HIRIP3 (NM_001197323) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331200 representing NM_001197323. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCGCGGGAGAAGGAGATGCAGGAGTTCACCCGTAGCTTCTTCCGAGGCCGCCCGGACCTCAGCACG CTTACGCATTCCATCGTGCGGCGGAGGTACTTAGCTCACTCGGGCCGCAGCCACCTGGAGCCCGAGGAG AAGCAGGCACTGAAGCGGCTGGTGGAGGAGGAGCTGCTGAAGATGCAGGTGGATGAAGCCGCTTCCAGG GAAGACAAACTGGACCTTACCAAGAAGGGCAAGAGGCCTCCCACCCCTTGTAGCGACCCGGAGAGAAAA AGGTTCCGCTTCAATTCAGAGTCGGGCTGGCTCAGGTCGCCGTGGAGAGGACCACCCGGCTGTGATGAG GCTGAAGCGCTACATTCGGGCCTGTGGTGCCCATCGAAACTACAAGAAGCTGTTGGGCTCCTGTTGCTC ACACAAGGAGCGCCTGAGTATCCTCCGGGCAGAACTGGAAGCGCTAGGCATGAAGGGTACCCCTTCCCT AGGGAAGTGTCGGGCCCTGAAGGAGCAGAGGGAGGAGGCAGCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001197323
Insert Size	528 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001197323.1
RefSeq Size	2127 bp
RefSeq ORF	528 bp
Locus ID	8479
UniProt ID	Q9BW71
MW	19.7 kDa
Gene Summary	The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Aug 2011] Transcript Variant: This variant (2) lacks an exon in the coding region, resulting in frame-shift, compared to variant 1. The resulting isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1.

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