTGATTGAACAGAAGAAGCTGATACCTGGGGGAGAT AATGTAACTGTGTGCAAGGATAACAGGCAGGAATTTGTGGATGCTTATGTGAATTATGTCTTCCAAATC TCAGTTCATGAATGGTACACAGCCTTCTCTAGTGGCTTCCTAAAGGTGTGTGGTGGCAAAGTACTTGAG CTCTTCCAGCCTTCAGAACTGAGGGCTATGATGGTGGGGAACAGCAACTACAACTGGGAAGAACTGGAA GAGACTGCCATCTACAAGGGAGATTACTCGGCCACACATCCCACTGTAAAACTATTTTGGGAAACATTT CATGAGTTTCCATTGGAAAAGAAGAAGAAGTTTCTCTTGTTCCTGACAGGCAGCGATCGGATTCCCATC TACGGCATGGCCAGTCTGCAGATTGTCATCCAGTCCACAGCCAGCGGGGAGGAGTACTTGCCGGTGGCC CACACTTGCTACAACCTTCTTGACCTCCCCAAGTACAGCAGCAAAGAGATTCTGAGTGCCCGGCTGACC CAGGCCCTTGACAACTATGAAGGGTTTAGTTTGGCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001271602
Insert Size	2799 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271602.1
RefSeq Size	4730 bp
RefSeq ORF	2799 bp
Locus ID	8916
UniProt ID	Q15034
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis
MW	104.7 kDa
Gene Summary	This gene encodes a member the HERC ubiquitin ligase family. The encoded protein is located in the cytosol and binds ubiquitin via a HECT domain. Mutations in this gene have been associated with colorectal and gastric carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012] Transcript Variant: This variant (2) lacks an alternate 5' exon and portion of the 5' coding region. These differences cause translation initiation at a downstream AUG and result in an isoform (2) which has a shorter N-terminus, compared to isoform 1.

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