PUS3 (NM_001271985) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	2610020J05Rik; FKSG32; MRT55; NEDMIGS
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331017 representing NM_001271985. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGATTATGCAGCTCAGAAGTATGTTGGCACCCATGATTTCAGGAACTTGTGTAAAATGGATGTAGCC AACGGTGTGATTAATTTTCAGAGGACTATTCTATCTGCTCAAGTACAGCTAGTGGGCCAGAGCCCAGGT GAGGGGAGATGGCAAGAACCTTTCCAGTTATGTCAGTTTGAAGTGACTGGCCAGGCATTCCTTTATCAT CAAGTCCGATGTATGATGGCTATCCTCTTTCTGATTGGCCAAGGAATGGAGAAGCCAGAGATTATTGAT GAGCTGCTGAATATAGAGAAAAATCCCCAAAAGCCTCAATATAGTATGGCTGTAGAATTTCCTCTAGTC TTATATGACTGTAAGTTTGAAAATGTCAAGTGGATCTATGACCAGGAGGCTCAGGAGTTCAATATTACC CACCTACAACAACTGTGGGCTAATCATGCTGTCAAAACTCACATGTTGTATAGTATGCTACAAGGACTG GACACTGTTCCAGTACCCTGTGGAATAGGACCAAAGATGGATGGAATGACAGAATGGGGAAATGTTAAG CCCTCTGTCATAAAGCAGACCAGTGCCTTTGTAGAAGGAGTGAAGATGCGCACATATAAGCCCCTCATG GACCGTCCTAAATGCCAAGGACTGGAATCCCGGATCCAGCATTTTGTACGTAGGGGACGAATTGAGCAC CCACATTTATTCCATGAGGAAGAAACAAAAGCCAAAAGGGACTGTAATGACACACTAGAGGAAGAGAAT ACTAATTTGGAGACACCAACGAAGAGGGTCTGTGTTGACACAGAAATTAAAAGCATCATTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271985
Insert Size	822 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271985.1
RefSeq Size	1438 bp
RefSeq ORF	822 bp
Locus ID	83480
UniProt ID	Q9BZE2
MW	31.6 kDa
Gene Summary	The protein encoded by this gene catalyzes the formation of tRNA pseudouridine from tRNA uridine at position 39 in the anticodon stem and loop of transfer RNAs. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012] Transcript Variant: This variant (2) lacks an alternate coding exon and uses a downstream start codon compared to variant 1. It encodes isoform 2, which has a shorter N-terminus compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.