BRCAA1 (ARID4B) (NM_001206794) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BCAA; BRCAA1; RBBP1L1; RBP1L1; SAP180
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331682 representing NM_001206794. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAGGCCCTTGATGAGCCTCCCTATTTGACAGTGGGCACTGATGTGAGTGCTAAATACAGAGGAGCC TTTTGTGAAGCCAAGATCAAGACAGCAAAAAGACTTGTCAAAGTCAAGGTGACATTTAGACATGATTCT TCAACAGTGGAAGTTCAGGATGACCACATAAAGGGCCCACTAAAGGTAGGAGCTATTGTGGAAGTGAAG AATCTTGATGGTGCATATCAGGAAGCTGTTATCAATAAACTAACAGATGCGAGTTGGTACACTGTAGTT TTTGATGACGGAGATGAGAAGACACTGAGACGATCTTCACTGTGCCTGAAAGGAGAGAGGCATTTTGCT GAAAGTGAAACATTAGACCAGCTCCCACTCACCAACCCTGAGCATTTTGGCACTCCAGTCATAGGAAAG AAAACAAATAGAGGAAGAAGATCTAATCATATACCAGAGGAAGAGTCTTCATCATCCTCCAGTGATGAA GATGAGGATGATAGGAAACAGATTGATGAGCTACTAGGCAAAGTTGTATGTGTAGATTACATTAGTTTG GATAAAAAGAAAGCACTGTGGTTTCCTGCATTGGTGGTTTGTCCTGATTGTAGTGATGAGATTGCTGTA AAAAAGGACAATATTCTTGTTCGATCTTTCAAAGATGGAAAATTTACTTCAGTTCCAAGAAAAGATGTC CATGAAATTACTAGTGACACTGCACCAAAGCCTGATGCTGTTTTAAAGCAAGCCTTTGAACAGGCACTT GAATTTCACAAAAGTAGAACTATTCCTGCTAACTGGAAGACTGAATTGAAAGAAGATAGCTCTAGCAGT GAAGCAGAGGAAGAAGAGGAGGAGGAAGATGATGAAAAAGAAAAGGAGGATAATAGCAGTGAAGAAGAA GAAGAAATAGAACCATTTCCAGAAGAAAGGGAGAACTTTCTTCAGCAATTGTACAAATTTATGGAAGAT AGAGGTACACCTATTAACAAACGACCTGTACTTGGATATCGAAATTTGAATCTCTTTAAGTTATTCAGA CTTGTACACAAACTTGGAGGATTTGATAATATTGAAAGTGGAGCTGTTTGGAAACAAGTCTACCAAGAT CTTGGAATCCCTGTCTTAAATTCAGCTGCAGGATACAATGTTAAATGTGCTTATAAAAAATACTTATAT GGTTTTGAGGAGTACTGTAGATCAGCCAACATTGAATTTCAGATGGCATTGCCAGAGAAAGTTGTTAAC AAGCAATGTAAGGAGTGTGAAAATGTAAAAGAAATAAAAGTTAAGGAGGAAAATGAAACAGAGATCAAA GAAATAAAGATGGAGGAGGAGAGGAATATAATACCAAGAGAAGAAAAGCCTATTGAGGATGAAATTGAA AGAAAAGAAAATATTAAGCCCTCTCTGGGAAGTAAAAAGAATTTATTAGAATCTATACCTACACATTCT GATCAGGAAAAAGAAGTTAACATTAAAAAACCAGAAGACAATGAAAATCTGGATGACAAAGATGATGAC ACAACTAGGGTAGATGAATCCCTCAACATAAAGGTAGAAGCTGAGGAAGAAAAAGCAAAATCTGGAGAT GAAACGAATAAAGAAGAAGATGAAGATGATGAAGAAGCAGAAGAGGAGGAGGAGGAGGAAGAAGAAGAA GAGGATGAAGATGATGATGACAACAATGAGGAAGAGGAGTTTGAGTGCTATCCACCAGGCATGAAAGTC CAAGTGCGGTATGGACGAGGGAAAAATCAAAAAATGTATGAAGCTAGTATTAAAGATTCTGATGTCGAA GGTGGAGAGGTCCTTTACTTGGTGCATTACTGCGGATGGAATGTGAGATACGATGAATGGATTAAAGCA GATAAAATAGTAAGACCTGCTGATAAAAATGTGCCAAAGATAAAACATCGGAAGAAAATAAAGAATAAA TTAGACAAAGAAAAAGACAAAGATGAAAAATACTCTCCAAAAAACTGTAAACTTCGGCGCTTGTCCAAA CCACCATTTCAGACAAATCCATCTCCTGAAATGGTATCCAAACTGGATCTCACTGATGCCAAAAACTCT GATACTGCTCATATTAAGTCCATAGAAATTACTTCGATCCTTAATGGACTTCAAGCTTCTGAAAGTTCT GCTGAAGACAGTGAGCAGGAAGATGAGAGAGGTGCTCAAGACATGGATAATAATGGCAAAGAGGAATCT AAGATTGATCATTTGACCAACAACAGAAATGATCTTATTTCAAAGGAGGAACAGAACAGTTCATCTTTG CTAGAAGAAAACAAAGTTCATGCAGATTTGGTAATATCCAAACCAGTGTCAAAATCTCCAGAAAGATTA AGGAAAGATATAGAAGTATTATCCGAAGATACTGATTATGAAGAAGATGAAGTCACAAAAAAGAGAAAG GATGTCAAGAAGGACACAACAGATAAATCTTCAAAACCACAAATAAAACGTGGTAAAAGAAGGTATTGC AATACAGAAGAGTGTCTAAAAACTGGATCACCTGGCAAAAAGGAAGAGAAGGCCAAGAACAAAGAATCA CTTTGCATGGAAAACAGTAGCAACAGCTCTTCAGATGAAGATGAAGAAGAAACAAAAGCAAAGATGACA CCAACTAAGAAATACAATGGTTTGGAGGAAAAAAGAAAATCTCTACGGACAACTGGTTTCTATTCAGGA TTTTCAGAAGTGGCAGAAAAAAGGATTAAACTTTTAAATAACTCTGATGAAAGACTTCAAAACAGCAGG GCCAAAGATCGAAAAGATGTCTGGTCAAGTATTCAGGGACAGTGGCCTAAAAAAACGCTGAAAGAGCTT TTTTCAGACTCTGATACTGAGGCTGCAGCTTCCCCACCGCATCCTGCCCCAGAGGAGGGGGTGGCAGAG GAGTCACTGCAGACTGTGGCTGAAGAGGAGAGTTGTTCACCCAGTGTAGAACTAGAAAAACCACCTCCA GTCAATGTCGATAGTAAACCCATTGAAGAAAAAACAGTAGAGGTCAATGACAGAAAAGCAGAATTTCCA AGTAGTGGCAGTAATTCAGTGCTAAATACCCCTCCTACTACACCTGAATCGCCTTCATCAGTCACTGTA ACAGAAGGCAGCCGGCAGCAGTCTTCTGTAACAGTATCAGAACCACTGGCTCCAAACCAAGAAGAGGTT CGAAGTATCAAGAGTGAAACTGATAGCACAATTGAGGTGGATAGTGTTGCTGGGGAGCTCCAAGACCTC CAGTCTGAAGGGAATAGCTCGCCAGCAGGTTTTGATGCCAGTGTGAGCTCAAGCAGTAGTAATCAGCCA GAACCAGAACATCCTGAAAAAGCCTGTACAGGTCAGAAAAGAGTGAAAGATGCTCAGGGAGGAGGAAGT TCATCAAAAAAGCAGAAAAGAAGCCATAAAGCAACAGTGGTAAACAACAAAAAGAAGGGAAAAGGCACA AATAGTAGTGATAGTGAAGAACTTTCAGCTGGTGAAAGTATAACTAAGAGTCAGCCAGTCAAATCAGTT TCCACTGGAATGAAGTCTCATAGTACCAAATCTCCCGCAAGGACGCAGTCTCCAGGAAAATGTGGAAAG AATGGTGATAAGGATCCTGATCTCAAGGAACCCAGTAATCGATTACCCAAAGTTTACAAATGGAGTTTT CAGATGTCGGACCTGGAAAATATGACAAGTGCCGAACGCATCACAATTCTTCAAGAAAAACTTCAAGAA ATCAGAAAACATTATCTGTCATTAAAATCTGAAGTAGCTTCCATTGATCGGAGGAGAAAGCGTTTAAAG AAGAAAGAGAGAGAAAGTGCTGCTACATCCTCATCCTCCTCTTCACCTTCATCCAGTTCCATAACAGCT GCTGTTATGTTAACTTTAGCTGAACCGTCAATGTCCAGCGCATCACAAAATGGAATGTCAGTTGAGTGC AGGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001206794
Insert Size	3939 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001206794.1
RefSeq Size	5954 bp
RefSeq ORF	3939 bp
Locus ID	51742
UniProt ID	Q4LE39
MW	147.8 kDa
Gene Summary	This gene encodes a protein with sequence similarity to retinoblastoma-binding protein-1. The encoded protein is a subunit of the histone deacetylase-dependant SIN3A transcriptional corepressor complex, which functions in diverse cellular processes including proliferation, differentiation, apoptosis, oncogenesis, and cell fate determination. The gene product is recognized by IgG antibody isolated from a breast cancer patient and appears to be a molecular marker associated with a broad range of human malignancies. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1 and 3 encode the same protein (isoform 1).

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