MTHFD1L (NM_001242769) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	dJ292B18.2; FTHFSDC1; MTC1THFS
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331854 representing NM_001242769. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGGCACGCGTCTGCCGCTCGTCCTGCGCCAGCTCCGCCGCCCGCCCCAGCCCCCGGGCCCTCCGCGC CGCCTCCGTGTGCCCTGTCGCGCTAGCAGCGGCGGCGGCGGAGGCGGCGGCGGTGGCCGGGAGGGCCTG CTTGGACAGCGGCGGCCGCAGGATGGCCAGGCCCGGAGCAGCTGCAGCCCCGGCGGCCGAACGCCCGCG GCGCGGGACTCCATCGTCAGAGAAGTCATTCAGAATTCAAAAGAAGTTCTAAGTTTATTGCAAGAAAAA AACCCTGCCTTCAAGCCGGTTCTTGCAATTATCCAGGCAGGTGACGACAACTTGATGCAGGAAATCAAC CAGAATTTGGCTGAGGAGGCTGGTCTGAACATCACTCACATTTGCCTCCCTCCAGATAGCAGTGAAGCC GAGATTATAGATGAAATCTTAAAGATCAATGAAGATACCAGAGTACATGGCCTTGCCCTTCAGATCTCT GAGAACTTGTTTAGCAACAAAGTCCTCAATGCCTTGAAACCAGAAAAAGATGTGGATGGAGTAACAGAC ATAAACCTGGGGAAGCTGGTGCGAGGGGATGCCCATGAATGTTTTGTTTCACCTGTTGCCAAAGCTGTA ATTGAACTTCTTGAAAAATCAGGTGTCAACCTAGATGGAAAGAAGATTTTGGTAGTGGGGGCCCATGGG TCTTTGGAAGCTGCTCTACAATGCCTGTTCCAGAGAAAAGGGTCCATGACAATGAGCATCCAGTGGAAA ACACGCCAGCTTCAAAGCAAGACGGAGTCTCGTTCTGTCACCAGGCTGGAGTGCAGGCGCGTGATCTAG
Restriction Sites	SgfI-RsrII
ACCN	NM_001242769
Insert Size	828 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242769.1
RefSeq Size	1072 bp
RefSeq ORF	828 bp
Locus ID	25902
UniProt ID	Q6UB35
Protein Pathways	Glyoxylate and dicarboxylate metabolism, Metabolic pathways, One carbon pool by folate
MW	29.8 kDa
Gene Summary	The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011] Transcript Variant: This variant (4) differs in the 3' UTR and coding sequence and uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (4) has a shorter and distinct C-terminus and lacks a single internal aa compared to isoform 1. Isoform 4 is thought to lack THF synthase activity.

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