RNF22 (TRIM3) (NM_001248007) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BERP; HAC1; RNF22; RNF97
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332130 representing NM_001248007. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAGACGATGGAGTTTTACTGTGAGGCCTGTGAGACGGCCATGTGTGGTGAGTGCCGCGCCGGGGAG CATCGTGAGCATGGCACAGTGCTGCTGAGGGATGTGGTGGAGCAGCACAAGGCGGCCCTGCAGCGCCAG CTCGAGGCTGTGCGTGGCCGATTGCCACAGCTGTCCGCAGCAATTGCCTTAGTCGGGGGCATCAGCCAG CAGCTGCAGGAGCGCAAGGCAGAGGCCCTGGCCCAGATCAGTGCAGCGTTCGAGGACCTGGAGCAAGCA CTGCAGCAGCGCAAGCAGGCTCTGGTCAGCGACCTGGAGACCATTTGTGGGGCCAAACAGAAGGTGTTG CAAAGCCAGCTGGACACACTGCGCCAGGGTCAGGAACACATCGGCAGTAGCTGCAGCTTTGCAGAGCAG GCACTGCGCCTGGGCTCGGCCCCGGAGGTGTTGCTGGTGCGCAAGCACATGCGAGAGCGGCTGGCTGCA TTGGCGGCACAGGCCTTCCCGGAGCGGCCACATGAGAATGCACAGCTGGAACTGGTCCTTGAGGTGGAC GGTCTGCGGCGATCGGTGCTCAATCTGGGCGCACTGCTCACCACGAGCGCCACTGCACACGAAACGGTG GCCACGGGAGAGGGCCTGCGCCAGGCGCTAGTGGGCCAGCCTGCCTCGCTCACTGTCACTACCAAAGAC AAGGACGGGCGGTTGGTGCGCACAGGCAGCGCTGAGCTGCGTGCAGAGATCACCGGCCCGGACGGCACG CGCCTTCCGGTGCCAGTGGTGGACCACAAGAATGGCACATATGAGCTAGTGTACACAGCGCGCACGGAA GGCGAGCTGCTCCTCTCGGTGCTGCTCTACGGACAGCCAGTGCGCGGCAGCCCCTTCCGCGTGCGTGCC CTGCGTCCGGGGGACCTGCCACCTTCCCCGGACGATGTGAAGCGCCGTGTCAAGTCCCCTGGCGGCCCC GGCAGCCATGTGCGCCAGAAGGCAGTGCGTAGGCCCAGCTCCATGTACAGCACAGGCGGCAAACGAAAG GACAACCCAATTGAGGATGAGCTCGTCTTCCGTGTTGGCAGTCGTGGAAGGGAGAAAGGTGAATTCACC AATTTACAAGGTGTGTCCGCAGCCAGCAGCGGCCGCATCGTGGTAGCAGACAGCAACAACCAGTGTATT CAGGTTTTCTCCAATGAGGGCCAGTTCAAGTTCCGTTTTGGGGTCCGAGGACGCTCACCTGGGCAGCTG CAGCGCCCCACAGGTGTGGCAGTGGACACCAATGGAGACATAATTGTGGCAGACTATGACAACCGTTGG GTCAGCATCTTCTCCCCTGAGGGCAAGTTCAAGACCAAGATTGGAGCTGGCCGCCTCATGGGCCCCAAG GGAGTGGCCGTAGACCGGAATGGACATATCATTGTGGTCGACAACAAGTCTTGCTGCGTCTTTACCTTC CAGCCCAATGGCAAACTGGTTGGCCGTTTTGGGGGCCGTGGGGCCACTGACCGCCACTTTGCAGGGCCC CATTTTGTGGCTGTGAACAACAAGAATGAAATTGTAGTAACGGACTTCCATAACCATTCAGTGAAGGTG TACAGTGCCGATGGAGAGTTCCTCTTCAAGTTTGGCTCCCATGGCGAGGGCAATGGGCAGTTCAATGCC CCCACAGGAGTAGCTGTGGACTCCAATGGAAACATCATTGTGGCTGACTGGGGCAACAGCCGCATCCAG GTATTCGACAGCTCTGGCTCCTTCCTGTCCTATATCAACACATCTGCAGAACCACTGTATGGTCCACAG GGCCTGGCACTGACCTCGGATGGCCATGTGGTGGTGGCTGATGCTGGCAACCACTGCTTTAAAGCCTAT CGCTACCTCCAGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001248007
Insert Size	1878 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001248007.1
RefSeq Size	2730 bp
RefSeq ORF	1878 bp
Locus ID	10612
UniProt ID	O75382
MW	67.7 kDa
Gene Summary	The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (5) lacks several exons, which results in translation initiation at a downstream start codon, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1.

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