ALPK1 (NM_001253884) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	8430410J10Rik; LAK
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332249 representing NM_001253884. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTGTCGGAAGAGGACAAGAGCGAGGACCAGCGCTGCAGAGGCGTCCCTGAGGGCCTCCATCCTCGCT CGGGACTGTGCGGCTGCGGCGGCTATTGTGTTCTTGGTGGACCGGTTCCTGTATGGGCTCGACGTCTCT GGAAAACTTCTGCAGGTCGCCAAAGGTCTCCACAAGTTGCAGCCAGCCACGCCAATTGCCCCGCAGGTG GTTATTCGCCAAGCCCGAATCTCCGTGAACTCAGGAAAACTTTTAAAAGCAGAGTATATTCTGAGCAGT CTAATAAGCAACAATGGAGCAACGGGTACCTGGCTGTACAGAAATGAAAGTGACAAGGTCCTGGTGCAG TCGGTCTGTATACAGATCAGAGGGCAGATTCTGCAAAAGCTGGGGATGTGGTACGAAGCAGCAGAGTTA ATATGGGCCTCCATTGTAGGATATTTGGCACTTCCTCAGCCGGATAAAAAGGGCCTCTCCACGTCGCTA GGTATACTGGCAGACATCTTTGTTTCCATGAGCAAGAACGATTATGAAAAGTTTAAAAACAATCCACAA ATTAATTTGAGCCTGCTGAAGGAGTTTGACCACCATTTGCTGTCCGCTGCAGAAGCCTGCAAGCTGGCA GCTGCCTTCAGTGCCTATACGCCGCTCTTCGTGCTCACAGCTGTGAATATCCGTGGCACGTGTTTATTG TCCTACAGTAGTTCAAATGACTGTCCTCCAGAATTGAAAAACTTACATCTGTGTGAAGCCAAAGAGGCC TTTGAGATTGGCCTCCTCACCAAGAGAGATGATGAGCCTGTTACTGGAAAACAGGAGCTTCACAGCTTT GTCAAAGCTGCTTTCGGTCTCACCACAGTGCACAGAAGGCTCCATGGGGAGACAGGGACGGTCCATGCA GCAAGTCAGCTCTGTAAGGAAGCAATGGGGAAGCTGTACAATTTCAGCACTTCCTCCAGAAGTCAGGAC AGAGAAGCTCTGTCTCAAGAAGTTATGTCTGTGATTGCCCAGGTGAAGGAACATTTACAAGTTCAAAGC TTCTCAAATGTAGATGACAGATCTTATGTTCCCGAGAGTTTCGAGTGCAGGTTGGATAAACTTATCTTG CATGGGCAAGGGGATTTCCAAAAAATCCTTGACACCTATTCACAGCACCATACTTCGGTGTGTGAAGTA TTTGAAAGTGATTGTGGAAACAACAAAAATGAACAGAAAGATGCAAAAACAGGAGTCTGCATCACTGCT CTAAAAACAGAAATAAAAAACATAGATACTGTGAGTACTACTCAAGAAAAGCCACATTGTCAAAGAGAC ACAGGAATATCTTCCTCCCTAATGGGTAAGAATGTTCAGAGGGAACTCAGAAGGGGAGGAAGGAGAAAC TGGACCCATTCTGATGCATTTCGAGTCTCCTTGGATCAAGATGTGGAGACTGAGACTGAGCCATCGGAC TACAGCAATGGTGAGGGAGCTGTTTTCAACAAGTCTCTGAGTGGCAGCCAGACTTCCAGTGCTTGGAGC AACTTATCAGGGTTTAGTTCCTCTGCAAGCTGGGAGGAAGTGAATTATCACGTTGACGACAGGTCAGCC AGAAAAGAGCCTGGCAAAGAACATCTGGTGGACACTCAGTGTTCCACTGCCTTGTCTGAGGAGCTAGAG AATGACAGGGAAGGCAGAGCTATGCATTCATTGCATTCACAGCTTCATGATCTCTCTCTTCAGGAACCC AACAATGACAATTTGGAGCCTTCTCAAAATCAGCCACAGCAACAGATGCCCTTGACACCCTTCTCGCCT CATAATACCCCAGGCATTTTCTTGGCCCCTGGTGCAGGGCTTCTAGAAGGAGCTCCAGAAGGTATCCAG GAAGTCAGAAATATGGGACCCAGAAATACTTCTGCTCACTCCAGACCCTCATATCGTTCTGCTTCTTGG TCTTCTGATTCTGGTAGGCCCAAGAATATGGGCACACATCCTTCAGTCCAAAAAGAAGAAGCCTTTGAA ATAATTGTTGAGTTTCCAGAAACCAACTGCGATGTCAAAGACAGGCAGGGGAAAGAGCAGGGAGAAGAA ATTAGTGAAAGAGGCGCAGGCCCTACATTTAAAGCTAGTCCCTCCTGGGTTGACCCAGAAGGAGAAACA GCAGAAAGCACTGAAGATGCACCCTTAGACTTTCACAGGGTCCTGCACAATTCTCTGGGAAACATTTCC ATGCTGCCATGTAGCTCCTTCACCCCTAATTGGCCTGTTCAAAATCCTGACTCCAGAAAAAGTGGTGGC CCAGTCGCAGAGCAGGGCATCGACCCTGATGCCTCCACAGTGGATGAGGAGGGGCAACTGCTCGACAGC ATGGATGTTCCCTGCACAAATGGGCACGGCTCTCATAGACTGTGCATTCTGAGACAGCCGCCTGGTCAG AGGGCGGAGACCCCCAATTCCTCTGTAAGCGGTAACATCCTCTTCCCTGTCCTCAGCGAGGACTGCACT ACCACAGAGGAAGGAAATCAGCCTGGAAACATGCTAAACTGCAGCCAGAACTCCAGCTCATCCTCAGTG TGGTGGCTGAAATCACCTGCATTTTCCAGTGGTTCTTCTGAGGGGGACAGCCCTTGGTCCTATCTGAAT TCCAGTGGGAGTTCTTGGGTTTCATTGCCGGGAAAGATGAGGAAAGAGATCCTTGAGGCTCGCACCTTG CAACCTGATGACTTTGAAAAGCTGTTGGCAGGAGTGAGGCATGATTGGCTGTTTCAGAGACTAGAGAAT ACGGGGGTTTTTAAGCCCAGTCAACTCCACCGAGCACATAGTGCTCTTTTGTTAAAATATTCAAAAAAA TCTGAACTGTGGACGGCCCAGGAAACTATTGTCTATTTGGGGGACTACTTGACTGTGAAGAAAAAAGGC AGACAAAGAAATGCTTTTTGGGTTCATCATCTTCATCAAGAAGAAATTCTGGGGAGGTATGTTGGGAAA GACTATAAGGAGCAGAAGGGGCTCTGGCACCACTTCACTGATGTGGAGCGACAGATGACCGCACAGCAC TATGTGACAGAATTTAACAAGAGACTCTATGAACAAAACATTCCCACCCAGATATTCTACATCCCATCC ACAATACTACTGATTTTAGAGGACAAGACAATAAAGGGATGTATCAGTGTGGAGCCTTACATACTGGGA GAATTTGTAAAATTGTCAAATAACACGAAAGTGGTGAAAACAGAATACAAAGCCACAGAATATGGCTTG GCCTATGGCCATTTTTCTTATGAGTTTTCTAATCATAGAGATGTTGTGGTCGATTTACAAGGTTGGGTA ACCGGTAATGGAAAAGGACTCATCTACCTCACAGATCCCCAGATTCACTCCGTTGATCAGAAAGTTTTC ACTACCAATTTTGGAAAGAGAGGAATTTTTTACTTCTTTAATAACCAGCATGTGGAATGTAATGAAATC TGCCATCGTCTTTCTTTGACTAGACCTTCAATGGAGAAACCATGCACATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001253884
Insert Size	3501 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253884.1
RefSeq Size	5234 bp
RefSeq ORF	3501 bp
Locus ID	80216
UniProt ID	Q96QP1
Protein Families	Druggable Genome, Protein Kinase
MW	130 kDa
Gene Summary	This gene encodes an alpha kinase. Mice which were homozygous for disrupted copies of this gene exhibited coordination defects (PMID: 21208416). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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