TSGA14 (CEP41) (NM_001257159) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	JBTS15; TSGA14
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332509 representing NM_001257159. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCCCTCCGGAGGCACATTGGGAACCCTGAGTATCTGATGAAAAGGATACCACAGAACCCAAGATAC CAGCATATCAAATCAAGACTGGACACTGATTATAGATACAAAAAAGATGAGCTTTTCAAGAGACTAAAA GTTACAACTTTTGCCCAGCTGATCATCCAAGTTGCTTCCCTCTCTGATCAAACACTGGAAGTGACAGCT GAGGAGATTCAAAGGCTGGAAGACAATGATTCTGCAGCTTCAGACCCTGATGCTGAAACCACTGCCAGG ACCAATGGGAAAGGAAATCCAGGTGAGCAGTCGCCGAGCCCTGAGCAGTTCATAAACAACGCAGGAGCA GGGGACTCCAGCCGCTCAACTCTTCAGAGTGTCATCAGTGGTGTTGGGGAACTGGATCTAGACAAAGGG CCAGTGAAGAAAGCAGAGCCCCATACCAAAGACAAACCTTATCCTGACTGCCCCTTCCTGCTGCTAGAT GTGCGTGATAGAGATTCTTACCAGCAGTGCCACATTGTTGGAGCTTACAGTTACCCAATTGCAACTCTG TCTAGAACAATGAACCCTTATTCAAATGATATTCTTGAATATAAAAATGCCCATGGCAAGATCATCATT CTGTATGACGATGATGAAAGGCTGGCCAGTCAGGCGGCCACCACCATGTGCGAGCGTGGATTTGAAAAC CTCTTCATGCTTTCCGGAGGCCGACTGAACCAAGCTAACTCCTCCGGAAGAGAGTCCAAGGTGCCTGGT GCCCGAAGCGCTCAGAATCTGCCAGGTGGCGGCCCCGCCAGCCACTCAAACCCCCGCTCCCTCAGCAGT GGTCACCTGCAAGGCAAACCCTGGAAGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001257159
Insert Size	858 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001257159.1
RefSeq Size	6222 bp
RefSeq ORF	858 bp
Locus ID	95681
UniProt ID	Q9BYV8
Protein Families	Druggable Genome
MW	31.6 kDa
Gene Summary	This gene encodes a centrosomal and microtubule-binding protein which is predicted to have two coiled-coil domains and a rhodanese domain. In human retinal pigment epithelial cells the protein localized to centrioles and cilia. Mutations in this gene have been associated with Joubert Syndrome 15; an autosomal recessive ciliopathy and neurological disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (3) lacks two in-frame exons in the coding region, compared to variant 1, which results in a shorter isoform (3), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.