THBS3 (NM_001252608) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	TSP3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332200 representing NM_001252608. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGACGCAGGAACTTCGGGGGGCCCTGGCTCTTCTCCTCCTTTGCTTTTTCACATCTGCCAGTCAG GATCTGCAGGTAATTGACCTGCTGACTGTGGGCGAGTCTCGGCAGATGGTAGCTGTGGCAGAGAAGATC CGGACAGCCTTGCTCACTGCTGGGGACATCTACCTCTTATCCACCTTCCGCCTGCCCCCCAAGCAGGGT GGTGTCCTCTTTGGCCTCTATTCTCGCCAAGACAACACTCGATGGCTGGAGGCCTCTGTTGTAGGCAAG ATCAACAAAGTGACCAATGCACTGCACTCCATTCTAGGGGAGCAGACCAAGGCGCTGGTCACCCAACTC ACCCTCTTCAACCAGATCCTGGTGGAGCTGCGGGATGATATACGAGACCAGGTGAAGGAAATGTCCCTG ATCCGAAACACCATTATGGAGTGTCAGGTGTGCGGCTTCCATGAGCAGCGTTCCCACTGCAGCCCCAAT CCCTGCTTCCGAGGTGTGGACTGCATGGAAGTGTACGAGTACCCAGGCTACCGCTGTGGGCCCTGCCCC CCTGGCCTGCAGGGCAACGGCACCCACTGCAGTGACATCAATGAGTGTGCTCACGCTGACCCCTGTTTC CCGGGCTCCAGCTGCATCAACACCATGCCCGGCTTCCACTGTGAGGCCTGTCCTCGAGGGTACAAGGGC ACACAGGTGTCTGGTGTGGGCATTGACTATGCCCGGGCCAGCAAACAGGTCTGCAATGACATCGATGAA TGCAACGATGGCAACAATGGTGGCTGTGACCCAAACTCCATCTGCACCAACACTGTGGGCTCTTTCAAG TGTGGTCCCTGCCGCCTGGGTTTCCTGGGCAACCAGAGCCAGGGCTGCCTCCCAGCCCGGACCTGCCAC AGCCCAGCCCACAGCCCCTGCCACATCCATGCTCACTGTCTCTTTGAACGCAATGGTGCAGTGTCCTGC CAGTGTAACGTGGGCTGGGCTGGGAATGGGAACGTGTGTGGGACTGACACAGACATCGATGGCTACCCA GACCAAGCACTGCCCTGCATGGACAACAACAAACACTGCAAACAGGACAACTGCCTTTTGACACCCAAC TCTGGGCAGGAAGATGCTGATAATGATGGTGTGGGGGACCAGTGTGATGATGATGCTGATGGGGATGGG ATCAAGAATGTTGAGGACAACTGCCGGCTGTTCCCCAACAAAGACCAGCAGAACTCAGATACAGATTCA TTTGGTGATGCCTGTGACAATTGCCCCAACGTTCCCAACAATGACCAGAAGGACACAGATGGCAATGGG GAAGGAGATGCCTGTGACAACGACGTGGATGGGGATGGCATCCCCAATGGATTGGACAATTGCCCTAAA GTCCCCAACCCACTACAGACAGACAGGGATGAGGACGGGGTGGGAGATGCTTGCGACAGCTGCCCTGAA ATGAGCAATCCTACCCAGACAGATGCAGACAGCGACCTGGTGGGGGATGTCTGTGATACTAATGAAGAC AGCGATGGGGATGGGCATCAGGACACCAAGGACAACTGCCCACAGCTGCCAAATAGCTCCCAGCTGGAC TCTGATAACGATGGACTTGGAGATGAGTGTGATGGGGATGATGACAATGATGGCATCCCAGATTATGTG CCTCCTGGTCCCGATAACTGCCGCCTGGTACCCAATCCCAATCAGAAGGACTCAGATGGCAATGGCGTT GGTGATGTGTGTGAGGATGACTTTGACAATGATGCTGTGGTCGACCCCCTGGATGTGTGTCCTGAAAGT GCAGAGGTAACGCTTACGGATTTTCGGGCCTATCAGACCGTCGTCCTGGATCCTGAGGGTGATGCTCAG ATTGACCCAAACTGGGTTGTGCTCAACCAGGGCATGGAAATCGTTCAGACCATGAACAGTGACCCTGGC TTGGCAGTTGGATACACGGCCTTCAATGGTGTGGACTTTGAAGGCACCTTCCATGTGAACACAGTGACT GATGATGACTACGCAGGCTTTCTCTTCAGTTATCAAGACAGTGGCCGCTTCTACGTAGTCATGTGGAAG CAGACCGAGCAGACCTACTGGCAGGCTACACCCTTCCGGGCGGTTGCCCAGCCCGGGCTGCAGCTCAAG GCAGTGACATCAGTGTCTGGCCCAGGTGAGCACCTCCGAAATGCCCTGTGGCATACTGGCCACACCCCT GATCAGGTACGACTGCTGTGGACAGACCCACGAAATGTGGGCTGGCGGGACAAGACCTCCTATCGCTGG CAGCTTCTGCACCGGCCTCAAGTTGGCTACATTCGGGTGAAGCTCTATGAGGGACCCCAGCTTGTGGCG GATTCTGGGGTGATCATTGACACATCCATGCGAGGGGGGCGTCTTGGTGTATTCTGCTTCTCCCAAGAA AACATAATTTGGTCCAATCTCCAGTATCGATGCAATGACACAGTGCCTGAGGACTTTGAGCCATTCCGG AGGCAGCTGCTCCAGGGAAGGGTGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001252608
Insert Size	2511 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252608.1
RefSeq Size	2891 bp
RefSeq ORF	2511 bp
Locus ID	7059
UniProt ID	P49746
Protein Families	Druggable Genome
Protein Pathways	ECM-receptor interaction, Focal adhesion, TGF-beta signaling pathway
MW	91.3 kDa
Gene Summary	The protein encoded by this gene belongs to the thrombospondin family. Thrombospondin family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentameric molecule linked by a single disulfide bond. This gene shares a common promoter with metaxin 1. Alternate splicing results in coding and non-coding transcript variants. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (3) lacks two in-frame exons in the coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1.

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