DUS2L (DUS2) (NM_001271763) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DUS2L; SMM1; URLC8
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330967 representing NM_001271763. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGATTTTGAATAGCCTCTCTCTGTGTTACCATAATAAGCTAATCCTGGCCCCAATGGTTCGGGTAGGG ACTCTTCCAATGAGGCTGCTGGCCCTGGATTATGGAGCGGACATTGTTTACTGTGAGGAGCTGATCGAC CTCAAGATGATTCAGTGCAAGAGAGTTGTTAATGAGGTGCTCAGCACAGTGGACTTTGTCGCCCCTGAT GATCGAGTTGTCTTCCGCACCTGTGAAAGAGAGCAGAACAGGGTGGTCTTCCAGATGGGAGGAATGGGA GCTGCCCTGCTGTCAGACCCTGACAAGATTGAGAAGATCCTCAGCACTCTTGTTAAAGGGACACGCAGA CCTGTGACCTGCAAGATTCGCATCCTGCCATCGCTAGAAGATACCCTGAGCCTTGTGAAGCGGATAGAG AGGACTGGCATTGCTGCCATCGCAGTTCATGGGAGGAAGCGGGAGGAGCGACCTCAGCATCCTGTCAGC TGTGAAGTCATCAAAGCCATTGCTGATACCCTCTCCATTCCTGTCATAGCCAACGGAGGATCTCATGAC CACATCCAACAGTATTCGGACATAGAGGACTTTCGACAAGCCACGGCAGCCTCTTCCGTGATGGTGGCC CGAGCAGCCATGTGGAACCCATCTATCTTCCTCAAGGAGGGTCTGCGGCCCCTGGAGGAGGTCATGCAG AAATACATCAGATACGCGGTGCAGTATGACAACCACTACACCAACACCAAGTACTGCTTGTGCCAGATG CTACGAGAACAGCTGGAGTCGCCCCAGGGAAGGTTGCTCCATGCTGCCCAGTCTTCCCGGGAAATTTGT GAGGCCTTTGGCCTTGGTGCCTTCTATGAGGAGACCACACAGGAGCTGGATGCCCAGCAGGCCAGGCTC TCAGCCAAGACTTCAGAGCAGACAGGGGAGCCAGCTGAAGATACCTCTGGTGTCATTAAGATGGCTGTC AAGTTTGACCGGAGAGCATACCCAGCCCAGATCACCCCTAAGATGTGCCTACTAGAGTGGTGCCGGAGG GAGAAGTTGGCACAGCCTGTGTATGAAACGGTTCAACGCCCTCTAGATCGCCTGTTCTCCTCTATTGTC ACCGTTGCTGAACAAAAGTATCAGTCTACCTTGTGGGACAAGTCCAAGAAACTGGCGGAGCAGGCTGCA GCCATCGTCTGTCTGCGGAGCCAGGGCCTCCCTGAGGGTCGGCTGGGTGAGGAGAGCCCTTCCTTGCAC AAGCGAAAGAGGGAGGCTCCTGACCAAGACCCTGGGGGCCCCAGAGCTCAGGAGCTAGCACAACCTGGG GATCTGTGCAAGAAGCCCTTTGTGGCCTTGGGAAGTGGTGAAGAAAGCCCCCTGGAAGGCTGGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001271763
Insert Size	1377 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271763.1
RefSeq Size	2237 bp
RefSeq ORF	1377 bp
Locus ID	54920
UniProt ID	Q9NX74
MW	51.4 kDa
Gene Summary	This gene encodes a cytoplasmic protein that catalyzes the conversion of uridine residues to dihydrouridine in the D-loop of tRNA. The resulting modified bases confer enhanced regional flexibility to tRNA. The encoded protein may increase the rate of translation by inhibiting an interferon-induced protein kinase. This gene has been implicated in pulmonary carcinogenesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012] Transcript Variant: This variant (3) lacks two alternate exons, resulting in the loss of an in-frame segment in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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