Nicotinic Acetylcholine Receptor alpha 4 (CHRNA4) (NM_001256573) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BFNC; EBN; EBN1; NACHR; NACHRA4; NACRA4
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332422 representing NM_001256573. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAATTCGGCTCCTGGACCTACGACAAGGCCAAGATCGACCTGGTGAACATGCACAGCCGCGTGGAC CAGCTGGACTTCTGGGAGAGTGGCGAGTGGGTCATCGTGGATGCCGTGGGCACCTACAACACCAGGAAG TACGAGTGCTGTGCCGAGATCTACCCGGACATCACCTATGCCTTCGTCATCCGGCGGCTGCCGCTCTTC TACACCATCAACCTCATCATCCCCTGCCTGCTCATCTCCTGCCTCACCGTGCTGGTCTTCTACCTGCCC TCCGAGTGTGGCGAGAAGATCACGCTGTGCATCTCCGTGCTGCTGTCGCTCACCGTCTTCCTGCTGCTC ATCACCGAGATCATCCCGTCCACCTCACTGGTCATCCCACTCATCGGCGAGTACCTGCTGTTCACCATG ATCTTCGTCACCCTGTCCATCGTCATCACGGTCTTCGTGCTCAACGTGCACCACCGCTCGCCACGCACG CACACCATGCCCACCTGGGTACGCAGGGTCTTCCTGGACATCGTGCCACGCCTGCTCCTCATGAAGCGG CCGTCCGTGGTCAAGGACAATTGCCGGCGGCTCATCGAGTCCATGCATAAGATGGCCAGTGCCCCGCGC TTCTGGCCCGAGCCAGAAGGGGAGCCCCCTGCCACGAGCGGCACCCAGAGCCTGCACCCGCCCTCACCG TCCTTCTGTGTCCCCCTGGATGTGCCGGCTGAGCCTGGGCCTTCCTGCAAGTCACCCTCCGACCAGCTC CCTCCTCAGCAGCCCCTGGAAGCTGAGAAAGCCAGCCCCCACCCCTCGCCTGGACCCTGCCGCCCGCCC CACGGCACCCAGGCACCAGGGCTGGCCAAAGCCAGGTCCCTCAGCGTCCAGCACATGTCCAGCCCTGGC GAAGCGGTGGAAGGCGGCGTCCGGTGCCGGTCTCGGAGCATCCAGTACTGTGTTCCCCGAGACGATGCC GCCCCCGAGGCAGATGGCCAGGCTGCCGGCGCCCTGGCCTCTCGCAACACCCACTCGGCTGAGCTCCCA CCCCCAGACCAGCCCTCTCCGTGCAAATGCACATGCAAGAAGGAGCCCTCTTCGGTGTCCCCGAGCGCC ACGGTCAAGACCCGCAGCACCAAAGCGCCGCCCCCGCACCTGCCCCTGTCGCCGGCCCTGACCCGGGCG GTGGAGGGCGTCCAGTACATTGCAGACCACCTGAAGGCCGAAGACACAGACTTCTCGGTGAAGGAGGAC TGGAAGTACGTGGCCATGGTCATCGACCGCATCTTCCTCTGGATGTTCATCATCGTCTGCCTGCTGGGG ACGGTGGGCCTCTTCCTGCCGCCCTGGCTGGCTGGCATGATCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001256573
Insert Size	1356 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256573.1
RefSeq Size	5474 bp
RefSeq ORF	1356 bp
Locus ID	1137
UniProt ID	P43681
Protein Families	Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
MW	49.9 kDa
Gene Summary	This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012] Transcript Variant: This variant (2) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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