PUS3 (NM_001271985) Human Untagged Clone

CAT#: SC331017

PUS3 (untagged) - Homo sapiens pseudouridylate synthase 3 (PUS3), transcript variant 2



  "NM_001271985" in other vectors (2)

CNY 2950.00


货期*
4周

规格
    • 10 ug

Product images

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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms 2610020J05Rik; FKSG32; MRT55; NEDMIGS
Vector pCMV6-Entry
Sequence Data
>SC331017 representing NM_001271985.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

ATGGATTATGCAGCTCAGAAGTATGTTGGCACCCATGATTTCAGGAACTTGTGTAAAATGGATGTAGCC
AACGGTGTGATTAATTTTCAGAGGACTATTCTATCTGCTCAAGTACAGCTAGTGGGCCAGAGCCCAGGT
GAGGGGAGATGGCAAGAACCTTTCCAGTTATGTCAGTTTGAAGTGACTGGCCAGGCATTCCTTTATCAT
CAAGTCCGATGTATGATGGCTATCCTCTTTCTGATTGGCCAAGGAATGGAGAAGCCAGAGATTATTGAT
GAGCTGCTGAATATAGAGAAAAATCCCCAAAAGCCTCAATATAGTATGGCTGTAGAATTTCCTCTAGTC
TTATATGACTGTAAGTTTGAAAATGTCAAGTGGATCTATGACCAGGAGGCTCAGGAGTTCAATATTACC
CACCTACAACAACTGTGGGCTAATCATGCTGTCAAAACTCACATGTTGTATAGTATGCTACAAGGACTG
GACACTGTTCCAGTACCCTGTGGAATAGGACCAAAGATGGATGGAATGACAGAATGGGGAAATGTTAAG
CCCTCTGTCATAAAGCAGACCAGTGCCTTTGTAGAAGGAGTGAAGATGCGCACATATAAGCCCCTCATG
GACCGTCCTAAATGCCAAGGACTGGAATCCCGGATCCAGCATTTTGTACGTAGGGGACGAATTGAGCAC
CCACATTTATTCCATGAGGAAGAAACAAAAGCCAAAAGGGACTGTAATGACACACTAGAGGAAGAGAAT
ACTAATTTGGAGACACCAACGAAGAGGGTCTGTGTTGACACAGAAATTAAAAGCATCATTTAA

Restriction Sites SgfI-MluI     
ACCN NM_001271985
Insert Size 822 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001271985.1
RefSeq Size 1438 bp
RefSeq ORF 822 bp
Locus ID 83480
UniProt ID Q9BZE2
MW 31.6 kDa
Gene Summary The protein encoded by this gene catalyzes the formation of tRNA pseudouridine from tRNA uridine at position 39 in the anticodon stem and loop of transfer RNAs. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]
Transcript Variant: This variant (2) lacks an alternate coding exon and uses a downstream start codon compared to variant 1. It encodes isoform 2, which has a shorter N-terminus compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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