PI4KB (NM_001198774) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NPIK; PI4K-BETA; PI4K92; PI4KBETA; PI4KIII; PI4KIIIBETA; PIK4CB
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331221 representing NM_001198774. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGGAGATACAGTAGTGGAGCCTGCCCCCTTGAAGCCAACTTCTGAGCCCACTTCTGGCCCACCAGGG AATAATGGGGGGTCCCTGCTAAGTGTCATCACGGAGGGGGTCGGGGAACTATCAGTGATTGACCCTGAG GTGGCCCAGAAGGCCTGCCAGGAGGTGTTGGAGAAAGTCAAGCTTTTGCATGGAGGCGTGGCAGTCTCT AGCAGAGGCACCCCACTGGAGTTGGTCAATGGGGATGGTGTGGACAGTGAGATCCGTTGCCTAGATGAT CCACCTGCCCAGATCAGGGAGGAGGAAGATGAGATGGGGGCCGCTGTGGCCTCAGGCACAGCCAAAGGA GCAAGAAGACGGCGGCAGAACAACTCAGCTAAACAGTCTTGGCTGCTGAGGCTGTTTGAGTCAAAACTG TTTGACATCTCCATGGCCATTTCATACCTGTATAACTCCAAGGAGCCTGGAGTACAAGCCTACATTGGC AACCGGCTCTTCTGCTTTCGCAACGAGGACGTGGACTTCTATCTGCCCCAGTTGCTTAACATGTACATC CACATGGATGAGGACGTGGGTGATGCCATTAAGCCCTACATAGTCCACCGTTGCCGCCAGAGCATTAAC TTTTCCCTCCAGTGTGCCCTGTTGCTTGGGGCCTATTCTTCAGACATGCACATTTCCACTCAACGACAC TCCCGTGGGACCAAGCTACGGAAGCTGATCCTCTCAGATGAGCTAAAGCCAGCTCACAGGAAGAGGGAG CTGCCCTCCTTGAGCCCGGCCCCTGACACAGGGCTGTCTCCCTCCAAAAGGACTCACCAGCGCTCTAAG TCAGATGCCACTGCCAGCATAAGTCTCAGCAGCAACCTGAAACGAACAGCCAGCAACCCTAAAGTGGAG AATGAGGATGAGCCTGTTCGACTGGCTCCTGAGAGAGAATTCATCAAGTCCCTGATGGCGATCGGCAAG CGGCTGGCCACGCTCCCCACCAAAGAGCAGAAAACACAGAGGCTGATCTCAGAGCTCTCCCTGCTCAAC CATAAGCTCCCTGCCCGAGTCTGGCTGCCCACTGCTGGCTTTGACCACCACGTGGTCCGTGTACCCCAC ACACAGGCTGTTGTCCTCAACTCCAAGGACAAGGCTCCCTACCTGATTTATGTGGAAGTCCTTGAATGT GAAAACTTTGACACCACCAGTGTCCCTGCCCGGATCCCCGAGAACCGAATTCGGAGTACGAGGTCCGTA GAAAACTTGCCCGAATGTGGTATTACCCATGAGCAGCGAGCTGGCAGCTTCAGCACTGTGCCCAACTAT GACAACGATGATGAGGCCTGGTCGGTGGATGACATAGGCGAGCTGCAAGTGGAGCTCCCCGAAGTGCAT ACCAACAGCTGTGACAACATCTCCCAGTTCTCTGTGGACAGCATCACCAGCCAGGAGAGCAAGGAGCCT GTGTTCATTGCAGCAGGGGACATCCGCCGGCGCCTTTCGGAACAGCTGGCTCATACCCCGACAGCCTTC AAACGAGACCCAGAAGATCCTTCTGCAGTTGCTCTCAAAGAGCCCTGGCAGGAGAAAGTACGGCGGATC AGAGAGGGCTCCCCCTACGGCCATCTCCCCAATTGGCGGCTCCTGTCAGTCATTGTCAAGTGTGGGGAT GACCTTCGGCAAGAGCTTCTGGCCTTTCAGGTGTTGAAGCAACTGCAGTCCATTTGGGAACAGGAGCGA GTGCCCCTTTGGATCAAGCCATACAAGATTCTTGTGATTTCGGCTGATAGTGGCATGATTGAACCAGTG GTCAATGCTGTGTCCATCCATCAGGTGAAGAAACAGTCACAGCTCTCCTTGCTCGATTACTTCCTACAG GAGCACGGCAGTTACACCACTGAGGCATTCCTCAGTGCACAGCGCAATTTTGTGCAAAGTTGTGCTGGG TACTGCTTGGTCTGCTACCTGCTGCAAGTCAAGGACAGACACAATGGGAATATCCTTTTGGACGCAGAA GGCCACATCATCCACATCGACTTTGGCTTCATCCTCTCCAGCTCACCCCGAAATCTGGGCTTTGAGACG TCAGCCTTTAAGCTGACCACAGAGTTTGTGGATGTGATGGGCGGCCTGGATGGCGACATGTTCAACTAC TATAAGATGCTGATGCTGCAAGGGCTGATTGCCGCTCGGAAACACATGGACAAGGTGGTGCAGATCGTG GAGATCATGCAGCAAGGTTCTCAGCTTCCTTGCTTCCATGGCTCCAGCACCATTCGAAACCTCAAAGAG AGGTTCCACATGAGCATGACTGAGGAGCAGCTGCAGCTGCTGGTGGAGCAGATGGTGGATGGCAGTATG CGGTCTATCACCACCAAACTCTATGACGGCTTCCAGTACCTCACCAACGGCATCATGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001198774
Insert Size	2406 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001198774.1
RefSeq Size	3934 bp
RefSeq ORF	2406 bp
Locus ID	5298
UniProt ID	Q9UBF8
Protein Families	Druggable Genome
Protein Pathways	Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
MW	89.8 kDa
Gene Summary	Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks two separated exons in the 5' region, as compared to variant 2. The resulting isoform (2) has a shorter N-terminus and lacks an internal segment, as compared to isoform 1. Variants 2 and 3 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.