POLR3E (NM_001258034) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C37; RPC5; SIN
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332587 representing NM_001258034. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCCAATGAAGAGGATGACCCAGTTGTACAGGAGATCAAGCCCAAGCAGCAGAAGGTAGAGCTTGAG ATGGCCATCGACACCCTGAACCCCAACTATTGCCGCAGCAAAGGGGAGCAGATTGCGCTGAACGTGGAC GGGGCCTGCGCCGACGAGACCAGCACGTATTCCTCGAAGCTGATGGACAAGCAGACCTTCTGCTCTTCC CAGACCACCAGTAACACATCCCGTTATGCCGCTGCACTCTACAGGCAAGGTGAGCTCCACCTGACACCT TTACATGGCATCCTGCAGCTGCGGCCCAGCTTCTCCTACCTGGATAAGGCTGACGCCAAGCACCGGGAG AGGGAGGCGGCCAACGAGGCAGGGGACTCTTCACAGGATGAGGCGGAAGACGATGTTAAGCAGATCACG GTGCGGTTCTCCCGGCCGGAGTCAGAGCAGGCCCGCCAGCGCCGTGTGCAGTCCTATGAGTTCCTGCAG AAGAAGCACGCAGAGGAGCCCTGGGTCCACCTGCATTACTATGGCCTGAGGGACAGTCGCTCTGAGCAT GAGCGTCAGTACCTGCTGTGCCCCGGCTCAAGCGGGGTGGAGAACACGGAGCTCGTCAAGTCACCCAGT GAGTACCTGATGATGCTGATGCCACCCAGCCAGGAGGAGGAGAAAGACAAGCCTGTGGCCCCCAGCAAC GTCCTGTCGATGGCCCAGCTGCGCACGCTGCCCCTGGCCGATCAGATCAAGATCCTGATGAAGAATGTG AAGGTCATGCCTTTTGCCAACTTGATGAGCCTCCTGGGCCCCTCCATCGATTCCGTGGCTGTTCTGCGG GGCATCCAGAAGGTGGCGATGTTGGTCCAAGGGAACTGGGTGGTGAAGAGTGACATCCTATACCCCAAG GACTCGTCCAGCCCTCACAGCGGCGTGCCTGCTGAGGTGCTCTGCAGGGGCCGAGACTTCGTTATGTGG AAGTTCACGCAGAGCCGCTGGGTGGTTAGGAAAGAGGTGGCAACCGTGACCAAACTCTGCGCCGAGGAT GTGAAGGACTTCCTGGAGCACATGGCCGTGGTGAGGATCAACAAAGGCTGGGAGTTCATTCTGCCTTAT GATGGGGAGTTCATCAAGAAGCACCCGGATGTGGTCCAGCGGCAGCACATGCTGTGGACGGGTATCCAG GCCAAACTGGAAAAAGTCTATAATCTTGTAAAGGAAACCATGCCAAAGAAGCCGGATGCACAATCAGGG CCTGCCGGGCTGGTCTGTGGGGACCAGCGGATCCAAGTAGCCAAAACCAAGGCCCAGCAGAACCACGCG TTGCTGGAGCGGGAGCTGCAGCGGCGGAAGGAGCAGCTGCGGGTGCCTGCGGTCCCGCCCGGTGTGCGG ATCAAGGAGGAGCCCGTGAGCGAGGAGGGCGAGGAGGACGAGGAGCAGGAGGCGGAGGAGGAGCCCATG GACACTTCCCCCAGCGGCCTCCACAGCAAGCTGGCCAACGGGCTGCCTCTCGGGCGGGCTGCGGGCACA GACAGCTTCAACGGGCACCCGCCCCAGGGCTGCGCCAGCACCCCTGTGGCTCGGGAACTGAAGGCCTTC GTGGAGGCCACCTTTCAGAGACAGTTTGTGCTCACGCTGAGCGAACTCAAGCGCCTCTTCAATCTGCAC TTGGCCAGCCTGCCCCCCGGCCACACACTCTTCAGCGGCATCTCGGACCGCATGCTACAGGACACGGTG CTGGCCGCCGGTTGCAAGCAGATACTGGTGCCTTTTCCCCCCCAGACTGCTGCTTCCCCGGATGAGCAG AAGGTGTTTGCCCTCTGGGAGTCTGGAGACATGAGTGATCAGCATCGACAGGTTTTGCTTGAAATTTTT TCCAAAAATTACCGGGTACGCCGAAACATGATCCAGTCTCGGTTGACTCAAGAGTGTGGAGAAGATCTC AGTAAACAGGAGGTGGATAAAGTACTAAAGGACTGCTGTGTAAGCTATGGTGGCATGTGGTACCTTAAA GGGACAGTACAGTCTTGA
Restriction Sites	SgfI-NotI
ACCN	NM_001258034
Insert Size	2019 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258034.1
RefSeq Size	3637 bp
RefSeq ORF	2019 bp
Locus ID	55718
UniProt ID	Q9NVU0
Protein Families	Transcription Factors
MW	75.8 kDa
Gene Summary	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) lacks an exon and uses an alternate acceptor splice site in the 5' region compared to variant 1, However, it maintains the reading frame, and encodes an isoform (4) missing an internal protein segment compared to isoform 1.

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